Immunofluorescent analysis of Flightless I showing staining in the cytoplasm of Raji cells. Raji cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Flightless I polyclonal antibody (Product # PA5-30032) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 981 and 1209 of Human Flightless I|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30032 targets Flightless I in IF and WB applications and shows reactivity with Human and mouse samples.
The PA5-30032 immunogen is recombinant fragment corresponding to a region within amino acids 981 and 1209 of Human Flightless I.
This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17.
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