Invitrogen

Flotillin 1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

1 Reference

View all (12) Flotillin 1 antibodies

Datasheet

Datasheet

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Flotillin 1 Antibody in Western Blot (WB) — Western blot was performed using Anti- Flotillin 1 Polyclonal Antibody (Product # PA5-17127) and a 47kDa band corresponding to Flotillin 1 was observed across all the cell lines and tissue lysates tested except THP1 which is reported negative for Flotillin 1 expression. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), A549 (Lane 6), THP-1 (Lane 7), tissue extracts of Mouse Lung (Lane 8), Mouse Brain (Lane 9) and Mouse Heart (Lane 10) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Flotillin 1 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues owing to their inherent genetic constitution. Relative expression of Flotillin 1 was observed in most of the cell lines and tissue extracts in comparison to THP-1 using Flotillin 1 Polyclonal Antibody (Product # PA5-17127) in western blot. . {RE}

Product Details

Tested Applications

Dilution

Western Blot (WB)

1:1000

Published Applications

Immunohistochemistry - Free Floating (IHC (Free))

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding residue surrounding Ile368 of human flotillin-1

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

RRID

AB_11000094

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Flotillin-1; integral membrane component of caveolae; OTTHUMP00000164855; REG-2; Reggie-2

Gene Aliases: FLOT1; Rareg; Reg2; reggie-2

UniProt ID: (Human) O75955, (Mouse) O08917, (Rat) Q9Z1E1

Entrez Gene ID: (Human) 10211, (Mouse) 14251, (Rat) 64665

Function(s)

Process(es)

protease binding protein binding ionotropic glutamate receptor binding protein heterodimerization activity

Flotillin 1 Polyclonal Antibody

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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