Immunofluorescent analysis of Frequenin using a Frequenin polyclonal antibody (Product # PA1-32140). Hippocampal neurons were prepared from 17 d old NMRI mice and grown as described previously µg. Grosse et al., (2000) J. Neurosci. 20: 1869-1882). The figure shows the distribution of neuronal calcium sensor-1 (NCS-1) (red) and synaptobrevin (green) in hippo-campal cell cultures after 19 d in vitro. Numerous synapses immunoreactive for synaptobrevin also show NCS-1 immunoreactivity. NCS-1 derived from invertebrate homologs has been referred to as frequenin.
|Tested species reactivity||Mammal|
|Host / Isotype||Chicken / IgY|
|Immunogen||Recombinant full length Frequenin (recombinant from Mouse with extensive post-translational modifications).|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32140 detects Frequenin from mammal samples.
PA1-32140 has been successfully used in ELISA, Western blot applications.
The PA1-32140 immunogen is: Recombinant full length Frequenin (recombinant from Mouse with extensive post-translational modifications).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.