Proteintech

Frizzled 8 Polyclonal Antibody

View all (12) FZD8 antibodies

Questions & Answers

Questions & Answers

Product Image Gallery

Frizzled 8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 55093-1-AP ( Frizzled 8 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Frizzled 8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 55093-1-AP ( Frizzled 8 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Frizzled 8 Antibody in Western Blot (WB) — Mouse embryo tissue were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Frizzled 8 Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Frizzled 8 Antibody in Western Blot (WB) — Mouse lung tissue were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Frizzled 8 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 55093-1-AP ( Frizzled 8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Frizzled 8 Antibody in Western Blot (WB) — WB result of 55093-1-AP (Frizzled 8 antibody).

Product Details

55093-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M sodium bicarbonate/0.1M glycine, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Target Information

Members of the Frizzled family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Frizzled 8 is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. Its expression has been reported in brain, heart, kidney, pancreas, and skeletal muscle. ESTs have been isolated from embryo, eye, head/neck, heart, kidney, liver/spleen, pancreas, prostate, and testis libraries, as well as many tumor libraries. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.

Theranostics

Liu W,Zhang L,Xuan K,Hu C,Li L,Zhang Y,Jin F,Jin Y

Mon Sep 09 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: frizzled 8, seven transmembrane spanning receptor; frizzled family receptor 8; frizzled homolog 8; Frizzled-8; Fz-8; HGNC:4046

Gene Aliases: FZ-8; Fz8; FZD8; hFZ8

UniProt ID: (Human) Q9H461, (Mouse) Q61091

Entrez Gene ID: (Human) 8325, (Mouse) 14370

Molecular Function: transmembrane signal receptor

negative regulation of transcription from RNA polymerase II promoter angiogenesis G-protein coupled receptor signaling pathway neuron differentiation T cell differentiation in thymus non-canonical Wnt signaling pathway positive regulation of JUN kinase activity positive regulation of transcription from RNA polymerase II promoter canonical Wnt signaling pathway positive regulation of protein phosphorylation signal transduction cell surface receptor signaling pathway multicellular organism development Wnt signaling pathway

Process(es)

G-protein coupled receptor activity receptor binding protein binding Wnt-protein binding PDZ domain binding ubiquitin protein ligase binding Wnt-activated receptor activity signal transducer activity transmembrane signaling receptor activity