Invitrogen

G3BP1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

6 References

View all (27) G3BP1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

G3BP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of G3BP1 was performed using 70% confluent log phase U-2 OS treated with Heat Shock (45 degree celsius, 45 minutes). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with G3BP1 Polyclonal Antibody (Product # PA5-29455) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization of G3BP1 to stress granules. Panel e represents untreated cells exhibiting cytoplasmic localization. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.

G3BP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — G3BP1 Polyclonal Antibody detects G3BP1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. G3BP1 stained by G3BP1 Polyclonal Antibody (Product # PA5-29455) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

G3BP1 Antibody in Western Blot (WB) — Knockout of G3BP1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR954538_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of G3BP1 was performed by loading 30 µg of A-431 Wild Type (Lane 1), A-431 Cas9 (Lane 2) andA-431 G3BP1 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-G3BP1 Polyclonal Antibody (Product # PA5-29455, 1:1500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:8000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to G3BP1.

G3BP1 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inG3BP1 KO cell line compared to control cell line using Anti-G3BP1 Polyclonal Antibody (Product # PA5-29455).

Product Details

PA5-29455

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 147 and 466 of Human G3BP1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2546931

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2.

Predicted reactivity: Mouse (90%), Rat (91%), Pig (94%), Chicken (82%), Rhesus Monkey (99%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of a novel compound that simultaneously impairs the ubiquitin-proteasome system and autophagy.

Autophagy

Giovannucci TA,Salomons FA,Stoy H,Herzog LK,Xu S,Qian W,Merino LG,Gierisch ME,Haraldsson M,Lystad AH,Uvell H,Simonsen A,Gustavsson AL,Vallin M,Dantuma NP

Published figure using G3BP1 polyclonal antibody (Product # PA5-29455) in Immunocytochemistry

Fri Jul 01 00:00:00 EDT 2022

Small-molecule modulators of INAVA cytosolic condensate and cell-cell junction assemblies.

The Journal of cell biology

Chang D,Luong P,Li Q,LeBarron J,Anderson M,Barrett L,Lencer WI

Published figure using G3BP1 polyclonal antibody (Product # PA5-29455) in Immunocytochemistry

Mon Sep 06 00:00:00 EDT 2021

Human Insulin Growth Factor 2 mRNA Binding Protein 2 Increases MicroRNA 33a/b Inhibition of Liver ABCA1 Expression and Alters Low-Density Apolipoprotein Levels in Mice.

Molecular and cellular biology

Yang M,Gallo-Ebert C,Hayward M,Liu W,McDonough V,Nickels JT

PA5-29455 was used in Western Blotting to support the hypothesis that IGF2BP2 has an important role in maintaining lipid homeostasis through its modulation of ABCA1 expression, as its overexpression or loss leads to dyslipidemia.

Wed Jul 29 00:00:00 EDT 2020

TDP-43, a protein central to amyotrophic lateral sclerosis, is destabilized by tankyrase-1 and -2.

Journal of cell science

McGurk L,Rifai OM,Bonini NM

PA5-29455 was used in Immunocytochemistry-immunoflourescence to study how tankyrase 1 and 2 activity modulates TDP-43 in the disease context of amyotrophic lateral sclerosis.

Tue Jun 23 00:00:00 EDT 2020

TDP-43, a protein central to amyotrophic lateral sclerosis, is destabilized by tankyrase-1 and -2.

Journal of cell science

McGurk L,Rifai OM,Bonini NM

PA5-29455 was used in Immunocytochemistry-immunoflourescence to study how tankyrase 1 and 2 activity modulates TDP-43 in the disease context of amyotrophic lateral sclerosis.

Tue Jun 23 00:00:00 EDT 2020

RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation.

Cell

Guillén-Boixet J,Kopach A,Holehouse AS,Wittmann S,Jahnel M,Schlüßler R,Kim K,Trussina IREA,Wang J,Mateju D,Poser I,Maharana S,Ruer-Gruß M,Richter D,Zhang X,Chang YT,Guck J,Honigmann A,Mahamid J,Hyman AA,Pappu RV,Alberti S,Franzmann TM

PA5-29455 was used in Immunocytochemistry-immunoflourescence to propose that condensation coupled to conformational rearrangements and heterotypic multivalent interactions may be a general principle underlying RNP granule assembly.

Thu Apr 16 00:00:00 EDT 2020

An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function.

The EMBO journal

Mateju D,Franzmann TM,Patel A,Kopach A,Boczek EE,Maharana S,Lee HO,Carra S,Hyman AA,Alberti S

PA529455 was used in immunocytochemistry to characterize the components of stress granules

Wed Jun 14 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP-1; GAP binding protein; GAP SH3 binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; MGC111040; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain binding protein 1; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP

Gene Aliases: AI849976; B430204O07; C87777; G3BP; G3BP1; HDH-VIII; mKIAA4115; RGD1310666

UniProt ID: (Human) Q13283, (Mouse) P97855

Entrez Gene ID: (Human) 10146, (Rat) 171092, (Mouse) 27041

Molecular Function: RNA metabolism protein nucleic acid metabolism protein