Invitrogen

G3BP1 Polyclonal Antibody

G3BP1 Antibody in Immunocytochemistry (ICC/IF)

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G3BP1 Antibody (PA5-29455) in ICC/IF

Immunofluorescence analysis of G3BP1 was performed using 70% confluent log phase U-2 OS treated with Heat Shock (45 degree celsius, 45 minutes). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with G3BP1 Polyclonal Antibody (Product # PA5-29455) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Seco... View More

G3BP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

G3BP1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. HeLa cells were transfected with G3BP1 specific siRNA and decrease in signal intensity was observed in Western Blot application using Anti-G3BP1 Polyclonal Antibody (Product # PA5-29455). {KD}

Product Details

PA5-29455

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human G3BP1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2546931

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2.

Predicted reactivity: Mouse (90%), Rat (91%), Pig (94%), Chicken (82%), Rhesus Monkey (99%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cytosolic stress granules relieve the ubiquitin-proteasome system in the nuclear compartment.

The EMBO journal

Xu S,Gierisch ME,Schellhaus AK,Poser I,Alberti S,Salomons FA,Dantuma NP

PA5-29455 was used in Western Blot to reveal that stress granules play an important role in the sequestration of cytosolic misfolded proteins, thereby preventing these proteins from accumulating in the nucleus, where they would otherwise infringe nuclear proteostasis.

Wed Feb 01 00:00:00 UTC 2023

Identification of a novel compound that simultaneously impairs the ubiquitin-proteasome system and autophagy.

Autophagy

Giovannucci TA,Salomons FA,Stoy H,Herzog LK,Xu S,Qian W,Merino LG,Gierisch ME,Haraldsson M,Lystad AH,Uvell H,Simonsen A,Gustavsson AL,Vallin M,Dantuma NP

Published figure using G3BP1 polyclonal antibody (Product # PA5-29455) in Immunocytochemistry

Fri Jul 01 00:00:00 UTC 2022

Small-molecule modulators of INAVA cytosolic condensate and cell-cell junction assemblies.

The Journal of cell biology

Chang D,Luong P,Li Q,LeBarron J,Anderson M,Barrett L,Lencer WI

Published figure using G3BP1 polyclonal antibody (Product # PA5-29455) in Immunocytochemistry

Mon Sep 06 00:00:00 UTC 2021

RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation.

Cell

Guillén-Boixet J,Kopach A,Holehouse AS,Wittmann S,Jahnel M,Schlüßler R,Kim K,Trussina IREA,Wang J,Mateju D,Poser I,Maharana S,Ruer-Gruß M,Richter D,Zhang X,Chang YT,Guck J,Honigmann A,Mahamid J,Hyman AA,Pappu RV,Alberti S,Franzmann TM

PA5-29455 was used in Immunocytochemistry-immunoflourescence to propose that condensation coupled to conformational rearrangements and heterotypic multivalent interactions may be a general principle underlying RNP granule assembly.

Thu Apr 16 00:00:00 UTC 2020

RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation.

Cell

Thu Apr 16 00:00:00 UTC 2020

TDP-43, a protein central to amyotrophic lateral sclerosis, is destabilized by tankyrase-1 and -2.

Journal of cell science

McGurk L,Rifai OM,Bonini NM

PA5-29455 was used in Immunocytochemistry-immunoflourescence to study how tankyrase 1 and 2 activity modulates TDP-43 in the disease context of amyotrophic lateral sclerosis.

Tue Jun 23 00:00:00 UTC 2020

TDP-43, a protein central to amyotrophic lateral sclerosis, is destabilized by tankyrase-1 and -2.

Journal of cell science

McGurk L,Rifai OM,Bonini NM

PA5-29455 was used in Immunocytochemistry-immunoflourescence to study how tankyrase 1 and 2 activity modulates TDP-43 in the disease context of amyotrophic lateral sclerosis.

Tue Jun 23 00:00:00 UTC 2020

Human Insulin Growth Factor 2 mRNA Binding Protein 2 Increases MicroRNA 33a/b Inhibition of Liver ABCA1 Expression and Alters Low-Density Apolipoprotein Levels in Mice.

Molecular and cellular biology

Yang M,Gallo-Ebert C,Hayward M,Liu W,McDonough V,Nickels JT

PA5-29455 was used in Western Blotting to support the hypothesis that IGF2BP2 has an important role in maintaining lipid homeostasis through its modulation of ABCA1 expression, as its overexpression or loss leads to dyslipidemia.

Wed Jul 29 00:00:00 UTC 2020

An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function.

The EMBO journal

Mateju D,Franzmann TM,Patel A,Kopach A,Boczek EE,Maharana S,Lee HO,Carra S,Hyman AA,Alberti S

PA529455 was used in immunocytochemistry to characterize the components of stress granules

Wed Jun 14 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP-1; GAP binding protein; GAP SH3 binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; MGC111040; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain binding protein 1; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP

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Gene Aliases: AI849976; B430204O07; C87777; G3BP; G3BP1; HDH-VIII; mKIAA4115; RGD1310666

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UniProt ID: (Human) Q13283, (Mouse) P97855

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Entrez Gene ID: (Human) 10146, (Rat) 171092, (Mouse) 27041

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Function(s)

DNA binding mRNA binding ATP-dependent DNA helicase activity ATP-dependent RNA helicase activity endonuclease activity protein binding ATP binding poly(A) RNA binding nucleotide binding nucleic acid binding RNA binding helicase activity nuclease activity hydrolase activity RNA metabolism protein nucleic acid metabolism protein

Process(es)

transport Ras protein signal transduction DNA duplex unwinding negative regulation of canonical Wnt signaling pathway nucleic acid phosphodiester bond hydrolysis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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G3BP1 Polyclonal Antibody

G3BP1 Antibody (PA5-29455) in ICC/IF

Product Details

PA5-29455

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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