Proteintech

G6PD Polyclonal Antibody

View all (32) G6PD antibodies

Questions & Answers

Questions & Answers

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G6PD Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 25413-1-AP (G6PD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

G6PD Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

G6PD Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

G6PD Antibody in Western Blot (WB) — MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

G6PD Antibody in Western Blot (WB) — MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

G6PD Antibody in Western Blot (WB) — MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

G6PD Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

G6PD Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

G6PD Antibody in Western Blot (WB) — WB result of G6PD antibody (25413-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G6PD transfected HeLa cells.

Product Details

25413-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:5,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	Assay-dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	G6PD Fusion Protein Ag21862 (1-515 aa encoded by BC000337) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.43 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MAEQVALSR TQVCGILREE LFQGDAFHQS DTHIFIIMGA SGDLAKKKIY PTIWWLFRDG LLPENTFIVG YARSRLTVAD IRKQSEPFFK ATPEEKLKLE DFFARNSYVA GQYDDAASYQ RLNSHMNALH LGSQANRLFY LALPPTVYEA VTKNIHESCM SQIGWNRIIV EKPFGRDLQS SDRLSNHISS LFREDQIYRI DHYLGKEMVQ NLMVLRFANR IFGPIWNRDN IACVILTFKE PFGTEGRGGY FDEFGIIRDV MQNHLLQMLC LVAMEKPAST NSDDVRDEKV KVLKCISEVQ ANNVVLGQYV GNPDGEGEAT KGYLDDPTVP RGSTTATFAA VVLYVENERW DGVPFILRCG KALNERKAEV RLQFHDVAGD IFHQQCKRNE LVIRVQPNEA VYTKMMTKKP GMFFNPEESE LDLTYGNRYK NVKLPDAYER LILDVFCGSQ MHFVRSDELR EAWRIFTPLL HQIELEKPKP IPYIYGSRGP TEADELMKRV GFQYEGTYKW VNPHKL (1-515 aa encoded by B C000337)

Target Information

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ABT737 reverses cisplatin resistance by targeting glucose metabolism of human ovarian cancer cells.

International journal of oncology

Xu Y,Gao W,Zhang Y,Wu S,Liu Y,Deng X,Xie L,Yang J,Yu H,Su J,Sun L

Sat Sep 01 00:00:00 EDT 2018

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Oncotarget

Gu Z,Zhang H,Li Y,Shen S,Yin X,Zhang W,Cheng R,Zhang Y,Zhang X,Chen H,Huang B,Cao Y

Tue Dec 26 00:00:00 EST 2017

Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.

Nature communications

Ma X,Wang L,Li Y,Yang D,Li T,Li F,Sun L,Wei H,He K,Yu F,Zhao D,Hu L,Xing S,Liu Z,Li K,Guo J,Yang Z,Pan X,Li A,Shi Y,Wang J,Gao P,Zhang H

Wed Nov 15 00:00:00 EST 2017

Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.

Nature communications

Ma X,Wang L,Li Y,Yang D,Li T,Li F,Sun L,Wei H,He K,Yu F,Zhao D,Hu L,Xing S,Liu Z,Li K,Guo J,Yang Z,Pan X,Li A,Shi Y,Wang J,Gao P,Zhang H

Wed Nov 15 00:00:00 EST 2017

The Essential Role of H19 Contributing to Cisplatin Resistance by Regulating Glutathione Metabolism in High-Grade Serous Ovarian Cancer.

Scientific reports

Zheng ZG,Xu H,Suo SS,Xu XL,Ni MW,Gu LH,Chen W,Wang LY,Zhao Y,Tian B,Hua YJ

Thu May 19 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: G6PD; Glucose-6-phosphate 1-dehydrogenase

Gene Aliases: G6PD; G6PD1

UniProt ID: (Human) Q16000

Entrez Gene ID: (Human) 2539

Molecular Function: dehydrogenase

glucose metabolic process pentose-phosphate shunt lipid metabolic process cholesterol biosynthetic process NADP metabolic process NADPH regeneration glutathione metabolic process pentose-phosphate shunt, oxidative branch negative regulation of protein glutathionylation response to organic cyclic compound pentose biosynthetic process substantia nigra development response to food cellular response to oxidative stress erythrocyte maturation regulation of neuron apoptotic process response to ethanol ribose phosphate biosynthetic process glucose 6-phosphate metabolic process oxidation-reduction process

Process(es)

glucose-6-phosphate dehydrogenase activity protein binding glucose binding identical protein binding protein homodimerization activity NADP binding