Western blot analysis of G6PD in whole cell lysates (15 and 25µg for human and 20µg for mouse) from human umbilical vein endothelial cells or mouse peritoneal macrophages from wildtype (+/+) and G6PD deficient (-/-) mice using a G6PD polyclonal antibody (Product # PA1-33146). Mouse peritoneal macrophages from wildtype mice were immunoprecipitated with PA1-33146 and the precipitate and supernatant blotted for G6PD.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within human G6PD.|
|Storage buffer||PBS, tris citrate|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2-10 µg/mg of lysate|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-33146 detects G6PD from human samples.
PA1-33146 has been successfully used in immunoprecipitation and Western blot applications.
The PA1-33146 immunogen is synthetic peptide corresponding to a region within human G6PD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.