|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 237 and 508 of Human G6PD|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA5-27359 targets G6PD in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-27359 immunogen is recombinant fragment corresponding to a region within amino acids 237 and 508 of Human G6PD.
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Breast cancer stem cells rely on fermentative glycolysis and are sensitive to 2-deoxyglucose treatment.
PA5-27359 was used in western blot to identify the metabolic phenotype associated with breast cancer stem cells
|Ciavardelli D,Rossi C,Barcaroli D,Volpe S,Consalvo A,Zucchelli M,De Cola A,Scavo E,Carollo R,D'Agostino D,Forlì F,D'Aguanno S,Todaro M,Stassi G,Di Ilio C,De Laurenzi V,Urbani A||Cell death and disease (5:null)||2014|
p63 isoforms regulate metabolism of cancer stem cells.
PA5-27359 was used in western blot to study the modulation of colon cancer stem cell metabolism by p63
|D'Aguanno S,Barcaroli D,Rossi C,Zucchelli M,Ciavardelli D,Cortese C,De Cola A,Volpe S,D'Agostino D,Todaro M,Stassi G,Di Ilio C,Urbani A,De Laurenzi V||Journal of proteome research (13:2120)||2014|