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Invitrogen
GABARAP Polyclonal Antibody
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FIGURE: 1 / 6
GABARAP Antibody (PA5-78366) in ICC/IF
Immunofluorescence analysis of GABARAP was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GABARAP Polyclonal Antibody (Product # PA5-78366) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Produc... View More
Product Details
PA5-78366
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GABARAP. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
2.48 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: 293T, U87-MG, SK-N-SH, IMR32, SK-N-AS
Predicted Reactivity: Mouse (100%), Rat (100%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system. It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. GABARAP (GABAA receptor-associated protein) links GABAA receptors to the cytoskeleton and may play a role in intracellular transport of GABAA receptors and its interaction with the cytoskeleton. GABARAP belongs to the MAP1 or ATG8 like family and recent studies show that MAPK15/ERK8 is acting through interaction with ATG8 family proteins to regulate autophagy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; FLJ25768; GABA(A) receptor-associated protein; GABARAP; GABARAP a; Gamma-aminobutyric acid receptor-associated protein; MGC120154; MGC120155; MM46; unnamed protein product
Gene Aliases: ATG8A; FLC3B; GABARAP; GABARAP-a; HT004; MM46
UniProt ID: (Human) O95166
Entrez Gene ID: (Human) 11337
autophagosome assembly
microtubule cytoskeleton organization
mitophagy
protein targeting
autophagy
apoptotic process
cellular response to nitrogen starvation
chemical synaptic transmission
extrinsic apoptotic signaling pathway via death domain receptors
protein transport
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
regulation of Rac protein signal transduction
autophagosome maturation
regulation of neurotransmitter receptor localization to postsynaptic specialization membrane
positive regulation of protein K48-linked ubiquitination
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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