Proteintech

GABARAPL2 Polyclonal Antibody

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GABARAPL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens).

GABARAPL2 Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

GABARAPL2 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug GABARAPL2-Specific antibody (18724-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Product Details

18724-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated

Form

Liquid

Concentration

0.32 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.

Target Information

Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Zois CE,Hendriks AM,Haider S,Pires E,Bridges E,Kalamida D,Voukantsis D,Lagerholm BC,Fehrmann RSN,den Dunnen WFA,Tarasov AI,Baba O,Morris J,Buffa FM,McCullagh JSO,Jalving M,Harris AL

Tue Jun 28 00:00:00 EDT 2022

Role of Gate-16 and Gabarap in Prevention of Caspase-11-Dependent Excess Inflammation and Lethal Endotoxic Shock.

Frontiers in immunology

Sakaguchi N,Sasai M,Bando H,Lee Y,Pradipta A,Ma JS,Yamamoto M

Mon May 03 00:00:00 EDT 2021

GSK3B induces autophagy by phosphorylating ULK1.

Experimental & molecular medicine

Ryu HY,Kim LE,Jeong H,Yeo BK,Lee JW,Nam H,Ha S,An HK,Park H,Jung S,Chung KM,Kim J,Lee BH,Cheong H,Kim EK,Yu SW

Mon Mar 01 00:00:00 EST 2021

Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.

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Stefely JA,Zhang Y,Freiberger EC,Kwiecien NW,Thomas HE,Davis AM,Lowry ND,Vincent CE,Shishkova E,Clark NA,Medvedovic M,Coon JJ,Pagliarini DJ,Mercer CA

Tue Dec 01 00:00:00 EST 2020

CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis.

Autophagy

An HK,Chung KM,Park H,Hong J,Gim JE,Choi H,Lee YW,Choi J,Mun JY,Yu SW

Tue Sep 01 00:00:00 EDT 2020

Human cytomegalovirus hijacks the autophagic machinery and LC3 homologs in order to optimize cytoplasmic envelopment of mature infectious particles.

Scientific reports

Taisne C,Lussignol M,Hernandez E,Moris A,Mouna L,Esclatine A

Thu Mar 14 00:00:00 EDT 2019

Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.

Nature immunology

Sasai M,Sakaguchi N,Ma JS,Nakamura S,Kawabata T,Bando H,Lee Y,Saitoh T,Akira S,Iwasaki A,Standley DM,Yoshimori T,Yamamoto M

Tue Aug 01 00:00:00 EDT 2017

Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.

Nature immunology

Sasai M,Sakaguchi N,Ma JS,Nakamura S,Kawabata T,Bando H,Lee Y,Saitoh T,Akira S,Iwasaki A,Standley DM,Yoshimori T,Yamamoto M

Tue Aug 01 00:00:00 EDT 2017

Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.

Scientific reports

Boeske A,Schwarten M,Ma P,Tusche M,Mötter J,Möller C,Neudecker P,Hoffmann S,Willbold D

Thu Jul 20 00:00:00 EDT 2017

Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.

Scientific reports

Boeske A,Schwarten M,Ma P,Tusche M,Mötter J,Möller C,Neudecker P,Hoffmann S,Willbold D

Thu Jul 20 00:00:00 EDT 2017

A novel approach to analyze lysosomal dysfunctions through subcellular proteomics and lipidomics: the case of NPC1 deficiency.

Scientific reports

Tharkeshwar AK,Trekker J,Vermeire W,Pauwels J,Sannerud R,Priestman DA,Te Vruchte D,Vints K,Baatsen P,Decuypere JP,Lu H,Martin S,Vangheluwe P,Swinnen JV,Lagae L,Impens F,Platt FM,Gevaert K,Annaert W

Mon Jan 30 00:00:00 EST 2017

A novel approach to analyze lysosomal dysfunctions through subcellular proteomics and lipidomics: the case of NPC1 deficiency.

Scientific reports

Tharkeshwar AK,Trekker J,Vermeire W,Pauwels J,Sannerud R,Priestman DA,Te Vruchte D,Vints K,Baatsen P,Decuypere JP,Lu H,Martin S,Vangheluwe P,Swinnen JV,Lagae L,Impens F,Platt FM,Gevaert K,Annaert W

Mon Jan 30 00:00:00 EST 2017

Alteration of autophagosomal proteins in the brain of multiple system atrophy.

Neurobiology of disease

Tanji K,Odagiri S,Maruyama A,Mori F,Kakita A,Takahashi H,Wakabayashi K

Tue Jan 01 00:00:00 EST 2013

Alteration of autophagosomal proteins in the brain of multiple system atrophy.

Neurobiology of disease

Tanji K,Odagiri S,Maruyama A,Mori F,Kakita A,Takahashi H,Wakabayashi K

Tue Jan 01 00:00:00 EST 2013

Alteration of autophagosomal proteins in the brain of multiple system atrophy.

Neurobiology of disease

Tanji K,Odagiri S,Maruyama A,Mori F,Kakita A,Takahashi H,Wakabayashi K

Tue Jan 01 00:00:00 EST 2013

Alteration of autophagosomal proteins in the brain of multiple system atrophy.

Neurobiology of disease

Tanji K,Odagiri S,Maruyama A,Mori F,Kakita A,Takahashi H,Wakabayashi K

Tue Jan 01 00:00:00 EST 2013

Defective autophagy and mTORC1 signaling in myotubularin null mice.

Molecular and cellular biology

Fetalvero KM,Yu Y,Goetschkes M,Liang G,Valdez RA,Gould T,Triantafellow E,Bergling S,Loureiro J,Eash J,Lin V,Porter JA,Finan PM,Walsh K,Yang Y,Mao X,Murphy LO

Tue Jan 01 00:00:00 EST 2013

Defective autophagy and mTORC1 signaling in myotubularin null mice.

Molecular and cellular biology

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Bioinformatics

Protein Aliases: GABA(A) receptor-associated protein like 2; GABA(A) receptor-associated protein-like 2; gamma-aminobutyric acid (GABA-A) receptor-associated protein-like 2; Gamma-aminobutyric acid receptor-associated protein-like 2; Ganglioside expression factor 2; GATE-16; GEF-2; Genera; General protein transport factor p16; Golgi-associated ATPase enhancer of 16 kDa; MAP1 light chain 3 related protein; MAP1 light chain 3-related protein

Gene Aliases: 0610012F20Rik; 2900019O08Rik; AI173605; ATG8; ATG8C; FLC3A; GABARAPL2; GATE-16; GATE16; GEF-2; GEF2

UniProt ID: (Human) P60520, (Mouse) P60521, (Rat) P60522

Entrez Gene ID: (Human) 11345, (Mouse) 93739, (Rat) 64670

Molecular Function: non-motor microtubule binding protein