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Invitrogen
GAD65 Polyclonal Antibody
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FIGURE: 1 / 5
GAD65 Antibody (PA5-77983) in IHC (P)
GAD65 Polyclonal Antibody detects GAD65 protein expression by immunohistochemical analysis. Sample: Paraffin-Embedded adult mouse retina. Green: GAD65 protein stained by GAD65 Polyclonal Antibody (Product # PA5-77983) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 mi... View More
Product Details
PA5-77983
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of mouse GAD65. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.34 mg/mL
Amount
34 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: mouse brain, rat brain
Predicted Reactivity: Dog (81%), Rhesus Monkey (80%), Bovine (81%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
GAD65 (glutamic acid decarboxylase-65) are present in autoimmune disorders such as insulin-dependent (type1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2; Glutamate decarboxylase 2 (islet); Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; glutamic acid decarboxylase 65; MGC161605; MGC161607; RP11-420F12.2
Gene Aliases: 6330404F12Rik; GAD(65); Gad-2; Gad2; GAD65
UniProt ID: (Mouse) P48320, (Rat) Q05683
Entrez Gene ID: (Mouse) 14417, (Rat) 24380
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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