|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, KLH-conjugated, corresponding to a portion of amino acids 1-100 of human GaD67.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The sequence used is 100% homologous with mouse and 94% homologous with rat GaD67.
Suggested positive control: antigen standard for GAD1 (transient overexpression lysate), SK-N-SH.
GAD67, a 67KDa protein, is a neuronal isoform of this enzyme predominantly expressed in human Brain. It consists of Pyridoxal 5 and quote;-phosphate binding site and conserved Cysteine residues which are vital for its activity and is inhibited due to phosphorylation. It has high binding affinity for the cofactor Pyridoxal-5 and quote;phosphate and its role has been implicated in normal Palate development. It is found to be associated with childhood onset schizophrenia and craniofacial malformations and has been identified as a major auto antigen and an auto-reactive T-cell target in IDDM. Deficiency of this enzyme leads to pyridoxine dependency along with epilepsy. It also might play a role in stiff main syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.