Immunofluorescent analysis of GAD67 in 293T cells. GAD67-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min and stained using a GAD67 polyclonal antibody (Product # PA5-21397) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 5 and 216 of GAD67 (Uniprot ID#Q99259)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA5-21397 targets GAD67 in IHC-P, ICC/IF, and WB applications and shows reactivity with Human, Rat and mouse samples.
The PA5-21397 immunogen is a recombinant fragment corresponding to a region within amino acids 5 and 216 of GAD67 (Uniprot ID#Q99259).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Region-specific effects of repeated ketamine administration on the presynaptic GABAergic neurochemistry in rat brain.
PA5-21397 was used in western blot to elucidate the mechanism underlying ketamine's propsychotic-like action
|Boczek T,Lisek M,Ferenc B,Wiktorska M,Ivchevska I,Zylinska L||Neurochemistry international (91:13)||2015|