|Tested species reactivity||Chicken, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, coupled to maleimide-activated KLH, corresponding to residues (C) K(216) E D P E A D Q E H A(226) of rat/mouse GAP-43.|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1-2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: embryonic nervous tissue, brain, injured nerve.
GAP-43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.