|Tested species reactivity||Dog, Chicken, Human, Mouse, Non-human primate, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 216-226 of rat/mouse GAP-43, (C)-KEDPEADQEHA coupled to maleimide-activated KLH.|
|Purification||Antigen affinity chromatography|
|Storage buffer||sodium borate|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (IHC)||1-2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1-2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||0.1-0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody does not react with xenopus samples.
Suggested positive control: embryonic nervous tissue, brain, injured nerve.
GAP-43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.