Western blot analysis of GAPDH was performed by loading 25 ug of Hela (lane 1), 293 (lane 2), HUVEC (lane 3) and PC12 (lane 4) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a GAPDH polyclonal antibody (Product # PA1-987) at a dilution of 1:3000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~36 kDa.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide: V(126) M G V N H E K Y(140)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 6 publications below|
PA1-987 detects rat GAPDH. Shares 100% sequence homology with human and mouse.
PA1-987 has been used successfully in Western blotting. By Western blot this antibody specifically detects a ~36 kDa protein representing GAPDH.
PA1-987 immunizing peptide corresponds to amino acid residues 126-140 from human GAPDH.
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The pregnane X receptor down-regulates organic cation transporter 1 (SLC22A1) in human hepatocytes by competing for ("squelching") SRC-1 coactivator.
PA1-987 was used in western blot to study down-regulation of organic cation transporter 1 (SLC22A1) in human hepatocytes by competing for ("squelching") SRC-1 coactivator by the pregane X receptor
|Hyrsova L,Smutny T,Carazo A,Moravcik S,Mandikova J,Trejtnar F,Gerbal-Chaloin S,Pavek P||British journal of pharmacology (173:1703)||2016|
An animal model for chorioamnionitis at term.
PA1-987 was used in western blot to develop an animal model for intrapartum inflammation at term.
|Dell'Ovo V,Rosenzweig J,Burd I,Merabova N,Darbinian N,Goetzl L||American journal of obstetrics and gynecology (213:387.e1)||2015|
Wnt5a and Wnt11 inhibit the canonical Wnt pathway and promote cardiac progenitor development via the Caspase-dependent degradation of AKT.
PA1-987 was used in western blot to study the regulation of the canonical Wnt pathway and its effect on cardiac progenitor development
|Bisson JA,Mills B,Paul Helt JC,Zwaka TP,Cohen ED||Developmental biology (398:80)||2015|
Intestinal fatty acid binding protein (fabp2) in Atlantic salmon (Salmo salar): Localization and alteration of expression during development of diet induced enteritis.
PA1-987 was used in western blot to study the expression of intestinal fatty acid binding proteins FABP2a1, FABP2a2 and FABPb during diet-induced enteritis in Atlantic salmon
|Venold FF,Penn MH,Thorsen J,Gu J,Kortner TM,Krogdahl A,Bakke AM||Comparative biochemistry and physiology. Part A, Molecular and integrative physiology (164:229)||2013|
Impaired ß-adrenergic responsiveness accentuates dysfunctional excitation-contraction coupling in an ovine model of tachypacing-induced heart failure.
PA1-987 was used in western blot to study the mechanism for the dysfunctional excitation-contraction coupling in heart failure
|Briston SJ,Caldwell JL,Horn MA,Clarke JD,Richards MA,Greensmith DJ,Graham HK,Hall MC,Eisner DA,Dibb KM,Trafford AW||The Journal of physiology (589:1367)||2011|
An antibody-drug conjugate targeting the endothelin B receptor for the treatment of melanoma.
PA1-987 was used in western blot to evaluate an antibody-drug conjugate for melanoma treatment
|Asundi J,Reed C,Arca J,McCutcheon K,Ferrando R,Clark S,Luis E,Tien J,Firestein R,Polakis P||Clinical cancer research : an official journal of the American Association for Cancer Research (17:965)||2011|