|Western Blot (WB)||1:500-1:5000|
|Western Blot (WB)||See 7 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Dog, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Bovine , Mouse Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide: V(4) K V G V N G F G R I G R L V(18)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-988 detects rat GAPDH in human HeLa cell lysates and mouse and rat tissues..
PA1-988 has been used successfully in Western blotting. By Western blot this antibody specifically detects a ~36 kDa protein representing GAPDH.
PA1-988 immunizing peptide corresponds to amino acid residues 4-18 from human GAPDH.
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; G3PD; G3PDH; GAPD; GAPDH; Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH
Gene Aliases: BARS-38; CDABP0047; G3PD; GAPD; GAPDH; HEL-S-162eP; OK/SW-cl.12
UniProt ID: (Human) P04406
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