Western blot analysis of GAPDH was performed by loading 25 ug of Hela (lane 1) and C6 (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a GAPDH polyclonal antibody (Product # PA1-988) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~36 kDa.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide: V(4) K V G V N G F G R I G R L V(18)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-988 detects rat GAPDH in human HeLa cell lysates and mouse and rat tissues..
PA1-988 has been used successfully in Western blotting. By Western blot this antibody specifically detects a ~36 kDa protein representing GAPDH.
PA1-988 immunizing peptide corresponds to amino acid residues 4-18 from human GAPDH.
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Registered report: A chromatin-mediated reversible drug-tolerant state in cancer cell subpopulations.
PA1-988 was used in western blot to discuss the findings of The Reproducibility Project: Cancer Biology
|Haven B,Heilig E,Donham C,Settles M,Vasilevsky N,Owen K||eLife (5:null)||2016|
Intermittent hypoxia selects for genotypes and phenotypes that increase survival, invasion, and therapy resistance.
PA1-988 was used in western blot to identify genetic alterations and corresponding stable phenotypes that develop in cancer cells following cyclic hypoxia
|Verduzco D,Lloyd M,Xu L,Ibrahim-Hashim A,Balagurunathan Y,Gatenby RA,Gillies RJ||PloS one (10:null)||2015|
Selective in vivo targeting of human liver tumors by optimized AAV3 vectors in a murine xenograft model.
PA1-988 was used in western blot to optimize rAAV3 vectors to enhance liver tropism and use rAAV3 in combination with shikonin to treat liver disease
|Ling C,Wang Y,Zhang Y,Ejjigani A,Yin Z,Lu Y,Wang L,Wang M,Li J,Hu Z,Aslanidi GV,Zhong L,Gao G,Srivastava A,Ling C||Human gene therapy (25:1023)||2014|
Calpains mediate axonal cytoskeleton disintegration during Wallerian degeneration.
PA1-988 was used in western blot to study Wallerian degeneration and the role of calpains in the cytoskeletal disintegration of axons
|Ma M,Ferguson TA,Schoch KM,Li J,Qian Y,Shofer FS,Saatman KE,Neumar RW||Neurobiology of disease (56:34)||2013|
The cannabinoid CB1 receptor antagonist rimonabant (SR141716) inhibits cell proliferation and increases markers of adipocyte maturation in cultured mouse 3T3 F442A preadipocytes.
PA1-988 was used in western blot to study the effect of rimonabant on enzyme content of mouse 3T3 F442A preadipocytes
|Gary-Bobo M,Elachouri G,Scatton B,Le Fur G,Oury-Donat F,Bensaid M||Molecular pharmacology (69:471)||2006|