Invitrogen

GAPDH Polyclonal Antibody

Name: GAPDH Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-988

Advanced Verification

17 Published Figures

44 References

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Questions & Answers

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Additional Information:

FIGURE: 1 / 20

GAPDH Antibody (PA1-988) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), HT-29 (Lane 4), C2C12 (Lane 5), PC-12 (Lane 6), U-2 OS (Lane 7) and tissue extract (30 µg lysate) of Rat Testis (Lane 8), Mouse Liver (Lane 9). The blot was probed with Anti-GAPDH Polyclonal Antibody (Product # PA1-988, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 37 kDa band c... View More

Product Image Gallery

GAPDH Antibody — FIG 1 . RNAi-based assay to identify host factors required for intracellular survival of Y. pestis . To determine whether reproducible RNAi could be achieved in RAW264.7 macrophages, cells were reverse transfected with siRNAs targeting indicated genes. (A and B) Forty-eight hours posttransfection, cells ( n = 3) were harvested for RNA isolation and qRT-PCR (data represent the level of gene expression compared to the level for the scrambled siRNA control) (A) or protein isolation for Western blot analysis (beta-actin was used as a loading control) (B). alpha-GAPDH, anti-GAPDH antibody; alpha-betaactin, anti-betaactin antibody. To demonstrate that the Y. pestis CO92 pCD1 (-) Lux PtolC bioreporter accurately represents intracellular bacterial numbers, RAW264.7 macrophages were infected with Y. pestis CO92 pCD1 (-) Lux PtolC at the indicated MOIs ( n = 12), and extracellular bacteria were killed with gentamicin. (C) Bioluminescence (in relative light units [RLU]) of intracellular bacteria was determined at 1, 4, 8, and 18 h postinfection. (D) At 18 h, cells from each MOI ( n = 3) were lysed, and bacterial numbers (CFU) were determined and compared to 18-h bioluminescence (in RLU). (E) To demonstrate that RNAi targeting specific genes could impact the intracellular survival of Y. pestis , RAW264.7 macrophages were transfected with siRNAs targeting Rab2A or COPbeta1. Forty-eight hours posttransfection, macrophages were infected with Y. pestis CO92 pCD1 (-) Lux PtolC (MOI of 10). Ex {KD}

Immunogen image

Immunogen Image GAPDH Polyclonal Antibody

Product Details

PA1-988

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

View 43 publications 43 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat

Published species

Bovine, Dog, Hamster, Human, Mouse, Non-human primate, Rat, Rhesus monkey

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide: V(4) K V G V N G F G R I G R L V(18)

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2107310

Product Specific Information

PA1-988 detects rat GAPDH in human HeLa cell lysates and mouse and rat tissues..

PA1-988 has been used successfully in Western blotting. By Western blot this antibody specifically detects a ~36 kDa protein representing GAPDH.

PA1-988 immunizing peptide corresponds to amino acid residues 4-18 from human GAPDH.

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; AAG9; aging-associated gene 9 protein; BARS-38; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPA; GAPA-1; GAPC; GAPC-1; GAPC1; GAPCP-1; GAPCp1; GAPD; GAPDH; GAPDH-2; GAPDH-A; glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12); Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12); glyceraldehyde-3-phosphate dehydrogenase type 2; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; OCAS, p38 component; Oct1 coactivator in S phase, 38 Kd component; Peptidyl-cysteine S-nitrosylase GAPDH; similar to Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA with GenBank Accession Number M33197.1; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; unnamed protein product

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Gene Aliases: BARS-38; CDABP0047; G3PD; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; OK/SW-cl.12

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UniProt ID: (Human) P04406, (Human) O14556, (Mouse) P16858, (Rat) P04797, (Rat) Q9ESV6, (Mouse) Q64467

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Entrez Gene ID: (Human) 2597, (Dog) 403755, (Dog) 476483, (Human) 26330, (Mouse) 14433, (Rat) 24383, (Rat) 66020, (Mouse) 14447

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Function(s)

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity protein binding microtubule binding oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor transferase activity aspartic-type endopeptidase inhibitor activity peptidyl-cysteine S-nitrosylase activity identical protein binding NADP binding NAD binding disordered domain specific binding molecular_function dehydrogenase

Process(es)

microtubule cytoskeleton organization positive regulation of cytokine production immune system process glucose metabolic process glycolytic process regulation of translation apoptotic process negative regulation of endopeptidase activity regulation of macroautophagy negative regulation of translation killing of cells of other organism positive regulation of type I interferon production peptidyl-cysteine S-trans-nitrosylation positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response protein stabilization defense response to fungus neuron apoptotic process killing by host of symbiont cells antimicrobial humoral immune response mediated by antimicrobial peptide cellular response to interferon-gamma oxidation-reduction process sperm motility positive regulation of glycolytic process carbohydrate metabolic process gluconeogenesis multicellular organism development response to ammonium ion spermatid development

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GAPDH Polyclonal Antibody

GAPDH Antibody (PA1-988) in WB

Product Details

PA1-988

Western Blot (WB)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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