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Invitrogen
GAPDH Loading Control Polyclonal Antibody, Biotin
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FIGURE: 1 / 1
GAPDH Loading Control Antibody (PA5-142113) in WB
Western Blot analysis of GAPDH in HEK293 lysate (35 µg protein in RIPA buffer) using GAPDH Loading Control Polyclonal Antibody, Biotin (Product # PA5-142113) at 0.5 µg/mL incubated for 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Product Details
PA5-142113
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-HQVVSSDFNSDT (aa 279-290).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:64,000.
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AAG9; aging-associated gene 9 protein; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPD; GAPDH; GAPDH-2; glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12); Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12); Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; OCAS, p38 component; Oct1 coactivator in S phase, 38 Kd component; Peptidyl-cysteine S-nitrosylase GAPDH; similar to Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA with GenBank Accession Number M33197.1; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; unnamed protein product
Gene Aliases: CDABP0047; G3PD; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; OK/SW-cl.12
UniProt ID: (Human) P04406, (Human) O14556, (Mouse) P16858, (Mouse) Q64467
Entrez Gene ID: (Human) 2597, (Human) 26330, (Mouse) 14433, (Mouse) 14447
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
protein binding
microtubule binding
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
transferase activity
aspartic-type endopeptidase inhibitor activity
peptidyl-cysteine S-nitrosylase activity
identical protein binding
NADP binding
NAD binding
disordered domain specific binding
dehydrogenase
microtubule cytoskeleton organization
positive regulation of cytokine production
immune system process
glucose metabolic process
glycolytic process
regulation of translation
apoptotic process
negative regulation of endopeptidase activity
regulation of macroautophagy
negative regulation of translation
killing of cells of other organism
positive regulation of type I interferon production
peptidyl-cysteine S-trans-nitrosylation
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
protein stabilization
defense response to fungus
neuron apoptotic process
killing by host of symbiont cells
antimicrobial humoral immune response mediated by antimicrobial peptide
cellular response to interferon-gamma
sperm motility
positive regulation of glycolytic process
carbohydrate metabolic process
gluconeogenesis
multicellular organism development
oxidation-reduction process
response to ammonium ion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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