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      View (26) other GAPDH antibodies

      Invitrogen

      GAPDH Loading Control Monoclonal Antibody (GA1R)

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      Catalog #:MA5-15738

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      Product Details

      TESTED APPLICATIONS DILUTION
      ELISA (ELISA) Assay Dependent
      Flow Cytometry (Flow) 2 ug/test
      Immunocytochemistry (ICC) 1:20-1:200
      Immunofluorescence (IF) 1:20-1:200
      Immunohistochemistry (Paraffin) (IHC (P)) 1:50-1:500
      Western Blot (WB) 1:1000 - 1:10,000
      PUBLISHED APPLICATIONS
      Western Blot (WB) See 109 publications below
      Miscellaneous PubMed (MISC) See 7 publications below
      Tested Species reactivity Bacteria, Chicken, Hamster, Human, Insect, Mouse, Rabbit, Rat, Yeast
      Published species reactivity Yeast , Rabbit , Rat , Pig , Human , Mouse
      Host / Isotype Mouse / IgG1
      Class Monoclonal
      Type Antibody
      Clone GA1R
      Immunogen Recombinant GAPDH
      Conjugate Unconjugated
      Form Liquid
      Concentration 1 mg/ml
      Purification Protein A
      Storage buffer PBS, pH 7.2
      Contains no preservative
      Storage conditions Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
      RRID AB_10977387

      Product Specific Information

      MA5-15738 detects GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, hamster, and chicken samples.

      MA5-15738 has been successfully used in Western blot, ICC, IF, IHC (P), FACS and ELISA.

      MA5-15738 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

      Background/Target Information

      Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References (116)

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      Bioinformatics

      Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; GAPDH; Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH

      Gene Aliases: BARS-38; CDABP0047; G3PD; GAPD; GAPDH; HEL-S-162eP; I79_001391; KNC-NDS6; OK/SW-cl.12

      UniProt ID: (Chicken) P00356, (Human) P04406, (Rabbit) P46406, (Chinese hamster) P17244

      Entrez Gene ID: (Chicken) 374193, (Human) 2597, (Rabbit) 100009074, (Chinese hamster) 100736557, (Mouse) 14433, (Rat) 24383

      Molecular Function: dehydrogenase oxidoreductase

      Suggested Secondary Antibodies

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