Proteintech

GARS Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

GARS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using GARS antibody 15831-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

GARS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skin cancer using 15831-1-AP (GARS antibody) at dilution of 1:50 (under 40x lens).

GARS Antibody in Western Blot (WB) — Human testis tissue were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

GARS Antibody in Immunoprecipitation (IP) — IP result of anti-GARS (IP:15831-1-AP, 4ug; Detection:15831-1-AP 1:500) with mouse liver tissue lysate 4000ug.

Product Details

15831-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Fruit fly, Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GARS Fusion Protein Ag8322 (390-739 aa encoded by BC007755) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.19 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MRLGDAVEQ GVINNTVLGY FIGRIYLYLT KVGISPDKLR FRQHMENEMA HYACDCWDAE SKTSYGWIEI VGCADRSCYD LSCHARATKV PLVAEKPLKE PKTVNVVQFE PSKGAIGKAY KKDAKLVMEY LAICDECYIT EMEMLLNEKG EFTIETEGKT FQLTKDMINV KRFQKTLYVE EVVPNVIEPS FGLGRIMYTV FEHTFHVREG DEQRTFFSFP AVVAPFKCSV LPLSQNQEFM PFVKELSEAL TRHGVSHKVD DSSGSIGRRY ARTDEIGVAF GVTIDFDTVN KTPHTATLRD RDSMRQIRAE ISELPSIVQD LANGNITWAD VEARYPLFEG QETGKKETIE E (390-739 aa encoded by BC007755)

Target Information

GARS is a glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This protein is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. The protein has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mutations in glycyl-tRNA synthetase impair mitochondrial metabolism in neurons.

Human molecular genetics

Boczonadi V,Meyer K,Gonczarowska-Jorge H,Griffin H,Roos A,Bartsakoulia M,Bansagi B,Ricci G,Palinkas F,Zahedi RP,Bruni F,Kaspar B,Lochmüller H,Boycott KM,Müller JS,Horvath R

Fri Jun 15 00:00:00 EDT 2018

Mutations in glycyl-tRNA synthetase impair mitochondrial metabolism in neurons.

Human molecular genetics

Boczonadi V,Meyer K,Gonczarowska-Jorge H,Griffin H,Roos A,Bartsakoulia M,Bansagi B,Ricci G,Palinkas F,Zahedi RP,Bruni F,Kaspar B,Lochmüller H,Boycott KM,Müller JS,Horvath R

Fri Jun 15 00:00:00 EDT 2018

HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease.

Brain : a journal of neurology

Benoy V,Van Helleputte L,Prior R,d'Ydewalle C,Haeck W,Geens N,Scheveneels W,Schevenels B,Cader MZ,Talbot K,Kozikowski AP,Vanden Berghe P,Van Damme P,Robberecht W,Van Den Bosch L

Thu Mar 01 00:00:00 EST 2018

HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease.

Brain : a journal of neurology

Benoy V,Van Helleputte L,Prior R,d'Ydewalle C,Haeck W,Geens N,Scheveneels W,Schevenels B,Cader MZ,Talbot K,Kozikowski AP,Vanden Berghe P,Van Damme P,Robberecht W,Van Den Bosch L

Thu Mar 01 00:00:00 EST 2018

Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases.

Nature communications

Niehues S,Bussmann J,Steffes G,Erdmann I,Köhrer C,Sun L,Wagner M,Schäfer K,Wang G,Koerdt SN,Stum M,Jaiswal S,RajBhandary UL,Thomas U,Aberle H,Burgess RW,Yang XL,Dieterich D,Storkebaum E

Fri Jul 03 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: 6.1.1.14; AP-4-A synthetase; Ap4A synthetase; Charcot-Marie-Tooth neuropathy 2D; Charcot-Marie-Tooth neuropathy, neuronal type, D; Diadenosine tetraphosphate synthetase; EC 2.1.2.29; GARTF; glycine tRNA ligase; Glycine--tRNA ligase; Glycyl-tRNA synthetase; Glycyl-tRNA synthetase 1; GlyRS; MGC47764; PAIS; PGFT; PRGS; storage granule protein 23

Gene Aliases: CMT2D; DSMAV; GARS; GARS1; Gena201; GENA202; GlyRS; HMN5; Nmf249; RGD1559871; Sgrp23; SMAD1

UniProt ID: (Human) P41250, (Rat) Q5I0G4, (Mouse) Q8VC67

Entrez Gene ID: (Human) 2617, (Rat) 297113, (Mouse) 353172

Molecular Function: aminoacyl-tRNA synthetase translational protein