Invitrogen

GAS1 Polyclonal Antibody

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Questions & Answers

Questions & Answers

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GAS1 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of Gas1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-mouse Gas1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47360) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB and counterstained with hematoxylin (blue). Specific staining was localized to developing brain.

Product Details

PA5-47360

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1 µg/mL	-
Immunohistochemistry (IHC)	5-15 µg/mL	-
ELISA (ELISA)	0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)	-

Product Specifications
Species Reactivity	Mouse
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Gas1 Leu39-Ser315 View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2608167

Product Specific Information

In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human Gas1 and recombinant mouse Gas6 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GAS-1; Growth arrest-specific protein 1

Gene Aliases: AW554192; Gas-1; Gas1

UniProt ID: (Mouse) Q01721

Entrez Gene ID: (Mouse) 14451

positive regulation of mesenchymal cell proliferation cell cycle cell cycle arrest axon guidance positive regulation of cell proliferation regulation of smoothened signaling pathway dorsal/ventral pattern formation negative regulation of protein processing programmed cell death cerebellum morphogenesis dorsal/ventral neural tube patterning negative regulation of cell growth outer ear morphogenesis middle ear morphogenesis odontogenesis embryonic digit morphogenesis camera-type eye development negative regulation of apoptotic process cell fate commitment negative regulation of smoothened signaling pathway positive regulation of smoothened signaling pathway negative regulation of mitotic cell cycle developmental growth eye morphogenesis embryonic morphogenesis embryonic cranial skeleton morphogenesis embryonic skeletal system development positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation palate development regulation of ER to Golgi vesicle-mediated transport negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

Process(es)

protein binding