Invitrogen

GAS6 Polyclonal Antibody

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GAS6 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GAS6 in methanol-fixed MCF-7 cells using a GAS6 polyclonal antibody (Product # PA5-28227) at a 1:500 dilution.

GAS6 Antibody in Western Blot (WB) — Western blot was performed using Anti-GAS6 Polyclonal Antibody (Product # PA5-28227) and a 74-79kDa band corresponding to Growth arrest-specific protein 6 was observed across the cell lines tested. An additional band was also observed at ~50kDa in PANC-1 and MCF7 cell lines, which might correspond to a cleaved C-terminal fragment. Whole-cell extracts (30 µg lysate) of PANC-1 (Lane 1), PANC-1 treated with PTI (1X for 4h) (Lane 2), Hep G2 (Lane 3), Hep G2 treated with PTI (1X for 4h) (Lane 4), U-87 MG (Lane 5), MCF7 (Lane 6) and BJ (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

GAS6 Antibody in Western Blot (WB) — Western blot analysis of GAS6 using A) 30 µg 293T whole cell lysate and B) 30 µg A431 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a GAS6 polyclonal antibody (Product # PA5-28227) at a dilution of 1:1000.

Product Details

PA5-28227

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 283 and 621 of Human GAS6 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.02 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2545703

Product Specific Information

Recommended positive controls: 293T, A431.

Predicted reactivity: Mouse (85%), Rat (86%), Pig (85%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene product is a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AXL receptor tyrosine kinase ligand; AXL stimulatory factor; GAS-6; growth arrest-specific 6; Growth arrest-specific protein 6

Gene Aliases: AXLLG; AXSF; GAS6

UniProt ID: (Human) Q14393

Entrez Gene ID: (Human) 2621

Molecular Function: extracellular matrix protein

neuron migration positive regulation of protein phosphorylation positive regulation of cytokine-mediated signaling pathway platelet degranulation positive regulation of glomerular filtration signal peptide processing protein phosphorylation ER to Golgi vesicle-mediated transport phagocytosis apoptotic process cell cycle arrest cell adhesion signal transduction enzyme linked receptor protein signaling pathway cell proliferation cellular response to starvation positive regulation of gene expression negative regulation of tumor necrosis factor-mediated signaling pathway macrophage cytokine production cell migration peptidyl-serine phosphorylation fusion of virus membrane with host plasma membrane viral genome replication platelet activation organ regeneration cell-substrate adhesion positive regulation of TOR signaling activation of protein kinase B activity negative regulation of interferon-gamma production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of natural killer cell differentiation positive regulation of peptidyl-serine phosphorylation negative regulation of protein import into nucleus, translocation cellular response to interferon-alpha cellular response to drug B cell chemotaxis regulation of growth negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process apoptotic cell clearance negative regulation of sequence-specific DNA binding transcription factor activity protein kinase B signaling positive regulation of protein kinase activity negative regulation of transcription, DNA-templated viral entry into host cell receptor-mediated virion attachment to host cell positive regulation of protein export from nucleus positive regulation of fibroblast proliferation negative regulation of interleukin-1 secretion positive regulation of phagocytosis leukocyte migration positive regulation of protein kinase B signaling positive regulation of protein tyrosine kinase activity negative regulation of biomineral tissue development positive regulation of ERK1 and ERK2 cascade platelet aggregation calcium ion transmembrane transport cellular response to vitamin K cellular response to glucose stimulus cellular response to growth factor stimulus protein targeting to plasma membrane extracellular matrix assembly dendritic cell differentiation hematopoietic stem cell migration to bone marrow negative regulation of oligodendrocyte apoptotic process negative regulation of interleukin-6 secretion negative regulation of fibroblast apoptotic process negative regulation of endothelial cell apoptotic process positive regulation of dendritic cell chemotaxis negative regulation of renal albumin absorption negative regulation of dendritic cell apoptotic process

Process(es)

phosphatidylserine binding receptor binding voltage-gated calcium channel activity calcium ion binding protein binding protein tyrosine kinase activator activity receptor tyrosine kinase binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process receptor agonist activity binding, bridging