Immunofluorescent analysis of GATA4 (green) in embryoid body endoderm generated from Gibco ® Human Episomal iPSC Line grown on Geltrex® in Essential 8TM Medium. After 2 weeks in culture, EB were dissociated with TrypLETM and re-plated onto Geltrex®-coated multi-well plates. Cells were fixed, permeabilized and blocked for immunostaining. Cells were stained with a GATA4 polyclonal antibody (Product # PA1-102) at a dilution of 1:100 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with Alexa Fluor® 488 donkey anti rabbit antibody (Product # A21206) at a 1:500 dilution in conjunction with NucBlue® Fixed Cell Ready Probes® Reagent. After another 3 washes, images were taken on EVOS®Floid® Cell Imaging system at 10X magnification.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Pooled peptides: RKEGIQTRKRKPKNLNKSK and CKQDSWNSLVLADSHGDIITA|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-102 has been successfully used in Western blot, FACS, immunohistochemistry (paraffin) and immunofluorescence applications with human and mouse samples. PA1-102 can be used for immunofluorescence analysis of GATA4 in the endoderm derived from human iPSCs.
GATA binding factor 4 (GATA4) is a GATA family transcription regulator involved in embryonic development. In addition, members of this family recognize the GATA motif which is present in the promoters of many genes. GATA4 is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.