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Invitrogen
GATA4 Recombinant Rat Monoclonal Antibody (eBioEvan), Alexa Fluor™ Plus 555
FIGURE: 1 / 6
GATA4 Antibody (740049TP555) in IHC (P)
Immunohistochemical analysis of GATA-4 was performed using formalin-fixed paraffin-embedded mouse jejunum tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Fo... View More
Product Details
740049TP555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
eBioEvan
Conjugate
Excitation/Emission Max
558/572 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 6.8
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_3679162
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This eBioEvan antibody has been tested by western blot, immunocytochemistry and flow cytometric analysis of Hep G2 cells. This may be used for immunocytochemistry at 5 µg/mL and for flow cytometry at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GAT4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; Transcription factor GATA-4
Gene Aliases: ASD2; Gata-4; GATA4; TACHD; TOF; VSD1
UniProt ID: (Human) P43694, (Mouse) Q08369
Entrez Gene ID: (Human) 2626, (Mouse) 14463
RNA polymerase II regulatory region sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor binding
RNA polymerase II transcription factor binding
enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
transcription factor binding
zinc ion binding
protein kinase binding
activating transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
NFAT protein binding
co-SMAD binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
metal ion binding
DNA-binding transcription factor
heart looping
endocardial cushion development
cardiac ventricle morphogenesis
cardiac right ventricle morphogenesis
ventricular septum development
atrial septum primum morphogenesis
atrial septum secundum morphogenesis
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cell-cell signaling
endoderm development
blood coagulation
male gonad development
response to mechanical stimulus
negative regulation of autophagy
positive regulation of vascular endothelial growth factor production
positive regulation of BMP signaling pathway
response to vitamin A
embryonic heart tube anterior/posterior pattern specification
response to drug
cell fate commitment
positive regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cell development
embryonic foregut morphogenesis
anatomical structure formation involved in morphogenesis
positive regulation of cardioblast differentiation
transdifferentiation
atrial septum morphogenesis
intestinal epithelial cell differentiation
cell growth involved in cardiac muscle cell development
cellular response to glucose stimulus
regulation of cardiac muscle cell contraction
in utero embryonic development
gastrulation with mouth forming second
endoderm formation
heart morphogenesis
outflow tract morphogenesis
atrioventricular valve morphogenesis
atrioventricular valve formation
mitral valve formation
tricuspid valve formation
ventricular cardiac muscle tissue development
cardiac septum development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
spermatogenesis
heart development
regulation of gene expression
cardiac muscle hypertrophy in response to stress
signal transduction involved in regulation of gene expression
BMP signaling pathway
response to retinoic acid
embryonic heart tube development
tube morphogenesis
skeletal muscle cell differentiation
atrioventricular canal development
response to estrogen
embryonic digestive tract morphogenesis
embryonic morphogenesis
cardiac muscle tissue development
cardiac muscle cell differentiation
Sertoli cell differentiation
regulation of cardiac muscle cell proliferation
positive regulation of cardiac muscle cell proliferation
canonical Wnt signaling pathway
SMAD protein signal transduction
lung morphogenesis
lung lobe formation
diaphragm morphogenesis
vasculogenesis involved in coronary vascular morphogenesis
cellular response to gonadotropin stimulus
cellular response to follicle-stimulating hormone stimulus
epithelial cell fate commitment
seminiferous tubule development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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