Western blot analysis of GATA5 was performed by loading 75 µg of HepG2 lysate (Lane 1), 10 µg of HEK293T control (Lane 2), and HEK293T GATA5 overexpression lysate (Lane 3) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing GATA5 (Product # PA1-103) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Pico (Product # 34080).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Pooled peptides RPLVRPQKRLSSSRRA and EDDSLAPGHLEFKFEPED|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-103 has been successfully used in Western blot applications with human samples.
GATA binding factor 5 is a transcription regulator involved in embryonic development. In addition, the protein encoded by this gene contains two GATA-type zinc fingers and is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha). This interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. GATA-5 may be involved in the establishment of cardiac smooth muscle cell diversity.
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