Invitrogen

GBA Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (38) GBA antibodies

Datasheet

Protocols

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Datasheet

Protocols

Tech Support

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GBA Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of GBA was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GBA Polyclonal Antibody (Product # PA5-52319) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Lysosomal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

GBA Antibody in Western Blot (WB) — Western Blot was performed using Anti-GBA Polyclonal Antibody (Product # PA5-52319) and a 60 kDa band corresponding to GBA was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of NIH:OVCAR-3 (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), MCF7 (Lane 4), HEK-293 (Lane 5) and LNCaP (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.2 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

GBA Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. NIH:OVCAR-3 cells were transfected with GBA siRNA and loss of signal was observed in Western Blot application using Anti-GBA Polyclonal Antibody (Product # PA5-52319). {KD}

Product Details

PA5-52319

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	1:20-1:50	-

Product Specifications
Species Reactivity	Human
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human GBA
Conjugate	Unconjugated
Form	Liquid
Concentration	0.11 mg/mL
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2641888

Product Specific Information

Immunogen sequence: YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAA LNILALSPPA QNLLLKSYFS EEGIGYN

Highest antigen sequence identity to the following orthologs: Mouse - 85%, Rat - 90%.

Target Information

GBA encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

In Vivo Remodeling of Altered Autophagy-Lysosomal Pathway by a Phosphopeptide in Lupus.

Cells

Wang F,Tasset I,Cuervo AM,Muller S

PA5-52319 was used in Western Blot to show that this recovery effect of P140 on hyperactivated CMA is not only important for lupus therapy but potentially also for treating other (auto)inflammatory diseases, including neurologic and metabolic disorders, where CMA modulation would be highly beneficial.

Tue Oct 20 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Acid beta-glucosidase; Alglucerase; Beta-GC; Beta-glucocerebrosidase; Beta-glucosylceramidase 1; Cholesterol glucosyltransferase; Cholesteryl-beta-glucosidase; D-glucosyl-N-acylsphingosine glucohydrolase; glucosidase, beta, acid; Glucosylceramidase beta 1; glucosylceramidase-like protein; Imiglucerase; Lysosomal acid GCase; Lysosomal acid glucosylceramidase; Lysosomal cholesterol glycosyltransferase; Lysosomal galactosylceramidase; lysosomal glucocerebrosidase; Lysosomal glycosylceramidase; SGTase

Gene Aliases: GBA; GBA1; GC; GLUC

GBA Polyclonal Antibody

Product Details

PA5-52319

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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