Proteintech

GBP5 Polyclonal Antibody

Product Image Gallery

GBP5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using GBP5 antibody 13220-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

GBP5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer using 13220-1-AP (GBP5 antibody) at dilution of 1:100 (under 40x lens).

GBP5 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 13220-1-AP (GBP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GBP5 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug GBP5 antibody (13220-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

GBP5 Antibody in Immunoprecipitation (IP) — IP result of anti-GBP5 (IP:13220-1-AP, 4ug; Detection:13220-1-AP 1:1000) with U-937 cells lysate 4000 ug.

Product Details

13220-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 10 publications 10 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 4 publications 4 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GBP5 Fusion Protein Ag4103 (382-586 aa encoded by BC031639) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.43 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: LETLLDAKQ NDICKRNLEA SSDYCSALLK DIFGPLEEAV KQGIYSKPGG HNLFIQKTEE LKAKYYREPR KGIQAEEVLQ KYLKSKESVS HAILQTDQAL TETEKKKKEA QVKAEAEKAE AQRLAAIQRQ NEQMMQERER LHQEQVRQME IAKQNWLAEQ QKMQEQQMQE QAAQLSTTFQ AQNRSLLSEL QHAQRTVNND DPCVLL (382-586 aa encoded by BC031639)

Target Information

The guanylate-binding proteins (GBPs) are a family of interferon-induced GTP-binding proteins function in innate immunity against microbial and viral pathogens. In humans, there are seven GBPs from hGBP1 to hGBP7. Human GBP1 and GBP2 have been shown to exhibit antiviral activity as well as being able to regulate the inhibition of proliferation and invasion of endothelial cells in response to interferon (IFN). GBP5 promotes selective NALP3 inflammasome responses to pathogenic bacteria and soluble but not crystalline inflammasome priming agents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

miR-181a-5p Regulates TNF-α and miR-21a-5p Influences Gualynate-Binding Protein 5 and IL-10 Expression in Macrophages Affecting Host Control of Brucella abortus Infection.

Frontiers in immunology

Corsetti PP,de Almeida LA,Gonçalves ANA,Gomes MTR,Guimarães ES,Marques JT,Oliveira SC

Wed Nov 20 00:00:00 EST 2019

Fungal ligands released by innate immune effectors promote inflammasome activation during Aspergillus fumigatus infection.

Nature microbiology

Briard B,Karki R,Malireddi RKS,Bhattacharya A,Place DE,Mavuluri J,Peters JL,Vogel P,Yamamoto M,Kanneganti TD

Fri Feb 01 00:00:00 EST 2019

NADPH Oxidase and Guanylate Binding Protein 5 Restrict Survival of Avirulent Type III Strains of Toxoplasma gondii in Naive Macrophages.

mBio

Matta SK,Patten K,Wang Q,Kim BH,MacMicking JD,Sibley LD

Tue Aug 28 00:00:00 EDT 2018

Expression of PD-L1, indoleamine 2,3-dioxygenase and the immune microenvironment in gastric adenocarcinoma.

Histopathology

Patil PA,Blakely AM,Lombardo KA,Machan JT,Miner TJ,Wang LJ,Marwaha AS,Matoso A

Sun Jul 01 00:00:00 EDT 2018

IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.

Cell

Karki R,Lee E,Place D,Samir P,Mavuluri J,Sharma BR,Balakrishnan A,Malireddi RKS,Geiger R,Zhu Q,Neale G,Kanneganti TD

Thu May 03 00:00:00 EDT 2018

Harnessing the cross-talk between tumor cells and tumor-associated macrophages with a nano-drug for modulation of glioblastoma immune microenvironment.

Journal of controlled release : official journal of the Controlled Release Society

Li TF,Li K,Wang C,Liu X,Wen Y,Xu YH,Zhang Q,Zhao QY,Shao M,Li YZ,Han M,Komatsu N,Zhao L,Chen X

Thu Dec 28 00:00:00 EST 2017

Harnessing the cross-talk between tumor cells and tumor-associated macrophages with a nano-drug for modulation of glioblastoma immune microenvironment.

Journal of controlled release : official journal of the Controlled Release Society

Li TF,Li K,Wang C,Liu X,Wen Y,Xu YH,Zhang Q,Zhao QY,Shao M,Li YZ,Han M,Komatsu N,Zhao L,Chen X

Thu Dec 28 00:00:00 EST 2017

Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.

Nature

Li P,Jiang W,Yu Q,Liu W,Zhou P,Li J,Xu J,Xu B,Wang F,Shao F

Thu Nov 16 00:00:00 EST 2017

Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.

Nature

Li P,Jiang W,Yu Q,Liu W,Zhou P,Li J,Xu J,Xu B,Wang F,Shao F

Thu Nov 16 00:00:00 EST 2017

IFN-γ extends the immune functions of Guanylate Binding Proteins to inflammasome-independent antibacterial activities during Francisella novicida infection.

PLoS pathogens

Wallet P,Benaoudia S,Mosnier A,Lagrange B,Martin A,Lindgren H,Golovliov I,Michal F,Basso P,Djebali S,Provost A,Allatif O,Meunier E,Broz P,Yamamoto M,Py BF,Faudry E,Sjöstedt A,Henry T