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Bethyl Laboratories
GCF Polyclonal Antibody
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FIGURE: 1 / 1
GCF Antibody (A301-919A) in WB
Detection of human and mouse GCF by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-GCF antibody (Product # A301-919A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. GCF was also immunoprecipitated by rabbit anti-GCF antibody A301-920A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A... View More
Product Details
A301-919A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 175 and 225 of human GC-rich sequence DNA-binding factor.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
TCF-9 (transcription factor 9), also known as C2orf3 (chromosome 2 open reading frame 3), GCF (GC-rich sequence DNA-binding factor) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of beta-Actin, EGFR and calcium-dependent protease (CANP) promoters. GCF contains multiple phosphoserine and phosphothreonine residues, and two GCF isoforms are produced due to alternative splicing events. GCF is considered a candidate for susceptibility to dyslexia (DYX3) as both genes reside in close proximity on human chromosome 2. Chromosome 2 is the second largest human chromosome and consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GC binding factor; GC bindng factor; GC-rich sequence DNA-binding factor; GC-rich sequence DNA-binding factor 2; GCF; Intron Large complex component GCFC2; TCF-9; Transcription factor 9; transcription factor 9 (binds GC-rich sequences)
Gene Aliases: A130099G21; AW146020; C2orf3; DNABF; GCF; GCF2; GCFC2; TCF9
UniProt ID: (Human) P16383, (Mouse) Q8BKT3
Entrez Gene ID: (Human) 6936, (Mouse) 330361
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
chromatin/chromatin-binding, or -regulatory protein
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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