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Invitrogen
GCK Polyclonal Antibody
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FIGURE: 1 / 1
GCK Antibody (PA5-90199) in WB
Western blot analysis of GCK in extracts of various cell lines. Samples were incubated with GCK Polyclonal antibody (Product # PA5-90199) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-90199
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
0.55 mg/mL
Amount
55 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: LO2, A-549, mouse liver, rat liver; Cellular Location: Cytoplasm, Nucleus
Target Information
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP:D-hexose 6-phosphotransferase; GC kinase; Glucokinase; glucokinase (hexokinase 4); glucokinase variant 3; hexokinase 4; hexokinase D, pancreatic isozyme; Hexokinase type IV; Hexokinase-4; Hexokinase-D; HK IV; HK4; MEKKK 2; unnamed protein product
Gene Aliases: FGQTL3; GCK; GK; GLK; Gls006; GLUKA; HHF3; HK4; HKIV; Hlb62; HXKP; LGLK; MODY2; PNDM1; RNGK2
UniProt ID: (Human) P35557, (Rat) P17712, (Mouse) P52792
Entrez Gene ID: (Human) 2645, (Rat) 24385, (Mouse) 103988
nucleotide binding
catalytic activity
glucokinase activity
hexokinase activity
protein binding
ATP binding
glucose binding
fructokinase activity
kinase activity
transferase activity
phosphotransferase activity, alcohol group as acceptor
mannokinase activity
glucose sensor activity
magnesium ion binding
protein phosphatase binding
ADP binding
kinase
cellular glucose homeostasis
carbohydrate metabolic process
glucose metabolic process
glucose catabolic process
glycolytic process
regulation of glycolytic process
purine nucleotide metabolic process
NADP metabolic process
response to glucose
hexose metabolic process
positive regulation of insulin secretion
cellular response to insulin stimulus
glucose homeostasis
regulation of potassium ion transport
cellular response to leptin stimulus
negative regulation of gluconeogenesis
positive regulation of glycogen biosynthetic process
nicotinamide nucleotide metabolic process
carbohydrate phosphorylation
regulation of insulin secretion
glucose 6-phosphate metabolic process
canonical glycolysis
calcium ion import
glycogen biosynthetic process
fructose 2,6-bisphosphate metabolic process
positive regulation of cytosolic calcium ion concentration
second-messenger-mediated signaling
negative regulation of epinephrine secretion
cellular response to glucose starvation
positive regulation of phosphorylation
positive regulation of glycolytic process
detection of glucose
lipid homeostasis
phosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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