PA5-16581 targets Gamma-Glutamyl cysteine Synthetase/Glutamate-cysteine Ligase in IP and WB applications and shows reactivity with Bovine, Human, mouse, Non-human primate, and Rat samples.
The PA5-16581 immunogen is a synthetic peptide derived from corresponding to aa 295-313 of rat GCLC (heavy subunit of GCS).
Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS)/Glutamate-cystein Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlle by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: gamma GCS-HS; Gamma-ECS; Gamma-glutamylcysteine synthetase; gamma-glutamylcysteine synthetase heavy subunit; GCS heavy chain; Glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit; Glutamylcysteine gamma synthetase light chain
Gene Aliases: BOS_21297; D9Wsu168e; GCL; GCLC; GCS; Ggcs-hs; GLCL; GLCL-H; GLCLC
Molecular Function: ligase