Western blot analysis of Human Skeletal Muscle Tissue Lysate using PA5-19702, GCLC primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19702 staining of Human Kidney Tissue Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human GCLC.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with cow based on sequence homology.
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cigarette Smoke-Induced Hypermethylation of the GCLC Gene Is Associated With COPD.
PA5-19702 was used in western blot to study the epigenetic regulation of oxidative genes involved in the pathogenesis of chronic obstructive pulmonary disease
|Cheng L,Liu J,Li B,Liu S,Li X,Tu H||Chest (149:474)||2016|
Nuclear Factor, Erythroid 2-Like 2 Regulates Expression of Type 3 Inositol 1,4,5-Trisphosphate Receptor and Calcium Signaling in Cholangiocytes.
PA5-19702 was used in western blot to test if erythroid 2-like 2 regulates expression of ITPR3
|Weerachayaphorn J,Amaya MJ,Spirli C,Chansela P,Mitchell-Richards KA,Ananthanarayanan M,Nathanson MH||Gastroenterology (149:211)||2015|