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Western blot analysis of Human Skeletal Muscle Tissue Lysate using PA5-19702, GCLC primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19702 staining of Human Kidney Tissue Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human , Mouse , Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human GCLC.|
|Conjugate||Unconjugated : Unconjugated : Unconjugated :|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with cow based on sequence homology.
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cigarette Smoke-Induced Hypermethylation of the GCLC Gene Is Associated With COPD.
PA5-19702 was used in western blot to study the epigenetic regulation of oxidative genes involved in the pathogenesis of chronic obstructive pulmonary disease
|Cheng L,Liu J,Li B,Liu S,Li X,Tu H||Chest (149:474)||2016|
gamma GCS-HS; gamma-ECS; gamma-glutamylcysteine synthetase; gamma-glutamylcysteine synthetase heavy subunit; GCL, GCS, GLCL, GLCLC, GCS heavy chain; GCS heavy chain; glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit; Glutamylcysteine gamma synthetase light chain
D9Wsu168e; GCL; GCLC; GCS; Ggcs-hs; GLCL; GLCL-H; GLCLC