Invitrogen

GCLC Polyclonal Antibody

3 References

View all (15) GCLC antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

Antibody Testing Data (4)

GCLC Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

GCLC Antibody (PA5-19702) in ICC/IF

Immunofluorescence analysis GCLC Polyclonal Antibody was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GCLC Polyclonal Antibody (Product # PA5-19702) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More

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GCLC Antibody in Western Blot (WB) — Western blot was performed using Anti-GCLC Polyclonal Antibody (Product # PA5-19702) and a 72 kDa band corresponding to GCLC was observed across cell lines tested along with an uncharacterized band at ~110 kDa. Whole cell extracts (30 µg lysate) of A549 (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), Jurkat (Lane 4), Caco-2 (Lane 5), Mouse Liver (Lane 6), Mouse Heart (Lane 7) and Mouse Kidney (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

PA5-19702

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human GCLC.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.9 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11008589

Product Specific Information

This antibody is predicted to react with cow based on sequence homology.

Target Information

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Histone Methyltransferase G9a Drives Chemotherapy Resistance by Regulating the Glutamate-Cysteine Ligase Catalytic Subunit in Head and Neck Squamous Cell Carcinoma.

Molecular cancer therapeutics

Liu CW,Hua KT,Li KC,Kao HF,Hong RL,Ko JY,Hsiao M,Kuo ML,Tan CT

PA5-19702 was used in Western Blotting to suggest histone methyltransferase G9a as a promising target for overcoming cisplatin resistance in head and neck squamous cell carcinoma.

Sat Jul 01 00:00:00 EDT 2017

Cigarette Smoke-Induced Hypermethylation of the GCLC Gene Is Associated With COPD.

Chest

Cheng L,Liu J,Li B,Liu S,Li X,Tu H

PA5-19702 was used in western blot to study the epigenetic regulation of oxidative genes involved in the pathogenesis of chronic obstructive pulmonary disease

Mon Feb 01 00:00:00 EST 2016

Nuclear Factor, Erythroid 2-Like 2 Regulates Expression of Type 3 Inositol 1,4,5-Trisphosphate Receptor and Calcium Signaling in Cholangiocytes.

Gastroenterology

Weerachayaphorn J,Amaya MJ,Spirli C,Chansela P,Mitchell-Richards KA,Ananthanarayanan M,Nathanson MH

PA5-19702 was used in western blot to test if erythroid 2-like 2 regulates expression of ITPR3

Wed Jul 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: gamma GCS-HS; Gamma-ECS; Gamma-glutamylcysteine synthetase; gamma-glutamylcysteine synthetase heavy subunit; GCS heavy chain; Glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit; Glutamylcysteine gamma synthetase light chain

Gene Aliases: GCLC; GLCL; GLCLC