Invitrogen

GCLC Polyclonal Antibody

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GCLC Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (paraffin-embedded) analysis of human bronchial epithelial tissue using an anti-GCLC polyclonal antibody (Product # PA5-44189).; Secondary Antibody: Donkey anti-Rabbit-Cy3.

GCLC Antibody in Western Blot (WB) — Western blot was performed using Anti-GCLC Polyclonal Antibody (Product # PA5-44189) and a 72 kDa band corresponding to GCLC was observed across all the cell lines and tissue tested. Whole cell extracts (30 µg lysate) of A549 (Lane 1), Hep G2 (Lane 2), tissue extracts of Mouse Liver (Lane 3) and Mouse Kidney (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

PA5-44189

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide directed towards the N-terminal of human GCLC View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2608691

Product Specific Information

Peptide sequence: VLETLQEKGE RTNPNHPTLW RPEYGSYMIE GTPGQPYGGT MSEFNTVEAN

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%

Target Information

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Defective protein repair under methionine sulfoxide A deletion drives autophagy and ARE-dependent gene transcription.

Redox biology

Pennington SM,Klutho PR,Xie L,Broadhurst K,Koval OM,McCormick ML,Spitz DR,Grumbach IM

PA5-44189 was used in Western Blotting to provide the first link between autophagy and activation of Nrf2 in the setting of MsrA deletion.

Fri Jun 01 00:00:00 EDT 2018

Defective protein repair under methionine sulfoxide A deletion drives autophagy and ARE-dependent gene transcription.

Redox biology

Pennington SM,Klutho PR,Xie L,Broadhurst K,Koval OM,McCormick ML,Spitz DR,Grumbach IM

PA5-44189 was used in Western Blotting to provide the first link between autophagy and activation of Nrf2 in the setting of MsrA deletion.

Fri Jun 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: gamma GCS-HS; Gamma-ECS; Gamma-glutamylcysteine synthetase; gamma-glutamylcysteine synthetase heavy subunit; GCS heavy chain; Glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit

Gene Aliases: D9Wsu168e; GCL; GCLC; GCS; Ggcs-hs; GLCL; GLCL-H; GLCLC

UniProt ID: (Human) P48506, (Mouse) P97494

Entrez Gene ID: (Human) 2729, (Mouse) 14629

Molecular Function: ligase