Invitrogen

GCLC Polyclonal Antibody

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GCLC Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (paraffin-embedded) analysis of human bronchial epithelial tissue using an anti-GCLC polyclonal antibody (Product # PA5-44189).; Secondary Antibody: Donkey anti-Rabbit-Cy3.

GCLC Antibody in Western Blot (WB) — Western blot was performed using Anti-GCLC Polyclonal Antibody (Product # PA5-44189) and a 72 kDa band corresponding to GCLC was observed across all the cell lines and tissue tested. Whole cell extracts (30 µg lysate) of A549 (Lane 1), Hep G2 (Lane 2), tissue extracts of Mouse Liver (Lane 3) and Mouse Kidney (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:200

Immunofluorescence (IF)

1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Western Blot (WB)

1:1000

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the N-terminal of human GCLC

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2608691

Product Specific Information

Peptide sequence: VLETLQEKGE RTNPNHPTLW RPEYGSYMIE GTPGQPYGGT MSEFNTVEAN

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%

Target Information

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: catalytic subunit; gamma GCS-HS; Gamma-ECS; Gamma-glutamylcysteine synthetase; gamma-glutamylcysteine synthetase heavy subunit; GCL; GCS heavy chain; GLCL; GLCLC; Glutamate--cysteine ligase catalytic subunit; Glutamate-cysteine ligase; glutamate-cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit; Glutamylcysteine gamma synthetase light chain

Gene Aliases: BOS_21297; cb1049; D9Wsu168e; fe36e11; GCL; GCLC; GCS; Ggcs-hs; GLCL; GLCL-H; GLCLC; wu:fe36e11

UniProt ID: (Human) P48506, (Rat) P19468, (Mouse) P97494

Entrez Gene ID: (Human) 2729, (Rabbit) 100342383, (Dog) 609822, (Bovine) 512468, (Rat) 25283, (Mouse) 14629, (Zebrafish) 326857

Molecular Function: ligase