Invitrogen

GDF15 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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GDF15 Antibody in Western Blot (WB) — Western blot was performed using Anti-GDF15 Polyclonal Antibody (Product # PA5-17066) and a ~34kDa band corresponding to GDF15 was observed across the cell lines tested except Jurkat which is reported to be negative. The expression of protein also increased in HCT 116 upon thapsigargin treatment. Whole cell extracts (30ug lysate) of LNCaP (Lane 1), LNCaP treated with PTI (1X for 4 hr) (Lane2), HCT 116 (Lane 3), HCT 116 treated with PTI (1X for 4hr) (Lane 4), HCT 116 treated with thapsigargin (16uM for 16hr) and PTI (1X for 4 hr) (Lane 5), Jurkat (Lane 6) and Jurkat treated with PTI (1X for 4hr) (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

GDF15 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of GDF15 was observed in LNCaP treated with PTI and HCT116 treated with PTI in comparison to Jurkat treated with PTI using GDF15 Polyclonal Antibody (Product # PA5-17066) in western blot. {RE}

Product Details

PA5-17066

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-

Product Specifications
Species Reactivity	Human, Non-human primate
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponsing to residues surrounding Tyr60 of human Mic-1 View antigen
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains	no preservative
Storage conditions	-20°C
RRID	AB_10981320

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Bone morphogenetic proteins (e. g., BMP5, MIM 112265) are members of the transforming growth factor-beta (see TGFB1, MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GDF-15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; NAG-1; NAG1; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; Placental bone morphogenetic protein; Placental TGF-beta; Prostate differentiation factor; PTGF-beta

Gene Aliases: GDF-15; GDF15; MIC-1; MIC1; NAG-1; PDF; PLAB; PTGFB

UniProt ID: (Human) Q99988

Entrez Gene ID: (Human) 9518

Molecular Function: growth factor