Invitrogen

GDF15 Polyclonal Antibody

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GDF15 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of GDF15 in paraffin embedded human prostate using a GDF15 polyclonal antibody (Product #PA5-18415) at a concentration of 5 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.

GDF15 Antibody in Western Blot (WB) — Western blot analysis of GDF15 by a GDF15 monoclonal antibody (Product # PA5-18415) at a concentration of 0.3 µg/mL. Human Testes (A) and negative control Skin (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

GDF15 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of GDF15 in HeLa cells using a polyclonal antibody (Product #PA5-18415). HeLa cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.

Product Details

PA5-18415

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-0.3 µg/mL	-
Immunohistochemistry (IHC)	5 µg/mL	-
Flow Cytometry (Flow)	10 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide sequence (QKTDTGVSLQTYDD) corresponding to the C-terminus amino acids of GDF15 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_10982195

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

Bone morphogenetic proteins (e. g., BMP5, MIM 112265) are members of the transforming growth factor-beta (see TGFB1, MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GDF-15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; NAG-1; NAG1; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; Placental bone morphogenetic protein; Placental TGF-beta; Prostate differentiation factor; PTGF-beta

Gene Aliases: GDF-15; GDF15; MIC-1; MIC1; NAG-1; PDF; PLAB; PTGFB

UniProt ID: (Human) Q99988

Entrez Gene ID: (Human) 9518

Molecular Function: growth factor