Invitrogen

GDF15 Polyclonal Antibody

Product Image Gallery

GDF15 Antibody in Western Blot (WB) — Western blot of HEK293 overexpressing GDF15 using Product # PA5-18415, mock transfection as a control in first lane.

GDF15 Antibody in Flow Cytometry (FLOW) — Flow cytometric analysis of GDF15 in HeLa cells using a GDF15 monoclonal antibody (Product # PA5-18415) at 10 µg/mL for 1hr, depicted by the blue line. The cells were paraformaldehyde fixed and permeabilized with 0.5% Triton. Primary incubation followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

Assay Dependent

Immunohistochemistry (IHC)

5 µg/mL

peptide-ELISA (pep-ELISA)

1:2000

Western Blot (WB)

0.1-0.3 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (QKTDTGVSLQTYDD) corresponding to the C-terminus amino acids of GDF15

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10982195

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

Bone morphogenetic proteins are members of the transforming growth factor-beta superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: GDF-15; GDF15; growth differentiation factor 15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC1; NAG-1; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PDF; PLAB; Placental bone morphogenetic protein; Placental TGF-beta; Prostate differentiation factor; PTGF-beta; PTGFB

Gene Aliases: GDF-15; GDF15; MIC-1; MIC1; NAG-1; PDF; PLAB; PTGFB

UniProt ID: (Human) Q99988

Entrez Gene ID: (Human) 9518

Molecular Function: growth factor signaling molecule