Western blot analysis was performed on membrane enriched extracts (30 ug lysate) of IMR32 (Lane 1), T98G (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4) and HEK-293 (Lane 5).The blots were probed with GDI1 Rabbit polyclonal Antibody (Product # 71-0300, 2 ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 ug/ml, 1:2500 dilution). A ~55 kDa band corresponding to GDI1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE and reg ; 4-12% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Mouse, Rat, Human|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 20 amino acid synthetic peptide derived from bovine brain Rab Guanine Nucleotide Dissociation Inhibitor (GDI).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-3 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 5 publications below|
GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific mental retardation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
High fat diet modulates Trypanosoma cruzi infection associated myocarditis.
71-0300 was used in western blot to determine the effect of high fat diet on regulating acute T. cruzi infection-induced myocarditis
|Nagajyothi F,Weiss LM,Zhao D,Koba W,Jelicks LA,Cui MH,Factor SM,Scherer PE,Tanowitz HB||PLoS neglected tropical diseases (8:null)||2014|
|Rat||Not Cited||EPI64 protein functions as a physiological GTPase-activating protein for Rab27 protein and regulates amylase release in rat parotid acinar cells.||Imai A,Yoshie S,Ishibashi K,Haga-Tsujimura M,Nashida T,Shimomura H,Fukuda M||The Journal of biological chemistry (286:33854)||2011|
|Mouse||Not Cited||Syntaxin-6 SNARE involvement in secretory and endocytic pathways of cultured pancreatic beta-cells.||Kuliawat R,Kalinina E,Bock J,Fricker L,McGraw TE,Kim SR,Zhong J,Scheller R,Arvan P||Molecular biology of the cell (15:1690)||2004|
|Mouse||Not Cited||Deletion of the mental retardation gene Gdi1 impairs associative memory and alters social behavior in mice.||D'Adamo P,Welzl H,Papadimitriou S,Raffaele di Barletta M,Tiveron C,Tatangelo L,Pozzi L,Chapman PF,Knevett SG,Ramsay MF,Valtorta F,Leoni C,Menegon A,Wolfer DP,Lipp HP,Toniolo D||Human molecular genetics (11:2567)||2002|
|Mouse||Not Cited||Estrogen lowers Alzheimer beta-amyloid generation by stimulating trans-Golgi network vesicle biogenesis.||Greenfield JP,Leung LW,Cai D,Kaasik K,Gross RS,Rodriguez-Boulan E,Greengard P,Xu H||The Journal of biological chemistry (277:12128)||2002|