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GEFT Polyclonal Antibody

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GEFT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

GEFT Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

GEFT Antibody in Immunoprecipitation (IP) — IP result of anti-GEFT-Specific (IP:14839-1-AP, 4ug; Detection:14839-1-AP 1:1500) with mouse brain tissue lysate 8000ug.

Product Details

14839-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 11 publications 11 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Target Information

The Rho family of small GTPases act as molecular switches to control a wide range of cellular processes. Guanine nucleotide exchange factors (GEFs), like GEFT, activate Rho GTPases by accelerating GDP/GTP exchange. The Rho family of small GTPases act as molecular switches to control a wide range of cellular processes. Guanine nucleotide exchange factors (GEFs), like GEFT, activate Rho GTPases by accelerating GDP/GTP exchange (Souchet et al., 2002 ).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RSK2 contributes to myogenic vasoconstriction of resistance arteries by activating smooth muscle myosin and the Na⁺/H⁺ exchanger.

Science signaling

Artamonov MV,Sonkusare SK,Good ME,Momotani K,Eto M,Isakson BE,Le TH,Cope EL,Derewenda ZS,Derewenda U,Somlyo AV

Tue Oct 30 00:00:00 EDT 2018

The blocking of angiotensin II type 1 receptor and RhoA/Rho kinase activity in hypertensive patients: Effect of olmesartan medoxomil and implication with cardiovascular-renal remodeling.

Journal of the renin-angiotensin-aldosterone system : JRAAS

Ravarotto V,Pagnin E,Maiolino G,Fragasso A,Carraro G,Rossi B,Calò LA

Tue Dec 01 00:00:00 EST 2015

p63RhoGEF regulates auto- and paracrine signaling in cardiac fibroblasts.

Journal of molecular and cellular cardiology

Ongherth A,Pasch S,Wuertz CM,Nowak K,Kittana N,Weis CA,Jatho A,Vettel C,Tiburcy M,Toischer K,Hasenfuss G,Zimmermann WH,Wieland T,Lutz S

Sun Nov 01 00:00:00 EDT 2015

p63RhoGEF regulates auto- and paracrine signaling in cardiac fibroblasts.

Journal of molecular and cellular cardiology

Ongherth A,Pasch S,Wuertz CM,Nowak K,Kittana N,Weis CA,Jatho A,Vettel C,Tiburcy M,Toischer K,Hasenfuss G,Zimmermann WH,Wieland T,Lutz S

Sun Nov 01 00:00:00 EDT 2015

Signaling pathways that control rho kinase activity maintain the embryonic epicardial progenitor state.

The Journal of biological chemistry

Artamonov MV,Jin L,Franke AS,Momotani K,Ho R,Dong XR,Majesky MW,Somlyo AV

Fri Apr 17 00:00:00 EDT 2015

Increased level of p63RhoGEF and RhoA/Rho kinase activity in hypertensive patients.

Journal of hypertension

Calò LA,Davis PA,Pagnin E,Dal Maso L,Maiolino G,Seccia TM,Pessina AC,Rossi GP

Sat Feb 01 00:00:00 EST 2014

Agonist-induced Ca2+ sensitization in smooth muscle: redundancy of Rho guanine nucleotide exchange factors (RhoGEFs) and response kinetics, a caged compound study.

The Journal of biological chemistry

Artamonov MV,Momotani K,Stevenson A,Trentham DR,Derewenda U,Derewenda ZS,Read PW,Gutkind JS,Somlyo AV

Fri Nov 22 00:00:00 EST 2013

p63RhoGEF-mediated formation of a single polarized lamellipodium is required for chemotactic migration in breast carcinoma cells.

FEBS letters

Hayashi A,Hiatari R,Tsuji T,Ohashi K,Mizuno K

Mon Mar 18 00:00:00 EDT 2013

p63RhoGEF couples Gα(q/11)-mediated signaling to Ca2+ sensitization of vascular smooth muscle contractility.

Circulation research

Momotani K,Artamonov MV,Utepbergenov D,Derewenda U,Derewenda ZS,Somlyo AV

Fri Oct 14 00:00:00 EDT 2011

TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway.

Genes & development

Kant S,Swat W,Zhang S,Zhang ZY,Neel BG,Flavell RA,Davis RJ

Sat Oct 01 00:00:00 EDT 2011

GEFT, A Rho family guanine nucleotide exchange factor, regulates lens differentiation through a Rac1-mediated mechanism.

Current molecular medicine

Mitchell DC,Bryan BA,Liu L,Hu XH,Huang XQ,Ji WK,Chen PC,Hu WF,Liu JP,Zhang J,Liu M,Li DW

Mon Aug 01 00:00:00 EDT 2011

p63RhoGEF--a key mediator of angiotensin II-dependent signaling and processes in vascular smooth muscle cells.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wuertz CM,Lorincz A,Vettel C,Thomas MA,Wieland T,Lutz S

Wed Dec 01 00:00:00 EST 2010

p63RhoGEF--a key mediator of angiotensin II-dependent signaling and processes in vascular smooth muscle cells.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wuertz CM,Lorincz A,Vettel C,Thomas MA,Wieland T,Lutz S

Wed Dec 01 00:00:00 EST 2010

Modulation of muscle regeneration, myogenesis, and adipogenesis by the Rho family guanine nucleotide exchange factor GEFT.

Molecular and cellular biology

Bryan BA,Mitchell DC,Zhao L,Ma W,Stafford LJ,Teng BB,Liu M

Thu Dec 01 00:00:00 EST 2005

Modulation of muscle regeneration, myogenesis, and adipogenesis by the Rho family guanine nucleotide exchange factor GEFT.

Molecular and cellular biology

Bryan BA,Mitchell DC,Zhao L,Ma W,Stafford LJ,Teng BB,Liu M

Thu Dec 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: Guanine nucleotide exchange factor GEFT; p63RhoGEF; RAC/CDC42 exchange factor; Rac/Cdc42/Rho exchange factor GEFT; Rho guanine nucleotide exchange factor (GEF) 25; Rho guanine nucleotide exchange factor 25; RhoA/RAC/CDC42 exchange factor; RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT

Gene Aliases: 2410008H17Rik; ARHGEF25; D10Ertd610e; GEFT; p63RhoGEF

UniProt ID: (Human) Q86VW2, (Mouse) Q9CWR0, (Rat) Q6P720

Entrez Gene ID: (Human) 115557, (Mouse) 52666, (Rat) 314904

Molecular Function: guanyl-nucleotide exchange factor