Invitrogen

GFAP Polyclonal Antibody

Product Image Gallery

GFAP Antibody — Figure 6 Detection of neuroinflammation after SAH. Sections of ROX SE-perfused brains from mice that underwent sham and SAH surgeries were subjected to fluorescent immunohistochemical staining using anti-alpha-glial acidic fibrillary protein (GFAP) and anti-CD45 antibodies. A marked increase in astrocyte ( A ) and microglial activation ( B ) is observed after SAH. Scale bar: 50 mum.

GFAP Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of 9 days old zebrafish embryo using GFAP polyclonal antibody (Product # OPA1-06100).

Product Details

Tested Applications

Dilution

Immunofluorescence (IF)

1:100-1:200

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

Western Blot (WB)

1:100 - 1:1000

Published Applications

Immunofluorescence (IF)

See 1 publication below

Immunohistochemistry (IHC)

See 1 publication below

Western Blot (WB)

See 1 publication below

Immunocytochemistry (ICC)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Published species

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cytoskeletal preparation of human spinal cord.

Conjugate

Unconjugated

Form

Liquid

Storage buffer

whole serum

Contains

0.09% sodium azide

Storage conditions

4°C or -20°C if preferred

RRID

AB_325657

Product Specific Information

OPA1-06100 detects glial fibrillary acid protein (GFAP) from human, mouse, zebrafish, and rat tissues.

OPA1-06100 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects a single protein representing GFAP rat brain homogenate. Immunohistochemical staining of GFAP in rat brain with OPA1-6100 results in intense staining of astrocytes and Schwann cells.

The OPA1-06100 immunogen is cytoskeletal preparation of human spinal cord.

Store at 4°C or in small aliquots at -20°C.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily, and specifically, expressed in astrocytes and certain astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: FLJ45472; GFAP; Glial fibrillary acidic protein; glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; zGFAP; intermediate filament protein; zrf-1 antigen

Gene Aliases: AI836096; ALXDRD; cb345; etID36982.3; GFAP; gfapl; wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485

UniProt ID: (Human) P14136, (Rat) P47819, (Mouse) P03995, (Zebrafish) Q58EE9

Entrez Gene ID: (Human) 2670, (Rat) 24387, (Mouse) 14580, (Zebrafish) 30646

Molecular Function: cytoskeletal protein intermediate filament structural protein