|Tested species reactivity||Human, Mouse, Rat, Zebrafish|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytoskeletal preparation of human spinal cord.|
|Storage buffer||whole serum|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1:100 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
OPA1-06100 detects glial fibrillary acid protein (GFAP) from human, mouse, zebrafish, and rat tissues.
OPA1-06100 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects a single protein representing GFAP rat brain homogenate. Immunohistochemical staining of GFAP in rat brain with OPA1-6100 results in intense staining of astrocytes and Schwann cells.
The OPA1-06100 immunogen is cytoskeletal preparation of human spinal cord.
Store at 4°C or in small aliquots at -20°C.
Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The nucleotide-binding leucine-rich repeat (NLR) family member NLRX1 mediates protection against experimental autoimmune encephalomyelitis and represses macrophage/microglia-induced inflammation.
OPA1-06100 was used in western blot to study the mechanism by which NLRX1 protects against experimental autoimmune encephalomyelitis
|Eitas TK,Chou WC,Wen H,Gris D,Robbins GR,Brickey J,Oyama Y,Ting JP||The Journal of biological chemistry (289:4173)||2014|
Miz1 is required to maintain autophagic flux.
OPA1-06100 was used in immunohistochemistry to study the role of Miz1 in autophagy
|Wolf E,Gebhardt A,Kawauchi D,Walz S,von Eyss B,Wagner N,Renninger C,Krohne G,Asan E,Roussel MF,Eilers M||Nature communications (4:null)||2013|
Behavioral and neurochemical alterations in mice deficient in anaplastic lymphoma kinase suggest therapeutic potential for psychiatric indications.
OPA1-06100 was used in immunocytochemistry to investigate the role of receptor tyrosine kinase in the hematopoietic and neural systems
|Bilsland JG,Wheeldon A,Mead A,Znamenskiy P,Almond S,Waters KA,Thakur M,Beaumont V,Bonnert TP,Heavens R,Whiting P,McAllister G,Munoz-Sanjuan I||Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (33:685)||2008|