Search
Invitrogen
GFAP Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 7
GFAP Antibody (OPA1-06100) in IHC (F)
Deep tissue imaging with non-curing, refractive index matched SlowFade™ Glass. Cryo-preserved rat brain sections (100 µm thick), stained for tubulin (red) with Mouse Anti-Beta3-Tubulin (Cat. No. MA1-118) and GFAP (yellow) Rabbit Anti-GFAP (Cat. No. OPA1-06100). Targets were detected with Alexa Fluor™ Plus 594 Goat Anti-Mouse (Cat. No. A-11032) and Alexa Fluor™ Plus 647 Goat Anti-Rabbit (Cat. No. A-32733). N... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
OPA1-06100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Published species
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Human spinal cord.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
OPA1-06100 detects glial fibrillary acid protein (GFAP) from human, mouse, zebrafish, and rat tissues.
OPA1-06100 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects a single protein representing GFAP rat brain homogenate. Immunohistochemical staining of GFAP in rat brain with OPA1-6100 results in intense staining of astrocytes and Schwann cells.
The OPA1-06100 immunogen is cytoskeletal preparation of human spinal cord.
Store at 4°C or in small aliquots at -20°C.
Target Information
GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glial fibrillary acidic protein delta; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; unnamed protein product; zGFAP; intermediate filament protein; zrf-1 antigen
Gene Aliases: ALXDRD; cb345; etID36982.3; gfapl; wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485
UniProt ID: (Human) P14136, (Mouse) P03995, (Rat) P47819, (Zebrafish) Q58EE9
Entrez Gene ID: (Human) 2670, (Mouse) 14580, (Rat) 24387, (Zebrafish) 30646
neural crest cell migration
regulation of protein complex assembly
intermediate filament organization
response to ethanol
enteric nervous system development
positive regulation of glial cell proliferation
regulation of chaperone-mediated autophagy
intracellular protein transport
gene expression
positive regulation of Schwann cell proliferation
negative regulation of neuron projection development
astrocyte development
extracellular matrix organization
neuron projection regeneration
intermediate filament-based process
regulation of neurotransmitter uptake
Bergmann glial cell differentiation
long-term synaptic potentiation
D-aspartate import
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support