Mixed neuron-glial cultures stained with rabbit GFAP (red channel) and chicken vimentin CPCA-Vim (green channel). The fibroblastic cells contain only vimentin and so are green, while astrocytes contain either vimentin and GFAP, so appearing golden, or predominantly GFAP, in which case they appear red. Blue is nuclear DNA stain.
|Tested species reactivity||Bovine, Dog, Chicken, Cat, Guinea pig, Human, Mouse, Pig, Rabbit, Rat|
|Published species reactivity||Rat, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Native GFAP purified from bovine Spinal Cord.|
|Storage buffer||whole serum|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000-1:5000|
|Western Blot (WB)||1:50,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is likely to react with most mammals.
Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Involvement of aberrant cyclin-dependent kinase 5/p25 activity in experimental traumatic brain injury.
PA3-16727 was used in immunohistochemistry - paraffin section and western blot to study experimental traumatic brain injury and the role of aberrant Cdk5/p25 activity
|Yousuf MA,Tan C,Torres-Altoro MI,Lu FM,Plautz E,Zhang S,Takahashi M,Hernandez A,Kernie SG,Plattner F,Bibb JA||Journal of neurochemistry (138:317)||2016|
Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia.
PA3-16727 was used in western blot to examine the role of Trx family proteins in neuronal development and recovery after hypoxia/ischemia and reperfusion.
|Romero JI,Hanschmann EM,Gellert M,Eitner S,Holubiec MI,Blanco-Calvo E,Lillig CH,Capani F||Biochimica et biophysica acta (1850:1274)||2015|
Oral administration of the p38¿ MAPK inhibitor, UR13870, inhibits affective pain behavior after spinal cord injury.
PA3-16727 was used in immunohistochemistry - free floating to test the effect of a p38alpha MAPK inhibitor on spinal cord injury in the rat
|Galan-Arriero I,Avila-Martin G,Ferrer-Donato A,Gomez-Soriano J,Bravo-Esteban E,Taylor J||Pain (155:2188)||2014|