Immunofluorescence analysis of GFAP was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GFAP Rabbit Polyclonal Antibody (PA5-16291) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Bovine, Chicken, Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GFAP isolated from cow spinal cord|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16291 targets Glial Fibrillary Acidic Protein in IHC (P) applications and shows reactivity with Bovine, Chicken, Guinea Pig, Hamster, Human, mouse, Non-human primate, Ovine, and Rat samples.
The PA5-16291 immunogen is gFAP isolated from cow spinal cord.
Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes (i.e., glial cells) and ependymal cells of the central nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Chronic treatment with resveratrol, a natural polyphenol found in grapes, alleviates oxidative stress and apoptotic cell death in ovariectomized female rats subjected to chronic cerebral hypoperfusion.
PA5-16291 was used in immunohistochemistry - paraffin section to study the role of resveratrol treatment during oxidative stress and apoptotic cell death caused be cerebral hypoperfusion in rats
|Ozacmak VH,Sayan-Ozacmak H,Barut F||Nutritional neuroscience (19:176)||2016|
Immunopathological changes in the brain of immunosuppressed mice experimentally infected with Toxocara canis.
PA5-16291 was used in immunohistochemistry - paraffin section to study the pathological and immunological changes in the brain of normal and immunosuppressed mice infected with T. canis
|Eid MM,El-Kowrany SI,Othman AA,El Gendy DI,Saied EM||The Korean journal of parasitology (53:51)||2015|
|Not Applicable||Not Cited||
Rac1 plays an essential role in axon growth and guidance and in neuronal survival in the central and peripheral nervous systems.
PA5-16291 was used in immunohistochemistry to study neuronal survival in the peripheral and central nervous system and axon growth due to the role of Rac1
|Hua ZL,Emiliani FE,Nathans J||Neural development (10:null)||2015|
|Not Applicable||Not Cited||
Involvement of plasmalogens in post-natal retinal vascular development.
PA5-16291 was used in immunohistochemistry to determine the role of post-natal retinal vascular development and plasmalogens
|Saab S,Buteau B,Leclère L,Bron AM,Creuzot-Garcher CP,Bretillon L,Acar N||PloS one (9:null)||2014|
Reactive oxygen species induce procalcitonin expression in trigeminal ganglia glia.
PA5-16291 was used in immunohistochemistry to study the induction of trigeminal ganglia glial expression of procalcitonin by ROS
|Raddant AC,Russo AF||Headache (54:472)||2014|
Perinatal inflammation results in decreased oligodendrocyte numbers in adulthood.
PA5-16291 was used in immunohistochemistry to study the decreased numbers of oligodendrocytes in adult mice subjected to neonatal inflammation and hyperoxia
|Graf AE,Haines KM,Pierson CR,Bolon BN,Houston RH,Velten M,Heyob KM,Rogers LK||Life sciences (94:164)||2014|
Efficient generation of human iPSCs by a synthetic self-replicative RNA.
PA5-16291 was used in immunohistochemistry to develop a synthetic self-replicating RNA replicon expressing reprogramming factors that enables the efficient generation of induced pluripotent stem cells
|Yoshioka N,Gros E,Li HR,Kumar S,Deacon DC,Maron C,Muotri AR,Chi NC,Fu XD,Yu BD,Dowdy SF||Cell stem cell (13:246)||2013|
Live imaging of mouse endogenous neural progenitors migrating in response to an induced tumor.
PA5-16291 was used in immunohistochemistry to identify endogenous neural precursor cells migrating towards a brain damage site
|Elvira G,García I,Benito M,Gallo J,Desco M,Penadés S,Garcia-Sanz JA,Silva A||PloS one (7:null)||2012|
Dynamin 2 regulation of integrin endocytosis, but not VEGF signaling, is crucial for developmental angiogenesis.
PA5-16291 was used in immunocytochemistry to study the role of integrin endocytosis in the mechanism underlying dynamin-2-mediated developmental angiogenesis
|Lee MY,Skoura A,Park EJ,Landskroner-Eiger S,Jozsef L,Luciano AK,Murata T,Pasula S,Dong Y,Bouaouina M,Calderwood DA,Ferguson SM,De Camilli P,Sessa WC||Development (Cambridge, England) (141:1465)||2014|
DCPIB, a specific inhibitor of volume-regulated anion channels (VRACs), inhibits astrocyte proliferation and cell cycle progression via G1/S arrest.
PA5-16291 was used in immunocytochemistry to study the blockade of astrocyte proliferation and cell cycle progression by a VRAC inhibitor
|He D,Luo X,Wei W,Xie M,Wang W,Yu Z||Journal of molecular neuroscience : MN (46:249)||2012|