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Invitrogen
GFAP Polyclonal Antibody
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FIGURE: 1 / 26
GFAP Antibody (PA5-16291) in ICC/IF
Immunofluorescence analysis of GFAP was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GFAP Rabbit Polyclonal Antibody (Product # PA5-16291) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pr... View More
Product Details
PA5-16291
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Sheep,
Rat
Published species
Bacteria,
Fish,
Goat,
Human,
Mouse,
Rat,
Reptile,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
GFAP isolated from cow spinal cord
Conjugate
Form
Liquid
Purification
purified
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA5-16291 targets Glial Fibrillary Acidic Protein in IHC (P) applications and shows reactivity with Bovine, Chicken, Guinea Pig, Hamster, Human, mouse, Non-human primate, Ovine, and Rat samples.
The PA5-16291 immunogen is gFAP isolated from cow spinal cord.
Target Information
GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein
Gene Aliases: AI836096; ALXDRD; GFAP; I79_011607
UniProt ID: (Bovine) Q28115, (Human) P14136, (Rat) P47819, (Mouse) P03995
intermediate filament organization
response to wounding
positive regulation of Schwann cell proliferation
negative regulation of neuron projection development
astrocyte development
extracellular matrix organization
neuron projection regeneration
intermediate filament-based process
regulation of neurotransmitter uptake
Bergmann glial cell differentiation
positive regulation of glial cell proliferation
long-term synaptic potentiation
regulation of protein complex assembly
regulation of chaperone-mediated autophagy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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