Immunohistochemical analysis of GFAP in a PFA-perfused cryosection of Human Hypothalamus tissue. Antigen retrieval was performed with citrate buffer pH 6 at 80C for 30min, followed by application of GFAP antibody (Product # PA5-18598) (0.01µg / ml) and HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (DGEVIKESKQEHKD) corresponding to the C-terminus amino acids of GFAP|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||5-10 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||0.01-0.03 µg/ml|
|Western Blot (WB)||0.01-0.03 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with canine and rat based on sequence homology.
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.