Product References
Interstrand crosslinking of homologous repair template DNA enhances gene editing in human cells.
Nature biotechnology
Ghasemi HI,Bacal J,Yoon AC,Tavasoli KU,Cruz C,Vu JT,Gardner BM,Richardson CD
A-11122 was used in Western Blotting to describe a strategy to boost the efficiency of gene editing via homology-directed repair (HDR) by covalently modifying the template DNA with interstrand crosslinks.
Sun Oct 01 00:00:00 EDT 2023
Loss of MBNL1-mediated retrograde BDNF signaling in the myotonic dystrophy brain.
Acta neuropathologica communications
Wang PY,Kuo TY,Wang LH,Liang WH,Wang GS
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation to support that impaired MBNL1-mediated retrograde BDNF-TrkB signaling may contribute to the histopathological phenotypes of DM1.
Wed Mar 15 00:00:00 EDT 2023
LSM1-mediated Major Satellite RNA decay is required for nonequilibrium histone H3.3 incorporation into parental pronuclei.
Nature communications
Zhu J,Chen K,Sun YH,Ye W,Liu J,Zhang D,Su N,Wu L,Kou X,Zhao Y,Wang H,Gao S,Kang L
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot to reveal that accurate histone variant incorporation and incidental modifications in parental pronuclei are specified by LSM1-dependent pericentromeric RNA decay.
Tue Feb 21 00:00:00 EST 2023
TRPV4-Rho GTPase complex structures reveal mechanisms of gating and disease.
Nature communications
Kwon DH,Zhang F,McCray BA,Feng S,Kumar M,Sullivan JM,Im W,Sumner CJ,Lee SY
A-11122 was used in Western Blotting to suggest that RhoA serves as an auxiliary subunit for TRPV4, regulating TRPV4-mediated calcium homeostasis and disruption of TRPV4-RhoA interactions can lead to TRPV4-related neuromuscular disease.
Fri Jun 23 00:00:00 EDT 2023
VEGF Mediates Commissural Axon Chemoattraction through Its Receptor Flk1.
Neuron
Ruiz de Almodovar C,Fabre PJ,Knevels E,Coulon C,Segura I,Haddick PCG,Aerts L,Delattin N,Strasser G,Oh WJ,Lange C,Vinckier S,Haigh J,Fouquet C,Gu C,Alitalo K,Castellani V,Tessier-Lavigne M,Chedotal A,Charron F,Carmeliet P
A-11122 was used in Immunohistochemistry to show that the prototypic angiogenic factor VEGF is secreted by the floor plate and is a chemoattractant for commissural axons in vitro and in vivo.
Wed Apr 19 00:00:00 EDT 2023
Germline protein, Cup, non-cell autonomously limits migratory cell fate in Drosophila oogenesis.
PLoS genetics
Saha B,Acharjee S,Ghosh G,Dasgupta P,Prasad M
A-11122 was used in Immunocytochemistry-immunofluorescence to propose that germline Cup functions through Notch and JAK-STAT to modulate BC fate specification from their static epithelial progenitors.
Wed Feb 01 00:00:00 EST 2023
Leader peptide or pro-segment mutants of renin are misrouted to mitochondria in autosomal dominant tubulointerstitial kidney disease.
Disease models & mechanisms
Schaeffer C,De Fusco M,Pasqualetto E,Scolari C,Izzi C,Scolari F,Rampoldi L
A-11122 was used in Western Blotting to demonstrate a common, unprecedented effect of mutations in the leader peptide and pro-segment as they lead to full or partial mistargeting of the mutated proteins to mitochondria.
Thu Jun 01 00:00:00 EDT 2023
β2 nAChR Activation on VTA DA Neurons Is Sufficient for Nicotine Reinforcement in Rats.
eNeuro
Walker NB,Yan Y,Tapia MA,Tucker BR,Thomas LN,George BE,West AM,Marotta CB,Lester HA,Dougherty DA,Holleran KM,Jones SR,Drenan RM
A-11122 was used in Immunohistochemistry-immunofluorescence to report that β2* nAChR activation on VTA neurons is sufficient for nicotine reinforcement in rats.
Mon May 01 00:00:00 EDT 2023
Rescue of GM3 synthase deficiency by spatially controlled, rAAV-mediated ST3GAL5 delivery.
JCI insight
Yang H,Brown RH,Wang D,Strauss KA,Gao G
A-11122 was used in Immunohistochemistry-immunofluorescence to show that a first-generation rAAV-ST3GAL5 replacement vector using a ubiquitous promoter restored tissue ST3GAL5 expression and normalized cerebral gangliosides in patient-derived induced pluripotent stem cell neurons and brain tissue from St3gal5-KO mice but caused fatal hepatotoxicity when administered systemically.
Mon May 08 00:00:00 EDT 2023
Exosomal release of the virus-encoded chemokine receptor US28 contributes to chemokine scavenging.
iScience
Bebelman MP,Setiawan IM,Bergkamp ND,van Senten JR,Crudden C,Bebelman JPM,Verweij FJ,van Niel G,Siderius M,Pegtel DM,Smit MJ
A-11122 was used in Immunocytochemistry-immunofluorescence to suggest that exosomal release of US28 contributes to chemokine scavenging and immune evasion by human cytomegalovirus.
Fri Aug 18 00:00:00 EDT 2023
Thyroid hormones regulate Zfp423 expression in regionally distinct adipose depots through direct and cell-autonomous action.
Cell reports
Roth L,Johann K,Hönes GS,Oelkrug R,Wagner L,Hoffmann A,Krohn K,Moeller LC,Weiner J,Heiker JT,Klöting N,Tönjes A,Stumvoll M,Blüher M,Mittag J,Krause K
A-11122 was used in Western Blotting to identify Zfp423 as a key molecule that integrates TH signaling into the regulation of adipose tissue plasticity.
Tue Feb 28 00:00:00 EST 2023
A dedicate sensorimotor circuit enables fine texture discrimination by active touch.
PLoS genetics
Yu J,Guo X,Zheng S,Zhang W
A-11122 was used in Immunohistochemistry-immunofluorescence to propose a sensorimotor circuit composed of mechanosensory, gustatory and motor neurons that enables the flies to select ripe yet not over-rotten food by active touch.
Sun Jan 01 00:00:00 EST 2023
Adeno-Associated virus 8 delivers an immunomodulatory peptide to mouse liver more efficiently than to rat liver.
PloS one
Wang Y,Hurley A,De Giorgi M,Tanner MR,Hu RC,Pennington MW,Lagor WR,Beeton C
A-11122 was used in Western Blotting to test the ability of Adeno-Associated Virus (AAV)8 vectors to target hepatocytes for expressing the ShK analog, ShK-235 (AAV-ShK-235) in rodents.
Thu Apr 13 00:00:00 EDT 2023
Altered neuronal activity in the ventromedial prefrontal cortex drives nicotine intake escalation.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
Abarkan M,Fois GR,Vouillac-Mendoza C,Ahmed SH,Guillem K
A-11122 was used in Immunohistochemistry to report that access to unusually high doses of nicotine (i.e., from 30 µg to 240 µg/kg/injection) for self-administration precipitated a rapid and robust escalation of nicotine intake and increased the motivation for the drug in rats.
Mon May 01 00:00:00 EDT 2023
Inducible and Conditional Activation of Adult Neurogenesis Rescues Cadmium-Induced Hippocampus-Dependent Memory Deficits in ApoE4-KI Mice.
International journal of molecular sciences
Matsushita MT,Wang H,Abel GM,Xia Z
A-11122 was used in Immunohistochemistry to provide evidence for a direct link between impaired spatial memory and adult neurogenesis in this GxE model.
Tue May 23 00:00:00 EDT 2023
Synthetic Assembly DNA Cloning to Build Plasmids for Multiplexed Transgenic Selection, Counterselection or Any Other Genetic Strategies Using Drosophila melanogaster.
Current protocols
Venken KJT,Matinyan N,Gonzalez Y,Sarrion-Perdigones A,Dierick HA
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot to demonstrate the functionality of these plasmids by including the expression pattern obtained after co-injection, followed by co-selection using G418 sulfate and blasticidin S, resulting in co-transgenesis of both plasmids.
Wed Feb 01 00:00:00 EST 2023
FMRP, FXR1 protein and Dlg4 mRNA, which are associated with fragile X syndrome, are involved in the ubiquitin-proteasome system.
Scientific reports
Shimizu H,Hohjoh H
A-11122 was used in Western Blotting to provide new insights into the regulatory mechanism of the ubiquitin-proteasome system (UPS) and into the interpretation of the pathogenesis of diseases in which these genes are involved as UPS-related factors.
Thu Feb 02 00:00:00 EST 2023
Negative self-regulation of transient receptor potential canonical 4 by the specific interaction with phospholipase C-δ1.
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology
Ko J,Kim J,Myeong J,Kwak M,So I
A-11122 was used in Western Blotting to demonstrate that TRPC4 self-regulates its activity by allowing Ca2+ ions into the cell and promoting the PIP2 hydrolyzing activity of PLCδ1.
Wed Mar 01 00:00:00 EST 2023
Cocaine-regulated trafficking of dopamine transporters in cultured neurons revealed by a pH sensitive reporter.
iScience
Saenz J,Yao O,Khezerlou E,Aggarwal M,Zhou X,Barker DJ,DiCicco-Bloom E,Pan PY
A-11122 was used in Immunocytochemistry-immunofluorescence to reveal the previously unknown dynamics and molecular regulation for cocaine-regulated DAT trafficking in neuronal processes.
Fri Jan 20 00:00:00 EST 2023
Bipotent transitional liver progenitor cells contribute to liver regeneration.
Nature genetics
Pu W,Zhu H,Zhang M,Pikiolek M,Ercan C,Li J,Huang X,Han X,Zhang Z,Lv Z,Li Y,Liu K,He L,Liu X,Heim MH,Terracciano LM,Tchorz JS,Zhou B
A-11122 was used in Immunohistochemistry to identify a transitional liver progenitor cell (TLPC), which originates from biliary epithelial cells and differentiates into hepatocytes during regeneration from severe liver injury.
Sat Apr 01 00:00:00 EDT 2023
SARS-CoV-2 Nsp1 mediated mRNA degradation requires mRNA interaction with the ribosome.
RNA biology
Shehata SI,Parker R
A-11122 was used in Immunohistochemistry-immunofluorescence to perform mutational analyses of Nsp1, and these revealed that both the N and C terminal domains of Nsp1 are important for translational repression.
Sun Jan 01 00:00:00 EST 2023
Developmental emergence of sleep rhythms enables long-term memory in Drosophila.
Science advances
Poe AR,Zhu L,Szuperak M,McClanahan PD,Anafi RC,Scholl B,Thum AS,Cavanaugh DJ,Kayser MS
A-11122 was used in Immunohistochemistry-immunofluorescence to identify the precise developmental time point when the clock begins to regulate sleep in Drosophila, leading to emergence of sleep rhythms in early third-instars (L3).
Fri Sep 08 00:00:00 EDT 2023
Spatially controlled construction of assembloids using bioprinting.
Nature communications
Roth JG,Brunel LG,Huang MS,Liu Y,Cai B,Sinha S,Yang F,Pașca SP,Shin S,Heilshorn SC
A-11122 was used in Immunohistochemistry to demonstrate the potential for the SPOT platform to construct assembloids which recapitulate key developmental processes and disease etiologies.
Wed Jul 19 00:00:00 EDT 2023
Systematic analysis of the Frazzled receptor interactome establishes previously unreported regulators of axon guidance.
Development (Cambridge, England)
Zang Y,Bashaw GJ
A-11122 was used in Immunohistochemistry-immunofluorescence to identify Fra-interacting proteins in Drosophila.
Tue Aug 01 00:00:00 EDT 2023
Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males.
iScience
Yamanouchi HM,Tanaka R,Kamikouchi A
A-11122 was used in Immunohistochemistry-immunofluorescence to suggest that the mechanosensory system of male genitalia through Piezo channels plays a key role in copulation maintenance and indicate that Piezo may increase male fitness during copulation in flies.
Fri May 19 00:00:00 EDT 2023
An In Vivo Platform for Rebuilding Functional Neocortical Tissue.
Bioengineering (Basel, Switzerland)
Quezada A,Ward C,Bader ER,Zolotavin P,Altun E,Hong S,Killian NJ,Xie C,Batista-Brito R,Hébert JM
A-11122 was used in Immunohistochemistry-immunofluorescence to provide proof of a concept for an in vivo platform that can be used for developing and testing neocortical-like tissue prototypes.
Thu Feb 16 00:00:00 EST 2023
MicroRNA-650 Regulates the Pathogenesis of Alzheimer's Disease Through Targeting Cyclin-Dependent Kinase 5.
Molecular neurobiology
Lin L,Liu X,Cheng X,Li Y,Gearing M,Levey A,Huang X,Li Y,Jin P,Li X
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot to show that miR-650 is able to significantly reduce the expression of APOE, PSEN1, and CDK5 in vitro and significantly alter the CDK5 level and ameliorate AD pathologies in APP-PSEN1 transgenic mice.
Mon May 01 00:00:00 EDT 2023
Role of Nesprin-2 and RanBP2 in BICD2-associated brain developmental disorders.
PLoS genetics
Yi J,Zhao X,Noell CR,Helmer P,Solmaz SR,Vallee RB
A-11122 was used in Western Blotting to propose that mutually exclusive RanBP2-BICD2 vs. Nesprin-2-BICD2 interactions at the NE play successive, critical roles in INM behavior in RGPs and in post-mitotic neuronal migration and errors in these processes contribute to specific human brain malformations.
Wed Mar 01 00:00:00 EST 2023
Differentiation Capacity of Porcine Skeletal Muscle-Derived Stem Cells as Intermediate Species between Mice and Humans.
International journal of molecular sciences
Tamaki T,Natsume T,Katoh A,Nakajima N,Saito K,Fukuzawa T,Otake M,Enya S,Kangawa A,Imai T,Tamaki M,Uchiyama Y
A-11122 was used in Immunohistochemistry to conclude that Sk-34 and Sk-DN cells in pigs are closer to those in humans than to those in mice.
Wed Jun 07 00:00:00 EDT 2023
LC3-associated phagocytosis promotes glial degradation of axon debris after injury in Drosophila models.
Nature communications
Szabó Á,Vincze V,Chhatre AS,Jipa A,Bognár S,Varga KE,Banik P,Harmatos-Ürmösi A,Neukomm LJ,Juhász G
A-11122 was used in Immunohistochemistry-immunofluorescence to reveal a role of glial LAP in the clearance of neuronal debris in vivo, with potential implications for the recovery of the injured nervous system.
Mon May 29 00:00:00 EDT 2023
Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity.
Nature communications
Goretzki B,Wiedemann C,McCray BA,Schäfer SL,Jansen J,Tebbe F,Mitrovic SA,Nöth J,Cabezudo AC,Donohue JK,Jeffries CM,Steinchen W,Stengel F,Sumner CJ,Hummer G,Hellmich UA
A-11122 was used in Western Blotting to demonstrate that IDR structural dynamics are coupled to TRPV4 activity and highlights the importance of IDRs for TRP channel function and regulation.
Thu Jul 13 00:00:00 EDT 2023
Longitudinal visceral muscles in Drosophila fully dedifferentiate and fragment prior to their reestablishment during metamorphosis.
microPublication biology
Schultheis D,Frasch M
A-11122 was used in Immunohistochemistry-immunofluorescence to present our independent analysis using HLH54Fb-eGFP as a cell type specific marker, which strengthens the proposition by Aghajanian et al.
Tue Apr 04 00:00:00 EDT 2023
A Live Cell Protein Complementation Assay for ORFeome-Wide Probing of Human HOX Interactomes.
Cells
Jia Y,Reboulet J,Gillet B,Hughes S,Forcet C,Tribollet V,Hajj Sleiman N,Kundlacz C,Vanacker JM,Bleicher F,Merabet S
A-11122 was used in Western Blotting to demonstrate that the Cell-PCA was pertinent for revealing and, importantly, comparing the interactomes of different or highly related bait proteins in the same cell context.
Tue Jan 03 00:00:00 EST 2023
Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis.
Nature communications
Fankhaenel M,Hashemi FSG,Mourao L,Lucas E,Hosawi MM,Skipp P,Morin X,Scheele CLGJ,Elias S
A-11122 was used in Western Blotting to establish Annexin A1 as an upstream cortical cue that regulates LGN to direct planar cell divisions during mammalian epithelial morphogenesis.
Wed Jan 11 00:00:00 EST 2023
Neural stem cells and oligodendrocyte progenitor cells compete for remyelination in the corpus callosum.
Frontiers in cellular neuroscience
Moyon S,Holloman M,Salzer JL
A-11122 was used in Immunohistochemistry to suggest that pOPCs and NSC-progeny compete to remyelinate the same axons.
Mon Sep 25 00:00:00 EDT 2023
Harnessing the reverse cholesterol transport pathway to favor differentiation of monocyte-derived APCs and antitumor responses.
Cell death & disease
Raccosta L,Marinozzi M,Costantini S,Maggioni D,Ferreira LM,Corna G,Zordan P,Sorice A,Farinello D,Bianchessi S,Riba M,Lazarevic D,Provero P,Mack M,Bondanza A,Nalvarte I,Gustafsson JA,Ranzani V,De Sanctis F,Ugel S,Baron S,Lobaccaro JA,Pontini L,Pacciarini M,Traversari C,Pagani M,Bronte V,Sitia G,Antonson P,Brendolan A,Budillon A,Russo V
A-11122 was used in Immunohistochemistry to support a role for (oxy)sterol metabolism in shaping monocyte-to-DC differentiation, and in tumor antigen presentation critical for responsiveness to immunotherapy.
Wed Feb 15 00:00:00 EST 2023
Sleep/wake changes in perturbational complexity in rats and mice.
iScience
Cavelli ML,Mao R,Findlay G,Driessen K,Bugnon T,Tononi G,Cirelli C
A-11122 was used in Immunohistochemistry to show that PCIst can reliably measure vigilance states in unresponsive animals and support the hypothesis that it is low when an OFF period disrupts causal interactions in cortical networks.
Fri Mar 17 00:00:00 EDT 2023
Endothelial Brg1 fine-tunes Notch signaling during zebrafish heart regeneration.
NPJ Regenerative medicine
Xiao C,Hou J,Wang F,Song Y,Zheng J,Luo L,Wang J,Ding W,Zhu X,Xiong JW
A-11122 was used in Immunohistochemistry-immunofluorescence to demonstrate that the Brg1-Kdm7aa-Notch axis in cardiac endothelial cells, including the endocardium, regulates myocardial proliferation and regeneration via modulating the H3K4me3 of the notch promoters in zebrafish.
Fri Apr 07 00:00:00 EDT 2023
Phosphorylation of Rab29 at Ser185 regulates its localization and role in the lysosomal stress response in concert with LRRK2.
Journal of cell science
Komori T,Kuwahara T,Fujimoto T,Sakurai M,Koyama-Honda I,Fukuda M,Iwatsubo T
A-11122 was used in Immunocytochemistry and Western Blotting to report a novel phosphorylation in Rab29 that is not mediated by LRRK2 and occurs under lysosomal overload stress.
Sat Jul 15 00:00:00 EDT 2023
Phosphorylation of Rab29 at Ser185 regulates its localization and role in the lysosomal stress response in concert with LRRK2.
Journal of cell science
Komori T,Kuwahara T,Fujimoto T,Sakurai M,Koyama-Honda I,Fukuda M,Iwatsubo T
A-11122 was used in Immunocytochemistry and Western Blotting to report a novel phosphorylation in Rab29 that is not mediated by LRRK2 and occurs under lysosomal overload stress.
Sat Jul 15 00:00:00 EDT 2023
Histone bivalency regulates the timing of cerebellar granule cell development.
Genes & development
Mätlik K,Govek EE,Paul MR,Allis CD,Hatten ME
A-11122 was used in Immunohistochemistry to analyze how chromatin modifications control gene expression in a specific type of CNS neuron: the mouse cerebellar granule cell (GC). We found that in proliferating GC progenitors (GCPs), H3K4me3/H3K27me3 bivalency is common at neuronal genes and undergoes dynamic changes that correlate with gene expression during migration and circuit formation.
Sat Jul 01 00:00:00 EDT 2023
Sequential verification of exogenous protein production in OVA gene-targeted chicken bioreactors.
Poultry science
Kim YM,Shim JH,Park JS,Choi HJ,Jung KM,Lee KY,Park KJ,Han JY
A-11122 was used in Immunohistochemistry to evaluate the expression of EGFP protein in the oviduct, along with the physical properties of eggs and egg white from OVALBUMIN (OVA) EGFP knock-in hens.
Sun Jan 01 00:00:00 EST 2023
Region-selective control of the thalamic reticular nucleus via cortical layer 5 pyramidal cells.
Nature neuroscience
Hádinger N,Bősz E,Tóth B,Vantomme G,Lüthi A,Acsády L
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, Immunomicroscopy to show that frontal cortex functions require a highly specialized cortical control over intrathalamic inhibitory processes.
Sun Jan 01 00:00:00 EST 2023
Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control.
Science advances
Schlienger S,Yam PT,Balekoglu N,Ducuing H,Michaud JF,Makihara S,Kramer DK,Chen B,Fasano A,Berardelli A,Hamdan FF,Rouleau GA,Srour M,Charron F
A-11122 was used in Western Blotting to show that Arhgef7 and Git1 are required for Netrin-1-mediated axon guidance and act as a multifunctional effector complex.
Fri May 12 00:00:00 EDT 2023
Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule-actin crosstalk during neurodevelopment.
Proceedings of the National Academy of Sciences of the United States of America
Mun DJ,Goo BS,Suh BK,Hong JH,Woo Y,Kim SJ,Kim S,Lee SB,Won Y,Yoo JY,Cho E,Jang EJ,Nhung TTM,Triet HM,An H,Lee H,Nguyen MD,Park SY,Baek ST,Park SK
A-11122 was used in Immunocytochemistry and Western Blotting to propose that the Gcap14-Ndel1 complex is fundamental for cytoskeletal remodeling during neurodevelopmental processes such as neuronal processes elongation and neuronal migration.
Tue Feb 21 00:00:00 EST 2023
Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule-actin crosstalk during neurodevelopment.
Proceedings of the National Academy of Sciences of the United States of America
Mun DJ,Goo BS,Suh BK,Hong JH,Woo Y,Kim SJ,Kim S,Lee SB,Won Y,Yoo JY,Cho E,Jang EJ,Nhung TTM,Triet HM,An H,Lee H,Nguyen MD,Park SY,Baek ST,Park SK
A-11122 was used in Immunocytochemistry and Western Blotting to propose that the Gcap14-Ndel1 complex is fundamental for cytoskeletal remodeling during neurodevelopmental processes such as neuronal processes elongation and neuronal migration.
Tue Feb 21 00:00:00 EST 2023
Spinal cord repair is modulated by the neurogenic factor Hb-egf under direction of a regeneration-associated enhancer.
Nature communications
Cigliola V,Shoffner A,Lee N,Ou J,Gonzalez TJ,Hoque J,Becker CJ,Han Y,Shen G,Faw TD,Abd-El-Barr MM,Varghese S,Asokan A,Poss KD
A-11122 was used in Immunohistochemistry to identify Hb-egf as a neurogenic factor necessary for innate spinal cord regeneration and suggest strategies to improve spinal cord repair in mammals.
Fri Aug 11 00:00:00 EDT 2023
The Hox transcription factor Ultrabithorax binds RNA and regulates co-transcriptional splicing through an interplay with RNA polymerase II.
Nucleic acids research
Carnesecchi J,Boumpas P,van Nierop Y Sanchez P,Domsch K,Pinto HD,Borges Pinto P,Lohmann I
A-11122 was used in Western Blotting to uncover a novel role of the Hox TFs at the mRNA regulatory layer. This could be an essential function for other classes of TFs to control cell diversity.
Tue Jan 25 00:00:00 EST 2022
A novel reticular node in the brainstem synchronizes neonatal mouse crying with breathing.
Neuron
Wei XP,Collie M,Dempsey B,Fortin G,Yackle K
A-11122 was used in Immunohistochemistry to reveal that a novel mammalian brainstem oscillator embedded within the conserved breathing circuitry plays a central role in the production of neonatal vocalizations.
Wed Feb 16 00:00:00 EST 2022
Valence opponency in peripheral olfactory processing.
Proceedings of the National Academy of Sciences of the United States of America
Wu ST,Chen JY,Martin V,Ng R,Zhang Y,Grover D,Greenspan RJ,Aljadeff J,Su CY
A-11122 was used in Immunohistochemistry to suggest that this organization likely contributes to processing ratio information in odor mixtures.
Tue Feb 01 00:00:00 EST 2022
Paternal transmission of the Wolbachia CidB toxin underlies cytoplasmic incompatibility.
Current biology : CB
Horard B,Terretaz K,Gosselin-Grenet AS,Sobry H,Sicard M,Landmann F,Loppin B
A-11122 was used in Immunohistochemistry to the mechanism by which Wolbachia CI factor genes control the fate of paternal chromosomes at fertilization.
Mon Mar 28 00:00:00 EDT 2022
Regulation of Drosophila courtship behavior by the Tlx/tailless-like nuclear receptor, dissatisfaction.
Current biology : CB
Duckhorn JC,Cande J,Metkus MC,Song H,Altamirano S,Stern DL,Shirangi TR
A-11122 was used in Immunohistochemistry to report the pattern of expression of the dissatisfaction (dsf) gene in the central nervous system and show that the activity of sexually dimorphic abdominal interneurons that co-express dsf and doublesex (dsx) is necessary and sufficient for vaginal plate opening in virgin females, ovipositor extrusion in mated females, and abdominal curling in males during courtship.
Mon Apr 25 00:00:00 EDT 2022
Olfactory stimuli and moonwalker SEZ neurons can drive backward locomotion in Drosophila.
Current biology : CB
Israel S,Rozenfeld E,Weber D,Huetteroth W,Parnas M
A-11122 was used in Immunohistochemistry to reveal an important modality input to MDNs, a novel set of neurons presynaptic to MDNs driving backward locomotion and an MDN-independent backward locomotion pathway.
Mon Mar 14 00:00:00 EDT 2022
Integrated analysis of Wnt signalling system component gene expression.
Development (Cambridge, England)
Murphy P,Armit C,Hill B,Venkataraman S,Frankel P,Baldock RA,Davidson DR
A-11122 was used in Immunocytochemistry-immunoflourescence to report an integrated analysis of key encoding genes in the 3D space of the mouse embryo across multiple stages of development.
Mon Aug 15 00:00:00 EDT 2022
Ankyrin-R Links Kv3.3 to the Spectrin Cytoskeleton and Is Required for Purkinje Neuron Survival.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Stevens SR,van der Heijden ME,Ogawa Y,Lin T,Sillitoe RV,Rasband MN
A-11122 was used in Western Blotting to investigate if Ankyrin scaffolding proteins localize and stabilize ion channels in the membrane by linking them to the spectrin-based cytoskeleton.
Wed Jan 05 00:00:00 EST 2022
Gli1 Regulates the Postnatal Acquisition of Peripheral Nerve Architecture.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Zotter B,Dagan O,Brady J,Baloui H,Samanta J,Salzer JL
A-11122 was used in Immunohistochemistry-immunofluorescence to delineate how peripheral nerves acquire their distinctive organization during normal development, and highlight mechanisms that may regulate their reorganization in pathologic settings, including peripheral neuropathies and nerve injury.
Wed Jan 12 00:00:00 EST 2022
Structure of Long-Range Direct and Indirect Spinocerebellar Pathways as Well as Local Spinal Circuits Mediating Proprioception.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Pop IV,Espinosa F,Blevins CJ,Okafor PC,Ogujiofor OW,Goyal M,Mona B,Landy MA,Dean KM,Gurumurthy CB,Lai HC
A-11122 was used in Western Blotting to define the unique anatomic trajectories of long-range direct and indirect spinocerebellar pathways as well as local intersegmental spinal circuits using genetic tools in both male and female mice.
Wed Jan 26 00:00:00 EST 2022
Pridopidine Promotes Synaptogenesis and Reduces Spatial Memory Deficits in the Alzheimer's Disease APP/PS1 Mouse Model.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics
Estévez-Silva HM,Cuesto G,Romero N,Brito-Armas JM,Acevedo-Arozena A,Acebes Á,Marcellino DJ
A-11122 was used in Immunocytochemistry-immunoflourescence to evaluate the abilities of ACR16 and PRE-084 to induce new synapses and spines formation, analyzing the expression of the possible genes and proteins involved.
Thu Sep 01 00:00:00 EDT 2022
Molecular mechanisms underlying the role of the centriolar CEP164-TTBK2 complex in ciliopathies.
Structure (London, England : 1993)
Rosa E Silva I,Binó L,Johnson CM,Rutherford TJ,Neuhaus D,Andreeva A,Čajánek L,van Breugel M
A-11122 was used in Western Blot to present a detailed biochemical, structural, and functional analysis of the CEP164-TTBK2 complex and demonstrate how it is compromised by two ciliopathic mutations in the centriole distal appendage component CEP164.
Thu Jan 06 00:00:00 EST 2022
Misregulation of Nucleoporins 98 and 96 leads to defects in protein synthesis that promote hallmarks of tumorigenesis.
Disease models & mechanisms
Pulianmackal AJ,Kanakousaki K,Flegel K,Grushko OG,Gourley E,Rozich E,Buttitta LA
A-11122 was used in Immunohistochemistry to suggest that partial loss of Nup98-96 function in translocations could de-regulate protein synthesis, leading to signaling that cooperates with other mutations to promote tumorigenesis.
Tue Mar 01 00:00:00 EST 2022
Bisphenol-A impairs synaptic formation and function by RGS4-mediated regulation of BDNF signaling in the cerebral cortex.
Disease models & mechanisms
Hyun SA,Ko MY,Jang S,Lee BS,Rho J,Kim KK,Kim WY,Ka M
A-11122 was used in Immunohistochemistry to provide molecular mechanistic insights into anatomical and physiological neurotoxic consequences related to a potent endocrine modifier.
Fri Jul 01 00:00:00 EDT 2022
Stage-specific transcription activator ESB1 regulates monoallelic antigen expression in Trypanosoma brucei.
Nature microbiology
López-Escobar L,Hänisch B,Halliday C,Ishii M,Akiyoshi B,Dean S,Sunter JD,Wheeler RJ,Gull K
A-11122 was used in Chromatin immunoprecipitation to show that ESB1 is necessary for recruitment of a subset of ESB components, including RNA polymerase I, revealing that the ESB has separately assembled subdomains.
Mon Aug 01 00:00:00 EDT 2022
Striatonigrostriatal circuit architecture for disinhibition of dopamine signaling.
Cell reports
Ambrosi P,Lerner TN
A-11122 was used in Immunohistochemistry to show that these synapses are unable to modulate tonic dopamine neuron firing, questioning the prominence of their role in mediating crosstalk between striatal subregions.
Tue Aug 16 00:00:00 EDT 2022
A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration.
eLife
Brandão AS,Borbinha J,Pereira T,Brito PH,Lourenço R,Bensimon-Brito A,Jacinto A
A-11122 was used in Immunohistochemistry to indicate that metabolic alterations have a powerful instructive role in regulating genetic programs that dictate fate decisions and stimulate proliferation, thereby providing a deeper understanding on the mechanisms regulating blastema formation and bone regeneration.
Mon Aug 22 00:00:00 EDT 2022
Viral Interference of Hepatitis C and E Virus Replication in Novel Experimental Co-Infection Systems.
Cells
Burkard T,Proske N,Resner K,Collignon L,Knegendorf L,Friesland M,Verhoye L,Sayed IM,Brüggemann Y,Nocke MK,Behrendt P,Wedemeyer H,Meuleman P,Todt D,Steinmann E
A-11122 was used in Immunocytochemistry-immunofluorescence to provide new insights into the pathogenesis of HCV-HEV co-infections and should contribute to its clinical management in the future.
Tue Mar 08 00:00:00 EST 2022
Selectively Imaging Cranial Sensory Ganglion Neurons Using AAV-PHP.S.
eNeuro
Asencor AI,Dvoryanchikov G,Makhoul V,Tsoulfas P,Chaudhari N
A-11122 was used in Immunohistochemistry to suggest that the AAV-PHP.S serotype will be a powerful tool for anatomically and functionally mapping the receptive fields and circuits of the expanding numbers of molecular subtypes of many somatosensory and viscerosensory neurons that continue to be defined via single-cell RNA sequencing.
Wed Jun 08 00:00:00 EDT 2022
Limited conservation in cross-species comparison of GLK transcription factor binding suggested wide-spread cistrome divergence.
Nature communications
Tu X,Ren S,Shen W,Li J,Li Y,Li C,Li Y,Zong Z,Xie W,Grierson D,Fei Z,Giovannoni J,Li P,Zhong S
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, ChIP Assay to show that genome cis-variation caused wide-spread TF binding divergence, and most of the TF binding sites are genetically redundant.
Fri Dec 09 00:00:00 EST 2022
Npas3 regulates stemness maintenance of radial glial cells and neuronal migration in the developing mouse cerebral cortex.
Frontiers in cellular neuroscience
Liu JW,Li H,Zhang Y
A-11122 was used in Western Blotting to demonstrate that Npas3 knockdown profoundly impaired neuronal radial migration and changed the laminar cell fate of the cells detained in the deep cortical layers.
Wed Nov 02 00:00:00 EDT 2022
Npas3 regulates stemness maintenance of radial glial cells and neuronal migration in the developing mouse cerebral cortex.
Frontiers in cellular neuroscience
Liu JW,Li H,Zhang Y
A-11122 was used in Western Blotting to demonstrate that Npas3 knockdown profoundly impaired neuronal radial migration and changed the laminar cell fate of the cells detained in the deep cortical layers.
Wed Nov 02 00:00:00 EDT 2022
Capsaicin-induced depolymerization of axonal microtubules mediates analgesia for trigeminal neuropathic pain.
Pain
Arora V,Li T,Kumari S,Wang S,Asgar J,Chung MK
A-11122 was used in Immunohistochemistry to conclude that further understanding of TRPV1/Ca 2+ -dependent mechanisms of capsaicin-induced ablation and analgesia may help to improve the management of chronic pain.
Mon Aug 01 00:00:00 EDT 2022
Brain motor and fear circuits regulate leukocytes during acute stress.
Nature
Poller WC,Downey J,Mooslechner AA,Khan N,Li L,Chan CT,McAlpine CS,Xu C,Kahles F,He S,Janssen H,Mindur JE,Singh S,Kiss MG,Alonso-Herranz L,Iwamoto Y,Kohler RH,Wong LP,Chetal K,Russo SJ,Sadreyev RI,Weissleder R,Nahrendorf M,Frenette PS,Divangahi M,Swirski FK
A-11122 was used in Immunohistochemistry to show that distinct brain regions differentially and rapidly tailor the leukocyte landscape during psychological stress, thus calibrating the immune system's capacity to respond to physical threats.
Fri Jul 01 00:00:00 EDT 2022
A population of descending neurons that regulates the flight motor of Drosophila.
Current biology : CB
Namiki S,Ros IG,Morrow C,Rowell WJ,Card GM,Korff W,Dickinson MH
A-11122 was used in Immunohistochemistry-immunofluorescence to identify a critical set of descending neurons that provides the sensitivity and dynamic range required for flight control.
Mon Mar 14 00:00:00 EDT 2022
Tsc2 knockout counteracts ubiquitin-proteasome system insufficiency and delays photoreceptor loss in retinitis pigmentosa.
Proceedings of the National Academy of Sciences of the United States of America
Wang Y,Punzo C,Ash JD,Lobanova ES
A-11122 was used in Western Blotting to suggest that chronic activation of the mammalian target of rapamycin complex 1 (mTORC1) pathway in degenerating photoreceptor neurons could stimulate the degradation of ubiquitinated proteins and enhance proteasomal activity through phosphorylation.
Tue Mar 15 00:00:00 EDT 2022
The doublesex gene regulates dimorphic sexual and aggressive behaviors in Drosophila.
Proceedings of the National Academy of Sciences of the United States of America
Han C,Peng Q,Sun M,Jiang X,Su X,Chen J,Ma M,Zhu H,Ji X,Pan Y
A-11122 was used in Immunohistochemistry to reveal crucial roles of dsx, which is broadly conserved from worms to humans, in a small number of neurons in both sexes to establish dimorphic sexual and aggressive behaviors.
Tue Sep 13 00:00:00 EDT 2022
TRPC3 channel gating by lipids requires localization at the ER/PM junctions defined by STIM1.
The Journal of cell biology
Liu H,Lin WY,Leibow SR,Morateck AJ,Ahuja M,Muallem S
A-11122 was used in Western Blotting to identify an FFAT site at the TRPC3 N-terminal loop within the linker helices that envelope the C-terminus pole helix.
Mon May 02 00:00:00 EDT 2022
Pathway-specific inhibition of critical projections from the mediodorsal thalamus to the frontal cortex controls kindled seizures.
Progress in neurobiology
Wicker E,Hyder SK,Forcelli PA
A-11122 was used in Immunohistochemistry to highlight the particular importance of projections from mediodorsal nucleus to prelimbic cortex in the propagation of amygdala-kindled seizures.
Fri Jul 01 00:00:00 EDT 2022
Generation and Comparative Analysis of an Itga8-CreER Mouse with Preferential Activity in Vascular Smooth Muscle Cells.
Nature cardiovascular research
Warthi G,Faulkner JL,Doja J,Ghanam AR,Gao P,Yang AC,Slivano OJ,Barris CT,Kress TC,Zawieja SD,Griffin SH,Xie X,Ashworth A,Christie CK,Bryant WB,Kumar A,Davis MJ,Long X,Gan L,Belin de Chantemele EJ,Lyu Q,Miano JM
A-11122 was used in Western Blotting to present an Itga8-CreER T2 knock-in mouse and compare its activity with a Myh11-CreER T2 mouse.
Tue Nov 01 00:00:00 EDT 2022
Brain-wide electrical dynamics encode individual appetitive social behavior.
Neuron
Mague SD,Talbot A,Blount C,Walder-Christensen KK,Duffney LJ,Adamson E,Bey AL,Ndubuizu N,Thomas GE,Hughes DN,Grossman Y,Hultman R,Sinha S,Fink AM,Gallagher NM,Fisher RL,Jiang YH,Carlson DE,Dzirasa K
A-11122 was used in Immunohistochemistry-immunofluorescence to reveal the architecture whereby the brain integrates distributed activity across timescales to encode an appetitive brain state underlying individual differences in social behavior.
Wed May 18 00:00:00 EDT 2022
GPC3-Unc5 receptor complex structure and role in cell migration.
Cell
Akkermans O,Delloye-Bourgeois C,Peregrina C,Carrasquero-Ordaz M,Kokolaki M,Berbeira-Santana M,Chavent M,Reynaud F,Raj R,Agirre J,Aksu M,White ES,Lowe E,Ben Amar D,Zaballa S,Huo J,Pakos I,McCubbin PTN,Comoletti D,Owens RJ,Robinson CV,Castellani V,Del Toro D,Seiradake E
A-11122 was used in Immunohistochemistry-immunofluorescence to demonstrate a conserved structural mechanism of cell guidance, where finely balanced Unc5-GPC3 interactions regulate cell migration.
Thu Oct 13 00:00:00 EDT 2022
Endophilin-B regulates autophagy during synapse development and neurodegeneration.
Neurobiology of disease
Hernandez-Diaz S,Ghimire S,Sanchez-Mirasierra I,Montecinos-Oliva C,Swerts J,Kuenen S,Verstreken P,Soukup SF
A-11122 was used in Immunohistochemistry to show that EndoB is required to prevent neuronal loss.
Tue Feb 01 00:00:00 EST 2022
Global Proximity Interactome of the Human Macroautophagy Pathway.
Autophagy
Tu YXI,Sydor AM,Coyaud E,Laurent EMN,Dyer D,Mellouk N,St-Germain J,Vernon RM,Forman-Kay JD,Li T,Hua R,Zhao K,Ridgway ND,Kim PK,Raught B,Brumell JH
A-11122 was used in Western Blotting to perform proximity-dependent biotin identification (BioID) on 39 core human macroautophagy proteins, identifying over 700 unique high confidence proximity interactors with new putative connections between macroautophagic and essential cellular processes.
Sun May 01 00:00:00 EDT 2022
The NDNF-like factor Nord is a Hedgehog-induced extracellular BMP modulator that regulates Drosophila wing patterning and growth.
eLife
Yang S,Wu X,Daoutidou EI,Zhang Y,Shimell M,Chuang KH,Peterson AJ,O'Connor MB,Zheng X
A-11122 was used in Immunocytochemistry to propose that Hh-induced Nord expression fine-tunes both the range and strength of BMP signaling in the developing Drosophila wing.
Mon Jan 17 00:00:00 EST 2022
Sex differences in developmental patterns of neocortical astroglia: A mouse translatome database.
Cell reports
Rurak GM,Simard S,Freitas-Andrade M,Lacoste B,Charih F,Van Geel A,Stead J,Woodside B,Green JR,Coppola G,Salmaso N
A-11122 was used in Immunohistochemistry to find sex differences in gene expression patterns across development that peak at P7 and appear to result from males reaching a mature astroglial phenotype earlier than females.
Tue Feb 01 00:00:00 EST 2022
Lateral habenula glutamatergic neurons projecting to the dorsal raphe nucleus promote aggressive arousal in mice.
Nature communications
Takahashi A,Durand-de Cuttoli R,Flanigan ME,Hasegawa E,Tsunematsu T,Aleyasin H,Cherasse Y,Miya K,Okada T,Keino-Masu K,Mitsui K,Li L,Patel V,Blitzer RD,Lazarus M,Tanaka KF,Yamanaka A,Sakurai T,Ogawa S,Russo SJ
A-11122 was used in Immunohistochemistry to demonstrate that the LHb glutamatergic inputs to the DRN promote aggressive arousal induced by social instigation, which contributes to aggressive behavior by activating VTA-projecting non-serotonergic DRN neurons as one of its potential targets.
Thu Jul 21 00:00:00 EDT 2022
Synaptic components are required for glioblastoma progression in Drosophila.
PLoS genetics
Losada-Pérez M,Hernández García-Moreno M,García-Ricote I,Casas-Tintó S
A-11122 was used in Immunohistochemistry to indicate that both presynaptic and postsynaptic proteins play a role in GB progression and lethality.
Fri Jul 01 00:00:00 EDT 2022
A Scribble/Cdep/Rac pathway controls follower-cell crawling and cluster cohesion during collective border-cell migration.
Developmental cell
Campanale JP,Mondo JA,Montell DJ
A-11122 was used in Immunohistochemistry to identify a Rac guanine nucleotide exchange factor, Cdep, which also regulates follower-cell movement and cluster cohesion.
Mon Nov 07 00:00:00 EST 2022
Identification of novel split-GAL4 drivers for the characterization of enteroendocrine cells in the Drosophila melanogaster midgut.
G3 (Bethesda, Md.)
Holsopple JM,Cook KR,Popodi EM
A-11122 was used in Immunohistochemistry-immunofluorescence to identify additional split-GAL4 drivers that promote expression in discrete subpopulations of enteroendocrine cells.
Mon May 30 00:00:00 EDT 2022
Sonic hedgehog-dependent recruitment of GABAergic interneurons into the developing visual thalamus.
eLife
Somaiya RD,Stebbins K,Gingrich EC,Xie H,Campbell JN,Garcia ADR,Fox MA
A-11122 was used in Immunohistochemistry-immunofluorescence to identify a morphogen-dependent neuron-astrocyte signaling mechanism essential for the migration of thalamic interneurons.
Mon Nov 07 00:00:00 EST 2022
Quantitative intracellular retention of delivered RNAs through optimized cell fixation and immunostaining.
RNA (New York, N.Y.)
Paramasivam P,Stöter M,Corradi E,Dalla Costa I,Höijer A,Bartesaghi S,Sabirsh A,Lindfors L,Yanez Arteta M,Nordberg P,Andersson S,Baudet ML,Bickle M,Zerial M
A-11122 was used in Immunohistochemistry-immunofluorescence to optimize cell fixation, permeabilization, and immunostaining of a number of organelle markers, achieving quantitative retention of mRNA and allowing visualization of levels that escape detection using conventional procedures.
Tue Mar 01 00:00:00 EST 2022
Hippocampus as a sorter and reverberatory integrator of sensory inputs.
Nature communications
Nomoto M,Murayama E,Ohno S,Okubo-Suzuki R,Muramatsu SI,Inokuchi K
A-11122 was used in Immunohistochemistry-immunofluorescence to find that synchrony between conditioned stimulus (CS)-, unconditioned stimulus (US)- and future retrieval-responsible cells occurs in the CA1 during the reverberatory phase that emerges after sensory inputs have ceased, but not during CS and US inputs.
Tue Dec 20 00:00:00 EST 2022
RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells.
Science advances
Akturk A,Day M,Tarchini B
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation to suggest that polarized guanine nucleotide exchange factor/GTPase-activating protein junctional activity might dissociate heterotrimeric G proteins, generating free GNAI(GDP) for GPSM2 at the adjacent apical membrane.
Fri Oct 21 00:00:00 EDT 2022
RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells.
Science advances
Akturk A,Day M,Tarchini B
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation to suggest that polarized guanine nucleotide exchange factor/GTPase-activating protein junctional activity might dissociate heterotrimeric G proteins, generating free GNAI(GDP) for GPSM2 at the adjacent apical membrane.
Fri Oct 21 00:00:00 EDT 2022
Reward and aversion processing by input-defined parallel nucleus accumbens circuits in mice.
Nature communications
Zhou K,Xu H,Lu S,Jiang S,Hou G,Deng X,He M,Zhu Y
A-11122 was used in Immunohistochemistry to elucidate the afferent-specific circuit architecture of the NAc in controlling reward and aversion.
Fri Oct 21 00:00:00 EDT 2022
A tumor-suppressive function for Notch3 in the parous mammary gland.
Development (Cambridge, England)
Chung WC,Egan SE,Xu K
A-11122 was used in Immunohistochemistry to report on Notch3 expression and functional analysis in the mouse mammary gland.
Sat Oct 01 00:00:00 EDT 2022
ORP5/8 and MIB/MICOS link ER-mitochondria and intra-mitochondrial contacts for non-vesicular transport of phosphatidylserine.
Cell reports
Monteiro-Cardoso VF,Rochin L,Arora A,Houcine A,Jääskeläinen E,Kivelä AM,Sauvanet C,Le Bars R,Marien E,Dehairs J,Neveu J,El Khallouki N,Santonico E,Swinnen JV,Tareste D,Olkkonen VM,Giordano F
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot to reveal a physical and functional link between ER-mitochondria contacts involved in lipid transfer and intra-mitochondrial membrane contacts maintained by the MIB/MICOS complexes.
Tue Sep 20 00:00:00 EDT 2022
ORP5 and ORP8 orchestrate lipid droplet biogenesis and maintenance at ER-mitochondria contact sites.
The Journal of cell biology
Guyard V,Monteiro-Cardoso VF,Omrane M,Sauvanet C,Houcine A,Boulogne C,Ben Mbarek K,Vitale N,Faklaris O,El Khallouki N,Thiam AR,Giordano F
A-11122 was used in Immunocytochemistry-immunofluorescence to find that ORP5/8 regulates seipin recruitment to these MAM-LD contacts, and their loss impairs LD biogenesis.
Mon Sep 05 00:00:00 EDT 2022
R-type voltage-gated Ca2+ channels mediate A-type K+ current regulation of synaptic input in hippocampal dendrites.
Cell reports
Murphy JG,Gutzmann JJ,Lin L,Hu J,Petralia RS,Wang YX,Hoffman DA
A-11122 was used in Immunocytochemistry to identify a nano-scale interaction between Ca2+ channel subunit Cav2.3 and the pore-forming Kv4.2 subunit that regulates the amplitude of neuronal subthreshold voltage-gated transient K+ current to attenuate spontaneous synaptic input through a Kv4.2 surface localization mechanism.
Tue Jan 18 00:00:00 EST 2022
Confined migration promotes cancer metastasis through resistance to anoikis and increased invasiveness.
eLife
Fanfone D,Wu Z,Mammi J,Berthenet K,Neves D,Weber K,Halaburkova A,Virard F,Bunel F,Jamard C,Hernandez-Vargas H,Tait SWG,Hennino A,Ichim G
A-11122 was used in Western Blot to show in vitro that migrating breast cancer cells develop resistance to anoikis following their passage through microporous membranes mimicking confined migration (CM), a mechanical constriction that cancer cells encounter during metastasis.
Tue Mar 08 00:00:00 EST 2022
The ARSACS disease protein sacsin controls lysosomal positioning and reformation by regulating microtubule dynamics.
The Journal of biological chemistry
Francis V,Alshafie W,Kumar R,Girard M,Brais B,McPherson PS
A-11122 was used in Western Blot, Immunoprecipitation to investigate the molecular details of the function of sacsin.
Thu Sep 01 00:00:00 EDT 2022
The ARSACS disease protein sacsin controls lysosomal positioning and reformation by regulating microtubule dynamics.
The Journal of biological chemistry
Francis V,Alshafie W,Kumar R,Girard M,Brais B,McPherson PS
A-11122 was used in Western Blot, Immunoprecipitation to investigate the molecular details of the function of sacsin.
Thu Sep 01 00:00:00 EDT 2022
NUPR1 protects against hyperPARylation-dependent cell death.
Communications biology
Santofimia-Castaño P,Huang C,Liu X,Xia Y,Audebert S,Camoin L,Peng L,Lomberk G,Urrutia R,Soubeyran P,Neira JL,Iovanna J
A-11122 was used in Western Blotting to conclude that inactivation of NUPR1 induces a hyperPARylation, which in turn, induces a mitochondrial catastrophe and consequently a cell death through a non-canonical Parthanatos, since apoptosis inducing-factor (AIF) is not translocated out of the mitochondria.
Fri Jul 22 00:00:00 EDT 2022
The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots.
Genome biology
Yuan S,Huang T,Bao Z,Wang S,Wu X,Liu J,Liu H,Chen ZJ
A-11122 was used in Immunocytochemistry to provide new insights into how histone modifications and their associated regulatory proteins collectively regulate meiotic homologous recombination.
Tue Sep 06 00:00:00 EDT 2022
The Drosophila microRNA bantam regulates excitability in adult mushroom body output neurons to promote early night sleep.
iScience
Hobin M,Dorfman K,Adel M,Rivera-Rodriguez EJ,Kuklin EA,Ma D,Griffith LC
A-11122 was used in Immunohistochemistry-immunofluorescence to show that bantam acts in the adult to promote early nighttime sleep through a population of glutamatergic neurons that is intimately involved in applying contextual information to behaviors, the γ5β'2a/β'2mp/β'2mp_bilateral Mushroom Body Output Neurons (MBONs).
Fri Sep 16 00:00:00 EDT 2022
Enteroendocrine cell types that drive food reward and aversion.
eLife
Bai L,Sivakumar N,Yu S,Mesgarzadeh S,Ding T,Ly T,Corpuz TV,Grove JCR,Jarvie BC,Knight ZA
A-11122 was used in Immunohistochemistry to establish a cellular basis for how chemosensing in the gut drives learning about food.
Mon Aug 01 00:00:00 EDT 2022
Continuous sensing of IFNα by hepatic endothelial cells shapes a vascular antimetastatic barrier.
eLife
Tran NL,Ferreira LM,Alvarez-Moya B,Buttiglione V,Ferrini B,Zordan P,Monestiroli A,Fagioli C,Bezzecchi E,Scotti GM,Esposito A,Leone R,Gnasso C,Brendolan A,Guidotti LG,Sitia G
A-11122 was used in Immunohistochemistry-immunofluorescence to use mouse models of hepatic metastatization to demonstrate that the continuous infusion of therapeutic doses of interferon-alpha (IFNα) controls CRC invasion by acting on hepatic endothelial cells (HECs).
Tue Oct 25 00:00:00 EDT 2022
Anterior thalamic circuits crucial for working memory.
Proceedings of the National Academy of Sciences of the United States of America
Roy DS,Zhang Y,Aida T,Shen C,Skaggs KM,Hou Y,Fleishman M,Mosto O,Weninger A,Feng G
A-11122 was used in Immunohistochemistry to find essential role of anteroventral (AV) thalamus excitation in maintaing spatial working memory, with fewer unintended effects compared to direct PFC manipulations while resurrecting perfomrance in ageing.
Tue May 17 00:00:00 EDT 2022
Higher-order unimodal olfactory sensory preconditioning in Drosophila.
eLife
Martinez-Cervantes J,Shah P,Phan A,Cervantes-Sandoval I
A-11122 was used in Immunohistochemistry-immunofluorescence to indicate that flies can infer value to olfactory stimuli based on the previous associative structure between odors, and that inhibition of Rac1 lengthens the time window of the olfactory 'sensory buffer', allowing the establishment of associations between odors presented in sequence.
Wed Sep 21 00:00:00 EDT 2022
The consequences of differential origin licensing dynamics in distinct chromatin environments.
Nucleic acids research
Mei L,Kedziora KM,Song EA,Purvis JE,Cook JG
A-11122 was used in Immunocytochemistry-immunofluorescence to show that G1 length is critical for sufficient MCM loading, particularly in heterochromatin, to ensure complete genome duplication and to maintain genome stability.
Fri Sep 23 00:00:00 EDT 2022
Glutamate in primary afferents is required for itch transmission.
Neuron
Cui L,Guo J,Cranfill SL,Gautam M,Bhattarai J,Olson W,Beattie K,Challis RC,Wu Q,Song X,Raabe T,Gradinaru V,Ma M,Liu Q,Luo W
A-11122 was used in Immunohistochemistry to establish a new model in which glutamate is an essential neurotransmitter in primary afferents for itch transmission, whereas neuropeptide neuromedin B (Nmb) signaling enhances its activities.
Wed Mar 02 00:00:00 EST 2022
GABA facilitates spike propagation through branch points of sensory axons in the spinal cord.
Nature neuroscience
Hari K,Lucas-Osma AM,Metz K,Lin S,Pardell N,Roszko DA,Black S,Minarik A,Singla R,Stephens MJ,Pearce RA,Fouad K,Jones KE,Gorassini MA,Fenrich KK,Li Y,Bennett DJ
A-11122 was used in Immunohistochemistry-immunofluorescence to report that activating GAD2+ neurons directly with optogenetics or indirectly by cutaneous stimulation actually facilitates sensory feedback to motor neurons in rodents and humans.
Sat Oct 01 00:00:00 EDT 2022
Nonsynaptic Transmission Mediates Light Context-Dependent Odor Responses in Drosophila melanogaster.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Ikeda K,Kataoka M,Tanaka NK
A-11122 was used in Immunohistochemistry to show that field potential changes in retinae evoked by a sensory stimulus can control the excitability of distant neurons in vivo and mediates multimodal sensory integration in Drosophila melanogaster As such ephaptic transmission is more effective at a short distance, the ephaptic transmission from the retinae may contribute significantly to firing rate changes in downstream neurons of the photoreceptor cells in the optic lobe.
Wed Nov 16 00:00:00 EST 2022
PPM1D mutations are oncogenic drivers of de novo diffuse midline glioma formation.
Nature communications
Khadka P,Reitman ZJ,Lu S,Buchan G,Gionet G,Dubois F,Carvalho DM,Shih J,Zhang S,Greenwald NF,Zack T,Shapira O,Pelton K,Hartley R,Bear H,Georgis Y,Jarmale S,Melanson R,Bonanno K,Schoolcraft K,Miller PG,Condurat AL,Gonzalez EM,Qian K,Morin E,Langhnoja J,Lupien LE,Rendo V,Digiacomo J,Wang D,Zhou K,Kumbhani R,Guerra Garcia ME,Sinai CE,Becker S,Schneider R,Vogelzang J,Krug K,Goodale A,Abid T,Kalani Z,Piccioni F,Beroukhim R,Persky NS,Root DE,Carcaboso AM,Ebert BL,Fuller C,Babur O,Kieran MW,Jones C,Keshishian H,Ligon KL,Carr SA,Phoenix TN,Bandopadhayay P
A-11122 was used in Immunohistochemistry to analyze whole genome sequences of 170 pediatric high-grade gliomas and find that truncating mutations in PPM1D that increase the stability of its phosphatase are clonal driver events in 11% of Diffuse Midline Gliomas and are enriched in primary pontine tumors.
Tue Feb 01 00:00:00 EST 2022
A cortical cell ensemble in the posterior parietal cortex controls past experience-dependent memory updating.
Nature communications
Suzuki A,Kosugi S,Murayama E,Sasakawa E,Ohkawa N,Konno A,Hirai H,Inokuchi K
A-11122 was used in Immunohistochemistry to conclude that a specific subpopulation of PPC cells represents past information and instructs downstream brain regions to update previous memories.
Tue Jan 11 00:00:00 EST 2022
Role of Nrp1 in controlling cortical inter-hemispheric circuits.
eLife
Martín-Fernández F,Bermejo-Santos A,Bragg-Gonzalo L,Briz CG,Serrano-Saiz E,Nieto M
A-11122 was used in Immunohistochemistry to conclude that an exquisite temporal and spatial regulation of Nrp1 expression determines SS interhemispheric maps.
Tue Mar 01 00:00:00 EST 2022
Protocol for analysis of senescent neuronal stem cells in genetic-modified embryonic mice using in utero electroporation technique.
STAR protocols
Chen L,Li Y,Wu Y,Li S,Chang X,Wu H
A-11122 was used in Immunocytochemistry-immunoflourescence to present a detailed protocol for identification and analysis of senescent neuronal stem cells (NSCs) in the developing mouse embryonic neocortex using in utero electroporation.
Fri Sep 16 00:00:00 EDT 2022
Cell-Fibronectin Interactions and Actomyosin Contractility Regulate the Segmentation Clock and Spatio-Temporal Somite Cleft Formation during Chick Embryo Somitogenesis.
Cells
Gomes de Almeida P,Rifes P,Martins-Jesus AP,Pinheiro GG,Andrade RP,Thorsteinsdóttir S
A-11122 was used in Immunohistochemistry to suggest that the fibronectin-integrin-ROCK-NM II axis regulates segmentation clock dynamics and dictates the spatio-temporal localization of somitic clefts.
Wed Jun 22 00:00:00 EDT 2022
The inner junction protein CFAP20 functions in motile and non-motile cilia and is critical for vision.
Nature communications
Chrystal PW,Lambacher NJ,Doucette LP,Bellingham J,Schiff ER,Noel NCL,Li C,Tsiropoulou S,Casey GA,Zhai Y,Nadolski NJ,Majumder MH,Tagoe J,D'Esposito F,Cordeiro MF,Downes S,Clayton-Smith J,Ellingford J,Mahroo OA,Hocking JC,Cheetham ME,Webster AR,Jansen G,Blacque OE,Allison WT,Au PYB,MacDonald IM,Arno G,Leroux MR
A-11122 was used in Immunohistochemistry-immunofluorescence to suggest an uncharacterised pathomechanism for retinal dystrophy, and potentially for motile and non-motile ciliopathies in general.
Thu Nov 03 00:00:00 EDT 2022
AP1/Fra1 confers resistance to MAPK cascade inhibition in pancreatic cancer.
Cellular and molecular life sciences : CMLS
Schneeweis C,Diersch S,Hassan Z,Krauß L,Schneider C,Lucarelli D,Falcomatà C,Steiger K,Öllinger R,Krämer OH,Arlt A,Grade M,Schmidt-Supprian M,Hessmann E,Wirth M,Rad R,Reichert M,Saur D,Schneider G
A-11122 was used in Western Blotting to establish FRA1 as a part of the molecular machinery controlling sensitivity to MAPK cascade inhibition allowing the development of mechanism-based therapies.
Mon Dec 19 00:00:00 EST 2022
The Nab2 RNA-binding protein patterns dendritic and axonal projections through a planar cell polarity-sensitive mechanism.
G3 (Bethesda, Md.)
Corgiat EB,List SM,Rounds JC,Yu D,Chen P,Corbett AH,Moberg KH
A-11122 was used in Immunohistochemistry-immunofluorescence to present an analysis of a brain proteomic dataset that indicates that multiple planar cell polarity proteins are affected by Nab2 loss.
Mon May 30 00:00:00 EDT 2022
Nucleus Accumbens Core Dopamine D2 Receptor-Expressing Neurons Control Reversal Learning but Not Set-Shifting in Behavioral Flexibility in Male Mice.
Frontiers in neuroscience
Macpherson T,Kim JY,Hikida T
A-11122 was used in Immunohistochemistry-immunofluorescence to investigate effects of the nucleus accumbens in mouse brains.
Sat Jul 16 00:00:00 EDT 2022
CRISPR screens in Drosophila cells identify Vsg as a Tc toxin receptor.
Nature
Xu Y,Viswanatha R,Sitsel O,Roderer D,Zhao H,Ashwood C,Voelcker C,Tian S,Raunser S,Perrimon N,Dong M
A-11122 was used in Immunocytochemistry-immunofluorescence to use genome-wide CRISPR-Cas9-mediated knockout screening in Drosophila melanogaster S2R+ cells and identify Visgun (Vsg) as a receptor for an archetypal P.
Sat Oct 01 00:00:00 EDT 2022
Drosophila melanogaster as a model for unraveling unique molecular features of epilepsy elicited by human GABA transporter 1 variants.
Frontiers in neuroscience
Kasture AS,Fischer FP,Kunert L,Burger ML,Burgstaller AC,El-Kasaby A,Hummel T,Sucic S
A-11122 was used in Immunohistochemistry-immunofluorescence to identify three compounds (chemical and pharmacological chaperones) conferring moderate rescue capacity for several variants.
Tue Feb 07 00:00:00 EST 2023
Early developmental, meiosis-specific proteins - Spo11, Msh4-1, and Msh5 - Affect subsequent genome reorganization in Paramecium tetraurelia.
Biochimica et biophysica acta. Molecular cell research
Rzeszutek I,Swart EC,Pabian-Jewuła S,Russo A,Nowacki M
A-11122 was used in Immunocytochemistry-immunofluorescence to understand no coding RNA in Paramecium sexual reproduction.
Wed Jun 01 00:00:00 EDT 2022
Characterization of enhancer fragments in Drosophila robo2.
Fly
Hauptman G,Reichert MC,Abdal Rhida MA,Evans TA
A-11122 was used in Immunocytochemistry-immunofluorescence to provide a general description of each fragment's expression in embryonic tissues outside of the nervous system, to serve as a resource for other researchers interested in robo2 expression and its functional roles outside the central nervous system.
Thu Dec 01 00:00:00 EST 2022
Schizophrenia-associated SAP97 mutations increase glutamatergic synapse strength in the dentate gyrus and impair contextual episodic memory in rats.
Nature communications
Kay Y,Tsan L,Davis EA,Tian C,Décarie-Spain L,Sadybekov A,Pushkin AN,Katritch V,Kanoski SE,Herring BE
A-11122 was used in Western Blot to identify a cell-type-specific synaptic regulatory mechanism in the dentate gyrus that, when disrupted, impairs contextual information processing in rats.
Thu Feb 10 00:00:00 EST 2022
CMTr cap-adjacent 2'-O-ribose mRNA methyltransferases are required for reward learning and mRNA localization to synapses.
Nature communications
Haussmann IU,Wu Y,Nallasivan MP,Archer N,Bodi Z,Hebenstreit D,Waddell S,Fray R,Soller M
A-11122 was used in Immunohistochemistry to show that the two cap methyltransferases of Drosophila can methylate the ribose of the first nucleotide in mRNA.
Tue Mar 08 00:00:00 EST 2022
Calcineurin and huntingtin form a calcium-sensing machinery that directs neurotrophic signals to the nucleus.
Science advances
Scaramuzzino C,Cuoc EC,Pla P,Humbert S,Saudou F
A-11122 was used in Immunohistochemistry to observe similar retrograde waves of TrkA- and epidermal growth factor receptor (EGFR)-bearing endosomes.
Fri Jan 07 00:00:00 EST 2022
Stromal Transcription Factor 21 Regulates Development of the Renal Stroma via Interaction with Wnt/β-Catenin Signaling.
Kidney360
Finer G,Maezawa Y,Ide S,Onay T,Souma T,Scott R,Liang X,Zhao X,Gadhvi G,Winter DR,Quaggin SE,Hayashida T
A-11122 was used in Flow cytometry/Cell sorting to show that Tcf21 in Foxd1+ interstitial progenitors regulates stromal formation and differentiation via interaction with β-catenin.
Thu Jul 28 00:00:00 EDT 2022
Glioblastoma disrupts the ependymal wall and extracellular matrix structures of the subventricular zone.
Fluids and barriers of the CNS
Norton ES,Whaley LA,Ulloa-Navas MJ,García-Tárraga P,Meneses KM,Lara-Velazquez M,Zarco N,Carrano A,Quiñones-Hinojosa A,García-Verdugo JM,Guerrero-Cázares H
A-11122 was used in Immunohistochemistry to investigate the ependymal cell barrier and its derived extracellular matrix (ECM) fractones in the vicinity of a Glioblastoma tumor.
Mon Jul 11 00:00:00 EDT 2022
Potential Variables for Improved Reproducibility of Neuronal Cell Grafts at Stroke Sites.
Cells
Krzyspiak J,Khodakhah K,Hébert JM
A-11122 was used in Immunohistochemistry-immunofluorescence to find that graft size at stroke sites correlates with the size of ischemic damage, host sex (females having graft sizes that correlate with damage), donor cell maturity, and host age, but not with the time of transplant after stroke.
Tue May 17 00:00:00 EDT 2022
Growth cone macropinocytosis of neurotrophin receptor and neuritogenesis are regulated by neuron navigator 1.
Molecular biology of the cell
Powers RM,Daza R,Koehler AE,Courchet J,Calabrese B,Hevner RF,Halpain S
A-11122 was used in Immunocytochemistry-immunofluorescence to demonstrate that Nav1 works at the interface of microtubules, actin, and plasma membrane to organize the cell periphery and promote uptake of growth and guidance cues to facilitate neural morphogenesis during development.
Wed Jun 01 00:00:00 EDT 2022
Single-cell RNA sequencing of motoneurons identifies regulators of synaptic wiring in Drosophila embryos.
Molecular systems biology
Velten J,Gao X,Van Nierop Y Sanchez P,Domsch K,Agarwal R,Bognar L,Paulsen M,Velten L,Lohmann I
A-11122 was used in Immunohistochemistry-immunofluorescence to suggest that homeodomain transcription factor and immunoglobulin molecule expression could be directly linked and function as a crucial determinant of neuronal circuit structure.©2022 The Authors.
Tue Mar 01 00:00:00 EST 2022
Putative COVID-19 therapies imatinib, lopinavir, ritonavir, and ivermectin cause hair cell damage: A targeted screen in the zebrafish lateral line.
Frontiers in cellular neuroscience
Coffin AB,Dale E,Doppenberg E,Fearington F,Hayward T,Hill J,Molano O
A-11122 was used in Immunohistochemistry-immunofluorescence to identify drugs with the fewest side-effects and determine which therapies warrant audiometric monitoring.
Tue Sep 13 00:00:00 EDT 2022
Ubiquitylation by Rab40b/Cul5 regulates Rap2 localization and activity during cell migration.
The Journal of cell biology
Duncan ED,Han KJ,Trout MA,Prekeris R
A-11122 was used in Immunocytochemistry-immunofluorescence to exhibit that Rab40b/Cullin5 ubiquitylates and regulates Rap2-dependent actin dynamics at the leading edge, a process that is required for breast cancer cell migration and invasion.
Mon Apr 04 00:00:00 EDT 2022
The liver contains distinct interconnected networks of CX3CR1+ macrophages, XCR1+ type 1 and CD301a+ type 2 conventional dendritic cells embedded within portal tracts.
Immunology and cell biology
English K,Tan SY,Kwan R,Holz LE,Sierro F,McGuffog C,Kaisho T,Heath WR,MacDonald KP,McCaughan GW,Bowen DG,Bertolino P
A-11122 was used in Immunohistochemistry-immunofluorescence to suggest that portal tracts and their embedded cellular networks of myeloid cells form a distinctive lymphoid compartment in the liver that has the potential to orchestrate immune responses in this organ.
Fri Jul 01 00:00:00 EDT 2022
A nonredundant role for T cell-derived interleukin 22 in antibacterial defense of colonic crypts.
Immunity
Zindl CL,Witte SJ,Laufer VA,Gao M,Yue Z,Janowski KM,Cai B,Frey BF,Silberger DJ,Harbour SN,Singer JR,Turner H,Lund FE,Vallance BA,Rosenberg AF,Schoeb TR,Chen JY,Hatton RD,Weaver CT
A-11122 was used in Immunohistochemistry to demonstrate spatiotemporal differences in the production and action of IL-22 by ILCs and T cells during infection and reveal an indispensable role for IL-22-producing T cells in the protection of the intestinal crypts.
Tue Mar 08 00:00:00 EST 2022
Cytosolic O-GlcNAcylation and PNG1 maintain Drosophila gut homeostasis by regulating proliferation and apoptosis.
PLoS genetics
Na HJ,Abramowitz LK,Hanover JA
A-11122 was used in Immunohistochemistry to reveal a complex coordinated regulation between O-GlcNAcylation and the cytosolic glycanase PNG1 critical to balancing proliferation and apoptosis to maintain gut homeostasis.
Tue Mar 01 00:00:00 EST 2022
CPEB1 directs muscle stem cell activation by reprogramming the translational landscape.
Nature communications
Zeng W,Yue L,Lam KSW,Zhang W,So WK,Tse EHY,Cheung TH
A-11122 was used in Immunoprecipitation to characterize CPEB1 as a key regulator to reprogram the translational landscape directing SC activation and subsequent proliferation.
Thu Feb 17 00:00:00 EST 2022
Cell adhesion molecule KIRREL1 is a feedback regulator of Hippo signaling recruiting SAV1 to cell-cell contact sites.
Nature communications
Paul A,Annunziato S,Lu B,Sun T,Evrova O,Planas-Paz L,Orsini V,Terracciano LM,Charlat O,Loureiro ZY,Ji L,Zamponi R,Sigoillot F,Lei H,Lindeman A,Russ C,Reece-Hoyes JS,Nicholson TB,Tchorz JS,Cong F
A-11122 was used in Immunoprecipitation to suggest that KIRREL1 functions as a feedback regulator of the mammalian Hippo pathway through sensing cell-cell interaction and recruiting SAV1 to cell-cell contact sites.
Thu Feb 17 00:00:00 EST 2022
CaMKIIβ knockdown decreases store-operated calcium entry in hippocampal dendritic spines.
IBRO neuroscience reports
Zernov N,Bezprozvanny I,Popugaeva E
A-11122 was used in Western Blot to show that the effect of CaMKIIβ knockdown on the nSOCE may be associated with a decrease of dendritic spine head size.
Wed Jun 01 00:00:00 EDT 2022
The impact of storage on extracellular vesicles: A systematic study.
Journal of extracellular vesicles
Gelibter S,Marostica G,Mandelli A,Siciliani S,Podini P,Finardi A,Furlan R
A-11122 was used in Western Blotting to show a significant impact of storage on EVs samples in terms of particle loss, purity reduction and fusion phenomena leading to artefactual particles.
Tue Feb 01 00:00:00 EST 2022
SARS-CoV-2 ORF6 disrupts nucleocytoplasmic trafficking to advance viral replication.
Communications biology
Miyamoto Y,Itoh Y,Suzuki T,Tanaka T,Sakai Y,Koido M,Hata C,Wang CX,Otani M,Moriishi K,Tachibana T,Kamatani Y,Yoneda Y,Okamoto T,Oka M
A-11122 was used in Immunocytochemistry-immunoflourescence to conclude that SARS-CoV-2 ORF6 acts as a virulence factor via regulation of nucleocytoplasmic trafficking to accelerate viral replication, resulting in disease progression.
Thu May 19 00:00:00 EDT 2022
Sensory nerves enhance triple-negative breast cancer invasion and metastasis via the axon guidance molecule PlexinB3.
NPJ breast cancer
Le TT,Payne SL,Buckwald MN,Hayes LA,Parker SR,Burge CB,Oudin MJ
A-11122 was used in Immunohistochemistry to identify a mechanism by which nerves contribute to breast cancer migration and metastasis by inducing a shift in TNBC cell gene expression and support the rationale for disrupting neuron-cancer cell interactions to target metastasis.
Fri Nov 04 00:00:00 EDT 2022
Expression of expanded GGC repeats within NOTCH2NLC causes behavioral deficits and neurodegeneration in a mouse model of neuronal intranuclear inclusion disease.
Science advances
Liu Q,Zhang K,Kang Y,Li Y,Deng P,Li Y,Tian Y,Sun Q,Tang Y,Xu K,Zhou Y,Wang JL,Guo J,Li JD,Xia K,Meng Q,Allen EG,Wen Z,Li Z,Jiang H,Shen L,Duan R,Yao B,Tang B,Jin P,Pan Y
A-11122 was used in Immunocytochemistry-immunofluorescence to suggest that dysfunction of hnRNPM could play an important role in the molecular pathogenesis of NIID.
Fri Nov 25 00:00:00 EST 2022
Median raphe serotonergic neurons projecting to the interpeduncular nucleus control preference and aversion.
Nature communications
Kawai H,Bouchekioua Y,Nishitani N,Niitani K,Izumi S,Morishita H,Andoh C,Nagai Y,Koda M,Hagiwara M,Toda K,Shirakawa H,Nagayasu K,Ohmura Y,Kondo M,Kaneda K,Yoshioka M,Kaneko S
A-11122 was used in Immunohistochemistry to reveal an essential function of MRN serotonergic neurons, including 5-HTMRN?IPN, in the processing of reward and aversive stimuli.
Thu Dec 22 00:00:00 EST 2022
Histone H3.3 deposition in seed is essential for the post-embryonic developmental competence in Arabidopsis.
Nature communications
Zhao T,Lu J,Zhang H,Xue M,Pan J,Ma L,Berger F,Jiang D
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, ChIP Assay to suggest a fundamental role of H3.3 in initiating chromatin accessibility at regulatory regions in seed and licensing the embryonic to post-embryonic transition.
Tue Dec 13 00:00:00 EST 2022
A New Transgenic Tool to Study the Ret Signaling Pathway in the Enteric Nervous System.
International journal of molecular sciences
Bandla A,Melancon E,Taylor CR,Davidson AE,Eisen JS,Ganz J
A-11122 was used in Immunohistochemistry-immunofluorescence to find that EPCs and the majority of ENS neurons express ret:GFP during ENS development.
Sat Dec 10 00:00:00 EST 2022
Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA.
Frontiers in neural circuits
Soden ME,Yee JX,Cuevas B,Rastani A,Elum J,Zweifel LS
A-11122 was used in Immunohistochemistry to demonstrate additional diversification of the mesolimbic dopamine system through partially overlapping neuropeptidergic inputs.
Fri Jul 22 00:00:00 EDT 2022
Association of variants in the KIF1A gene with amyotrophic lateral sclerosis.
Translational neurodegeneration
Liao P,Yuan Y,Liu Z,Hou X,Li W,Wen J,Zhang K,Jiao B,Shen L,Jiang H,Guo J,Tang B,Zhang Z,Hu Z,Wang J
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation to determine genetic associations in amyotrophic lateral sclerosis.
Wed Oct 26 00:00:00 EDT 2022
A PKA inhibitor motif within SMOOTHENED controls Hedgehog signal transduction.
Nature structural & molecular biology
Happ JT,Arveseth CD,Bruystens J,Bertinetti D,Nelson IB,Olivieri C,Zhang J,Hedeen DS,Zhu JF,Capener JL,Bröckel JW,Vu L,King CC,Ruiz-Perez VL,Ge X,Veglia G,Herberg FW,Taylor SS,Myers BR
A-11122 was used in Immunoprecipitation to define a mode of GPCR-PKA communication that may be harnessed by a range of membrane receptors and kinases.
Sat Oct 01 00:00:00 EDT 2022
Tight junction formation by a claudin mutant lacking the COOH-terminal PDZ domain-binding motif.
Annals of the New York Academy of Sciences
Fujiwara S,Nguyen TP,Furuse K,Fukazawa Y,Otani T,Furuse M
A-11122 was used in Immunoprecipitation to suggest that the interaction of claudins with ZO proteins is not a prerequisite for TJ formation at the most apical part of cell-cell contacts.
Sat Oct 01 00:00:00 EDT 2022
Traip controls mushroom body size by suppressing mitotic defects.
Development (Cambridge, England)
O'Neill RS,Rusan NM
A-11122 was used in Immunohistochemistry-immunofluorescence to suggest that proper brain size is ensured by the recently described role for TRAIP in unloading stalled replication forks in mitosis, which suppresses DNA bridges and premature neural stem cell loss to promote proper neuron number.
Fri Apr 01 00:00:00 EDT 2022
Consolidation of Sleep-Dependent Appetitive Memory Is Mediated by a Sweet-Sensing Circuit.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Chouhan NS,Sehgal A
A-11122 was used in Immunohistochemistry-immunofluorescence to find that sweet-sensing neurons drive the recruitment of specific reward signaling dopaminergic neurons to establish sleep-dependent memory.
Wed May 04 00:00:00 EDT 2022
An RNA granule for translation quality control in Saccharomyces cerevisiae.
Journal of cell science
Dhaliwal JS,Panozzo C,Benard L,Zerges W
A-11122 was used in Immunocytochemistry-immunofluorescence to show that oxidized RNA bodies are RNA granules in Saccharomyces cerevisiae.
Thu Dec 01 00:00:00 EST 2022
The RNA-binding protein Swm is critical for Drosophila melanogaster intestinal progenitor cell maintenance.
Genetics
Ariyapala IS,Buddika K,Hundley HA,Calvi BR,Sokol NS
A-11122 was used in Immunohistochemistry-immunofluorescence to indicate a nuclear role for Second Mitotic Wave Missing in adult stem cell maintenance, raising the possibility that nuclear post-transcriptional regulation of mRNAs encoding cell adhesion proteins ensures proper attachment of progenitor cells.
Fri Sep 30 00:00:00 EDT 2022
Borna disease virus docks on neuronal DNA double-strand breaks to replicate and dampens neuronal activity.
iScience
Marty FH,Bettamin L,Thouard A,Bourgade K,Allart S,Larrieu G,Malnou CE,Gonzalez-Dunia D,Suberbielle E
A-11122 was used in Western Blot to decipher the interplay between Borna disease virus-1 infection and double-strand breaks, and to unravel its consequences on viral replication and neuronal function.
Fri Jan 21 00:00:00 EST 2022
A descending pathway emanating from the periaqueductal gray mediates the development of cough-like hypersensitivity.
iScience
Chen Z,Lin MT,Zhan C,Zhong NS,Mu D,Lai KF,Liu MJ
A-11122 was used in Immunohistochemistry to show that GABAergic neurons in the lateral/ventrolateral periaqueductal gray suppress cough processing via a descending pathway.
Fri Jan 21 00:00:00 EST 2022
Gpr125 is a unifying hallmark of multiple mammary progenitors coupled to tumor latency.
Nature communications
Spina E,Simundza J,Incassati A,Chandramouli A,Kugler MC,Lin Z,Khodadadi-Jamayran A,Watson CJ,Cowin P
A-11122 was used in Immunohistochemistry to show that high Gpr125 expression identifies patients with particularly poor outcome within the basal breast cancer subtype highlighting its potential utility as a factor to stratify risk.
Thu Mar 17 00:00:00 EDT 2022
Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Nature communications
Xie YX,Naseri NN,Fels J,Kharel P,Na Y,Lane D,Burré J,Sharma M
A-11122 was used in Immunohistochemistry to propose lysosomal exocytosis as a central mechanism for the release of aggregated and degradation-resistant proteins from neurons.
Mon Aug 22 00:00:00 EDT 2022
Deficiency in coatomer complex I causes aberrant activation of STING signalling.
Nature communications
Steiner A,Hrovat-Schaale K,Prigione I,Yu CH,Laohamonthonkul P,Harapas CR,Low RRJ,De Nardo D,Dagley LF,Mlodzianoski MJ,Rogers KL,Zillinger T,Hartmann G,Gantier MP,Gattorno M,Geyer M,Volpi S,Davidson S,Masters SL
A-11122 was used in Western Blotting to suggest targeted inhibition of the cGAS/STING pathway as a promising therapeutic approach.
Thu Apr 28 00:00:00 EDT 2022
The receptor kinase SRF3 coordinates iron-level and flagellin dependent defense and growth responses in plants.
Nature communications
Platre MP,Satbhai SB,Brent L,Gleason MF,Cao M,Grison M,Glavier M,Zhang L,Gaillochet C,Goeschl C,Giovannetti M,Enugutti B,Neveu J,von Reth M,Alcázar R,Parker JE,Vert G,Bayer E,Busch W
A-11122 was used in Immunohistochemistry to report a receptor kinase SRF3, with a role in coordinating root growth, iron homeostasis and immunity pathways via regulation of callose synthases.
Mon Aug 01 00:00:00 EDT 2022
Specificity of the Hox member Deformed is determined by transcription factor levels and binding site affinities.
Nature communications
Pinto PB,Domsch K,Gao X,Wölk M,Carnesecchi J,Lohmann I
A-11122 was used in Immunohistochemistry to show that Hox-regulated morphogenesis seems to result from the co-evolution of Hox binding affinity and Hox dosage for precise target gene regulation.
Fri Aug 26 00:00:00 EDT 2022
Local translation provides the asymmetric distribution of CaMKII required for associative memory formation.
Current biology : CB
Chen N,Zhang Y,Adel M,Kuklin EA,Reed ML,Mardovin JD,Bakthavachalu B,VijayRaghavan K,Ramaswami M,Griffith LC
A-11122 was used in Immunohistochemistry to identify elevated levels of translation of an evenly distributed mRNA as a novel strategy for generating subcellular biochemical asymmetries.
Mon Jun 20 00:00:00 EDT 2022
Oral and Injected Tamoxifen Alter Adult Hippocampal Neurogenesis in Female and Male Mice.
eNeuro
Smith BM,Saulsbery AI,Sarchet P,Devasthali N,Einstein D,Kirby ED
A-11122 was used in Immunohistochemistry-immunofluorescence to provide guidance for selection of TAM administration route and appropriate controls in adult neurogenesis studies using TAM-inducible Cre mice.
Fri Apr 22 00:00:00 EDT 2022
c-Abl-induced Olig2 phosphorylation regulates the proliferation of oligodendrocyte precursor cells.
Glia
Zhang J,Sun JG,Xing X,Wu R,Zhou L,Zhang Y,Yuan F,Wang S,Yuan Z
A-11122 was used in Western Blotting to identify the important role of c-Abl in OLs during developmental myelination and remyelination in a disease model.
Wed Jun 01 00:00:00 EDT 2022
Coordinated repression of pro-differentiation genes via P-bodies and transcription maintains Drosophila intestinal stem cell identity.
Current biology : CB
Buddika K,Huang YT,Ariyapala IS,Butrum-Griffith A,Norrell SA,O'Connor AM,Patel VK,Rector SA,Slovan M,Sokolowski M,Kato Y,Nakamura A,Sokol NS
A-11122 was used in Immunohistochemistry-immunofluorescence to identify a P-body-dependent repression activity that coordinates with known transcriptional repression programs to maintain a population of in vivo stem cells in a state primed for differentiation.
Mon Jan 24 00:00:00 EST 2022
Gene therapy restores adipose tissue and metabolic health in a pre-clinical mouse model of lipodystrophy.
Molecular therapy. Methods & clinical development
Sommer N,Roumane A,Han W,Delibegović M,Rochford JJ,Mcilroy GD
A-11122 was used in Immunohistochemistry to propose that gene therapy offers great potential as a therapeutic strategy to correct multiple metabolic complications in patients with congenital lipodystrophy.
Thu Dec 08 00:00:00 EST 2022
Drosophila gustatory projections are segregated by taste modality and connectivity.
eLife
Engert S,Sterne GR,Bock DD,Scott K
A-11122 was used in Immunohistochemistry-immunofluorescence to resolve the anatomy of labellar gustatory projections, reveals that gustatory projections are segregated based on taste modality, and uncovers synaptic connections that may alter the transmission of gustatory signals.
Wed May 25 00:00:00 EDT 2022
A balance of noncanonical Semaphorin signaling from the cerebrospinal fluid regulates apical cell dynamics during corticogenesis.
Science advances
Gerstmann K,Kindbeiter K,Telley L,Bozon M,Reynaud F,Théoulle E,Charoy C,Jabaudon D,Moret F,Castellani V
A-11122 was used in Immunohistochemistry-immunofluorescence to discover that two different complexes, Sema3B/Nrp2 and Sema3F/Nrp1, exert dual effects on apical endfeet dynamics, nuclei positioning, and RGC progeny.
Wed Nov 16 00:00:00 EST 2022
The coilin N-terminus mediates multivalent interactions between coilin and Nopp140 to form and maintain Cajal bodies.
Nature communications
Courchaine E,Gelles-Watnick S,Machyna M,Straube K,Sauyet S,Enright J,Neugebauer KM
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation to propose a model in which coilin NTD-NTD mediated assemblies make multivalent contacts with Nopp140 to achieve biomolecular condensation in the nucleus.
Wed Oct 12 00:00:00 EDT 2022
Differentiation of Human Adipose-Derived Mesenchymal Stromal/Stem Cells into Insulin-Producing Cells with A Single Tet-Off Lentiviral Vector System.
Cell journal
Moriyama H,Moriyama M,Ozawa T,Tsuruta D,Hayakawa T
A-11122 was used in Western Blotting to investigate whether MafA and NeuroD1 would cooperate with Pdx-1 in the differentiation of hASC into insulin-producing cells.
Thu Dec 01 00:00:00 EST 2022
A Non-Nuclear NF-κB Modulates Alcohol Sensitivity But Not Immunity.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Wijesekera TP,Wu Z,Stephens NP,Godula R,Lew LK,Atkinson NS
A-11122 was used in Immunohistochemistry to demonstrate that, in Drosophila melanogaster, NF-κB action is important both inside and outside the nucleus and that the Dif gene has segregated nuclear and non-nuclear NF-κB action into different protein isoforms.
Wed Apr 20 00:00:00 EDT 2022
Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy.
Nature biomedical engineering
Sarkar D,Kang J,Wassie AT,Schroeder ME,Peng Z,Tarr TB,Tang AH,Niederst ED,Young JZ,Su H,Park D,Yin P,Tsai LH,Blanpied TA,Boyden ES
A-11122 was used in Immunocytochemistry-immunofluorescence to understand how proteins within these structures are arranged with nanoscale precision therefore requires that these structures be decrowded before labelling.
Thu Sep 01 00:00:00 EDT 2022
Dynamic Ins2 Gene Activity Defines β-Cell Maturity States.
Diabetes
Chu CMJ,Modi H,Ellis C,Krentz NAJ,Skovsø S,Zhao YB,Cen H,Noursadeghi N,Panzhinskiy E,Hu X,Dionne DA,Xia YH,Xuan S,Huising MO,Kieffer TJ,Lynn FC,Johnson JD
A-11122 was used in Immunocytochemistry-immunofluorescence to demonstrate that the heterogeneity of insulin production, observed in mouse and human β-cells, can be accounted for by dynamic states of insulin gene activity.
Thu Dec 01 00:00:00 EST 2022
Genetic analysis suggests a surface of PAT-4 (ILK) that interacts with UNC-112 (kindlin).
G3 (Bethesda, Md.)
Qadota H,McPherson A,Corbitt R,Dackowski EK,Matsunaga Y,Oberhauser AF,Benian GM
A-11122 was used in Immunohistochemistry-immunofluorescence to understand the molecular interaction between PAT-4 and UNC-112.
Wed Jul 06 00:00:00 EDT 2022
Microglial control of neuronal development via somatic purinergic junctions.
Cell reports
Cserép C,Schwarcz AD,Pósfai B,László ZI,Kellermayer A,Környei Z,Kisfali M,Nyerges M,Lele Z,Katona I
A-11122 was used in Immunohistochemistry-immunofluorescence to propose that early developmental formation of somatic purinergic junctions represents an important interface for microglia to monitor the status of immature neurons and control neurodevelopment.
Tue Sep 20 00:00:00 EDT 2022
The insect somatostatin pathway gates vitellogenesis progression during reproductive maturation and the post-mating response.
Nature communications
Zhang C,Kim AJ,Rivera-Perez C,Noriega FG,Kim YJ
A-11122 was used in Immunohistochemistry to identify the neuropeptide allatostatin-C (AstC)-producing neurons that gate both the initiation of vitellogenesis (yolk accumulation) that occurs post-eclosion and its re-initiation post-mating.
Fri Feb 18 00:00:00 EST 2022
A conserved sequence in the small intracellular loop of tetraspanins forms an M-shaped inter-helix turn.
Scientific reports
Reppert N,Lang T
A-11122 was used in Western Blot to explore the small intracellular loop between transmembrane segment 2 and 3 in tetraspanins.
Wed Mar 16 00:00:00 EDT 2022
Identification of Lsd1-interacting non-coding RNAs as regulators of fly oogenesis.
Cell reports
Shao TL,Ting RT,Lee MC
A-11122 was used in RNA Immunoprecipitation to present a physiological example of dynamic lncRNA-Lsd1 interplay that regulates stem cell/progenitor differentiation.
Tue Aug 30 00:00:00 EDT 2022
Deciphering the Molecular Interaction Between the Adhesion G Protein-Coupled Receptor ADGRV1 and its PDZ-Containing Regulator PDZD7.
Frontiers in molecular biosciences
Colcombet-Cazenave B,Cordier F,Zhu Y,Bouvier G,Litsardaki E,Laserre L,Prevost MS,Raynal B,Caillet-Saguy C,Wolff N
A-11122 was used in Pull-Down Assay to show that the stability and binding properties of the PDZ tandem are affected by two deafness-causing mutations located in the binding grooves of PDZD7 PDZ domains.
Sat Jul 16 00:00:00 EDT 2022
Proteomic and functional analyses of the periodic membrane skeleton in neurons.
Nature communications
Zhou R,Han B,Nowak R,Lu Y,Heller E,Xia C,Chishti AH,Fowler VM,Zhuang X
A-11122 was used in Immunohistochemistry to provide insights into the interactome of the MPS and suggest previously unknown functions of the MPS in neurons.
Thu Jun 09 00:00:00 EDT 2022
Genetic Inactivation of Notch1 Synergizes with Loss of Trp53 to Induce Tumor Formation in the Adult Mouse Forebrain.
Cancers
Parmigiani E,Giachino C
A-11122 was used in Immunohistochemistry-immunofluorescence to demonstrate that inactivation of Notch1 and Trp53 in cells with active Notch signaling is sufficient to induce brain tumor or hyperplasia formation.
Wed Nov 02 00:00:00 EDT 2022
Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging.
Nature methods
Chung KKH,Zhang Z,Kidd P,Zhang Y,Williams ND,Rollins B,Yang Y,Lin C,Baddeley D,Bewersdorf J
A-11122 was used in Immunocytochemistry-immunofluorescence to demonstrate a 26-fold increase in imaging speed over regular DNA-PAINT and show that our new implementation enables three-dimensional super-resolution DNA-PAINT imaging without optical sectioning.
Sun May 01 00:00:00 EDT 2022
Neuromuscular embodiment of feedback control elements in Drosophila flight.
Science advances
Whitehead SC,Leone S,Lindsay T,Meiselman MR,Cowan NJ,Dickinson MH,Yapici N,Stern DL,Shirangi T,Cohen I
A-11122 was used in Immunohistochemistry to provide evidence for an organizational principle in which each muscle contributes to a specific functional role in flight control, a finding that highlights the power of using top-down behavioral modeling to guide bottom-up cellular manipulation studies.
Wed Dec 14 00:00:00 EST 2022
Pathogenic mutations in the chromokinesin KIF22 disrupt anaphase chromosome segregation.
eLife
Thompson AF,Blackburn PR,Arons NS,Stevens SN,Babovic-Vuksanovic D,Lian JB,Klee EW,Stumpff J
A-11122 was used in Western Blotting to demonstrate the importance of both the head and tail of the motor in regulating the activity of KIF22 and offer insight into the cellular consequences of preventing KIF22 inactivation and disrupting force balance in anaphase.
Wed Jun 22 00:00:00 EDT 2022
Cholinergic interneuron inhibition potentiates corticostriatal transmission in direct medium spiny neurons and rescues motor learning in parkinsonism.
Cell reports
Laverne G,Pesce J,Reynders A,Combrisson E,Gascon E,Melon C,Kerkerian-Le Goff L,Maurice N,Beurrier C
A-11122 was used in Immunohistochemistry to demonstrate that the control exerted by CINs on corticostriatal transmission and striatal-dependent motor-skill learning depends on the integrity of dopaminergic inputs.
Tue Jul 05 00:00:00 EDT 2022
Combining mass spectrometry and genetic labeling in mice to report TRP channel expression.
MethodsX
Wartenberg P,Lux F,Busch K,Fecher-Trost C,Wyatt A,Flockerzi V,Krasteva-Christ G,Boehm U,Weissgerber P
A-11122 was used in Immunohistochemistry to present an optimized method to visualize primary TRP-expressing cells with single cell resolution throughout the entire organism.
Tue Dec 27 00:00:00 EST 2022
General and Specific Aversive Modulation of Active Avoidance Require Central Amygdala.
Frontiers in behavioral neuroscience
Kim IT,Farb C,Hou M,Prasad S,Talley E,Cook S,Campese VD
A-11122 was used in Immunocytochemistry-immunofluorescence to validate a role of central amygdala in producing aversive Pavlovian-instrumental transfer.
Sat Jul 16 00:00:00 EDT 2022
Microscopic and biochemical monitoring of endosomal trafficking and extracellular vesicle secretion in an endogenous in vivo model.
Journal of extracellular vesicles
Linnemannstöns K,Karuna M P,Witte L,Choezom D,Honemann-Capito M,Lagurin AS,Schmidt CV,Shrikhande S,Steinmetz LK,Wiebke M,Lenz C,Gross JC
A-11122 was used in Western Blotting to present an in vivo model to study EV secretion using the fat body and the haemolymph of the fruit fly, Drosophila melanogaster.
Thu Sep 01 00:00:00 EDT 2022
Structure and function differences in the prelimbic cortex to basolateral amygdala circuit mediate trait vulnerability in a novel model of acute social defeat stress in male mice.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
Grossman YS,Fillinger C,Manganaro A,Voren G,Waldman R,Zou T,Janssen WG,Kenny PJ,Dumitriu D
A-11122 was used in Immunohistochemistry-immunofluorescence to support PL?BLA circuit overactivity during stress as a biomarker of trait vulnerability and potential target for prevention of stress-induced psychopathology.
Tue Feb 01 00:00:00 EST 2022
Mutation in senataxin alters the mechanism of R-loop resolution in amyotrophic lateral sclerosis 4.
Brain : a journal of neurology
Kannan A,Cuartas J,Gangwani P,Branzei D,Gangwani L
A-11122 was used in Simple Western - Size to propose that ZPR1 collaborates with SETX and may function as a molecular brake to regulate SETX-dependent R-loop resolution activity critical for the normal functioning of motor neurons.
Wed Sep 14 00:00:00 EDT 2022
TRPC3 Antagonizes Pruritus in a Mouse Contact Dermatitis Model.
The Journal of investigative dermatology
Beattie K,Jiang H,Gautam M,MacVittie MK,Miller B,Ma M,Liu Q,Luo W
A-11122 was used in Immunohistochemistry-immunofluorescence to identify a role of TrpC3 and NP1 afferents in contact dermatitis pathology.
Fri Apr 01 00:00:00 EDT 2022
Neural network organization for courtship-song feature detection in Drosophila.
Current biology : CB
Baker CA,McKellar C,Pang R,Nern A,Dorkenwald S,Pacheco DA,Eckstein N,Funke J,Dickson BJ,Murthy M
A-11122 was used in Immunohistochemistry-immunofluorescence to study auditory activity in the Drosophila melanogaster brain and investigate feature selectivity for the two main modes of fly courtship song, sinusoids and pulse trains.
Mon Aug 08 00:00:00 EDT 2022
DMA-tudor interaction modules control the specificity of in vivo condensates.
Cell
Courchaine EM,Barentine AES,Straube K,Lee DR,Bewersdorf J,Neugebauer KM
A-11122 was used in Western Blotting to conclude that DMA-tudor interaction modules-combinations of tudor domains bound to their DMA ligand(s)-represent versatile yet specific regulators of MLO assembly, composition, and morphology.
Thu Jul 08 00:00:00 EDT 2021
A circuit logic for sexually shared and dimorphic aggressive behaviors in Drosophila.
Cell
Chiu H,Hoopfer ED,Coughlan ML,Pavlou HJ,Goodwin SF,Anderson DJ
A-11122 was used in Immunohistochemistry to reveal a circuit logic for the neural control of behaviors that include both sexually monomorphic and dimorphic actions, which may generalize to other organisms.
Thu Jan 21 00:00:00 EST 2021
Sneezing reflex is mediated by a peptidergic pathway from nose to brainstem.
Cell
Li F,Jiang H,Shen X,Yang W,Guo C,Wang Z,Xiao M,Cui L,Luo W,Kim BS,Chen Z,Huang AJW,Liu Q
A-11122 was used in Immunohistochemistry to hypothesize that a neuronal population postsynaptic to nasal sensory neurons mediates sneezing in this region.
Thu Jul 08 00:00:00 EDT 2021
Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.
Science (New York, N.Y.)
Gwon Y,Maxwell BA,Kolaitis RM,Zhang P,Kim HJ,Taylor JP
A-11122 was used in Western Blotting to investigate if targeting of G3BP1 weakened the stress granule-specific interaction network, resulting in granule disassembly.
Fri Jun 25 00:00:00 EDT 2021
Global mapping of Salmonella enterica-host protein-protein interactions during infection.
Cell host & microbe
Walch P,Selkrig J,Knodler LA,Rettel M,Stein F,Fernandez K,Viéitez C,Potel CM,Scholzen K,Geyer M,Rottner K,Steele-Mortimer O,Savitski MM,Holden DW,Typas A
A-11122 was used in Western Blotting to provide a method for probing host-pathogen PPIs during infection and a resource for interrogating STm effector mechanisms.
Wed Aug 11 00:00:00 EDT 2021
Sleep down state-active ID2/Nkx2.1 interneurons in the neocortex.
Nature neuroscience
Valero M,Viney TJ,Machold R,Mederos S,Zutshi I,Schuman B,Senzai Y,Rudy B,Buzsáki G
A-11122 was used in Immunohistochemistry to identify these down state-active (DSA) neurons as deep-layer neocortical neurogliaform cells that express ID2 and Nkx2.1 and are weakly immunoreactive to neuronal nitric oxide synthase. DSA neurons are weakly excited by deep-layer pyramidal cells and strongly inhibited by several other GABAergic cell types.
Mon Mar 01 00:00:00 EST 2021
Multi-omic analysis of selectively vulnerable motor neuron subtypes implicates altered lipid metabolism in ALS.
Nature neuroscience
Lee H,Lee JJ,Park NY,Dubey SK,Kim T,Ruan K,Lim SB,Park SH,Ha S,Kovlyagina I,Kim KT,Kim S,Oh Y,Kim H,Kang SU,Song MR,Lloyd TE,Maragakis NJ,Hong YB,Eoh H,Lee G
A-11122 was used in Immunohistochemistry to pinpoint a catalytic step of lipid metabolism as a potential therapeutic target for ALS.
Wed Dec 01 00:00:00 EST 2021
Transient suppression of transplanted spermatogonial stem cell differentiation restores fertility in mice.
Cell stem cell
Nakamura Y,Jörg DJ,Kon Y,Simons BD,Yoshida S
A-11122 was used in Immunocytochemistry to show that, after a large population of donor spermatogonia settle in host testes, through stochastic fate choice, only a small fraction persist and regenerate over the long term, and the rest are lost through differentiation and cell death.
Thu Aug 05 00:00:00 EDT 2021
A therapeutic cancer vaccine delivers antigens and adjuvants to lymphoid tissues using genetically modified T cells.
The Journal of clinical investigation
Veatch JR,Singhi N,Srivastava S,Szeto JL,Jesernig B,Stull SM,Fitzgibbon M,Sarvothama M,Yechan-Gunja S,James SE,Riddell SR
A-11122 was used in Immunohistochemistry to harness gene-modified T cells as a vaccine platform and developed cancer vaccines composed of autologous T cells modified with tumor antigens and additional adjuvant signals (Tvax).
Mon Aug 16 00:00:00 EDT 2021
A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization.
Molecular cell
Forey R,Barthe A,Tittel-Elmer M,Wery M,Barrault MB,Ducrot C,Seeber A,Krietenstein N,Szachnowski U,Skrzypczak M,Ginalski K,Rowicka M,Cobb JA,Rando OJ,Soutourina J,Werner M,Dubrana K,Gasser SM,Morillon A,Pasero P,Lengronne A,Poli J
A-11122 was used in ChIP assay to demonstrate a novel role for MRX in budding yeast transcription and chromosome organization.
Thu Jan 07 00:00:00 EST 2021
ESA1 regulates meiotic chromosome axis and crossover frequency via acetylating histone H4.
Nucleic acids research
Wang Y,Zhai B,Tan T,Yang X,Zhang J,Song M,Tan Y,Yang X,Chu T,Zhang S,Wang S,Zhang L
A-11122 was used in Immunocytochemistry to show Esa1, the NuA4 complex catalytic subunit, is constitutively expressed and localizes on chromatin loops during meiosis.
Mon Sep 20 00:00:00 EDT 2021
Proneural genes define ground-state rules to regulate neurogenic patterning and cortical folding.
Neuron
Han S,Okawa S,Wilkinson GA,Ghazale H,Adnani L,Dixit R,Tavares L,Faisal I,Brooks MJ,Cortay V,Zinyk D,Sivitilli A,Li S,Malik F,Ilnytskyy Y,Angarica VE,Gao J,Chinchalongporn V,Oproescu AM,Vasan L,Touahri Y,David LA,Raharjo E,Kim JW,Wu W,Rahmani W,Chan JA,Kovalchuk I,Attisano L,Kurrasch D,Dehay C,Swaroop A,Castro DS,Biernaskie J,Del Sol A,Schuurmans C
A-11122 was used in Immunohistochemistry to show that Neurog2 and Ascl1 proneural genes regulate cortical progenitor cell differentiation through crossrepressive interactions to sustain neurogenic continuity in a lissencephalic rodent brain.
Wed Sep 15 00:00:00 EDT 2021
Highly selective brain-to-gut communication via genetically defined vagus neurons.
Neuron
Tao J,Campbell JN,Tsai LT,Wu C,Liberles SD,Lowell BB
A-11122 was used in Immunohistochemistry to conclude that the vagus motor nerve communicates via genetically defined labeled lines to control functionally unique enteric neurons within discrete subregions of the gastrointestinal tract.
Wed Jul 07 00:00:00 EDT 2021
Adaptive cell invasion maintains lateral line organ homeostasis in response to environmental changes.
Developmental cell
Peloggia J,Münch D,Meneses-Giles P,Romero-Carvajal A,Lush ME,Lawson ND,McClain M,Pan YA,Piotrowski T
A-11122 was used in Immunohistochemistry to study cell invasion and migration, as well as the physiological adaptation of vertebrate organs to changing environmental conditions.
Mon May 03 00:00:00 EDT 2021
Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry.
Developmental cell
Lavalou J,Mao Q,Harmansa S,Kerridge S,Lellouch AC,Philippe JM,Audebert S,Camoin L,Lecuit T
A-11122 was used in Immunohistochemistry to reveal that Toll-8 and Cirl exhibit mutually dependent planar polarity in response to quantitative differences in Toll-8 expression between neighboring cells in Drosophila.
Mon Jun 07 00:00:00 EDT 2021
Metformin rescues muscle function in BAG3 myofibrillar myopathy models.
Autophagy
Ruparelia AA,McKaige EA,Williams C,Schulze KE,Fuchs M,Oorschot V,Lacene E,Meregalli M,Lee C,Serrano RJ,Baxter EC,Monro K,Torrente Y,Ramm G,Stojkovic T,Lavoie JN,Bryson-Richardson RJ
A-11122 was used in Immunocytochemistry to show BAG3P209L expression is sufficient to promote protein aggregation, it is the loss of BAG3 due to its sequestration within aggregates, which results in impaired autophagic activity, and subsequent muscle weakness.
Wed Sep 01 00:00:00 EDT 2021
Selective autophagy regulates heat stress memory in Arabidopsis by NBR1-mediated targeting of HSP90.1 and ROF1.
Autophagy
Thirumalaikumar VP,Gorka M,Schulz K,Masclaux-Daubresse C,Sampathkumar A,Skirycz A,Vierstra RD,Balazadeh S
A-11122 was used in Western Blotting to demonstrate that NBR1 (next to BRCA1 gene 1) plays a crucial role as a receptor for selective autophagy during recovery from HS.
Wed Sep 01 00:00:00 EDT 2021
Hugin+ neurons provide a link between sleep homeostat and circadian clock neurons.
Proceedings of the National Academy of Sciences of the United States of America
Schwarz JE,King AN,Hsu CT,Barber AF,Sehgal A
A-11122 was used in Immunohistochemistry to propose that hugin + neurons integrate circadian and sleep signals to modulate circadian circuitry and regulate the timing of sleep.
Tue Nov 23 00:00:00 EST 2021
PIEZO2 mediates ultrasonic hearing via cochlear outer hair cells in mice.
Proceedings of the National Academy of Sciences of the United States of America
Li J,Liu S,Song C,Hu Q,Zhao Z,Deng T,Wang Y,Zhu T,Zou L,Wang S,Chen J,Liu L,Hou H,Yuan K,Zheng H,Liu Z,Chen X,Sun W,Xiao B,Xiong W
A-11122 was used in Immunohistochemistry to demonstrate that OHCs serve as effector cells, combining with PIEZO2 as an essential molecule for ultrasonic hearing in mice.
Tue Jul 13 00:00:00 EDT 2021
A Fast and Accessible Method for the Isolation of RNA, DNA, and Protein To Facilitate the Detection of SARS-CoV-2.
Journal of clinical microbiology
Ponce-Rojas JC,Costello MS,Proctor DA,Kosik KS,Wilson MZ,Arias C,Acosta-Alvear D
A-11122 was used in Western Blotting to proposed Precipitation Enhanced Analyte RetrievaL as an alternative solution for SARS-CoV-2 testing that address RNA extraction kits bottlenecking.
Fri Mar 19 00:00:00 EDT 2021
Astroglial Calcium Signaling Encodes Sleep Need in Drosophila.
Current biology : CB
Blum ID,Keleş MF,Baz ES,Han E,Park K,Luu S,Issa H,Brown M,Ho MCW,Tabuchi M,Liu S,Wu MN
A-11122 was used in Immunocytochemistry to define astroglial Ca2+ signaling mechanisms encoding sleep need and reveal dynamic properties of the sleep homeostatic control system.
Mon Jan 11 00:00:00 EST 2021
Smoothelin-like 2 Inhibits Coronin-1B to Stabilize the Apical Actin Cortex during Epithelial Morphogenesis.
Current biology : CB
Hachimi M,Grabowski C,Campanario S,Herranz G,Baonza G,Serrador JM,Gomez-Lopez S,Barea MD,Bosch-Fortea M,Gilmour D,Bagnat M,Rodriguez-Fraticelli AE,Martin-Belmonte F
A-11122 was used in Immunocytochemistry to demonstrate the role of Smoothelin-like 2 in apical cortex maturation during epithelial morphogenesis.
Mon Feb 22 00:00:00 EST 2021
Smoothelin-like 2 Inhibits Coronin-1B to Stabilize the Apical Actin Cortex during Epithelial Morphogenesis.
Current biology : CB
Hachimi M,Grabowski C,Campanario S,Herranz G,Baonza G,Serrador JM,Gomez-Lopez S,Barea MD,Bosch-Fortea M,Gilmour D,Bagnat M,Rodriguez-Fraticelli AE,Martin-Belmonte F
A-11122 was used in Immunocytochemistry to demonstrate the role of Smoothelin-like 2 in apical cortex maturation during epithelial morphogenesis.
Mon Feb 22 00:00:00 EST 2021
Smoothelin-like 2 Inhibits Coronin-1B to Stabilize the Apical Actin Cortex during Epithelial Morphogenesis.
Current biology : CB
Hachimi M,Grabowski C,Campanario S,Herranz G,Baonza G,Serrador JM,Gomez-Lopez S,Barea MD,Bosch-Fortea M,Gilmour D,Bagnat M,Rodriguez-Fraticelli AE,Martin-Belmonte F
A-11122 was used in Immunocytochemistry to demonstrate the role of Smoothelin-like 2 in apical cortex maturation during epithelial morphogenesis.
Mon Feb 22 00:00:00 EST 2021
Smoothened transduces Hedgehog signals via activity-dependent sequestration of PKA catalytic subunits.
PLoS biology
Arveseth CD,Happ JT,Hedeen DS,Zhu JF,Capener JL,Klatt Shaw D,Deshpande I,Liang J,Xu J,Stubben SL,Nelson IB,Walker MF,Kawakami K,Inoue A,Krogan NJ,Grunwald DJ,Hüttenhain R,Manglik A,Myers BR
A-11122 was used in Western Blot to demonstrate a mechanism directly linking Hh signal transduction at the membrane to GLI transcription in the nucleus.
Thu Apr 01 00:00:00 EDT 2021
The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.
Cellular and molecular life sciences : CMLS
Castroflorio E,den Hoed J,Svistunova D,Finelli MJ,Cebrian-Serrano A,Corrochano S,Bassett AR,Davies B,Oliver PL
A-11122 was used in Immunocytochemistry to investigate the molecular function of nuclear receptor coactivator 7 in neurons and generated a novel mouse model to determine the consequences of deleting this locus in vivo.
Thu Apr 01 00:00:00 EDT 2021
Growth cone repulsion to Netrin-1 depends on lipid raft microdomains enriched in UNC5 receptors.
Cellular and molecular life sciences : CMLS
Hernaiz-Llorens M,Roselló-Busquets C,Durisic N,Filip A,Ulloa F,Martínez-Mármol R,Soriano E
A-11122 was used in Western Blot to indicate that the distribution of all UNC5 receptors into cholesterol-enriched raft microdomains is heterogeneous and that the specific localization has functional consequences for the axonal chemorepulsion against Netrin-1.
Mon Mar 01 00:00:00 EST 2021
A domesticated Harbinger transposase forms a complex with HDA6 and promotes histone H3 deacetylation at genes but not TEs in Arabidopsis.
Journal of integrative plant biology
Zhou X,He J,Velanis CN,Zhu Y,He Y,Tang K,Zhu M,Graser L,de Leau E,Wang X,Zhang L,Andy Tao W,Goodrich J,Zhu JK,Zhang CJ
A-11122 was used in Immunoprecipitation to suggest that Harbinger transposon-derived SANT domain-containing proteins are required for histone deacetylation and flowering time in plants.
Sun Aug 01 00:00:00 EDT 2021
Characterization of histone inheritance patterns in the Drosophila female germline.
EMBO reports
Kahney EW,Zion EH,Sohn L,Viets-Layng K,Johnston R,Chen X
A-11122 was used in Immunohistochemistry to combine histone labeling with DNA Oligopaints to distinguish old versus new histones and visualize their inheritance patterns at a single-gene resolution in asymmetrically dividing cells in vivo.
Mon Jul 05 00:00:00 EDT 2021
Poly(ADP-ribose) binding and macroH2A mediate recruitment and functions of KDM5A at DNA lesions.
The Journal of cell biology
Kumbhar R,Sanchez A,Perren J,Gong F,Corujo D,Medina F,Devanathan SK,Xhemalce B,Matouschek A,Buschbeck M,Buck-Koehntop BA,Miller KM
A-11122 was used in Immunoprecipitation to reveal the importance of PAR binding and histone variant macroH2A1.2 in KDM5A recognition of DNA lesion sites, driving transcriptional and repair activities at DNA breaks within chromatin that are essential for maintaining genome integrity.
Mon Jul 05 00:00:00 EDT 2021
Assembly of γ-secretase occurs through stable dimers after exit from the endoplasmic reticulum.
The Journal of cell biology
Wouters R,Michiels C,Sannerud R,Kleizen B,Dillen K,Vermeire W,Ayala AE,Demedts D,Schekman R,Annaert W
A-11122 was used in Immunoprecipitation to conclude that dimeric subcomplexes aided by Sec24 paralog selectivity support a stepwise assembly of γ-secretase, controlling final levels in post-Golgi compartments.
Mon Sep 06 00:00:00 EDT 2021
COPII collar defines the boundary between ER and ER exit site and does not coat cargo containers.
The Journal of cell biology
Shomron O,Nevo-Yassaf I,Aviad T,Yaffe Y,Zahavi EE,Dukhovny A,Perlson E,Brodsky I,Yeheskel A,Pasmanik-Chor M,Mironov A,Beznoussenko GV,Mironov AA,Sklan EH,Patterson GH,Yonemura Y,Sannai M,Kaether C,Hirschberg K
A-11122 was used in Western Blot to monitor cargo selection processes, accumulation, and fission of COPII-free ERES membranes.
Mon Jun 07 00:00:00 EDT 2021
GENOMES UNCOUPLED1-independent retrograde signaling targets the ethylene pathway to repress photomorphogenesis.
Plant physiology
Gommers CMM,Ruiz-Sola MÁ,Ayats A,Pereira L,Pujol M,Monte E
A-11122 was used in Western Blot to show how two apparently different stress responses converge to optimize photomorphogenesis.
Thu Feb 25 00:00:00 EST 2021
Delta opioid receptor regulation of calcitonin gene-related peptide dynamics in the trigeminal complex.
Pain
Moye LS,Siegersma K,Dripps I,Witkowski W,Mangutov E,Wang D,Scherrer G,Pradhan AA
A-11122 was used in Immunohistochemistry to suggest that DOP agonists inhibit migraine-associated pain by attenuating CGRP release and blocking pronociceptive signaling of the CGRPR.
Sun Aug 01 00:00:00 EDT 2021
The complete cell lineage and MAPK- and Otx-dependent specification of the dopaminergic cells in the Ciona brain.
Development (Cambridge, England)
Oonuma K,Kusakabe TG
A-11122 was used in Immunohistochemistry to establish the cellular and molecular framework for fully understanding the commitment to dopaminergic cells in the simple chordate brain.
Tue Jun 15 00:00:00 EDT 2021
A Sexually Dimorphic Olfactory Neuron Mediates Fixed Action Transition during Courtship Ritual in Drosophila melanogaster.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Tanaka NK,Hirao T,Chida H,Ejima A
A-11122 was used in Immunohistochemistry to show that lateral antennal lobe tract projection neuron 4 (lPN4) in D. melanogaster functions as an "AND-gate" for volatile and contact chemosensory inputs, mediating the expression of tapping behaviors before unilateral wing extension during male courtship rituals.
Wed Nov 24 00:00:00 EST 2021
A Basomedial Amygdala to Intercalated Cells Microcircuit Expressing PACAP and Its Receptor PAC1 Regulates Contextual Fear.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Rajbhandari AK,Octeau CJ,Gonzalez S,Pennington ZT,Mohamed F,Trott J,Chavez J,Ngyuen E,Keces N,Hong WZ,Neve RL,Waschek J,Khakh BS,Fanselow MS
A-11122 was used in Immunohistochemistry to show that a specific circuitry containing the neuropeptide PACAP and its receptor, PAC1, regulates various aspects of fear, including acquisition, generalization, recall, and extinction in a sexually dimorphic manner, characterizing a novel pathway that modulates traumatic fear.
Wed Apr 14 00:00:00 EDT 2021
Modulation of Both Intrinsic and Extrinsic Factors Additively Promotes Rewiring of Corticospinal Circuits after Spinal Cord Injury.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Nakamura Y,Ueno M,Niehaus JK,Lang RA,Zheng Y,Yoshida Y
A-11122 was used in Immunohistochemistry to demonstrate a potential molecular approach to reconstruct motor pathways after SCI.
Wed Dec 15 00:00:00 EST 2021
Developmental Regulation of Homeostatic Plasticity in Mouse Primary Visual Cortex.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Wen W,Turrigiano GG
A-11122 was used in Immunocytochemistry-immunoflourescence to suggest that homeostatic forms of plasticity can be recruited in a modular manner according to the evolving needs of a developing neural circuit.
Wed Dec 01 00:00:00 EST 2021
Structural and Functional Synaptic Plasticity Induced by Convergent Synapse Loss in the Drosophila Neuromuscular Circuit.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Wang Y,Lobb-Rabe M,Ashley J,Anand V,Carrillo RA
A-11122 was used in Immunocytochemistry to study Drosophila neuronal plasticity in response to synapse loss.
Wed Feb 17 00:00:00 EST 2021
MAGI-1 PDZ2 Domain Blockade Averts Adenovirus Infection via Enhanced Proteolysis of the Apical Coxsackievirus and Adenovirus Receptor.
Journal of virology
Alghamri MS,Sharma P,Williamson TL,Readler JM,Yan R,Rider SD,Hostetler HA,Cool DR,Kolawole AO,Excoffon KJDA
A-11122 was used in Western Blot to show that the interactions between the MAGI-1 PDZ2 domain and CAREx8 would decrease the apical CAREx8 expression level and prevent AdV infection.
Thu Jun 10 00:00:00 EDT 2021
Potent anti-tumor effects of receptor-retargeted syncytial oncolytic herpes simplex virus.
Molecular therapy oncolytics
Suzuki T,Uchida H,Shibata T,Sasaki Y,Ikeda H,Hamada-Uematsu M,Hamasaki R,Okuda K,Yanagi S,Tahara H
A-11122 was used in Immunohistochemistry to investigate the in vivo anti-tumor effects of a single-chain antibody for targeting tumor-associated antigens modified with additional mutations conferring syncytium formation - RRsyn-oHSV - using human cancer xenografts in immunodeficient mice.
Fri Sep 24 00:00:00 EDT 2021
Phosphorylation in two discrete tau domains regulates a stepwise process leading to postsynaptic dysfunction.
The Journal of physiology
Teravskis PJ,Oxnard BR,Miller EC,Kemper L,Ashe KH,Liao D
A-11122 was used in Immunocytochemistry-immunoflourescence to study how postsynaptic dysfunction results from a sequential process involving differential phosphorylation in the N-terminal and C-terminal domains in cultured rat hippocampal neurons.
Sat May 01 00:00:00 EDT 2021
Slit/Robo Signaling Regulates Multiple Stages of the Development of the Drosophila Motion Detection System.
Frontiers in cell and developmental biology
Guzmán-Palma P,Contreras EG,Mora N,Smith M,González-Ramírez MC,Campusano JM,Sierralta J,Hassan BA,Oliva C
A-11122 was used in Immunohistochemistry to reveal the co-option of the Slit-Robo signaling pathway in distinct developmental stages of a neural lineage.
Thu May 13 00:00:00 EDT 2021
The Wilms Tumor Gene wt1a Contributes to Blood-Cerebrospinal Fluid Barrier Function in Zebrafish.
Frontiers in cell and developmental biology
Hopfenmüller VL,Perner B,Reuter H,Bates TJD,Große A,Englert C
A-11122 was used in Immunohistochemistry to investigate expression domains for Wt1 in the doors hindbrain of zebrafish.
Sat Jan 29 00:00:00 EST 2022
Lyophilized yeast powder for adjuvant free thermostable vaccine delivery.
Applied microbiology and biotechnology
Kumar R,Kharbikar BN
A-11122 was used in a functional assay to show that E. coli curli-green fluorescent fusion protein remains stable in freeze-dried yeast powder for more than 18 and 12 months when stored at 30 °C and 37 °C respectively, to address the pressing need for the development of thermostable vaccines.
Thu Apr 01 00:00:00 EDT 2021
Endogenously expressed Ranbp2 is not at the axon initial segment.
Journal of cell science
Ogawa Y,Rasband MN
A-11122 was used in Immunocytochemistry-immunoflourescence to investigate if endogenously expressed Ranbp2 is not found at axon initial segments.
Thu Mar 11 00:00:00 EST 2021
Aberrant cytoplasmic localization of ARID1B activates ERK signaling and promotes oncogenesis.
Journal of cell science
Animireddy S,Kavadipula P,Kotapalli V,Gowrishankar S,Rao S,Bashyam MD
A-11122 was used in Immunocytochemistry to characterize the ARID1B nuclear localization signal.
Fri Feb 26 00:00:00 EST 2021
Rostro-Caudal Specificity of Corticospinal Tract Projections in Mice.
Cerebral cortex (New York, N.Y. : 1991)
Steward O,Yee KM,Metcalfe M,Willenberg R,Luo J,Azevedo R,Martin-Thompson JH,Gandhi SP
A-11122 was used in Immunocytochemistry-immunoflourescence to assess rostro-caudal specificity of corticospinal tract projections from different areas of the cortex by retrograde labeling with fluorogold and retrograde transfection following retro-AAV/Cre injection into the spinal cord of tdT reporter mice.
Wed Mar 31 00:00:00 EDT 2021
Maturation of Complex Synaptic Connections of Layer 5 Cortical Axons in the Posterior Thalamic Nucleus Requires SNAP25.
Cerebral cortex (New York, N.Y. : 1991)
Hayashi S,Hoerder-Suabedissen A,Kiyokage E,Maclachlan C,Toida K,Knott G,Molnár Z
A-11122 was used in Immunomicroscopy to study synapse maturation in the absence of regulated vesicular release.
Wed Mar 31 00:00:00 EDT 2021
Gene Transfer in Adeno-Associated Virus Seropositive Rhesus Macaques Following Rapamycin Treatment and Subcutaneous Delivery of AAV6, but Not Retargeted AAV6 Vectors.
Human gene therapy
Stone D,Kenkel EJ,Loprieno MA,Tanaka M,De Silva Feelixge HS,Kumar AJ,Stensland L,Obenza WM,Wangari S,Ahrens CY,Murnane RD,Peterson CW,Kiem HP,Huang ML,Aubert M,Hu SL,Jerome KR
A-11122 was used in Immunohistochemistry to investigate whether immunosuppression would allow gene delivery by AAV6 or targeted AAV6 derivatives in seropositive rhesus macaques.
Fri Jan 01 00:00:00 EST 2021
Intracerebroventricular Administration of AAV9-PHP.B SYN1-EmGFP Induces Widespread Transgene Expression in the Mouse and Monkey Central Nervous System.
Human gene therapy
Galvan A,Petkau TL,Hill AM,Korecki AJ,Lu G,Choi D,Rahman K,Simpson EM,Leavitt BR,Smith Y
A-11122 was used in Immunohistochemistry to demonstrate that a single injection in the lateral ventricle is an efficient method to obtain transgene expression in many cortical and subcortical regions, obviating the need of multiple intraparenchymal injections to cover large brain areas.
Tue Jun 01 00:00:00 EDT 2021
A biallelic pathogenic variant in the OGDH gene results in a neurological disorder with features of a mitochondrial disease.
Journal of inherited metabolic disease
Yap ZY,Strucinska K,Matsuzaki S,Lee S,Si Y,Humphries K,Tarnopolsky MA,Yoon WH
A-11122 was used in Western Blotting to identify two individuals carrying a homozygous missense variant c.959A>G (p.N320S) in the 2-Oxoglutarate dehydrogenase gene.
Mon Mar 01 00:00:00 EST 2021
Variants of human CLDN9 cause mild to profound hearing loss.
Human mutation
Ramzan M,Philippe C,Belyantseva IA,Nakano Y,Fenollar-Ferrer C,Tona R,Yousaf R,Basheer R,Imtiaz A,Faridi R,Munir Z,Idrees H,Salman M,Nambot S,Vitobello A,Kartti S,Zarrik O,Witmer PD,Sobreria N,Ibrahimi A,Banfi B,Moutton S,Friedman TB,Naz S
A-11122 was used in Western Blot to expand the genotype-phenotype spectrum of CLDN9-associated deafness and describe two different homozygous variants in individuals from two unrelated families affected with prelingual or adolescent-onset hearing loss.
Fri Oct 01 00:00:00 EDT 2021
Conserved and divergent aspects of Robo receptor signaling and regulation between Drosophila Robo1 and C. elegans SAX-3.
Genetics
Daiber T,VanderZwan-Butler CJ,Bashaw GJ,Evans TA
A-11122 was used in Immunohistochemistry to show that SAX-3 is not subject to endosomal sorting by the negative regulator Commissureless (Comm) in Drosophila neurons in vivo, and that peri-membrane and ectodomain sequences are both required for Comm sorting of Drosophila Robo1.
Wed Mar 31 00:00:00 EDT 2021
Dynein regulates Kv7.4 channel trafficking from the cell membrane.
The Journal of general physiology
van der Horst J,Rognant S,Abbott GW,Ozhathil LC,Hägglund P,Barrese V,Chuang CY,Jespersen T,Davies MJ,Greenwood IA,Gourdon P,Aalkjær C,Jepps TA
A-11122 was used in Western Blot to study the influence of dynein on the microtubule-dependent internalization of the voltage-gated Kv7.4 potassium channel.
Mon Mar 01 00:00:00 EST 2021
Contribution of Serotonin 3A Receptor to Motor Function and Its Expression in the Gastrointestinal Tract.
Digestion
Aikiyo S,Kishi K,Kaji N,Mikawa S,Kondo M,Shimada S,Hori M
A-11122 was used in Immunocytochemistry to show that 5-HT3AR is highly expressed in the stomach and large intestine and that the activation of 5-HT3AR accelerates gastric emptying and large intestine transit.
Wed Aug 18 00:00:00 EDT 2021
Aversive teaching signals from individual dopamine neurons in larval Drosophila show qualitative differences in their temporal "fingerprint".
The Journal of comparative neurology
Weiglein A,Thoener J,Feldbruegge I,Warzog L,Mancini N,Schleyer M,Gerber B
A-11122 was used in Immunohistochemistry to suggest that heterogeneity in the temporal fingerprint of teaching signals might be a more general principle of reinforcement processing through dopamine neurons.
Sat May 01 00:00:00 EDT 2021
CRISPR-Cas9 gene editing of hepatitis B virus in chronically infected humanized mice.
Molecular therapy. Methods & clinical development
Stone D,Long KR,Loprieno MA,De Silva Feelixge HS,Kenkel EJ,Liley RM,Rapp S,Roychoudhury P,Nguyen T,Stensland L,Colón-Thillet R,Klouser LM,Weber ND,Le C,Wagoner J,Goecker EA,Li AZ,Eichholz K,Corey L,Tyrrell DL,Greninger AL,Huang ML,Polyak SJ,Aubert M,Sagartz JE,Jerome KR
A-11122 was used in Immunohistochemistry to provide evidence for the feasibility and safety of in vivo gene editing for chronic HBV infections.
Fri Mar 12 00:00:00 EST 2021
Competitive coordination of the dual roles of the Hedgehog co-receptor in homophilic adhesion and signal reception.
eLife
Yang S,Zhang Y,Yang C,Wu X,El Oud SM,Chen R,Cai X,Wu XS,Lan G,Zheng X
A-11122 was used in Immunocytochemistry-immunoflourescence to report that Interference hedgehog (Ihog) not only forms a Hh receptor complex with Patched to mediate intracellular signaling, but Ihog also engages in trans-homophilic binding leading to cytoneme stabilization in a manner independent of its role as the Hh receptor.
Tue May 18 00:00:00 EDT 2021
Generation of a Novel Nkx6-1 Venus Fusion Reporter Mouse Line.
International journal of molecular sciences
Burtscher I,Tarquis-Medina M,Salinno C,Schirge S,Beckenbauer J,Bakhti M,Lickert H
A-11122 was used in Western Blot to summarize that the generation of the Nkx6-1-VF reporter line reflects the expression pattern and dynamics of the endogenous protein and thus provides a unique tool to study the spatio-temporal expression pattern of this TF during organ development and enables isolation and tracking of Nkx6-1-expressing cells such as pancreatic β-cells, but also neurons and motor neurons in health and disease.
Fri Mar 26 00:00:00 EDT 2021
Neuropathy-causing TRPV4 mutations disrupt TRPV4-RhoA interactions and impair neurite extension.
Nature communications
McCray BA,Diehl E,Sullivan JM,Aisenberg WH,Zaccor NW,Lau AR,Rich DJ,Goretzki B,Hellmich UA,Lloyd TE,Sumner CJ
A-11122 was used in Western Blot to identify RhoA as a critical mediator of TRPV4-induced cell structure changes and suggest that disruption of TRPV4-RhoA binding may contribute to tissue-specific toxicity of TRPV4 neuropathy mutations.
Thu Mar 04 00:00:00 EST 2021
Combinatorial deployment of F-actin regulators to build complex 3D actin structures in vivo.
eLife
Xie Y,Budhathoki R,Blankenship JT
A-11122 was used in Immunohistochemistry to present an in-depth characterization of cortical actin cap dynamics and their regulation in vivo.
Wed May 05 00:00:00 EDT 2021
The endocycle restores tissue tension in the Drosophila abdomen post wound repair.
Cell reports
Losick VP,Duhaime LG
A-11122 was used in Immunohistochemistry to find that the wound-induced polyploid cells remodel the epithelium to maintain fly abdominal movements, which may help compensate for lost tissue tension.
Tue Oct 12 00:00:00 EDT 2021
insomniac links the development and function of a sleep-regulatory circuit.
eLife
Li Q,Jang H,Lim KY,Lessing A,Stavropoulos N
A-11122 was used in Immunohistochemistry to link neurogenesis and postmitotic development of sleep-regulatory neurons to their adult function and suggest that developmental perturbations of circuits that couple sensory inputs and sleep may underlie sleep dysfunction in neurodevelopmental disorders.
Wed Dec 15 00:00:00 EST 2021
Light-triggered and phosphorylation-dependent 14-3-3 association with NON-PHOTOTROPIC HYPOCOTYL 3 is required for hypocotyl phototropism.
Nature communications
Reuter L,Schmidt T,Manishankar P,Throm C,Keicher J,Bock A,Droste-Borel I,Oecking C
A-11122 was used in Western Blot to demonstrate that blue light induces an immediate phosphorylation of a C-terminal 14-3-3 binding motif in NPH3.
Thu Oct 21 00:00:00 EDT 2021
The histone acetyltransferase HBO1 promotes efficient tip cell sprouting during angiogenesis.
Development (Cambridge, England)
Grant ZL,Hickey PF,Abeysekera W,Whitehead L,Lewis SM,Symons RCA,Baldwin TM,Amann-Zalcenstein D,Garnham AL,Naik SH,Smyth GK,Thomas T,Voss AK,Coultas L
A-11122 was used in Immunohistochemistry to investigate the role of the histone acetyltransferase HBO1 (KAT7), which is important for activating genes during development and for histone H3 lysine 14 acetylation (H3K14ac).
Fri Oct 15 00:00:00 EDT 2021
ALS2 regulates endosomal trafficking, postsynaptic development, and neuronal survival.
The Journal of cell biology
Kim J,Kim S,Nahm M,Li TN,Lin HC,Kim YD,Lee J,Yao CK,Lee S
A-11122 was used in Western Blotting to demonstrate that dALS2 directs early to late endosome trafficking and that the dFz2 C terminus is cleaved in late endosomes.
Mon May 03 00:00:00 EDT 2021
Identification of Two Dysfunctional Variants in the ABCG2 Urate Transporter Associated with Pediatric-Onset of Familial Hyperuricemia and Early-Onset Gout.
International journal of molecular sciences
Toyoda Y,Pavelcová K,Bohatá J,Ješina P,Kubota Y,Suzuki H,Takada T,Stiburkova B
A-11122 was used in Western Blotting to describe a representative case of familial pediatric-onset hyperuricemia and early-onset gout associated with a dysfunctional ABCG2, i.e., a clinical history of three generations of one Czech family with biochemical and molecular genetic findings.
Tue Feb 16 00:00:00 EST 2021
Human Respiratory Syncytial Virus Subgroup A and B Infections in Nasal, Bronchial, Small-Airway, and Organoid-Derived Respiratory Cultures.
mSphere
Rijsbergen LC,Lamers MM,Comvalius AD,Koutstaal RW,Schipper D,Duprex WP,Haagmans BL,de Vries RD,de Swart RL
A-11122 was used in Immunohistochemistry (Paraffin) to compare the replicative fitness and HRSV-induced innate cytokine responses of HRSV-A and HRSV-B strains in disease-relevant cell culture models.
Wed May 12 00:00:00 EDT 2021
Slowpoke functions in circadian output cells to regulate rest:activity rhythms.
PloS one
Ruiz D,Bajwa ST,Vanani N,Bajwa TA,Cavanaugh DJ
A-11122 was used in Immunohistochemistry to demonstrate that slo exerts its effects in multiple components of the circadian circuit, including PI output cells in addition to clock neurons, and we hypothesize that it does so by contributing to the generation of daily neuronal activity rhythms that allow for the propagation of circadian information throughout output circuits.
Tue Oct 19 00:00:00 EDT 2021
Expression of FoxP2 in the basal ganglia regulates vocal motor sequences in the adult songbird.
Nature communications
Xiao L,Merullo DP,Koch TMI,Cao M,Co M,Kulkarni A,Konopka G,Roberts TF
A-11122 was used in Western Blot to demonstrate discrete circuit origins for the dysfluent repetition of vocal elements in songbirds, with implications for speech disorders.
Tue May 11 00:00:00 EDT 2021
Expression of FoxP2 in the basal ganglia regulates vocal motor sequences in the adult songbird.
Nature communications
Xiao L,Merullo DP,Koch TMI,Cao M,Co M,Kulkarni A,Konopka G,Roberts TF
A-11122 was used in Western Blot to demonstrate discrete circuit origins for the dysfluent repetition of vocal elements in songbirds, with implications for speech disorders.
Tue May 11 00:00:00 EDT 2021
Pancreatic Ppy-expressing γ-cells display mixed phenotypic traits and the adaptive plasticity to engage insulin production.
Nature communications
Perez-Frances M,van Gurp L,Abate MV,Cigliola V,Furuyama K,Bru-Tari E,Oropeza D,Carreaux T,Fujitani Y,Thorel F,Herrera PL
A-11122 was used in Immunohistochemistry to present the genetic signature of human and mouse γ-cells.
Thu Jul 22 00:00:00 EDT 2021
A locomotor neural circuit persists and functions similarly in larvae and adult Drosophila.
eLife
Lee K,Doe CQ
A-11122 was used in Immunocytochemistry to utilize a well-characterized neural circuit in the Drosophila larva: the moonwalker descending neuron (MDN) circuit.
Wed Jul 14 00:00:00 EDT 2021
Plexin-B2 controls the timing of differentiation and the motility of cerebellar granule neurons.
eLife
Van Battum E,Heitz-Marchaland C,Zagar Y,Fouquet S,Kuner R,Chédotal A
A-11122 was used in Immunocytochemistry to suggest that Plexin-B2 restricts CGN motility and might have a function in cytokinesis.
Tue Jun 08 00:00:00 EDT 2021
The steroid-hormone ecdysone coordinates parallel pupariation neuromotor and morphogenetic subprograms via epidermis-to-neuron Dilp8-Lgr3 signal induction.
Nature communications
Heredia F,Volonté Y,Pereirinha J,Fernandez-Acosta M,Casimiro AP,Belém CG,Viegas F,Tanaka K,Menezes J,Arana M,Cardoso GA,Macedo A,Kotowicz M,Prado Spalm FH,Dibo MJ,Monfardini RD,Torres TT,Mendes CS,Garelli A,Gontijo AM
A-11122 was used in Immunohistochemistry to reveal that interorgan feedback gates progression between subunits of an innate behavior and points to an ancestral neuromodulatory function of relaxin signaling.
Mon Jun 07 00:00:00 EDT 2021
Replication dynamics of recombination-dependent replication forks.
Nature communications
Naiman K,Campillo-Funollet E,Watson AT,Budden A,Miyabe I,Carr AM
A-11122 was used in ChIP assay to show that the leading strand initiates replication at the same position, signifying the stability of the 3' single strand in the context of increased resection.
Wed Feb 10 00:00:00 EST 2021
Cell proliferation fate mapping reveals regional cardiomyocyte cell-cycle activity in subendocardial muscle of left ventricle.
Nature communications
Liu X,Pu W,He L,Li Y,Zhao H,Li Y,Liu K,Huang X,Weng W,Wang QD,Shen L,Zhong T,Sun K,Ardehali R,He B,Zhou B
A-11122 was used in Immunocytochemistry to develop a genetic approach for a temporally seamless recording of cardiomyocyte-specific cell-cycle activity in vivo.
Fri Oct 01 00:00:00 EDT 2021
A Heart Failure-Associated SCN5A Splice Variant Leads to a Reduction in Sodium Current Through Coupled-Gating With the Wild-Type Channel.
Frontiers in physiology
Zheng Y,Wan X,Yang D,Ramirez-Navarro A,Liu H,Fu JD,Deschênes I
A-11122 was used in Immunoprecipitation to summarize that the HF-associated splice variant Nav1.5-G1642X suppresses sodium currents in heart failure patients through a mechanism involving coupled-gating with the wildtype sodium channel.
Sat Apr 10 00:00:00 EDT 2021
A neural circuit integrates pharyngeal sensation to control feeding.
Cell reports
Yang T,Yuan Z,Liu C,Liu T,Zhang W
A-11122 was used in Immunohistochemistry to reveal the function of pharyngeal mechanoreceptors and their downstream neural circuits in the control of food ingestion.
Tue Nov 09 00:00:00 EST 2021
Profilin and Mical combine to impair F-actin assembly and promote disassembly and remodeling.
Nature communications
Grintsevich EE,Ahmed G,Ginosyan AA,Wu H,Rich SK,Reisler E,Terman JR
A-11122 was used in In vivo experiments to conclude that profilin and Mical combine to impair F-actin assembly and promote F-actin disassembly, while concomitantly facilitating cellular remodeling and plasticity.
Mon Sep 20 00:00:00 EDT 2021
The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition.
Cell reports
Azzoni E,Frontera V,Anselmi G,Rode C,James C,Deltcheva EM,Demian AS,Brown J,Barone C,Patelli A,Harman JR,Nicholls M,Conway SJ,Morrissey E,Jacobsen SEW,Sparrow DB,Harris AL,Enver T,de Bruijn MFTR
A-11122 was used in Immunocytochemistry-immunoflourescence to investigate how and when the onset of circulation affects Hematopoietic stem cells emergence.
Tue Dec 14 00:00:00 EST 2021
Persistent DNA damage signaling and DNA polymerase theta promote broken chromosome segregation.
The Journal of cell biology
Clay DE,Bretscher HS,Jezuit EA,Bush KB,Fox DT
A-11122 was used in Immunohistochemistry-immunofluorescence to reveal a mechanism for how cells with inactive DSB checkpoints can respond to DNA damage that persists into mitosis.
Mon Dec 06 00:00:00 EST 2021
Cryptosporidium rhoptry effector protein ROP1 injected during invasion targets the host cytoskeletal modulator LMO7.
Cell host & microbe
Guérin A,Roy NH,Kugler EM,Berry L,Burkhardt JK,Shin JB,Striepen B
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, In Vitro Assay to delineate the events of host cell invasion and uncover a mechanism unique to Cryptosporidium.
Wed Sep 08 00:00:00 EDT 2021
Cryptosporidium rhoptry effector protein ROP1 injected during invasion targets the host cytoskeletal modulator LMO7.
Cell host & microbe
Guérin A,Roy NH,Kugler EM,Berry L,Burkhardt JK,Shin JB,Striepen B
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, In Vitro Assay to delineate the events of host cell invasion and uncover a mechanism unique to Cryptosporidium.
Wed Sep 08 00:00:00 EDT 2021
A midbrain dynorphin circuit promotes threat generalization.
Current biology : CB
Fellinger L,Jo YS,Hunker AC,Soden ME,Elum J,Juarez B,Zweifel LS
A-11122 was used in Immunohistochemistry to demonstrate that conditioned threat discrimination has an inverted "U" relationship with threat intensity and establish a role for KOR/Dyn signaling in the midbrain for promoting threat generalization.
Mon Oct 11 00:00:00 EDT 2021
piRNA-independent transposon silencing by the Drosophila THO complex.
Developmental cell
Zhang G,Yu T,Parhad SS,Ho S,Weng Z,Theurkauf WE
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot to show that the thoc7-null mutant combination increases transposon transcription but does not reduce anti-sense piRNAs targeting half of the transcriptionally activated transposon families.
Mon Sep 27 00:00:00 EDT 2021
A multi-gene knockdown approach reveals a new role for Pax6 in controlling organ number in Drosophila.
Development (Cambridge, England)
Ordway AJ,Teeters GM,Weasner BM,Weasner BP,Policastro R,Kumar JP
A-11122 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry, Western Blot to investigate Pax6 regulatory roles in drosophila, in organ number regulation.
Sat May 01 00:00:00 EDT 2021
Neonicotinoids disrupt memory, circadian behaviour and sleep.
Scientific reports
Tasman K,Hidalgo S,Zhu B,Rands SA,Hodge JJL
A-11122 was used in Immunohistochemistry to show that disruption of learning, circadian rhythmicity and sleep are likely to have far-reaching detrimental effects on beneficial insects in the field.
Thu Jan 21 00:00:00 EST 2021
Antagonistic roles for Ataxin-2 structured and disordered domains in RNP condensation.
eLife
Singh A,Hulsmeier J,Kandi AR,Pothapragada SS,Hillebrand J,Petrauskas A,Agrawal K,Rt K,Thiagarajan D,Jayaprakashappa D,VijayRaghavan K,Ramaswami M,Bakthavachalu B
A-11122 was used in Immunohistochemistry to increase the understanding of neuronal translational control mechanisms and inform strategies for Atx2-based interventions under development for neurodegenerative disease.
Wed Mar 10 00:00:00 EST 2021
Functional interpretation of ATAD3A variants in neuro-mitochondrial phenotypes.
Genome medicine
Yap ZY,Park YH,Wortmann SB,Gunning AC,Ezer S,Lee S,Duraine L,Wilichowski E,Wilson K,Mayr JA,Wagner M,Li H,Kini U,Black ED,Monaghan KG,Lupski JR,Ellard S,Westphal DS,Harel T,Yoon WH
A-11122 was used in Immunohistochemistry to expand the allelic spectrum of ATAD3A variants and exemplify the use of a functional assay in Drosophila to aid variant interpretation.
Mon Apr 12 00:00:00 EDT 2021
fruitless tunes functional flexibility of courtship circuitry during development.
eLife
Chen J,Jin S,Chen D,Cao J,Ji X,Peng Q,Pan Y
A-11122 was used in Immunohistochemistry to show that fruM is not absolutely necessary for courtship but is critical during development to build a sex circuitry with reduced flexibility and enhanced efficiency, and provide a new view about how fruM tunes functional flexibility of a sex circuitry instead of switching on its function as conventionally viewed.
Tue Jan 19 00:00:00 EST 2021
Gene-environment interactions mediate stress susceptibility and resilience through the CaMKIIβ/TARPγ-8/AMPAR pathway.
iScience
Sakai Y,Li H,Inaba H,Funayama Y,Ishimori E,Kawatake-Kuno A,Yamagata H,Seki T,Hobara T,Nakagawa S,Watanabe Y,Tomita S,Murai T,Uchida S
A-11122 was used in Immunohistochemistry to elucidate the mechanisms of ventral hippocampus and gene-environment cross-talk implicated in the pathophysiology of depression.
Fri May 21 00:00:00 EDT 2021
Aortic disease in Marfan syndrome is caused by overactivation of sGC-PRKG signaling by NO.
Nature communications
de la Fuente-Alonso A,Toral M,Alfayate A,Ruiz-Rodríguez MJ,Bonzón-Kulichenko E,Teixido-Tura G,Martínez-Martínez S,Méndez-Olivares MJ,López-Maderuelo D,González-Valdés I,Garcia-Izquierdo E,Mingo S,Martín CE,Muiño-Mosquera L,De Backer J,Nistal JF,Forteza A,Evangelista A,Vázquez J,Campanero MR,Redondo JM
A-11122 was used in Immunohistochemistry to identify potential biomarkers for monitoring Marfan Syndrome in patients and urge evaluation of cGMP-dependent protein kinase and soluble guanylate cyclase as therapeutic targets.
Tue May 11 00:00:00 EDT 2021
BMPR-2 gates activity-dependent stabilization of primary dendrites during mitral cell remodeling.
Cell reports
Aihara S,Fujimoto S,Sakaguchi R,Imai T
A-11122 was used in Immunohistochemistry to screen for cell surface receptors that control the dendrite remodeling process in mitral cells.
Tue Jun 22 00:00:00 EDT 2021
Screening of a Focused Ubiquitin-Proteasome Pathway Inhibitor Library Identifies Small Molecules as Novel Modulators of Botulinum Neurotoxin Type A Toxicity.
Frontiers in pharmacology
Sen E,Kota KP,Panchal RG,Bavari S,Kiris E
A-11122 was used in Western Blotting to study a focused ubiquitin-proteasome pathway inhibitor library and identified potential lead compounds affecting BoNT/A mediated SNAP-25 cleavage in neurons in pre-intoxication conditions.
Fri Oct 15 00:00:00 EDT 2021
Mitochondrial Defects in Fibroblasts of Pathogenic MAPT Patients.
Frontiers in cell and developmental biology
Bharat V,Hsieh CH,Wang X
A-11122 was used in Western Blotting to report that, in MAPT patients' fibroblasts, disease-causing MAPT mutations compromise early events of mitophagy.
Fri Feb 24 00:00:00 EST 2023
Transmembrane topology and oligomeric nature of an astrocytic membrane protein, MLC1.
Open biology
Hwang J,Park K,Lee GY,Yoon BY,Kim H,Roh SH,Lee BC,Kim K,Lim HH
A-11122 was used in Immunocytochemistry-immunofluorescence to provide a basis for both the high-resolution structural determination and functional characterization of the MLC1 protein.
Wed Dec 01 00:00:00 EST 2021
The CBP/p300 histone acetyltransferases function as plant-specific MEDIATOR subunits in Arabidopsis.
Journal of integrative plant biology
Guo J,Wei L,Chen SS,Cai XW,Su YN,Li L,Chen S,He XJ
A-11122 was used in Immunohistochemistry-immunofluorescence to identify all of the components of Arabidopsis MEDIATOR and reveal the mechanism underlying the link between histone acetylation and transcriptional regulation.
Thu Apr 01 00:00:00 EDT 2021
Chromosome oscillation promotes Aurora A-dependent Hec1 phosphorylation and mitotic fidelity.
The Journal of cell biology
Iemura K,Natsume T,Maehara K,Kanemaki MT,Tanaka K
A-11122 was used in Western Blotting to propose that Aurora A-mediated metaphase Hec1-S55 phosphorylation through chromosome oscillation, together with Hec1-S69 phosphorylation, ensures mitotic fidelity by eliminating erroneous kinetochore-microtubule attachments.
Mon Jul 05 00:00:00 EDT 2021
Metallo-β-lactamase domain-containing protein 2 is S-palmitoylated and exhibits acyl-CoA hydrolase activity.
The Journal of biological chemistry
Malgapo MIP,Safadi JM,Linder ME
A-11122 was used in Western Blotting to confirm that MBLAC2 is palmitoylated and identify the likely S-palmitoylation site as Cys254.
Tue Aug 24 00:00:00 EDT 2021
Self-organized patterning of cell morphology via mechanosensitive feedback.
eLife
Dye NA,Popović M,Iyer KV,Fuhrmann JF,Piscitello-Gómez R,Eaton S,Jülicher F
A-11122 was used in Immunocytochemistry to present a continuum theory showing that this pattern of cell morphology and tissue stress can arise via self-organization of a mechanical feedback that couples cell polarity to active cell rearrangements.
Fri Mar 26 00:00:00 EDT 2021
Neurofibromin regulates metabolic rate via neuronal mechanisms in Drosophila.
Nature communications
Botero V,Stanhope BA,Brown EB,Grenci EC,Boto T,Park SJ,King LB,Murphy KR,Colodner KJ,Walker JA,Keene AC,Ja WW,Tomchik SM
A-11122 was used in Western Blot to indicate that neurofibromin regulates metabolic rate via neuronal mechanisms, suggest that cellular and systemic metabolic alterations may represent a pathophysiological mechanism in neurofibromatosis type 1, and provide a platform for investigating the cellular role of neurofibromin in metabolic homeostasis.
Tue Jul 13 00:00:00 EDT 2021
Midbrain dopaminergic inputs gate amygdala intercalated cell clusters by distinct and cooperative mechanisms in male mice.
eLife
Aksoy-Aksel A,Gall A,Seewald A,Ferraguti F,Ehrlich I
A-11122 was used in Immunocytochemistry to provide novel insights into dopaminergic mechanisms shaping the activity balance between distinct ITC clusters that could support their opposing roles in fear behavior.
Mon May 24 00:00:00 EDT 2021
α-Synuclein-induced dysregulation of neuronal activity contributes to murine dopamine neuron vulnerability.
NPJ Parkinson's disease
Dagra A,Miller DR,Lin M,Gopinath A,Shaerzadeh F,Harris S,Sorrentino ZA,Støier JF,Velasco S,Azar J,Alonge AR,Lebowitz JJ,Ulm B,Bu M,Hansen CA,Urs N,Giasson BI,Khoshbouei H
A-11122 was used in Immunocytochemistry to provide mechanistic insights into the insidious changes in dopaminergic neuronal activity and neuronal loss that characterize Parkinson''s disease progression with significant therapeutic implications.
Wed Aug 18 00:00:00 EDT 2021
Rapid evolution of blood-brain-barrier-penetrating AAV capsids by RNA-driven biopanning.
Molecular therapy. Methods & clinical development
Nonnenmacher M,Wang W,Child MA,Ren XQ,Huang C,Ren AZ,Tocci J,Chen Q,Bittner K,Tyson K,Pande N,Chung CH,Paul SM,Hou J
A-11122 was used in Immunohistochemistry to developed a directed evolution approach based on recovery of capsid library RNA transcribed from CNS-restricted promoters to identified engineered capsids with improved blood-brain barrier (BBB) penetration and CNS tropism.
Fri Mar 12 00:00:00 EST 2021
Male-biased protein expression in primordial germ cells, identified through a comparative study of UAS vectors in Drosophila.
Scientific reports
Masukawa M,Ishizaki Y,Miura H,Hayashi M,Ota R,Kobayashi S
A-11122 was used in Immunohistochemistry to show that all three types of UAS vectors were functional in the germline cells of Drosophila embryos and larvae and that the UASt and UASz vectors were active in the germline of the distal tip region in adult testes.
Tue Nov 02 00:00:00 EDT 2021
Disrupted-in-schizophrenia 1 enhances the quality of circadian rhythm by stabilizing BMAL1.
Translational psychiatry
Lee SB,Park J,Kwak Y,Park YU,Nhung TTM,Suh BK,Woo Y,Suh Y,Cho E,Cho S,Park SK
A-11122 was used in Immunoprecipitation to demonstrate that the oscillation of DISC1 expression is under the control of CLOCK and BMAL1, and that DISC1 contributes to the core circadian system by regulating BMAL1 stability.
Thu Feb 04 00:00:00 EST 2021
A novel nitroalkene vitamin E analogue inhibits the NLRP3 inflammasome and protects against inflammation and glucose intolerance triggered by obesity.
Redox biology
Dapueto R,Rodriguez-Duarte J,Galliussi G,Kamaid A,Bresque M,Batthyány C,López GV,Escande C
A-11122 was used in Immunohistochemistry-wholemount to propose that this novel nitroalkene-Trolox derivative is a suitable tool to tackle acute and chronic inflammation in vitro and in vivo mainly due to inhibition of NF-kB/NLRP3 activation.
Mon Feb 01 00:00:00 EST 2021
Opposing functions of the plant TOPLESS gene family during SNC1-mediated autoimmunity.
PLoS genetics
Garner CM,Spears BJ,Su J,Cseke LJ,Smith SN,Rogan CJ,Gassmann W
A-11122 was used in Immunoprecipitation to show the diverse functions of the transcriptional co-repressor family TOPLESS in Arabidopsis.
Mon Feb 01 00:00:00 EST 2021
Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion.
eLife
Chaudhari K,Gorla M,Chang C,Kania A,Bashaw GJ
A-11122 was used in Western Blot to investigate the heteropentameric Scar/Wave Regulatory Complex as an effector of Robo signalling.
Mon Apr 12 00:00:00 EDT 2021
Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion.
eLife
Chaudhari K,Gorla M,Chang C,Kania A,Bashaw GJ
A-11122 was used in Western Blot to investigate the heteropentameric Scar/Wave Regulatory Complex as an effector of Robo signalling.
Mon Apr 12 00:00:00 EDT 2021
Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion.
eLife
Chaudhari K,Gorla M,Chang C,Kania A,Bashaw GJ
A-11122 was used in Western Blot to investigate the heteropentameric Scar/Wave Regulatory Complex as an effector of Robo signalling.
Mon Apr 12 00:00:00 EDT 2021
Novelty-like activation of locus coeruleus protects against deleterious human pretangle tau effects while stress-inducing activation worsens its effects.
Alzheimer's & dementia (New York, N. Y.)
Omoluabi T,Torraville SE,Maziar A,Ghosh A,Power KD,Reinhardt C,Harley CW,Yuan Q
A-11122 was used in Immunohistochemistry to argue that variations in environmental experiences associated with differing LC activity patterns may account for individual susceptibility to development of AD in humans.
Tue Jan 11 00:00:00 EST 2022
Amygdala Underlies the Environment Dependency of Defense Responses Induced via Superior Colliculus.
Frontiers in neural circuits
Isa K,Tokuoka K,Ikeda S,Karimi S,Kobayashi K,Sooksawate T,Isa T
A-11122 was used in Immunohistochemistry to test a hypothesis that the amygdala is involved in such environment dependency of the innate defense responses.
Thu Jan 27 00:00:00 EST 2022
Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish.
Biology open
Lepanto P,Levin-Ferreyra F,Koziol U,Malacrida L,Badano JL
A-11122 was used in Immunohistochemistry to present the characterization of a novel adipocyte-specific label in zebrafish and uncovers previously unknown aspects of in vivo adipogenesis.
Wed Sep 15 00:00:00 EDT 2021
A Brainstem reticulotegmental neural ensemble drives acoustic startle reflexes.
Nature communications
Guo W,Fan S,Xiao D,Dong H,Xu G,Wan Z,Ma Y,Wang Z,Xue T,Zhou Y,Li Y,Xiong W
A-11122 was used in Immunohistochemistry to show a functional role of the reticulotegmental nucleus in startle reflex.
Thu Nov 04 00:00:00 EDT 2021
Distinct Opsin 3 (Opn3) Expression in the Developing Nervous System during Mammalian Embryogenesis.
eNeuro
Davies WIL,Sghari S,Upton BA,Nord C,Hahn M,Ahlgren U,Lang RA,Gunhaga L
A-11122 was used in Immunohistochemistry to monitor cell body expression and axonal projections during embryonic and early postnatal to adult stages.
Wed Oct 20 00:00:00 EDT 2021
Distinct Expression Patterns of Cxcl12 in Mesenchymal Stem Cell Niches of Intact and Injured Rodent Teeth.
International journal of molecular sciences
Pagella P,Nombela-Arrieta C,Mitsiadis TA
A-11122 was used in Immunohistochemistry to show that Cxcl12 is widely expressed in intact and injured teeth and highlight its importance as a key component of the various dental mesenchymal stem cell niches.
Tue Mar 16 00:00:00 EDT 2021
Astrocytic ApoE underlies maturation of hippocampal neurons and cognitive recovery after traumatic brain injury in mice.
Communications biology
Yu TS,Tensaouti Y,Stephanz EP,Chintamen S,Rafikian EE,Yang M,Kernie SG
A-11122 was used in Immunohistochemistry to demonstrate that astrocytic ApoE is required for functional injury-induced neurogenesis following traumatic brain injury.
Thu Nov 18 00:00:00 EST 2021
SdhA blocks disruption of the Legionella-containing vacuole by hijacking the OCRL phosphatase.
Cell reports
Choi WY,Kim S,Aurass P,Huo W,Creasey EA,Edwards M,Lowe M,Isberg RR
A-11122 was used in Pull-Down Assay to show that SdhA hijacking of OCRL is associated with blocking trafficking events that disrupt the pathogen vacuole.
Tue Nov 02 00:00:00 EDT 2021
Bimolecular Fluorescence Complementation (BiFC) and Multiplexed Imaging of Protein-Protein Interactions in Human Living Cells.
Methods in molecular biology (Clifton, N.J.)
Jia Y,Bleicher F,Reboulet J,Merabet S
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, Inhibition Assay to analyze protein-protein interactions in live human cultured cells via bimolecular fluorescence complementation (BiFC).
Wed Sep 01 00:00:00 EDT 2021
Dorsal clock neurons in Drosophila sculpt locomotor outputs but are dispensable for circadian activity rhythms.
iScience
Nettnin EA,Sallese TR,Nasseri A,Saurabh S,Cavanaugh DJ
A-11122 was used in Immunohistochemistry-immunofluorescence to clarify the role of the dorsal neuron 1 (DN1) population of clock neurons in the transmission of circadian information by the Drosophila core clock network.
Fri Sep 24 00:00:00 EDT 2021
Differential requirement of MED14/17 recruitment for activation of heat inducible genes.
The New phytologist
Ohama N,Moo TL,Chua NH
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation to highlight the importance of differential recruitment of Mediator for the precise control of HS responses in plants.
Mon Mar 01 00:00:00 EST 2021
Arabidopsis seedling establishment under waterlogging requires ABCG5-mediated formation of a dense cuticle layer.
The New phytologist
Lee EJ,Kim KY,Zhang J,Yamaoka Y,Gao P,Kim H,Hwang JU,Suh MC,Kang B,Lee Y
A-11122 was used in Western Blotting to indicate that proper development of plants under waterlogged conditions requires the dense cuticle layer formed by ABCG5 activity.
Fri Jan 01 00:00:00 EST 2021
Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila.
Cell reports
Matinyan N,Karkhanis MS,Gonzalez Y,Jain A,Saltzman A,Malovannaya A,Sarrion-Perdigones A,Dierick HA,Venken KJT
A-11122 was used in Immunohistochemistry-immunofluorescence to describe a drug-based genetic platform consisting of four selection and two counterselection markers, eliminating the need to screen for modified progeny.
Tue Sep 14 00:00:00 EDT 2021
Chronic exercise training activates histone turnover in mouse skeletal muscle fibers.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Ohsawa I,Kawano F
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, ChIP Assay to reveal that 4 weeks of running training activates histone turnover in skeletal muscle fibers, loosening the nucleosomes and enhancing gene responses to exercise.
Thu Apr 01 00:00:00 EDT 2021
Chromatin architecture at susceptible gene loci in cerebellar Purkinje cells characterizes DNA damage-induced neurodegeneration.
Science advances
Kwak YD,Shaw TI,Downing SM,Tewari A,Jin H,Li Y,Dumitrache LC,Katyal S,Khodakhah K,Russell HR,McKinnon PJ
A-11122 was used in Western Blotting to reveal the pathogenic basis of DNA damage in the nervous system and suggest chromatin conformation as a feature in directing genome instability-associated neuropathology.
Fri Dec 17 00:00:00 EST 2021
Cannabinoid and planar cell polarity signaling converges to direct placentation.
Proceedings of the National Academy of Sciences of the United States of America
Kim YS,Li Y,Yuan J,Borg JP,Sun X,Dey SK
A-11122 was used in Western Blotting to show that organized PCP in trophoblasts is indispensable for their directed movement and that CB1 exerts its function by direct interaction with membrane proteins other than its canonical G protein-coupled receptor role.
Tue Sep 21 00:00:00 EDT 2021
Mutations in Drosophila crinkled/Myosin VIIA disrupt denticle morphogenesis.
Developmental biology
Sallee JL,Crawford JM,Singh V,Kiehart DP
A-11122 was used in Immunocytochemistry-immunofluorescence to indicate that ck/MyoVIIA plays a key role in the formation and/or organization of actin filament bundles, which drive proper shape of cellular projections.
Mon Feb 01 00:00:00 EST 2021
The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region.
Scientific reports
Kozono T,Sato H,Okumura W,Jogano C,Tamura-Nakano M,Kawamura YI,Rohrer J,Tonozuka T,Nishikawa A
A-11122 was used in Western Blotting to show that the N-terminal region of Jaw1 inhibits the formation of OSER as an IDR to maintain the homeostatic localization of interactors on the ER membrane.
Tue Jan 12 00:00:00 EST 2021
Telomeric double-strand DNA-binding proteins DTN-1 and DTN-2 ensure germline immortality in Caenorhabditis elegans.
eLife
Yamamoto I,Zhang K,Zhang J,Vorontsov E,Shibuya H
A-11122 was used in Western Blot, Immunocytochemistry to suggest that DTN-1 and DTN-2 are core shelterin components in C. elegans telomeres that act as negative regulators of telomere length and are essential for germline immortality.
Thu Jan 21 00:00:00 EST 2021
Telomeric double-strand DNA-binding proteins DTN-1 and DTN-2 ensure germline immortality in Caenorhabditis elegans.
eLife
Yamamoto I,Zhang K,Zhang J,Vorontsov E,Shibuya H
A-11122 was used in Western Blot, Immunocytochemistry to suggest that DTN-1 and DTN-2 are core shelterin components in C. elegans telomeres that act as negative regulators of telomere length and are essential for germline immortality.
Thu Jan 21 00:00:00 EST 2021
RTP801/REDD1 contributes to neuroinflammation severity and memory impairments in Alzheimer's disease.
Cell death & disease
Pérez-Sisqués L,Sancho-Balsells A,Solana-Balaguer J,Campoy-Campos G,Vives-Isern M,Soler-Palazón F,Anglada-Huguet M,López-Toledano MÁ,Mandelkow EM,Alberch J,Giralt A,Malagelada C
A-11122 was used in Western Blot to investigate the involvement of RTP801 in the pathophysiology of Alzheimer's disease.
Tue Jun 15 00:00:00 EDT 2021
Male courtship song drives escape responses that are suppressed for successful mating.
Scientific reports
Arez E,Mezzera C,Neto-Silva RM,Aranha MM,Dias S,Moita MA,Vasconcelos ML
A-11122 was used in Western Blot to suggest that courtship song has a dual role, promoting both escape and pause in females and that escape is suppressed by the activity of 70A09 neurons, allowing mating to occur.
Thu Apr 29 00:00:00 EDT 2021
TDP-43 proteinopathy alters the ribosome association of multiple mRNAs including the glypican Dally-like protein (Dlp)/GPC6.
Acta neuropathologica communications
Lehmkuhl EM,Loganathan S,Alsop E,Blythe AD,Kovalik T,Mortimore NP,Barrameda D,Kueth C,Eck RJ,Siddegowda BB,Joardar A,Ball H,Macias ME,Bowser R,Van Keuren-Jensen K,Zarnescu DC
A-11122 was used in RNA Immunoprecipitation to investigate if Dlp/GPC6 is a novel, physiologically relevant target of TDP-43 proteinopathy.
Wed Mar 24 00:00:00 EDT 2021
Perturbation of amygdala-cortical projections reduces ensemble coherence of palatability coding in gustatory cortex.
eLife
Lin JY,Mukherjee N,Bernstein MJ,Katz DB
A-11122 was used in Immunohistochemistry to suggest that BLA 'organizes' behavior-related GC taste dynamics.
Fri May 21 00:00:00 EDT 2021
BRCA2 binding through a cryptic repeated motif to HSF2BP oligomers does not impact meiotic recombination.
Nature communications
Ghouil R,Miron S,Koornneef L,Veerman J,Paul MW,Le Du MH,Sleddens-Linkels E,van Rossum-Fikkert SE,van Loon Y,Felipe-Medina N,Pendas AM,Maas A,Essers J,Legrand P,Baarends WM,Kanaar R,Zinn-Justin S,Zelensky AN
A-11122 was used in Western Blot to investigate if the high-affinity oligomerisation-inducing BRCA2-HSF2BP interaction is required for RAD51 and DMC1 recombinase localization in meiotic homologous recombination.
Thu Jul 29 00:00:00 EDT 2021
RNA polymerase backtracking results in the accumulation of fission yeast condensin at active genes.
Life science alliance
Rivosecchi J,Jost D,Vachez L,Gautier FD,Bernard P,Vanoosthuyse V
A-11122 was used in ChIP assay to strengthen the idea that dense arrays of proteins tightly bound to DNA alter the distribution of condensin on chromosomes.
Tue Jun 01 00:00:00 EDT 2021
Neuroglian regulates Drosophila intestinal stem cell proliferation through enhanced signaling via the epidermal growth factor receptor.
Stem cell reports
Resnik-Docampo M,Cunningham KM,Ruvalcaba SM,Choi C,Sauer V,Jones DL
A-11122 was used in Immunohistochemistry to show that the septate junction (SJ) protein Neuroglian (Nrg) is expressed in intestinal stem cells (ISCs) and enteroblasts (EBs) within the fly intestine.
Tue Jun 08 00:00:00 EDT 2021
RDC complex executes a dynamic piRNA program during Drosophila spermatogenesis to safeguard male fertility.
PLoS genetics
Chen P,Luo Y,Aravin AA
A-11122 was used in Chromatin immunoprecipitation to investigate how piRNA cluster transcription is regulated in the male germline.
Wed Sep 01 00:00:00 EDT 2021
Sorting Nexin 27 Enables MTOC and Secretory Machinery Translocation to the Immune Synapse.
Frontiers in immunology
González-Mancha N,Rodríguez-Rodríguez C,Alcover A,Merida I
A-11122 was used in Western Blot to show that sorting nexin 27 modulates immune synapse organisation through regulated trafficking of cargoes in T lymphocytes.
Mon Mar 07 00:00:00 EST 2022
Using the Drosophila Transcriptional Reporter of Intracellular Calcium (TRIC) to examine lasting ethanol-induced changes in neuroexcitability.
microPublication biology
Merriman K,Petruccelli E
A-11122 was used in Immunohistochemistry to examine pan-neuronal and dopaminergic cells with transcriptional reporter of intracellular calcium to test the hypothesis that ethanol exposure causes lasting changes in adult drosophila brain neuroexcitability.
Thu Sep 30 00:00:00 EDT 2021
Missense mutation of a conserved residue in UNC-112 (kindlin) eliminates binding to PAT-4 (ILK).
microPublication biology
Qadota H,Oberhauser AF,Benian GM
A-11122 was used in Immunohistochemistry to report identification of a novel UNC-112 mutation of a conserved residue that cannot bind to PAT-4.
Thu Sep 23 00:00:00 EDT 2021
Cyclical phosphorylation of LRAP35a and CLASP2 by GSK3β and CK1δ regulates EB1-dependent MT dynamics in cell migration.
Cell reports
Chia S,Leung T,Tan I
A-11122 was used in Immunocytochemistry-immunofluorescence to reveal an essential role for LRAP35a in coordinating lamellar contractility and microtubule polarization in cell migration.
Tue Sep 14 00:00:00 EDT 2021
Gene therapy for tuberous sclerosis complex type 2 in a mouse model by delivery of AAV9 encoding a condensed form of tuberin.
Science advances
Cheah PS,Prabhakar S,Yellen D,Beauchamp RL,Zhang X,Kasamatsu S,Bronson RT,Thiele EA,Kwiatkowski DJ,Stemmer-Rachamimov A,György B,Ling KH,Kaneki M,Tannous BA,Ramesh V,Maguire CA,Breakefield XO
A-11122 was used in Immunocytochemistry-immunofluorescence to demonstrate the potential of treating life-threatening TSC2 lesions with a single intravenous injection of AAV9-cTuberin.
Fri Jan 01 00:00:00 EST 2021
Downregulation of glial genes involved in synaptic function mitigates Huntington's disease pathogenesis.
eLife
Onur TS,Laitman A,Zhao H,Keyho R,Kim H,Wang J,Mair M,Wang H,Li L,Perez A,de Haro M,Wan YW,Allen G,Lu B,Al-Ramahi I,Liu Z,Botas J
A-11122 was used in Western Blotting to investigate the contributions of glia to Huntington's disease.
Mon Apr 19 00:00:00 EDT 2021
Metastasis suppressor 1 interacts with α-actinin 4 to affect its localization and regulate formation of membrane ruffling.
Cytoskeleton (Hoboken, N.J.)
Liang L,Liang X,Jiang P,Zhou L,Zhong L,Wang M,Lin S,Guo Z,Yu J,Yang C,Chen Y,Zhuo C,Chen P,Wang Y
A-11122 was used in Western Blotting to indicate that MTSS1 might recruit cytoplasmic ACTN4 to the cell periphery and regulate cytoskeleton dynamics to restrict its performance in membrane ruffling.
Thu Jul 01 00:00:00 EDT 2021
Metastasis suppressor 1 interacts with α-actinin 4 to affect its localization and regulate formation of membrane ruffling.
Cytoskeleton (Hoboken, N.J.)
Liang L,Liang X,Jiang P,Zhou L,Zhong L,Wang M,Lin S,Guo Z,Yu J,Yang C,Chen Y,Zhuo C,Chen P,Wang Y
A-11122 was used in Western Blotting to indicate that MTSS1 might recruit cytoplasmic ACTN4 to the cell periphery and regulate cytoskeleton dynamics to restrict its performance in membrane ruffling.
Thu Jul 01 00:00:00 EDT 2021
δ-Protocadherins regulate neural progenitor cell division by antagonizing Ryk and Wnt/β-catenin signaling.
iScience
Biswas S,Emond MR,Chenoweth KP,Jontes JD
A-11122 was used in Western Blotting to find that δ-protocadherins are novel regulators of Wnt/β-catenin signaling that may control the development of neural circuits by defining a molecular code for the identity of neural progenitor cells and differentially regulating their proliferation.
Fri Aug 20 00:00:00 EDT 2021
Capsid-specific nanobody effects on HIV-1 assembly and infectivity.
Virology
Alfadhli A,Romanaggi C,Barklis RL,Merutka I,Bates TA,Tafesse FG,Barklis E
A-11122 was used in Western Blotting to demonstrate the feasibility of Gag-targeted nanobody inhibition of HIV-1.
Fri Oct 01 00:00:00 EDT 2021
RAPGEF2 mediates oligomeric Aβ-induced synaptic loss and cognitive dysfunction in the 3xTg-AD mouse model of Alzheimer's disease.
Neuropathology and applied neurobiology
Jang YN,Jang H,Kim GH,Noh JE,Chang KA,Lee KJ
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot to demonstrate that the upregulation of RAPGEF2 levels mediates Aβ oligomer-induced synaptic and cognitive disturbances in the AD hippocampus.
Sun Aug 01 00:00:00 EDT 2021
Hedgehog signaling and Tre1 regulate actin dynamics through PI(4,5)P2 to direct migration of Drosophila embryonic germ cells.
Cell reports
Kim JH,Hanlon CD,Vohra S,Devreotes PN,Andrew DJ
A-11122 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry, Western Blot to propose that Tre1 functions as a master helmsman in germ cell (GC) navigation by integrating extracellular signals to induce intracellular changes.
Tue Mar 02 00:00:00 EST 2021
Loss of Jag1 cooperates with oncogenic Kras to induce pancreatic cystic neoplasms.
Life science alliance
Chung WC,Challagundla L,Zhou Y,Li M,Atfi A,Xu K
A-11122 was used in Immunohistochemistry to conclude that Jag1 can act as a tumor suppressor in the pancreas by delaying precursor lesions, whereas loss of Jag1 promoted a phenotypic switch from malignant carcinoma to benign cystic lesions.
Mon Feb 01 00:00:00 EST 2021
Interplay between Pds5 and Rec8 in regulating chromosome axis length and crossover frequency.
Science advances
Song M,Zhai B,Yang X,Tan T,Wang Y,Yang X,Tan Y,Chu T,Cao Y,Song Y,Wang S,Zhang L
A-11122 was used in Immunocytochemistry-immunoflourescence to show that only ~20% of wild-type level of Pds5 is required for homolog pairing and that higher levels of Pds5 dosage-dependently regulate axis length and crossover frequency.
Mon Mar 01 00:00:00 EST 2021
Importance of the renal ion channel TRPM6 in the circadian secretion of renin to raise blood pressure.
Nature communications
Funato Y,Yamazaki D,Okuzaki D,Yamamoto N,Miki H
A-11122 was used in Western Blot to indicate crucial roles of TRPM6 in the circadian regulation of blood pressure.
Thu Jun 17 00:00:00 EDT 2021
DEAD-box helicases modulate dicing body formation in Arabidopsis.
Science advances
Li Q,Liu N,Liu Q,Zheng X,Lu L,Gao W,Liu Y,Liu Y,Zhang S,Wang Q,Pan J,Chen C,Mi Y,Yang M,Cheng X,Ren G,Yuan YW,Zhang X
A-11122 was used in Immunohistochemistry to reveal the key roles of RH6, RH8, and RH12 in modulating D-body formation via LLPSs.
Thu Apr 01 00:00:00 EDT 2021
Molecular and cellular basis of acid taste sensation in Drosophila.
Nature communications
Mi T,Mack JO,Lee CM,Zhang YV
A-11122 was used in Immunocytochemistry to investigate how animals discriminate low from high acidity foods.
Thu Jun 17 00:00:00 EDT 2021
Optimized Immunostaining of Embryonic and Early Postnatal Mouse Brain Sections.
Bio-protocol
Harb K,Bertacchi M,Studer M
A-11122 was used in Immunohistochemistry to present a detailed protocol, suitable for both thin cryostat sections and thick vibratome sections, which has successfully worked for a wide range of antibodies directed against key molecular players of neocortical development.
Tue Jan 05 00:00:00 EST 2021
A neurogenic microenvironment defined by excitatory-inhibitory neuronal circuits in adult dentate gyrus.
Cell reports
Uemura M,Blankvoort S,Tok SSL,Yuan L,Cobar LF,Lit KK,Tashiro A
A-11122 was used in Immunohistochemistry to suggest that the behaviorally relevant activity of excitatory-inhibitory neuronal circuits can define a microenvironment stimulating/inhibiting neurogenesis.
Tue Jul 06 00:00:00 EDT 2021
Fibroblast-derived IL-33 is dispensable for lymph node homeostasis but critical for CD8 T-cell responses to acute and chronic viral infection.
European journal of immunology
Aparicio-Domingo P,Cannelle H,Buechler MB,Nguyen S,Kallert SM,Favre S,Alouche N,Papazian N,Ludewig B,Cupedo T,Pinschewer DD,Turley SJ,Luther SA
A-11122 was used in Immunohistochemistry-immunofluorescence to show that LN T-zone fibroblastic reticular cells (FRC) not only regulate the homeostasis of naive T cells but also their expansion and differentiation several days into an antiviral response.
Fri Jan 01 00:00:00 EST 2021
Magnesium and Calcium Homeostasis Depend on KCTD1 Function in the Distal Nephron.
Cell reports
Marneros AG
A-11122 was used in Immunohistochemistry to conclude that KCTD1 regulates urinary reabsorption of Mg2+ and Ca2+ by inducing expression of NCC in DCTs and NKCC2/claudin-16/-19 in TALs.
Tue Jan 12 00:00:00 EST 2021
A novel strategy of selective gene delivery by using a uniform magnetic field.
Biotechnology journal
Dateki M,Imamura O,Arai M,Shimizu H,Takishima K
A-11122 was used in Immunocytochemistry-immunofluorescence to establish a novel procedure for uniform magnetic field-dependent enhancement of transfection efficiency. We examined the effect of a 1.5 mT uniform magnetic field on cellular reactive oxygen species (ROS) level and transfection efficiency mediated by a ROS-sensitive transfection carrier.
Thu Apr 01 00:00:00 EDT 2021
Comprehensive topographical map of the serotonergic fibers in the male mouse brain.
The Journal of comparative neurology
Awasthi JR,Tamada K,Overton ETN,Takumi T
A-11122 was used in Immunohistochemistry-immunofluorescence to provide novel, regionally specific insights on the distribution map of serotonergic fibers using transgenic mouse.
Sat May 01 00:00:00 EDT 2021
Schizophrenia-associated dysbindin modulates axonal mitochondrial movement in cooperation with p150glued.
Molecular brain
Suh BK,Lee SA,Park C,Suh Y,Kim SJ,Woo Y,Nhung TTM,Lee SB,Mun DJ,Goo BS,Choi HS,Kim SJ,Park SK
A-11122 was used in Western Blotting to suggest that dysbindin forms a functional complex with p150glued that regulates axonal mitochondrial transport, thereby affecting presynaptic calcium homeostasis.
Mon Jan 18 00:00:00 EST 2021
A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS.
eLife
Mark B,Lai SL,Zarin AA,Manning L,Pollington HQ,Litwin-Kumar A,Cardona A,Truman JW,Doe CQ
A-11122 was used in Immunohistochemistry to demonstrate that the mechanisms specifying neuronal diversity also govern circuit formation and function, and that these principles are broadly applicable throughout the nervous system.
Tue May 11 00:00:00 EDT 2021
Use of high-content imaging to quantify transduction of AAV-PHP viruses in the brain following systemic delivery.
Brain communications
Smith EJ,Farshim PP,Flomen R,Jones ST,McAteer SJ,Deverman BE,Gradinaru V,Bates GP
A-11122 was used in Immunohistochemistry to allow the rapid comparison of transduction rates between brain regions producing comparable data to more time-consuming approaches.
Thu Mar 10 00:00:00 EST 2022
Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K+ channels.
eLife
Stevens SR,Longley CM,Ogawa Y,Teliska LH,Arumanayagam AS,Nair S,Oses-Prieto JA,Burlingame AL,Cykowski MD,Xue M,Rasband MN
A-11122 was used in Western Blot to investigate if Ankyrin-R regulates Pv+ fast-spiking interneuron function by organizing ion channels, cell adhesion molecules, and perineuronal nets, and linking these to the underlying β1 spectrin-based cytoskeleton.
Mon Jun 28 00:00:00 EDT 2021
Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle.
Science advances
Yoshioka K,Nagahisa H,Miura F,Araki H,Kamei Y,Kitajima Y,Seko D,Nogami J,Tsuchiya Y,Okazaki N,Yonekura A,Ohba S,Sumita Y,Chiba K,Ito K,Asahina I,Ogawa Y,Ito T,Ohkawa Y,Ono Y
A-11122 was used in Immunocytochemistry to investigate if Hox gene expression profiles instill the embryonic history in satellite cells as positional memory, potentially modulating region-specific pathophysiology in adult muscles.
Tue Jun 01 00:00:00 EDT 2021
The bZIP Transcription Factor HapX Is Post-Translationally Regulated to Control Iron Homeostasis in Aspergillus fumigatus.
International journal of molecular sciences
López-Berges MS,Scheven MT,Hortschansky P,Misslinger M,Baldin C,Gsaller F,Werner ER,Krüger T,Kniemeyer O,Weber J,Brakhage AA,Haas H
A-11122 was used in Western Blot to elucidate the role of the bZIP transcription factor, HapX, in the regulation of iron homeostasis in Aspergillus fumigatus.
Tue Jul 20 00:00:00 EDT 2021
FoxG1 regulates the formation of cortical GABAergic circuit during an early postnatal critical period resulting in autism spectrum disorder-like phenotypes.
Nature communications
Miyoshi G,Ueta Y,Natsubori A,Hiraga K,Osaki H,Yagasaki Y,Kishi Y,Yanagawa Y,Fishell G,Machold RP,Miyata M
A-11122 was used in Immunohistochemistry to study dysregulation of FOXG1 expression and how this contributes to autism spectrum disorder-like phenotypes.
Fri Jun 18 00:00:00 EDT 2021
Lymphocyte access to lymphoma is impaired by high endothelial venule regression.
Cell reports
Menzel L,Zschummel M,Crowley T,Franke V,Grau M,Ulbricht C,Hauser A,Siffrin V,Bajénoff M,Acton SE,Akalin A,Lenz G,Willimsky G,Höpken UE,Rehm A
A-11122 was used in Immunohistochemistry to show that lymphoma causes severe high endothelial venule regression in mouse models that phenocopies aggressive human B cell lymphomas.
Tue Oct 26 00:00:00 EDT 2021
Anti-Aggregative Effect of the Antioxidant DJ-1 on the TPPP/p25-Derived Pathological Associations of Alpha-Synuclein.
Cells
Oláh J,Lehotzky A,Szénási T,Ovádi J
A-11122 was used in Western Blot to reveal the protective effect of DJ-1 against toxic SYN assemblies, which assigns a new function to the antioxidant sensor DJ-1.
Wed Oct 27 00:00:00 EDT 2021
Surface Modification of E. coli Outer Membrane Vesicles with Glycosylphosphatidylinositol-Anchored Proteins: Generating Pro/Eukaryote Chimera Constructs.
Membranes
Zaruba M,Roschitz L,Sami H,Ogris M,Gerner W,Metzner C
A-11122 was used in Western Blot to show the functional surface modification of E. coli outer membrane vesicles with glycosylphosphatidylinositol (GPI)-anchored protein, exploiting a process described as molecular painting or protein engineering in eukaryotic membranes, whereby the lipid part of the GPI anchor inserts in cell membranes.
Fri Jun 04 00:00:00 EDT 2021
Pupal behavior emerges from unstructured muscle activity in response to neuromodulation in Drosophila.
eLife
Elliott AD,Berndt A,Houpert M,Roy S,Scott RL,Chow CC,Shroff H,White BH
A-11122 was used in Immunohistochemistry to provide a platform for studying whole-animal behavior, quantifying its variability across multiple spatiotemporal scales, and analyzing its neuromodulatory regulation at cellular resolution.
Thu Jul 08 00:00:00 EDT 2021
Parahippocampal latrophilin-2 (ADGRL2) expression controls topographical presubiculum to entorhinal cortex circuit connectivity.
Cell reports
Donohue JD,Amidon RF,Murphy TR,Wong AJ,Liu ED,Saab L,King AJ,Pae H,Ajayi MT,Anderson GR
A-11122 was used in Immunohistochemistry to reveal Lphn2 to be selectively expressed by defined cell types and essential for MEC-PrS circuit connectivity.
Tue Nov 23 00:00:00 EST 2021
The ubiquitin isopeptidase USP10 deubiquitinates LC3B to increase LC3B levels and autophagic activity.
The Journal of biological chemistry
Jia R,Bonifacino JS
A-11122 was used in Western Blot to identify mechanisms capable of counteracting the targeting of LC3B for proteasomal degradation by the ubiquitin-activating enzyme UBA6 and ubiquitin-conjugating enzyme/ubiquitin ligase BIRC6, which reduces autophagic activity under stress.
Fri Aug 27 00:00:00 EDT 2021
polished rice mediates ecdysone-dependent control of Drosophila embryonic organogenesis.
Genes to cells : devoted to molecular & cellular mechanisms
Taira Y,Wada H,Hayashi S,Kageyama Y
A-11122 was used in Immunohistochemistry-immunofluorescence to demonstrate that ecdysone and its downstream target pri play essential roles in tracheal development by modulating cell fate decision.
Sat May 01 00:00:00 EDT 2021
Single-cell visualization of mir-9a and Senseless co-expression during Drosophila melanogaster embryonic and larval peripheral nervous system development.
G3 (Bethesda, Md.)
Gallicchio L,Griffiths-Jones S,Ronshaugen M
A-11122 was used in Immunohistochemistry to show that the expression of mir-9a in SOP cells is rapidly lost as Senseless expression increases.
Mon Jan 18 00:00:00 EST 2021
Patterns of differentiation of renin lineage cells during nephrogenesis.
American journal of physiology. Renal physiology
Kessel F,Steglich A,Hickmann L,Lira Martinez R,Gerlach M,Sequeira-Lopez MLS,Gomez RA,Hugo CPM,Todorov VT
A-11122 was used in Immunohistochemistry-immunofluorescence to quantify different renal vascular and tubular cell types based on specific markers and assessed proliferation and de novo differentiation in the RLC population.
Wed Sep 01 00:00:00 EDT 2021
Population calcium responses of Purkinje cells in the oculomotor cerebellum driven by nonvisual input.
Journal of neurophysiology
Fanning AS,Shakhawat AM,Raymond JL
A-11122 was used in Immunohistochemistry-immunofluorescence to report that both visual and nonvisual inputs can elicit robust calcium responses in the Purkinje cells, suggesting that the instructive signals guiding oculomotor plasticity are multimodal.
Fri Oct 01 00:00:00 EDT 2021
Differential expression of striatal proteins in a mouse model of DOPA-responsive dystonia reveals shared mechanisms among dystonic disorders.
Molecular genetics and metabolism
Briscione MA,Dinasarapu AR,Bagchi P,Donsante Y,Roman KM,Downs AM,Fan X,Hoehner J,Jinnah HA,Hess EJ
A-11122 was used in Immunohistochemistry to study the differential expression of striatal proteins in a mouse model of DOPA-responsive dystonia.
Sun Aug 01 00:00:00 EDT 2021
Parallel ascending spinal pathways for affective touch and pain.
Nature
Choi S,Hachisuka J,Brett MA,Magee AR,Omori Y,Iqbal NU,Zhang D,DeLisle MM,Wolfson RL,Bai L,Santiago C,Gong S,Goulding M,Heintz N,Koerber HR,Ross SE,Ginty DD
A-11122 was used in Immunohistochemistry to show that two populations of projection neurons that express the structurally related G-protein-coupled receptors (GPCRs) TACR1 and GPR83 form parallel ascending circuit modules that cooperate to convey thermal, tactile and noxious cutaneous signals from the spinal cord to the lateral parabrachial nucleus of the pons.
Sun Nov 01 00:00:00 EDT 2020
Epigenetic gene silencing by heterochromatin primes fungal resistance.
Nature
Torres-Garcia S,Yaseen I,Shukla M,Audergon PNCB,White SA,Pidoux AL,Allshire RC
A-11122 was used in Chromatin immunoprecipitation to reveal that epigenetic processes promote phenotypic plasticity, letting wild-type cells adapt to unfavourable environments without genetic alteration.
Tue Sep 01 00:00:00 EDT 2020
PIK3CA variants selectively initiate brain hyperactivity during gliomagenesis.
Nature
Yu K,Lin CJ,Hatcher A,Lozzi B,Kong K,Huang-Hobbs E,Cheng YT,Beechar VB,Zhu W,Zhang Y,Chen F,Mills GB,Mohila CA,Creighton CJ,Noebels JL,Scott KL,Deneen B
A-11122 was used in Immunohistochemistry to develop a high-throughput in vivo screening platform and discover several driver variants of PIK3CA, using a native mouse model of glioblastoma.
Sat Feb 01 00:00:00 EST 2020
TDP-43 Triggers Mitochondrial DNA Release via mPTP to Activate cGAS/STING in ALS.
Cell
Yu CH,Davidson S,Harapas CR,Hilton JB,Mlodzianoski MJ,Laohamonthonkul P,Louis C,Low RRJ,Moecking J,De Nardo D,Balka KR,Calleja DJ,Moghaddas F,Ni E,McLean CA,Samson AL,Tyebji S,Tonkin CJ,Bye CR,Turner BJ,Pepin G,Gantier MP,Rogers KL,McArthur K,Crouch PJ,Masters SL
A-11122 was used in Immunocytochemistry-immunoflourescence to show that amyotrophic lateral sclerosis (ALS), associated neuroinflammatory cytokine profile is driven by the cytoplasmic DNA sensor cyclic guanosine monophosphate (GMP)-AMP synthase (cGAS) when TDP-43 invades mitochondria and releases DNA via the permeability transition pore.
Thu Oct 29 00:00:00 EDT 2020
Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.
Nature methods
Zhang Y,Schroeder LK,Lessard MD,Kidd P,Chung J,Song Y,Benedetti L,Li Y,Ries J,Grimm JB,Lavis LD,De Camilli P,Rothman JE,Baddeley D,Bewersdorf J
A-11122 was used in Western Blot to resolve the highly convoluted Golgi apparatus and the close contacts between the endoplasmic reticulum and the plasma membrane with three-dimensional fluorescence images.
Sat Feb 01 00:00:00 EST 2020
Unbiased Proteomic Profiling Uncovers a Targetable GNAS/PKA/PP2A Axis in Small Cell Lung Cancer Stem Cells.
Cancer cell
Coles GL,Cristea S,Webber JT,Levin RS,Moss SM,He A,Sangodkar J,Hwang YC,Arand J,Drainas AP,Mooney NA,Demeter J,Spradlin JN,Mauch B,Le V,Shue YT,Ko JH,Lee MC,Kong C,Nomura DK,Ohlmeyer M,Swaney DL,Krogan NJ,Jackson PK,Narla G,Gordan JD,Shokat KM,Sage J
A-11122 was used in Western Blot to identify protein kinase A (PKA) as an active kinase in small cell lung cancer (SCLC).
Mon Jul 13 00:00:00 EDT 2020
PTEN Loss Mediates Clinical Cross-Resistance to CDK4/6 and PI3Kα Inhibitors in Breast Cancer.
Cancer discovery
Costa C,Wang Y,Ly A,Hosono Y,Murchie E,Walmsley CS,Huynh T,Healy C,Peterson R,Yanase S,Jakubik CT,Henderson LE,Damon LJ,Timonina D,Sanidas I,Pinto CJ,Mino-Kenudson M,Stone JR,Dyson NJ,Ellisen LW,Bardia A,Ebi H,Benes CH,Engelman JA,Juric D
A-11122 was used in Immunocytochemistry to reveal that some resistant tumors acquired RB loss, whereas other tumors lost PTEN expression at the time of progression.
Wed Jan 01 00:00:00 EST 2020
Oncogenic Mutations in Armadillo Repeats 5 and 6 of β-Catenin Reduce Binding to APC, Increasing Signaling and Transcription of Target Genes.
Gastroenterology
Liu P,Liang B,Liu M,Lebbink JHG,Li S,Qian M,Lavrijsen M,Peppelenbosch MP,Chen X,Smits R
A-11122 was used in Western Blotting to investigate the mechanisms by which these substitutions increase signaling and the effects on liver carcinogenesis in mice.
Sun Mar 01 00:00:00 EST 2020
Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.
Cell metabolism
Mukherjee C,Kling T,Russo B,Miebach K,Kess E,Schifferer M,Pedro LD,Weikert U,Fard MK,Kannaiyan N,Rossner M,Aicher ML,Goebbels S,Nave KA,Krämer-Albers EM,Schneider A,Simons M
A-11122 was used in Western Blot to identify glial molecules providing vital functions to neurons.
Tue Aug 04 00:00:00 EDT 2020
Associative and plastic thalamic signaling to the lateral amygdala controls fear behavior.
Nature neuroscience
Barsy B,Kocsis K,Magyar A,Babiczky Á,Szabó M,Veres JM,Hillier D,Ulbert I,Yizhar O,Mátyás F
A-11122 was used in Immunohistochemistry to demonstrate that Calr+LT neurons provide integrated CS-US representations to the LA that support the formation of aversive memories.
Fri May 01 00:00:00 EDT 2020
Posterior amygdala regulates sexual and aggressive behaviors in male mice.
Nature neuroscience
Yamaguchi T,Wei D,Song SC,Lim B,Tritsch NX,Lin D
A-11122 was used in Immunohistochemistry-immunofluorescence to identify estrogen receptor alpha (Esr1)-expressing cells in the posterior amygdala (PA) as a main source of excitatory inputs to the hypothalamus and key mediators for mating and fighting in male mice.
Tue Sep 01 00:00:00 EDT 2020
Anatomic resolution of neurotransmitter-specific projections to the VTA reveals diversity of GABAergic inputs.
Nature neuroscience
Soden ME,Chung AS,Cuevas B,Resnick JM,Awatramani R,Zweifel LS
A-11122 was used in Immunohistochemistry to map the major neurotransmitter projections to the ventral tegmental area through cell-type-specific retrograde and anterograde tracing.
Sat Aug 01 00:00:00 EDT 2020
Dynamin regulates the dynamics and mechanical strength of the actin cytoskeleton as a multifilament actin-bundling protein.
Nature cell biology
Zhang R,Lee DM,Jimah JR,Gerassimov N,Yang C,Kim S,Luvsanjav D,Winkelman J,Mettlen M,Abrams ME,Kalia R,Keene P,Pandey P,Ravaux B,Kim JH,Ditlev JA,Zhang G,Rosen MK,Frost A,Alto NM,Gardel M,Schmid SL,Svitkina TM,Hinshaw JE,Chen EH
A-11122 was used in Immunocytochemistry to reveal a function of dynamin in propelling invasive membrane protrusions during myoblast fusion in vivo.
Mon Jun 01 00:00:00 EDT 2020
Systematic Discovery of Short Linear Motifs Decodes Calcineurin Phosphatase Signaling.
Molecular cell
Wigington CP,Roy J,Damle NP,Yadav VK,Blikstad C,Resch E,Wong CJ,Mackay DR,Wang JT,Krystkowiak I,Bradburn DA,Tsekitsidou E,Hong SH,Kaderali MA,Xu SL,Stearns T,Gingras AC,Ullman KS,Ivarsson Y,Davey NE,Cyert MS
A-11122 was used in Immunocytochemistry to elucidate the regulatory network of calcineurin (CN)/PP2B, the Ca2+-activated phosphatase that recognizes LxVP and PxIxIT motifs.
Thu Jul 16 00:00:00 EDT 2020
Context-Dependent Gene Regulation by Homeodomain Transcription Factor Complexes Revealed by Shape-Readout Deficient Proteins.
Molecular cell
Kribelbauer JF,Loker RE,Feng S,Rastogi C,Abe N,Rube HT,Bussemaker HJ,Mann RS
A-11122 was used in Immunohistochemistry to demonstrate that high-throughput in vitro DNA binding assays coupled with unbiased computational analysis provide unprecedented insight into how different DNA sequences select distinct compositions and configurations of homeodomain TF complexes.
Thu Apr 02 00:00:00 EDT 2020
The fungal CCAAT-binding complex and HapX display highly variable but evolutionary conserved synergetic promoter-specific DNA recognition.
Nucleic acids research
Furukawa T,Scheven MT,Misslinger M,Zhao C,Hoefgen S,Gsaller F,Lau J,Jöchl C,Donaldson I,Valiante V,Brakhage AA,Bromley MJ,Haas H,Hortschansky P
A-11122 was used in Chromatin immunoprecipitation to show that CBC:HapX interaction is absolutely required for all known functions of HapX.
Fri Apr 17 00:00:00 EDT 2020
Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species.
Nucleic acids research
He X,Chen W,Liu Z,Yu G,Chen Y,Cai YJ,Sun L,Xu W,Zhong L,Gao C,Chen J,Zhang M,Yang S,Yao Y,Zhang Z,Ma F,Zhang CC,Lu HP,Yu B,Cheng TL,Qiu J,Sheng Q,Zhou HM,Lv ZR,Yan J,Zhou Y,Qiu Z,Cui Z,Zhang X,Meng A,Sun Q,Yang Y
A-11122 was used in Immunohistochemistry-immunofluorescence to develop an efficient and low-risk gene editing method.
Thu Jun 04 00:00:00 EDT 2020
The FMRP-MOV10 complex: a translational regulatory switch modulated by G-Quadruplexes.
Nucleic acids research
Kenny PJ,Kim M,Skariah G,Nielsen J,Lannom MC,Ceman S
A-11122 was used in Western Blot to characterize the FMRP-containing mRNP that regulates translation of bound mRNAs at the molecular level.
Fri Jan 24 00:00:00 EST 2020
Transcription factor Tcf4 is the preferred heterodimerization partner for Olig2 in oligodendrocytes and required for differentiation.
Nucleic acids research
Wedel M,Fröb F,Elsesser O,Wittmann MT,Lie DC,Reis A,Wegner M
A-11122 was used in Immunocytochemistry to point to the possibility of an oligodendroglial contribution to these disorders.
Thu May 21 00:00:00 EDT 2020
CaMKII Measures the Passage of Time to Coordinate Behavior and Motivational State.
Neuron
Thornquist SC,Langer K,Zhang SX,Rogulja D,Crickmore MA
A-11122 was used in Immunohistochemistry-immunofluorescence to discover whether the activation and gradual relaxation of calcium-independent CaMKII measure a 6-min time window to coordinate two male-specific events during Drosophila mating: sperm transfer and a simultaneous decrease in motivation.
Wed Jan 22 00:00:00 EST 2020
Encoding of 3D Head Orienting Movements in the Primary Visual Cortex.
Neuron
Guitchounts G,Masís J,Wolff SBE,Cox D
A-11122 was used in Immunohistochemistry-immunofluorescence to show that 3D head-orienting movements (HOMs) modulate primary visual cortex (V1) activity in a direction-specific manner that also depends on light.
Wed Nov 11 00:00:00 EST 2020
Neurons that Function within an Integrator to Promote a Persistent Behavioral State in Drosophila.
Neuron
Jung Y,Kennedy A,Chiu H,Mohammad F,Claridge-Chang A,Anderson DJ
A-11122 was used in Immunohistochemistry to identify pCd neurons as persistently active, indirect P1 targets that are required for P1-evoked persistent courtship and aggression.
Wed Jan 22 00:00:00 EST 2020
Selective Mesoaccumbal Pathway Inactivation Affects Motivation but Not Reinforcement-Based Learning in Macaques.
Neuron
Vancraeyenest P,Arsenault JT,Li X,Zhu Q,Kobayashi K,Isa K,Isa T,Vanduffel W
A-11122 was used in Immunohistochemistry to demonstrate that selective, unidirectional, and reversible blockage of the primarily dopaminergic mesoaccumbal circuit in monkeys increased network-level functional connectivity, especially in fronto-temporal cortex.
Wed Nov 11 00:00:00 EST 2020
LRRTM4: A Novel Regulator of Presynaptic Inhibition and Ribbon Synapse Arrangements of Retinal Bipolar Cells.
Neuron
Sinha R,Siddiqui TJ,Padmanabhan N,Wallin J,Zhang C,Karimi B,Rieke F,Craig AM,Wong RO,Hoon M
A-11122 was used in Western Blotting to find that LRRTM4 is enriched at GABAergic synapses on axon terminals of rod bipolar cells (RBCs).
Wed Mar 18 00:00:00 EDT 2020
Synergy of Distinct Dopamine Projection Populations in Behavioral Reinforcement.
Neuron
Heymann G,Jo YS,Reichard KL,McFarland N,Chavkin C,Palmiter RD,Soden ME,Zweifel LS
A-11122 was used in Immunohistochemistry to identify functionally distinct dopaminergic neurons in the ventral tegmental area involved in motivation and reward.
Wed Mar 04 00:00:00 EST 2020
Tissue-resident memory T cell reactivation by diverse antigen-presenting cells imparts distinct functional responses.
The Journal of experimental medicine
Low JS,Farsakoglu Y,Amezcua Vesely MC,Sefik E,Kelly JB,Harman CCD,Jackson R,Shyer JA,Jiang X,Cauley LS,Flavell RA,Kaech SM
A-11122 was used in Immunocytochemistry to demonstrate that lung CD8+ TRM cells are reactivated more quickly, yet less efficiently, than their counterparts in the draining LNs (TLN cells).
Mon Aug 03 00:00:00 EDT 2020
Single-cell profiling reveals an endothelium-mediated immunomodulatory pathway in the eye choroid.
The Journal of experimental medicine
Lehmann GL,Hanke-Gogokhia C,Hu Y,Bareja R,Salfati Z,Ginsberg M,Nolan DJ,Mendez-Huergo SP,Dalotto-Moreno T,Wojcinski A,Ochoa F,Zeng S,Cerliani JP,Panagis L,Zager PJ,Mullins RF,Ogura S,Lutty GA,Bang J,Zippin JH,Romano C,Rabinovich GA,Elemento O,Joyner AL,Rafii S,Rodriguez-Boulan E,Benedicto I
A-11122 was used in Immunocytochemistry to open new avenues for the study and treatment of retinal vascular diseases and choroid-related inflammatory blinding disorders.
Mon Jun 01 00:00:00 EDT 2020
The colonic macrophage transcription factor RBP-J orchestrates intestinal immunity against bacterial pathogens.
The Journal of experimental medicine
Kang L,Zhang X,Ji L,Kou T,Smith SM,Zhao B,Guo X,Pineda-Torra I,Wu L,Hu X
A-11122 was used in Immunocytochemistry to demonstrate that colonic macrophage-intrinsic RBP-J dynamically orchestrates intestinal immunity against pathogen infections by interfacing with key immune cells of T and innate lymphoid cell lineages.
Mon Apr 06 00:00:00 EDT 2020
ZPR1 prevents R-loop accumulation, upregulates SMN2 expression and rescues spinal muscular atrophy.
Brain : a journal of neurology
Kannan A,Jiang X,He L,Ahmad S,Gangwani L
A-11122 was used in Western Blot to conclude that ZPR1 represents a protective modifier and a therapeutic target for developing a new method for the treatment of SMA.
Wed Jan 01 00:00:00 EST 2020
Global CNS correction in a large brain model of human alpha-mannosidosis by intravascular gene therapy.
Brain : a journal of neurology
Yoon SY,Hunter JE,Chawla S,Clarke DL,Molony C,O'Donnell PA,Bagel JH,Kumar M,Poptani H,Vite CH,Wolfe JH
A-11122 was used in Immunohistochemistry to study the potential for therapeutic application of the hu.32 serotype in a gyrencephalic brain of larger mammals.
Wed Jul 01 00:00:00 EDT 2020
Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration.
The EMBO journal
Weinert S,Gimber N,Deuschel D,Stuhlmann T,Puchkov D,Farsi Z,Ludwig CF,Novarino G,López-Cayuqueo KI,Planells-Cases R,Jentsch TJ
A-11122 was used in Immunohistochemistry to generate Clcn3unc/unc mice in which ClC-3 is converted into a Cl- channel.
Mon May 04 00:00:00 EDT 2020
RNA localization and co-translational interactions control RAB13 GTPase function and cell migration.
The EMBO journal
Moissoglu K,Stueland M,Gasparski AN,Wang T,Jenkins LM,Hastings ML,Mili S
A-11122 was used in Western Blot, Proximity Ligation Assay (PLA) to investigate the functional role of RNA localization at cellular protrusions of migrating mesenchymal cells, using as a model the RAB13 RNA, which encodes a GTPase important for vesicle-mediated membrane trafficking.
Mon Nov 02 00:00:00 EST 2020
RNA localization and co-translational interactions control RAB13 GTPase function and cell migration.
The EMBO journal
Moissoglu K,Stueland M,Gasparski AN,Wang T,Jenkins LM,Hastings ML,Mili S
A-11122 was used in Western Blot, Proximity Ligation Assay (PLA) to investigate the functional role of RNA localization at cellular protrusions of migrating mesenchymal cells, using as a model the RAB13 RNA, which encodes a GTPase important for vesicle-mediated membrane trafficking.
Mon Nov 02 00:00:00 EST 2020
Structural basis of semaphorin-plexin cis interaction.
The EMBO journal
Rozbesky D,Verhagen MG,Karia D,Nagy GN,Alvarez L,Robinson RA,Harlos K,Padilla-Parra S,Pasterkamp RJ,Jones EY
A-11122 was used in Immunocytochemistry to study the structural interaction and dynamics between the semaphorin-plexin cis interaction.
Wed Jul 01 00:00:00 EDT 2020
Distinct retrograde microtubule motor sets drive early and late endosome transport.
The EMBO journal
Villari G,Enrico Bena C,Del Giudice M,Gioelli N,Sandri C,Camillo C,Fiorio Pla A,Bosia C,Serini G
A-11122 was used in Western Blot, Immunoprecipitation to provide evidence that distinct minus end-directed MT motor systems drive the differential transport and subcellular distribution of EEs and LEs in mammalian cells.
Tue Dec 15 00:00:00 EST 2020
Distinct retrograde microtubule motor sets drive early and late endosome transport.
The EMBO journal
Villari G,Enrico Bena C,Del Giudice M,Gioelli N,Sandri C,Camillo C,Fiorio Pla A,Bosia C,Serini G
A-11122 was used in Western Blot, Immunoprecipitation to provide evidence that distinct minus end-directed MT motor systems drive the differential transport and subcellular distribution of EEs and LEs in mammalian cells.
Tue Dec 15 00:00:00 EST 2020
A microtubule polymerase is required for microtubule orientation and dendrite pruning in Drosophila.
The EMBO journal
Tang Q,Rui M,Bu S,Wang Y,Chew LY,Yu F
A-11122 was used in Western Blotting to demonstrate that the MT polymerase Msps is required to form dendritic minus-end-out MTs and thereby promotes dendrite pruning in Drosophila sensory neurons.
Mon May 18 00:00:00 EDT 2020
Glycolysis regulates Hedgehog signalling via the plasma membrane potential.
The EMBO journal
Spannl S,Buhl T,Nellas I,Zeidan SA,Iyer KV,Khaliullina H,Schultz C,Nadler A,Dye NA,Eaton S
A-11122 was used in Immunocytochemistry-Immunofluorescence to show that reducing glycolysis both lowers steady-state levels of ATP and stabilizes Smoothened (Smo), the 7-pass transmembrane protein that transduces the Hh signal.
Mon Nov 02 00:00:00 EST 2020
Tollip coordinates Parkin-dependent trafficking of mitochondrial-derived vesicles.
The EMBO journal
Ryan TA,Phillips EO,Collier CL,Jb Robinson A,Routledge D,Wood RE,Assar EA,Tumbarello DA
A-11122 was used in Immunocytochemistry-immunoflourescence to investigate the role of the endosomal adaptor Tollip during the mitochondrial stress response and identify its interaction and colocalisation with the Parkinson's disease-associated E3 ubiquitin ligase Parkin.
Tue Jun 02 00:00:00 EDT 2020
Tollip coordinates Parkin-dependent trafficking of mitochondrial-derived vesicles.
The EMBO journal
Ryan TA,Phillips EO,Collier CL,Jb Robinson A,Routledge D,Wood RE,Assar EA,Tumbarello DA
A-11122 was used in Immunocytochemistry-immunoflourescence to investigate the role of the endosomal adaptor Tollip during the mitochondrial stress response and identify its interaction and colocalisation with the Parkinson's disease-associated E3 ubiquitin ligase Parkin.
Tue Jun 02 00:00:00 EDT 2020
Histone H2A phosphorylation recruits topoisomerase IIα to centromeres to safeguard genomic stability.
The EMBO journal
Zhang M,Liang C,Chen Q,Yan H,Xu J,Zhao H,Yuan X,Liu J,Lin S,Lu W,Wang F
A-11122 was used in Immunocytochemistry to reveal a fundamental role for histone phosphorylation in resolving centromere DNA entanglements and safeguarding genomic stability during mitosis.
Mon Feb 03 00:00:00 EST 2020
Single-Cell Analysis of Human Retina Identifies Evolutionarily Conserved and Species-Specific Mechanisms Controlling Development.
Developmental cell
Lu Y,Shiau F,Yi W,Lu S,Wu Q,Pearson JD,Kallman A,Zhong S,Hoang T,Zuo Z,Zhao F,Zhang M,Tsai N,Zhuo Y,He S,Zhang J,Stein-O'Brien GL,Sherman TD,Duan X,Fertig EJ,Goff LA,Zack DJ,Handa JT,Xue T,Bremner R,Blackshaw S,Wang X,Clark BS
A-11122 was used in Immunohistochemistry to provide a roadmap to future studies of human retinal development and may help guide the design of cell-based therapies for treating retinal dystrophies.
Mon May 18 00:00:00 EDT 2020
AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis.
Developmental cell
Marneros AG
A-11122 was used in Immunohistochemistry to show that the transcription factor AP-2β is required for the differentiation of distal tubule precursors into early stage distal convoluted tubules during nephrogenesis.
Mon Aug 10 00:00:00 EDT 2020
ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway.
Autophagy
Deng Z,Lim J,Wang Q,Purtell K,Wu S,Palomo GM,Tan H,Manfredi G,Zhao Y,Peng J,Hu B,Chen S,Yue Z
A-11122 was used in Immunocytochemistry-immunoflourescence to show that amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD)-linked mutations of TBK1 and SQSTM1 disrupt selective autophagy and cause neurotoxicity.
Fri May 01 00:00:00 EDT 2020
Dysregulation of TFEB contributes to manganese-induced autophagic failure and mitochondrial dysfunction in astrocytes.
Autophagy
Zhang Z,Yan J,Bowman AB,Bryan MR,Singh R,Aschner M
A-11122 was used in Western Blotting to indicate that the dysregulation of TFEB (transcription factor EB) is involved in manganese-induced autotrophic failure.
Sat Aug 01 00:00:00 EDT 2020
mGreenLantern: a bright monomeric fluorescent protein with rapid expression and cell filling properties for neuronal imaging.
Proceedings of the National Academy of Sciences of the United States of America
Campbell BC,Nabel EM,Murdock MH,Lao-Peregrin C,Tsoulfas P,Blackmore MG,Lee FS,Liston C,Morishita H,Petsko GA
A-11122 was used in Immunocytochemistry to test hypothesis that EGFP's low solubility in mammalian systems must limit the total fluorescence output of whole cells, and that improving folding efficiency could therefore translate into greater brightness of expressing neurons.
Tue Dec 01 00:00:00 EST 2020
Neddylation is critical to cortical development by regulating Wnt/β-catenin signaling.
Proceedings of the National Academy of Sciences of the United States of America
Zhang L,Jing H,Li H,Chen W,Luo B,Zhang H,Dong Z,Li L,Su H,Xiong WC,Mei L
A-11122 was used in Immunohistochemistry to provide evidence for an indispensable role of neddylation, a ubiquitylation-like protein modification, in inhibiting Wnt/β-catenin signaling.
Tue Oct 20 00:00:00 EDT 2020
Functionally distinct Purkinje cell types show temporal precision in encoding locomotion.
Proceedings of the National Academy of Sciences of the United States of America
Chang W,Pedroni A,Hohendorf V,Giacomello S,Hibi M,Köster RW,Ampatzis K
A-11122 was used in Immunohistochemistry to show an undiscovered heterogeneity of adult zebrafish Purkinje cells, revealing the existence of anatomically and functionally distinct cell types.
Tue Jul 21 00:00:00 EDT 2020
Neuroadaptations in the dorsal hippocampus underlie cocaine seeking during prolonged abstinence.
Proceedings of the National Academy of Sciences of the United States of America
Werner CT,Mitra S,Auerbach BD,Wang ZJ,Martin JA,Stewart AF,Gobira PH,Iida M,An C,Cobb MM,Caccamise A,Salvi RJ,Neve RL,Gancarz AM,Dietz DM
A-11122 was used in Immunocytochemistry to demonstrate a role for the DH in relapse behavior following prolonged abstinence from cocaine self-administration.
Tue Oct 20 00:00:00 EDT 2020
Dimer interaction in the Hv1 proton channel.
Proceedings of the National Academy of Sciences of the United States of America
Mony L,Stroebel D,Isacoff EY
A-11122 was used in Western Blotting to investigate the gating mechanisms and the importance of dimer interactions within the gating of the Hv1 channel.
Tue Aug 25 00:00:00 EDT 2020
CRL5-dependent regulation of the small GTPases ARL4C and ARF6 controls hippocampal morphogenesis.
Proceedings of the National Academy of Sciences of the United States of America
Han JS,Hino K,Li W,Reyes RV,Canales CP,Miltner AM,Haddadi Y,Sun J,Chen CY,La Torre A,Simó S
A-11122 was used in Immunohistochemistry to investigate CRL5 as a key regulator of hippocampal development and uncovered ARL4C, CYTH1, and ARF6 as CRL5-regulated signaling effectors that control pyramidal neuron migration and dendritogenesis.
Tue Sep 15 00:00:00 EDT 2020
Retinal inputs signal astrocytes to recruit interneurons into visual thalamus.
Proceedings of the National Academy of Sciences of the United States of America
Su J,Charalambakis NE,Sabbagh U,Somaiya RD,Monavarfeshani A,Guido W,Fox MA
A-11122 was used in Immunohistochemistry to show that retinal inputs signal through astrocytes to direct the long-range recruitment of interneurons into the visual thalamus.
Tue Feb 04 00:00:00 EST 2020
The Chlamydia effector CT622/TaiP targets a nonautophagy related function of ATG16L1.
Proceedings of the National Academy of Sciences of the United States of America
Hamaoui D,Cossé MM,Mohan J,Lystad AH,Wollert T,Subtil A
A-11122 was used in Immunoprecipitation to show that the host autophagy-related protein 16-1 (ATG16L1) restricts inclusion growth and that this effect is counteracted by the secretion of the bacterial effector CT622/TaiP (translocated ATG16L1 interacting protein).
Tue Oct 27 00:00:00 EDT 2020
CRK2 and C-terminal Phosphorylation of NADPH Oxidase RBOHD Regulate Reactive Oxygen Species Production in Arabidopsis.
The Plant cell
Kimura S,Hunter K,Vaahtera L,Tran HC,Citterico M,Vaattovaara A,Rokka A,Stolze SC,Harzen A,Meißner L,Wilkens MMT,Hamann T,Toyota M,Nakagami H,Wrzaczek M
A-11122 was used in Immunoprecipitation to determine that reactive oxygen species production in Arabidopsis is regulated by CRK2 and the phosphorylation of the C-terminal region of NADPH oxidase.
Wed Apr 01 00:00:00 EDT 2020
Coupling of terminal differentiation deficit with neurodegenerative pathology in Vps35-deficient pyramidal neurons.
Cell death and differentiation
Tang FL,Zhao L,Zhao Y,Sun D,Zhu XJ,Mei L,Xiong WC
A-11122 was used in Immunohistochemistry to provide evidence that Vps35 in embryonic neurons is necessary for axonal and dendritic terminal differentiation.
Wed Jul 01 00:00:00 EDT 2020
NCKAP1 Disruptive Variants Lead to a Neurodevelopmental Disorder with Core Features of Autism.
American journal of human genetics
Guo H,Zhang Q,Dai R,Yu B,Hoekzema K,Tan J,Tan S,Jia X,Chung WK,Hernan R,Alkuraya FS,Alsulaiman A,Al-Muhaizea MA,Lesca G,Pons L,Labalme A,Laux L,Bryant E,Brown NJ,Savva E,Ayres S,Eratne D,Peeters H,Bilan F,Letienne-Cejudo L,Gilbert-Dussardier B,Ruiz-Arana IL,Merlini JM,Boizot A,Bartoloni L,Santoni F,Karlowicz D,McDonald M,Wu H,Hu Z,Chen G,Ou J,Brasch-Andersen C,Fagerberg CR,Dreyer I,Chun-Hui Tsai A,Slegesky V,McGee RB,Daniels B,Sellars EA,Carpenter LA,Schaefer B,Sacoto MJG,Begtrup A,Schnur RE,Punj S,Wentzensen IM,Rhodes L,Pan Q,Bernier RA,Chen C,Eichler EE,Xia K
A-11122 was used in Immunohistochemistry to show that NCKAP1 is broadly and highly expressed in both prenatal and postnatal periods and demostrate enriched expression in excitatory neurons and radial glias but depleted expression in inhibitory neurons.
Thu Nov 05 00:00:00 EST 2020
Ovipositor Extrusion Promotes the Transition from Courtship to Copulation and Signals Female Acceptance in Drosophila melanogaster.
Current biology : CB
Mezzera C,Brotas M,Gaspar M,Pavlou HJ,Goodwin SF,Vasconcelos ML
A-11122 was used in Immunohistochemistry to demonstrate the role of oviposter extrusion during Drosophila melanogaster copulation.
Mon Oct 05 00:00:00 EDT 2020
A Feedforward Circuit Regulates Action Selection of Pre-mating Courtship Behavior in Female Drosophila.
Current biology : CB
Ishimoto H,Kamikouchi A
A-11122 was used in Immunohistochemistry to elucidate the molecular and neural mechanisms regulating the behavioral selection process of the pre-mating female.
Mon Feb 03 00:00:00 EST 2020
Integrins Modulate Extracellular Matrix Organization to Control Cell Signaling during Hematopoiesis.
Current biology : CB
Khadilkar RJ,Ho KYL,Venkatesh B,Tanentzapf G
A-11122 was used in Immunohistochemistry to provide mechanistic insight into how integrins can shape the signaling environment around hematopoietic progenitors.
Mon Sep 07 00:00:00 EDT 2020
Parallel Mechanosensory Pathways Direct Oviposition Decision-Making in Drosophila.
Current biology : CB
Zhang L,Yu J,Guo X,Wei J,Liu T,Zhang W
A-11122 was used in Immunohistochemistry to uncover drosophila mechanisms of detection of substrate quality for oviposition.
Mon Aug 17 00:00:00 EDT 2020
Interclass GPCR heteromerization affects localization and trafficking.
Science signaling
Toneatti R,Shin JM,Shah UH,Mayer CR,Saunders JM,Fribourg M,Arsenovic PT,Janssen WG,Sealfon SC,López-Giménez JF,Benson DL,Conway DE,González-Maeso J
A-11122 was used in Immunocytochemistry-immunoflourescence to show that the class A serotonin 5-HT2A receptors (5-HT2ARs) affected the localization and trafficking of class C metabotropic glutamate receptor 2 (mGluR2) through a mechanism that required their assembly as heteromers in mammalian cells.
Tue Oct 20 00:00:00 EDT 2020
Centromere-localized Aurora B kinase is required for the fidelity of chromosome segregation.
The Journal of cell biology
Liang C,Zhang Z,Chen Q,Yan H,Zhang M,Zhou L,Xu J,Lu W,Wang F
A-11122 was used in Western Blotting to provide insights into how centromeric Aurora B regulates SAC and kinetochore attachment to microtubules to ensure error-free chromosome segragation.
Mon Feb 03 00:00:00 EST 2020
A pair of E3 ubiquitin ligases compete to regulate filopodial dynamics and axon guidance.
The Journal of cell biology
Boyer NP,McCormick LE,Menon S,Urbina FL,Gupton SL
A-11122 was used in Western Blotting to show that the TRIM9-related protein TRIM67 outcompetes TRIM9 for interacting with VASP and antagonizes TRIM9-dependent VASP ubiquitination.
Mon Jan 06 00:00:00 EST 2020
centrocortin RNA localization to centrosomes is regulated by FMRP and facilitates error-free mitosis.
The Journal of cell biology
Ryder PV,Fang J,Lerit DA
A-11122 was used in Immunoprecipitation to investigate the regulation of mRNA localization to centrosomes in the rapidly cycling Drosophila melanogaster embryo.
Mon Dec 07 00:00:00 EST 2020
Exercise-dependent increases in protein synthesis are accompanied by chromatin modifications and increased MRTF-SRF signalling.
Acta physiologica (Oxford, England)
Solagna F,Nogara L,Dyar KA,Greulich F,Mir AA,Türk C,Bock T,Geremia A,Baraldo M,Sartori R,Farup J,Uhlenhaut H,Vissing K,Krüger M,Blaauw B
A-11122 was used in Western Blotting to identify new signalling pathways important for muscle remodelling after exercise.
Tue Sep 01 00:00:00 EDT 2020
Transcriptomic and functional studies reveal undermined chemotactic and angiostimulatory properties of aged microglia during stroke recovery.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
Jiang L,Mu H,Xu F,Xie D,Su W,Xu J,Sun Z,Liu S,Luo J,Shi Y,Leak RK,Wechsler LR,Chen J,Hu X
A-11122 was used in Immunohistochemistry to provide a new resource to understand the mechanisms underlying microglial alterations in the aged brain milieu and sheds light on new strategies to improve microglial functions in aged stroke victims.
Tue Dec 01 00:00:00 EST 2020
A genetic system for tissue-specific inhibition of cell proliferation.
Development (Cambridge, England)
Pu W,Han X,He L,Li Y,Huang X,Zhang M,Lv Z,Yu W,Wang QD,Cai D,Wang J,Sun R,Fei J,Ji Y,Nie Y,Zhou B
A-11122 was used in Immunohistochemistry to validate the use of ip21-GFP mice as a new genetic tool for cell lineage-specific inhibition of cell proliferation in vivo.
Mon Feb 17 00:00:00 EST 2020
Survival of Drosophila germline stem cells requires the chromatin-binding protein Barrier-to-autointegration factor.
Development (Cambridge, England)
Duan T,Kitzman SC,Geyer PK
A-11122 was used in Western Blotting to show that germ cell-specific BAF knockdown causes phenotypes that mirror emerin/otefin mutants.
Wed May 13 00:00:00 EDT 2020
Different modes of Notch activation and strength regulation in the spermathecal secretory lineage.
Development (Cambridge, England)
Shen W,Sun J
A-11122 was used in Immunocytochemistry to show that the Drosophila spermathecal lineage is an exciting model for probing the molecular mechanisms that modulate the Notch signaling pathway.
Fri Feb 07 00:00:00 EST 2020
Identification and requirements of enhancers that direct gene expression during zebrafish fin regeneration.
Development (Cambridge, England)
Thompson JD,Ou J,Lee N,Shin K,Cigliola V,Song L,Crawford GE,Kang J,Poss KD
A-11122 was used in Immunohistochemistry to identify candidate tissue regeneration enhancer elements (TREEs) important for zebrafish fin regeneration.
Thu Jul 30 00:00:00 EDT 2020
AMP-activated protein kinase regulates cytoplasmic dynein behavior and contributes to neuronal migration in the developing neocortex.
Development (Cambridge, England)
Naito Y,Asada N,Nguyen MD,Sanada K
A-11122 was used in Western Blotting to conclude that AMPK-regulated dynein function via Ser81 DIC phosphorylation is crucial for radial neuronal migration.
Wed Jul 15 00:00:00 EDT 2020
Proteolytic cleavage of Slit by the Tolkin protease converts an axon repulsion cue to an axon growth cue in vivo.
Development (Cambridge, England)
Kellermeyer R,Heydman LM,Gillis T,Mastick GS,Song M,Kidd T
A-11122 was used in Immunohistochemistry to report that the BMP-1/Tolloid family metalloprotease Tolkin (Tok) is responsible for Slit proteolysis in vivo and in vitro.
Thu Oct 29 00:00:00 EDT 2020
The kinesin motor Klp98A mediates apical to basal Wg transport.
Development (Cambridge, England)
Witte L,Linnemannstöns K,Schmidt K,Honemann-Capito M,Grawe F,Wodarz A,Gross JC
A-11122 was used in Immunocytochemistry to conclude that transcytosis-independent routes for Wg trafficking exist and demonstrate that Wg can be recycled apically via Rab4-recycling endosomes in the absence of Klp98A.
Fri Aug 14 00:00:00 EDT 2020
Ykt6-dependent endosomal recycling is required for Wnt secretion in the Drosophila wing epithelium.
Development (Cambridge, England)
Linnemannstöns K,Witte L,Karuna M P,Kittel JC,Danieli A,Müller D,Nitsch L,Honemann-Capito M,Grawe F,Wodarz A,Gross JC
A-11122 was used in Immunocytochemistry to investigate how Wnts are sorted at the level of endosomes by the versatile v-SNARE Ykt6.
Fri Aug 14 00:00:00 EDT 2020
Genetic developmental timing revealed by inter-species transplantations in fish.
Development (Cambridge, England)
Fuhrmann JF,Buono L,Adelmann L,Martinez-Morales JR,Centanin L
A-11122 was used in Immunohistochemistry to report that inter-species blastula transplantations result in the ectopic formation of a retina formed by donor cells - a module.
Mon Nov 23 00:00:00 EST 2020
Cortical distribution of GABAergic interneurons is determined by migration time and brain size.
Development (Cambridge, England)
Fazzari P,Mortimer N,Yabut O,Vogt D,Pla R
A-11122 was used in Immunohistochemistry to suggest that the embryonic expansion of the cortex contributes to the rostrocaudal distribution of CINs.
Wed Jul 22 00:00:00 EDT 2020
An autoregulatory switch in sex-specific phf7 transcription causes loss of sexual identity and tumors in the Drosophila female germline.
Development (Cambridge, England)
Smolko AE,Shapiro-Kulnane L,Salz HK
A-11122 was used in Immunocytochemistry to reveal that ectopic PHF7 in female germ cells leads to a loss of sexual identity and the promotion of a regulatory circuit that is beneficial for tumor initiation and progression.
Fri Sep 11 00:00:00 EDT 2020
The Drosophila NCAM homolog Fas2 signals independently of adhesion.
Development (Cambridge, England)
Neuert H,Deing P,Krukkert K,Naffin E,Steffes G,Risse B,Silies M,Klämbt C
A-11122 was used in Immunohistochemistry-immunofluorescence to study the function of single isoforms by conducting a comprehensive genetic analysis of F as2 We reveal the expression pattern of all major Fas2 isoforms, two of which are GPI anchored.
Wed Jan 22 00:00:00 EST 2020
Cardiac function modulates endocardial cell dynamics to shape the cardiac outflow tract.
Development (Cambridge, England)
Sidhwani P,Leerberg DM,Boezio GLM,Capasso TL,Yang H,Chi NC,Roman BL,Stainier DYR,Yelon D
A-11122 was used in Immunohistochemistry to indicate that cardiac function modulates OFT morphogenesis by triggering endocardial cell accumulation that induces OFT lumen expansion and shapes OFT dimensions.
Wed Jun 17 00:00:00 EDT 2020
Fgf8 genetic labeling reveals the early specification of vestibular hair cell type in mouse utricle.
Development (Cambridge, England)
Ratzan EM,Moon AM,Deans MR
A-11122 was used in Immunohistochemistry to elucidate the role of FGF8 signaling in vestibular hair cell differentiation.
Thu Nov 19 00:00:00 EST 2020
Dopamine Receptor Dop1R2 Stabilizes Appetitive Olfactory Memory through the Raf/MAPK Pathway in Drosophila.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Sun H,Nishioka T,Hiramatsu S,Kondo S,Amano M,Kaibuchi K,Ichinose T,Tanimoto H
A-11122 was used in Immunohistochemistry-immunofluorescence to investigate the downstream intracellular molecular signaling underlying memory stabilization in Drosophila.
Wed Apr 01 00:00:00 EDT 2020
Identification of Dopaminergic Neurons That Can Both Establish Associative Memory and Acutely Terminate Its Behavioral Expression.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Schleyer M,Weiglein A,Thoener J,Strauch M,Hartenstein V,Kantar Weigelt M,Schuller S,Saumweber T,Eichler K,Rohwedder A,Merhof D,Zlatic M,Thum AS,Gerber B
A-11122 was used in Immunohistochemistry to investigate the neuronal circuitry that allows larval Drosophila melanogaster of either sex to negotiate this exploration-exploitation transition.
Wed Jul 29 00:00:00 EDT 2020
Nitric Oxide Signaling Strengthens Inhibitory Synapses of Cerebellar Molecular Layer Interneurons through a GABARAP-Dependent Mechanism.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Larson EA,Accardi MV,Wang Y,D'Antoni M,Karimi B,Siddiqui TJ,Bowie D
A-11122 was used in Immunoprecipitation to show that activation of NMDARs trigger a NO-dependent pathway that strengthens inhibitory GABAergic synapses of cerebellar molecular layer interneurons.
Wed Apr 22 00:00:00 EDT 2020
Drosophila Voltage-Gated Sodium Channels Are Only Expressed in Active Neurons and Are Localized to Distal Axonal Initial Segment-like Domains.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Ravenscroft TA,Janssens J,Lee PT,Tepe B,Marcogliese PC,Makhzami S,Holmes TC,Aerts S,Bellen HJ
A-11122 was used in Western Blotting to determine the endogenous gene expression and subcellular localization of the sole NaV channel in both male and female Drosophila, para Despite being the only NaV channel in the fly, we show that only 23 1% of neurons in the embryonic and larval CNS express para, while in the adult CNS para is broadly expressed.
Wed Oct 14 00:00:00 EDT 2020
Mapping of Extrinsic Innervation of the Gastrointestinal Tract in the Mouse Embryo.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Niu X,Liu L,Wang T,Chuan X,Yu Q,Du M,Gu Y,Wang L
A-11122 was used in Immunohistochemistry to provide basic anatomic information to explore the mechanisms underlying the process of assembling neural circuitry in the gut-brain axis (GBA).
Wed Aug 26 00:00:00 EDT 2020
Accumulation of Neurofascin at Nodes of Ranvier Is Regulated by a Paranodal Switch.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Zhang Y,Yuen S,Peles E,Salzer JL
A-11122 was used in Western Blotting to examine the role of paranodal junctions in the accumulation of NF186, a key adhesion molecule of PNS and CNS nodes.
Wed Jul 22 00:00:00 EDT 2020
Myosin X Interaction with KIF13B, a Crucial Pathway for Netrin-1-Induced Axonal Development.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Yu HL,Peng Y,Zhao Y,Lan YS,Wang B,Zhao L,Sun D,Pan JX,Dong ZQ,Mei L,Ding YQ,Zhu XJ,Xiong WC
A-11122 was used in Immunoprecipitation to reveal Myo X-KIF13B as a critical pathway for Netrin-1-promoted axon initiation and branching/targeting.SIGNIFICANCE STATEMENT Netrin-1 increases Myosin X (Myo X) interaction with KIF13B, and thus promotes axonal delivery of Myo X and axon initiation and contralateral branching in developing cerebral neurons, revealing unrecognized functions and mechanisms underlying Netrin-1 regulation of axon development.
Wed Nov 25 00:00:00 EST 2020
The Mesolimbic Dopamine Activity Signatures of Relapse to Alcohol-Seeking.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Liu Y,Jean-Richard-Dit-Bressel P,Yau JO,Willing A,Prasad AA,Power JM,Killcross S,Clifford CWG,McNally GP
A-11122 was used in Immunohistochemistry to show how different forms of relapse to alcohol-seeking in male rats are assembled from activity across the VTA and the nucleus accumbens.
Wed Aug 12 00:00:00 EDT 2020
Phosphorylation of eIF2α Promotes Schwann Cell Differentiation and Myelination in CMT1B Mice with Activated UPR.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Scapin C,Ferri C,Pettinato E,Bianchi F,Del Carro U,Feltri ML,Kaufman RJ,Wrabetz L,D'Antonio M
A-11122 was used in Western Blotting to provide genetic evidence that eIF2α phosphorylation has a protective role in Charcot-Marie-Tooth type 1B Schwann cells by limiting ERK/c-Jun hyperactivation.
Wed Oct 14 00:00:00 EDT 2020
Environmental Light Is Required for Maintenance of Long-Term Memory in Drosophila.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Inami S,Sato S,Kondo S,Tanimoto H,Kitamoto T,Sakai T
A-11122 was used in Immunohistochemistry to demonstrate that universally available environmental light plays a critical role in LTM maintenance by activating the evolutionarily conserved memory modulator CREB in MBs via the Pdf signaling pathway.
Wed Feb 12 00:00:00 EST 2020
Paracrine Role for Somatostatin Interneurons in the Assembly of Perisomatic Inhibitory Synapses.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Su J,Basso D,Iyer S,Su K,Wei J,Fox MA
A-11122 was used in Immunohistochemistry to identify an extracellular matrix protein that is released by these early-born SST+ neurons to orchestrate inhibitory circuit formation in the developing cerebral cortex.
Wed Sep 23 00:00:00 EDT 2020
Identification and Characterization of the Heat-Induced Plastidial Stress Granules Reveal New Insight Into Arabidopsis Stress Response.
Frontiers in plant science
Chodasiewicz M,Sokolowska EM,Nelson-Dittrich AC,Masiuk A,Beltran JCM,Nelson ADL,Skirycz A
A-11122 was used in Affinity Purification to study the stress granule formation response in the model plant Arabidopsis thaliana.
Tue Nov 24 00:00:00 EST 2020
Reverse Genetics Reveals a Role of Rotavirus VP3 Phosphodiesterase Activity in Inhibiting RNase L Signaling and Contributing to Intestinal Viral Replication In Vivo.
Journal of virology
Song Y,Feng N,Sanchez-Tacuba L,Yasukawa LL,Ren L,Silverman RH,Ding S,Greenberg HB
A-11122 was used in Western Blotting to show viral replication within the intestine can be enhanced by inhibition of RNase L signaling by rotavirus VP3 phosphodiesterase.
Thu Apr 16 00:00:00 EDT 2020
Structure-Guided Mutagenesis Alters Deubiquitinating Activity and Attenuates Pathogenesis of a Murine Coronavirus.
Journal of virology
Deng X,Chen Y,Mielech AM,Hackbart M,Kesely KR,Mettelman RC,O'Brien A,Chapman ME,Mesecar AD,Baker SC
A-11122 was used in Western Blotting to identify amino acid substitutions within the ubiquitin-binding surface of PLP2 that reduced DUB activity.
Mon May 18 00:00:00 EDT 2020
Cdc42 Mediates Cancer Cell Chemotaxis in Perineural Invasion.
Molecular cancer research : MCR
Chernichenko N,Omelchenko T,Deborde S,Bakst RL,He S,Chen CH,Gusain L,Vakiani E,Katabi N,Hall A,Wong RJ
A-11122 was used in Proximity Ligation Assay (PLA) to establish the GDNF-RET-β-Pix-Cdc42 pathway as a directional regulator of pancreatic cancer cell migration toward nerves, highlight the importance of directional migration in PNI, and offer novel targets for therapy.
Mon Jun 01 00:00:00 EDT 2020
A Bird's Eye View on the Origin of Aortic Hemogenic Endothelial Cells.
Frontiers in cell and developmental biology
Seco P,Martins GG,Jacinto A,Tavares AT
A-11122 was used in Immunocytochemistry-immunoflourescence to discuss the current knowledge on the origin of aortic HECs with a particular focus on the evidence provided by studies in the avian embryo, a model that pioneered the field of developmental hematopoiesis.
Fri Dec 18 00:00:00 EST 2020
BAG5 Promotes Alpha-Synuclein Oligomer Formation and Functionally Interacts With the Autophagy Adaptor Protein p62.
Frontiers in cell and developmental biology
Friesen EL,Zhang YT,Earnshaw R,De Snoo ML,O'Hara DM,Agapova V,Chau H,Ngana S,Chen KS,Kalia LV,Kalia SK
A-11122 was used in Western Blotting to identify a novel interaction between BAG5 and p62/sequestosome-1.
Mon Sep 28 00:00:00 EDT 2020
SLC20A1 Is Involved in Urinary Tract and Urorectal Development.
Frontiers in cell and developmental biology
Rieke JM,Zhang R,Braun D,Yilmaz Ö,Japp AS,Lopes FM,Pleschka M,Hilger AC,Schneider S,Newman WG,Beaman GM,Nordenskjöld A,Ebert AK,Promm M,Rösch WH,Stein R,Hirsch K,Schäfer FM,Schmiedeke E,Boemers TM,Lacher M,Kluth D,Gosemann JH,Anderberg M,Barker G,Holmdahl G,Läckgren G,Keene D,Cervellione RM,Giorgio E,Di Grazia M,Feitz WFJ,Marcelis CLM,Van Rooij IALM,Bökenkamp A,Beckers GMA,Keegan CE,Sharma A,Dakal TC,Wittler L,Grote P,Zwink N,Jenetzky E,Brusco A,Thiele H,Ludwig M,Schweizer U,Woolf AS,Odermatt B,Reutter H
A-11122 was used in Immunohistochemistry to study the role of SLC20A1 in urinary tract development using a zebrafish model.
Wed Jun 16 00:00:00 EDT 2021
Drosophila VCP/p97 Mediates Dynein-Dependent Retrograde Mitochondrial Motility in Axons.
Frontiers in cell and developmental biology
Gonzalez AE,Wang X
A-11122 was used in Immunohistochemistry to live image mitochondria and dense core vesicles in Drosophila third instar larval axons and perform genetic interaction experiments to study the role of valosin-containing protein in axon transport.
Mon Sep 28 00:00:00 EDT 2020
Blockade of Glutathione Metabolism in IDH1-Mutated Glioma.
Molecular cancer therapeutics
Tang X,Fu X,Liu Y,Yu D,Cai SJ,Yang C
A-11122 was used in Western Blotting to investigate if isocitrate dehydrogenases 1 mutation results in the disruption of NADP+/NADPH balance and an increased demand for glutathione (GSH) metabolism.
Wed Jan 01 00:00:00 EST 2020
Optogenetic-induced sympathetic neuromodulation of brown adipose tissue thermogenesis.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Lyons CE,Razzoli M,Larson E,Svedberg D,Frontini A,Cinti S,Vulchanova L,Sanders M,Thomas M,Bartolomucci A
A-11122 was used in Immunohistochemistry to demonstrate that local optogenetic stimulation of the sympathetic nerves is sufficient to elicit BAT and core thermogenesis, thus suggesting that peripheral neuromodulation has the potential to be exploited as an alternative to pharmacotherapies to elicit organ activation and thus ameliorate type 2 diabetes and/or obesity.
Sat Feb 01 00:00:00 EST 2020
DIAPH1 regulates ciliogenesis and trafficking in primary cilia.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Palander O,Trimble WS
A-11122 was used in Western Blot to examine the role of DIAPH1 proteins in primary cilia maintenance.
Tue Dec 01 00:00:00 EST 2020
Myogenic Cell Expression of Intercellular Adhesion Molecule-1 Contributes to Muscle Regeneration after Injury.
The American journal of pathology
Martin RA,Buckley KH,Mankowski DC,Riley BM,Sidwell AN,Douglas SL,Worth RG,Pizza FX
A-11122 was used in Immunohistochemistry to investigate intercellular adhesion molecule-1 (ICAM-1), a membrane protein that mediates cell-to-cell adhesion and communication, as a mechanism through which the inflammatory response facilitates muscle regeneration after injury.
Thu Oct 01 00:00:00 EDT 2020
Chemogenetics-mediated acute inhibition of excitatory neuronal activity improves stroke outcome.
Experimental neurology
Wang YC,Galeffi F,Wang W,Li X,Lu L,Sheng H,Hoffmann U,Turner DA,Yang W
A-11122 was used in Immunohistochemistry to demonstrate that acute inhibition of a subset of excitatory neurons after ischemic stroke can prevent brain injury and improve functional outcome.
Wed Apr 01 00:00:00 EDT 2020
Exploiting Pre-Existing CD4+ T Cell Help from Bacille Calmette-Guérin Vaccination to Improve Antiviral Antibody Responses.
Journal of immunology (Baltimore, Md. : 1950)
Ng TW,Wirchnianski AS,Wec AZ,Fels JM,Johndrow CT,Saunders KO,Liao HX,Chan J,Jacobs WR,Chandran K,Porcelli SA
A-11122 was used in Immunohistochemistry (Paraffin) to to investigate the potential for using CD4+ T cells arising in response to Bacille Calmette-Guérin as a source of help for driving antibody responses against viral vaccines.
Wed Jul 15 00:00:00 EDT 2020
Exploiting Pre-Existing CD4+ T Cell Help from Bacille Calmette-Guérin Vaccination to Improve Antiviral Antibody Responses.
Journal of immunology (Baltimore, Md. : 1950)
Ng TW,Wirchnianski AS,Wec AZ,Fels JM,Johndrow CT,Saunders KO,Liao HX,Chan J,Jacobs WR,Chandran K,Porcelli SA
A-11122 was used in Immunohistochemistry (Paraffin) to to investigate the potential for using CD4+ T cells arising in response to Bacille Calmette-Guérin as a source of help for driving antibody responses against viral vaccines.
Wed Jul 15 00:00:00 EDT 2020
Loss of mu and delta opioid receptors on neurons expressing dopamine receptor D1 has no effect on reward sensitivity.
Neuropharmacology
Harda Z,Spyrka J,Jastrzębska K,Szumiec Ł,Bryksa A,Klimczak M,Polaszek M,Gołda S,Zajdel J,Misiołek K,Błasiak A,Rodriguez Parkitna J
A-11122 was used in Immunohistochemistry to show that knocking down mu and delta receptors on dopamine receptor D1-expressing cells does not appreciably affect some of the reward-driven behaviors previously attributed to opioid signaling.
Tue Dec 01 00:00:00 EST 2020
Sex and Death: Identification of Feedback Neuromodulation Balancing Reproduction and Survival.
Neuroscience bulletin
Gao C,Guo C,Peng Q,Cao J,Shohat-Ophir G,Liu D,Pan Y
A-11122 was used in Immunohistochemistry to understand the molecular underpinnings of how different organisms choose reproductive strategies and balance reproduction and survival.
Tue Dec 01 00:00:00 EST 2020
The Na+ pump Ena1 is a yeast epsin-specific cargo requiring its ubiquitylation and phosphorylation sites for internalization.
Journal of cell science
Sen A,Hsieh WC,Hanna CB,Hsu CC,Pearson M,Tao WA,Aguilar RC
A-11122 was used in Western Blotting to indicate that the yeast Na+ pump Ena1 is an epsin (Ent1 and Ent2 in yeast)-specific cargo and that its internalization requires K1090, which likely undergoes Art3-dependent ubiquitylation.
Fri Aug 21 00:00:00 EDT 2020
Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.
Journal of cell science
D'Ambrosio JM,Albanèse V,Lipp NF,Fleuriot L,Debayle D,Drin G,Čopič A
A-11122 was used in Western Blotting to show that Osh6 localization to contact sites depends on its interaction with the cytosolic tail of the ER-PM tether Ist2, a homolog of TMEM16 proteins.
Thu Jun 04 00:00:00 EDT 2020
OPTN recruitment to a Golgi-proximal compartment regulates immune signalling and cytokine secretion.
Journal of cell science
O'Loughlin T,Kruppa AJ,Ribeiro ALR,Edgar JR,Ghannam A,Smith AM,Buss F
A-11122 was used in Western Blotting to uncover a crucial role for OPTN in dampening NF-κB and IRF3 signalling through the sequestration of LUBAC and other positive regulators in this viral RNA-induced compartment, leading to altered pro-inflammatory cytokine secretion.
Mon Jun 15 00:00:00 EDT 2020
Canonical nucleators are dispensable for stress granule assembly in Drosophila intestinal progenitors.
Journal of cell science
Buddika K,Ariyapala IS,Hazuga MA,Riffert D,Sokol NS
A-11122 was used in Immunocytochemistry to report that the Drosophila orthologs of the mammalian SG components AGO1, ATX2, CAPRIN, eIF4E, FMRP, G3BP, LIN-28, PABP and TIAR are enriched in adult fly intestinal progenitor cells, where they accumulate in small cytoplasmic messenger ribonucleoprotein complexes (mRNPs).
Mon May 18 00:00:00 EDT 2020
STIM1 interacts with termini of Orai channels in a sequential manner.
Journal of cell science
Niu L,Wu F,Li K,Li J,Zhang SL,Hu J,Wang Q
A-11122 was used in Western Blotting to analyze STIM1-Orai interactions using synthetic peptides derived from the N- and C-termini of Orai channels (Orai-NT and Orai-CT, respectively) and purified fragments of STIM1.
Wed Apr 29 00:00:00 EDT 2020
Rabgap1 promotes recycling of active β1 integrins to support effective cell migration.
Journal of cell science
Samarelli AV,Ziegler T,Meves A,Fässler R,Böttcher RT
A-11122 was used in Western Blot, Immunocytochemistry to identify Rabgap1 as an important factor for conformation-specific integrin trafficking and define the role of Rabgap1 in β1-integrin-mediated cell migration in mouse fibroblasts and breast cancer cells.
Wed Sep 23 00:00:00 EDT 2020
Rabgap1 promotes recycling of active β1 integrins to support effective cell migration.
Journal of cell science
Samarelli AV,Ziegler T,Meves A,Fässler R,Böttcher RT
A-11122 was used in Western Blot, Immunocytochemistry to identify Rabgap1 as an important factor for conformation-specific integrin trafficking and define the role of Rabgap1 in β1-integrin-mediated cell migration in mouse fibroblasts and breast cancer cells.
Wed Sep 23 00:00:00 EDT 2020
Role of a versatile peptide motif controlling Hox nuclear export and autophagy in the Drosophila fat body.
Journal of cell science
Duffraisse M,Paul R,Carnesecchi J,Hudry B,Banreti A,Reboulet J,Ajuria L,Lohmann I,Merabet S
A-11122 was used in Immunohistochemistry to reveal the extreme molecular versatility of a unique short peptide motif for controlling the context-dependent activity of Hox proteins both at transcriptional and non-transcriptional levels.
Wed Sep 23 00:00:00 EDT 2020
Schizosaccharomyces pombe DNA translocases Rrp1 and Rrp2 have distinct roles at centromeres and telomeres that ensure genome stability.
Journal of cell science
Barg-Wojas A,Muraszko J,Kramarz K,Schirmeisen K,Baranowska G,Carr AM,Dziadkowiec D
A-11122 was used in ChIP assay to identify roles for Rrp1 and Rrp2 in maintaining centromere function by modulating histone dynamics, contributing to the preservation of genome stability during vegetative cell growth.
Mon Feb 10 00:00:00 EST 2020
Node-Localized Transporters of Phosphorus Essential for Seed Development in Rice.
Plant & cell physiology
Che J,Yamaji N,Miyaji T,Mitani-Ueno N,Kato Y,Shen RF,Ma JF
A-11122 was used in Immunocytochemistry to show that OsPHO1;2 expressed in node I is responsible for the unloading of P from the xylem of EVBs, while OsPHO1;1 is involved in reloading P into the phloem of DVBs for subsequent allocation of P to seeds.
Sat Aug 01 00:00:00 EDT 2020
Development of the intrinsic innervation of the small bowel mucosa and villi.
American journal of physiology. Gastrointestinal and liver physiology
Hao MM,Fung C,Boesmans W,Lowette K,Tack J,Vanden Berghe P
A-11122 was used in Immunohistochemistry to show that enteric neurites project into the lamina propria as early as E13.5 in the mouse, before development of the submucous plexus and before formation of intestinal villi.
Wed Jan 01 00:00:00 EST 2020
Olig2-Induced Semaphorin Expression Drives Corticospinal Axon Retraction After Spinal Cord Injury.
Cerebral cortex (New York, N.Y. : 1991)
Ueno M,Nakamura Y,Nakagawa H,Niehaus JK,Maezawa M,Gu Z,Kumanogoh A,Takebayashi H,Lu QR,Takada M,Yoshida Y
A-11122 was used in Immunohistochemistry to demonstrate that semaphorins function as axon repellents following SCI, and reveal a novel transcriptional mechanism for controlling semaphorin levels around injured neurons to create zones hostile to axon regrowth.
Thu Oct 01 00:00:00 EDT 2020
NCAM2 Regulates Dendritic and Axonal Differentiation through the Cytoskeletal Proteins MAP2 and 14-3-3.
Cerebral cortex (New York, N.Y. : 1991)
Parcerisas A,Pujadas L,Ortega-Gascó A,Perelló-Amorós B,Viais R,Hino K,Figueiro-Silva J,La Torre A,Trullás R,Simó S,Lüders J,Soriano E
A-11122 was used in Western Blot to demonstrate a role for NCAM2 in dendritic formation and maintenance, and in neural polarization and migration, through interaction of NCAM2 with microtubule-associated proteins.
Mon May 18 00:00:00 EDT 2020
NCAM2 Regulates Dendritic and Axonal Differentiation through the Cytoskeletal Proteins MAP2 and 14-3-3.
Cerebral cortex (New York, N.Y. : 1991)
Parcerisas A,Pujadas L,Ortega-Gascó A,Perelló-Amorós B,Viais R,Hino K,Figueiro-Silva J,La Torre A,Trullás R,Simó S,Lüders J,Soriano E
A-11122 was used in Western Blot to demonstrate a role for NCAM2 in dendritic formation and maintenance, and in neural polarization and migration, through interaction of NCAM2 with microtubule-associated proteins.
Mon May 18 00:00:00 EDT 2020
Identification of Split-GAL4 Drivers and Enhancers That Allow Regional Cell Type Manipulations of the Drosophila melanogaster Intestine.
Genetics
Ariyapala IS,Holsopple JM,Popodi EM,Hartwick DG,Kahsai L,Cook KR,Sokol NS
A-11122 was used in Western Blot to show that intestinal enhancers identified with the split-GAL4 system can confer equivalent expression patterns on other transgenic reporters.
Tue Dec 01 00:00:00 EST 2020
Defining the Boundaries of Polycomb Domains in Drosophila.
Genetics
De S,Gehred ND,Fujioka M,Chan FW,Jaynes JB,Kassis JA
A-11122 was used in Immunohistochemistry to suggest that active transcription of a boundary gene may restrict the range of enhancer activity of a Polycomb-regulated gene.
Sun Nov 01 00:00:00 EDT 2020
Neuronal tangential migration from Nkx2.1-positive hypothalamus.
Brain structure & function
Murcia-Ramón R,Company V,Juárez-Leal I,Andreu-Cervera A,Almagro-García F,Martínez S,Echevarría D,Puelles E
A-11122 was used in Immunohistochemistry to describe the contribution of interneurons from the Nkx2.1-positive hypothalamic territory into two different rostrocaudal territories: the mes-diencephalic reticular formation through a caudal tangential migration and the prethalamic zona incerta complex through a dorsocaudal tangential migration.
Tue Dec 01 00:00:00 EST 2020
Mutational Analysis of the Structure and Function of the Chaperoning Domain of UNC-45B.
Biophysical journal
Gaziova I,Moncrief T,Christian CJ,Villarreal M,Powell S,Lee H,Qadota H,White MA,Benian GM,Oberhauser AF
A-11122 was used in Immunohistochemistry to show that, to our surprise, the R805W mutation appeared to have the most drastic detrimental effect on the structure and organization of the worm sarcomeres, indicating a crucial role of R805 in UCS-client interactions.
Tue Aug 18 00:00:00 EDT 2020
Comparative analyses of transgene expression patterns after intra-striatal injections of rAAV2-retro in rats and rhesus monkeys: A light and electron microscopic study.
The European journal of neuroscience
Albaugh DL,Smith Y,Galvan A
A-11122 was used in Immunohistochemistry to evaluate the suitability of retrogradely-transducing viral vector rAAV2-retro in the nonhuman primate basal ganglia.
Tue Dec 01 00:00:00 EST 2020
LIM and SH3 protein 1 localizes to the leading edge of protruding lamellipodia and regulates axon development.
Molecular biology of the cell
Pollitt SL,Myers KR,Yoo J,Zheng JQ
A-11122 was used in Immunocytochemistry-immunoflourescence to determine the role of LIM and SH3 protein 1 (LASP1) in axon development through localisation along the leading edge of lamellipodia.
Sun Nov 15 00:00:00 EST 2020
Mutations in the J domain of DNAJB6 cause dominant distal myopathy.
Neuromuscular disorders : NMD
Palmio J,Jonson PH,Inoue M,Sarparanta J,Bengoechea R,Savarese M,Vihola A,Jokela M,Nakagawa M,Noguchi S,Olivé M,Masingue M,Kerty E,Hackman P,Weihl CC,Nishino I,Udd B
A-11122 was used in Immuno-assay to identify two novel mutations in DNAJB6 J domain, c.149C>T (p.A50V) and c.161A>C (p.E54A) as the cause of distal myopathy.
Wed Jan 01 00:00:00 EST 2020
Rewarding compounds identified from the medicinal plant Rhodiola rosea.
The Journal of experimental biology
Michels B,Franke K,Weiglein A,Sultani H,Gerber B,Wessjohann LA
A-11122 was used in Immunohistochemistry to discuss whether similar rewarding effects, should they be observed in humans, would indicate a habit-forming or addictive potential.
Wed Aug 26 00:00:00 EDT 2020
Structural plasticity of GABAergic and glutamatergic networks in the motor thalamus of parkinsonian monkeys.
The Journal of comparative neurology
Swain AJ,Galvan A,Wichmann T,Smith Y
A-11122 was used in Immunohistochemistry to suggest that the corticothalamic system undergoes significant synaptic remodeling in the parkinsonian state.
Mon Jun 01 00:00:00 EDT 2020
Characterizing the morphology of somatostatin-expressing interneurons and their synaptic innervation pattern in the barrel cortex of the GFP-expressing inhibitory neurons mouse.
The Journal of comparative neurology
Zhou X,Mansori I,Fischer T,Witte M,Staiger JF
A-11122 was used in Immunohistochemistry to reveal unique features in terms of the morphology and output of GIN cells, which can help to better understand their diversity and structure-function relationships.
Sat Feb 01 00:00:00 EST 2020
Serotonergic modulation across sensory modalities.
Journal of neurophysiology
Sizemore TR,Hurley LM,Dacks AM
A-11122 was used in Immunohistochemistry to study different consequences of neuromodulation using serotonergic signaling pathways.
Mon Jun 01 00:00:00 EDT 2020
Molecular characterization and distribution of the voltage-gated sodium channel, Para, in the brain of the grasshopper and vinegar fly.
Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology
Wang H,Foquet B,Dewell RB,Song H,Dierick HA,Gabbiani F
A-11122 was used in Immunohistochemistry to identify an ortholog in the transcriptome of Schistocerca.
Sun Mar 01 00:00:00 EST 2020
An RNAi screen for secreted factors and cell-surface players in coordinating neuron and glia development in Drosophila.
Molecular brain
Liu Z,Chen Y,Rao Y
A-11122 was used in Immunohistochemistry-immunofluorescence to show neuroglian coordinates R-cell and wrapping glia development in the wrapping glia and eye disc.
Fri Jan 03 00:00:00 EST 2020
GDE2-Dependent Activation of Canonical Wnt Signaling in Neurons Regulates Oligodendrocyte Maturation.
Cell reports
Choi BR,Cave C,Na CH,Sockanathan S
A-11122 was used in Immunohistochemistry on frozen tissues to show that Glycerophosphodiester phosphodiesterase 2 (GDE2) signaling underlies a neuronal pathway that promotes oligodendrocyte maturation through the release of soluble neuronally derived factors.
Tue May 05 00:00:00 EDT 2020
Circuits that encode and guide alcohol-associated preference.
eLife
Scaplen KM,Talay M,Nunez KM,Salamon S,Waterman AG,Gang S,Song SL,Barnea G,Kaun KR
A-11122 was used in Immunohistochemistry to demonstrate that circuits required for alcohol-associated preference shift from population level dopaminergic activation to select dopamine neurons that predict behavioral choice in Drosophila melanogaster.
Thu Jun 04 00:00:00 EDT 2020
Expansion microscopy of C. elegans.
eLife
Yu CJ,Barry NC,Wassie AT,Sinha A,Bhattacharya A,Asano S,Zhang C,Chen F,Hobert O,Goodman MB,Haspel G,Boyden ES
A-11122 was used in Immunohistochemistry-immunofluorescence to develop methods of carrying out expansion microscopy on C. elegans (ExCel), in order to map proteins, identify new proteins and analyse gene expression.
Fri May 01 00:00:00 EDT 2020
TRPV4 disrupts mitochondrial transport and causes axonal degeneration via a CaMKII-dependent elevation of intracellular Ca2.
Nature communications
Woolums BM,McCray BA,Sung H,Tabuchi M,Sullivan JM,Ruppell KT,Yang Y,Mamah C,Aisenberg WH,Saavedra-Rivera PC,Larin BS,Lau AR,Robinson DN,Xiang Y,Wu MN,Sumner CJ,Lloyd TE
A-11122 was used in Immunohistochemistry-immunofluorescence to highlight an integral role for CaMKII in neuronal TRPV4-associated Ca2+ responses, the importance of tightly regulated Ca2+ dynamics for mitochondrial axonal transport, and the therapeutic promise of TRPV4 antagonists for patients with TRPV4-related neurodegenerative diseases.
Fri May 29 00:00:00 EDT 2020
2-Phenylethylamine (PEA) Ameliorates Corticosterone-Induced Depression-Like Phenotype via the BDNF/TrkB/CREB Signaling Pathway.
International journal of molecular sciences
Lee YJ,Kim HR,Lee CY,Hyun SA,Ko MY,Lee BS,Hwang DY,Ka M
A-11122 was used in Immunocytochemistry-immunoflourescence to investigate the molecular mechanism of the antidepressant effect of 2-Phenethylamine(PEA) in a mouse model of corticosterone-induced depression.
Mon Nov 30 00:00:00 EST 2020
Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29.
Nature communications
Crawley-Snowdon H,Yang JC,Zaccai NR,Davis LJ,Wartosch L,Herman EK,Bright NA,Swarbrick JS,Collins BM,Jackson LP,Seaman MNJ,Luzio JP,Dacks JB,Neuhaus D,Owen DJ
A-11122 was used in Immunocytochemistry-immunoflourescence to study VARP and TBC1D5 binding the same site on VPS29 and investigate their competing for binding VPS29 in vivo.
Tue Oct 06 00:00:00 EDT 2020
Striatal direct and indirect pathway neurons differentially control the encoding and updating of goal-directed learning.
eLife
Peak J,Chieng B,Hart G,Balleine BW
A-11122 was used in Immunohistochemistry to support response flexibility.
Fri Nov 20 00:00:00 EST 2020
Interneuron-specific plasticity at parvalbumin and somatostatin inhibitory synapses onto CA1 pyramidal neurons shapes hippocampal output.
Nature communications
Udakis M,Pedrosa V,Chamberlain SEL,Clopath C,Mellor JR
A-11122 was used in Immunohistochemistry to show that circuit-level modelling reveals that PV-iLTD and SST-iLTP cooperate to stabilise place cells while facilitating representation of multiple unique environments within the hippocampal network.
Wed Sep 02 00:00:00 EDT 2020
Repression of interrupted and intact rDNA by the SUMO pathway in Drosophila melanogaster.
eLife
Luo Y,Fefelova E,Ninova M,Chen YA,Aravin AA
A-11122 was used in Chromatin immunoprecipitation to suggest that the SUMO pathway is responsible for both repression of interrupted units and control of intact rDNA expression.
Mon Nov 09 00:00:00 EST 2020
Cytotoxicity and Mitochondrial Dysregulation Caused by α-Synuclein in Dictyostelium discoideum.
Cells
Fernando S,Allan CY,Mroczek K,Pearce X,Sanislav O,Fisher PR,Annesley SJ
A-11122 was used in Immunocytochemistry-immunoflourescence to show that the C-terminus of α-synuclein is required and sufficient for the localisation of the protein to the cell cortex in D. discoideum.
Wed Oct 14 00:00:00 EDT 2020
Inappropriate cathepsin K secretion promotes its enzymatic activation driving heart and valve malformation.
JCI insight
Lu PN,Moreland T,Christian CJ,Lund TC,Steet RA,Flanagan-Steet H
A-11122 was used in Immunohistochemistry to investigate the contribution of cathepsins during cardiovascular development and congenital disease.
Thu Oct 15 00:00:00 EDT 2020
Cytoplasmic sharing through apical membrane remodeling.
eLife
Peterson NG,Stormo BM,Schoenfelder KP,King JS,Lee RR,Fox DT
A-11122 was used in Immunocytochemistry to reveal a new role for apical membrane remodeling in converting a multicellular epithelium into a giant multinucleate cytoplasm.
Wed Oct 14 00:00:00 EDT 2020
RNase L promotes the formation of unique ribonucleoprotein granules distinct from stress granules.
The Journal of biological chemistry
Burke JM,Lester ET,Tauber D,Parker R
A-11122 was used in Immunoprecipitation to demonstrate that RLBs represent a unique RNP granule with a protein and RNA composition distinct from that of SGs in response to dsRNA lipofection in human cells, using immunofluorescence, live-cell imaging, and MS-based analyses.
Fri Feb 07 00:00:00 EST 2020
Optimizing clozapine for chemogenetic neuromodulation of somatosensory cortex.
Scientific reports
Cho J,Ryu S,Lee S,Kim J,Kim HI
A-11122 was used in Immunohistochemistry to show that low doses of CLZ (0.1 and 0.01 mg/kg) successfully induced neural responses without off-target effects.
Tue Apr 07 00:00:00 EDT 2020
Persistent activation of central amygdala CRF neurons helps drive the immediate fear extinction deficit.
Nature communications
Jo YS,Namboodiri VMK,Stuber GD,Zweifel LS
A-11122 was used in Immunohistochemistry to demonstrate CeA-CRF neurons are an important substrate for the persistence of fear and have broad implications for the neural basis of persistent negative affective behavioral states.
Wed Jan 22 00:00:00 EST 2020
A genetic screen in Drosophila reveals an unexpected role for the KIP1 ubiquitination-promoting complex in male fertility.
PLoS genetics
Li W,Liang J,Outeda P,Turner S,Wakimoto BT,Watnick T
A-11122 was used in Immunoprecipitation to identify a new complex that is involved in male fertility in Drosophila melanogaster.
Tue Dec 01 00:00:00 EST 2020
Tetraspanin 33 (TSPAN33) regulates endocytosis and migration of human B lymphocytes by affecting the tension of the plasma membrane.
The FEBS journal
Navarro-Hernandez IC,López-Ortega O,Acevedo-Ochoa E,Cervantes-Díaz R,Romero-Ramírez S,Sosa-Hernández VA,Meza-Sánchez DE,Juárez-Vega G,Pérez-Martínez CA,Chávez-Munguía B,Galván-Hernández A,Antillón A,Ortega-Blake I,Santos-Argumedo L,Hernández-Hernández JM,Maravillas-Montero JL
A-11122 was used in Western Blotting to illustrate that TSPAN33 regulates adhesion and migration of B lymphocytes by cotrolling the mechanical properties of their plasma membrane.
Sat Aug 01 00:00:00 EDT 2020
Fatty acid β-oxidation is required for the differentiation of larval hematopoietic progenitors in Drosophila.
eLife
Tiwari SK,Toshniwal AG,Mandal S,Mandal L
A-11122 was used in Immunohistochemistry to demonstrate that Fatty Acid Oxidation is essential for the differentiation of blood cell progenitors.
Fri Jun 12 00:00:00 EDT 2020
GAD2 Expression Defines a Class of Excitatory Lateral Habenula Neurons in Mice that Project to the Raphe and Pontine Tegmentum.
eNeuro
Quina LA,Walker A,Morton G,Han V,Turner EE
A-11122 was used in Immunohistochemistry to conclude that GAD2 expression can serve as a marker for functional studies of excitatory neurons serving specific LHb output pathways in mice.
Fri Jun 18 00:00:00 EDT 2021
Identification of genomic enhancers through spatial integration of single-cell transcriptomics and epigenomics.
Molecular systems biology
Bravo González-Blas C,Quan XJ,Duran-Romaña R,Taskiran II,Koldere D,Davie K,Christiaens V,Makhzami S,Hulselmans G,de Waegeneer M,Mauduit D,Poovathingal S,Aibar S,Aerts S
A-11122 was used in Immunohistochemistry-immunofluorescence to provide a comprehensive spatial characterization of gene regulation in a 2D tissue.
Fri May 01 00:00:00 EDT 2020
UNBRANCHED3 Expression and Inflorescence Development is Mediated by UNBRANCHED2 and the Distal Enhancer, KRN4, in Maize.
PLoS genetics
Du Y,Liu L,Peng Y,Li M,Li Y,Liu D,Li X,Zhang Z
A-11122 was used in Chromatin immunoprecipitation to genetically characterize the maize enhancer, kernel row number4.
Wed Apr 01 00:00:00 EDT 2020
TDP-43 prevents retrotransposon activation in the Drosophila motor system through regulation of Dicer-2 activity.
BMC biology
Romano G,Klima R,Feiguin F
A-11122 was used in Western Blotting to describe a novel physiological role of endogenous TDP-43 in the prevention of RTE-induced neurological alterations through the modulation of Dicer-2 activity and the siRNA pathway.
Fri Jul 03 00:00:00 EDT 2020
An activity-dependent local transport regulation via degradation and synthesis of KIF17 underlying cognitive flexibility.
Science advances
Iwata S,Morikawa M,Takei Y,Hirokawa N
A-11122 was used in Western Blot, Immunocytochemistry to show that the molecular motor KIF17 is locally degraded and synthesized in an N-methyl-d-aspartate receptor (NMDAR)-mediated activity-dependent manner.
Tue Dec 01 00:00:00 EST 2020
An activity-dependent local transport regulation via degradation and synthesis of KIF17 underlying cognitive flexibility.
Science advances
Iwata S,Morikawa M,Takei Y,Hirokawa N
A-11122 was used in Western Blot, Immunocytochemistry to show that the molecular motor KIF17 is locally degraded and synthesized in an N-methyl-d-aspartate receptor (NMDAR)-mediated activity-dependent manner.
Tue Dec 01 00:00:00 EST 2020
KRAB-zinc finger protein gene expansion in response to active retrotransposons in the murine lineage.
eLife
Wolf G,de Iaco A,Sun MA,Bruno M,Tinkham M,Hoang D,Mitra A,Ralls S,Trono D,Macfarlan TS
A-11122 was used in Chromatin immunoprecipitation to demonstrate that many recently emerged murine KRAB-ZFPs also bind to TEs, including the active ETn, IAP, and L1 families.
Mon Jun 01 00:00:00 EDT 2020
Mutator Foci Are Regulated by Developmental Stage, RNA, and the Germline Cell Cycle in Caenorhabditis elegans.
G3 (Bethesda, Md.)
Uebel CJ,Agbede D,Wallis DC,Phillips CM
A-11122 was used in Immunocytochemistry to reveal that punctate Mutator foci arise in the progenitor germ cells of early embryos and persist throughout all larval stages.
Mon Oct 05 00:00:00 EDT 2020
Role of serotonergic dorsal raphe neurons in hypercapnia-induced arousals.
Nature communications
Kaur S,De Luca R,Khanday MA,Bandaru SS,Thomas RC,Broadhurst RY,Venner A,Todd WD,Fuller PM,Arrigoni E,Saper CB
A-11122 was used in Immunohistochemistry-immunofluorescence to indicate that the serotonergic input from the dorsal raphe to the parabrachial nucleus is critical for modulating the sensitivity of the PBelCGRP neurons that cause arousal to rising levels of blood CO2 during hypercapnia.
Tue Jun 02 00:00:00 EDT 2020
STAT2 Limits Host Species Specificity of Human Metapneumovirus.
Viruses
Rogers MC,Miranda-Katz M,Zhang Y,Oury TD,Uccellini MB,García-Sastre A,Williams JV
A-11122 was used in Western Blotting to show that HMPV inhibits expression of both STAT1 and STAT2 in human and primate cells, but not in mouse cells.
Sat Jul 04 00:00:00 EDT 2020
Analysis of Virus and Host Proteomes During Productive HSV-1 and VZV Infection in Human Epithelial Cells.
Frontiers in microbiology
Ouwendijk WJD,Dekker LJM,van den Ham HJ,Lenac Rovis T,Haefner ES,Jonjic S,Haas J,Luider TM,Verjans GMGM
A-11122 was used in Immunocytochemistry to determine the temporal changes in virus and host protein expression during productive HSV-1 and VZV infection in the same cell type.
Wed Mar 22 00:00:00 EDT 2023
Phosphorylation of NMDA receptors by cyclin B/CDK1 modulates calcium dynamics and mitosis.
Communications biology
Rosendo-Pineda MJ,Vicente JJ,Vivas O,Pacheco J,Loza-Huerta A,Sampieri A,Wordeman L,Moreno C,Vaca L
A-11122 was used in Immunoprecipitation to investigate the role of NMDA receptors during mitosis in individual cells.
Thu Nov 12 00:00:00 EST 2020
Action representation in the mouse parieto-frontal network.
Scientific reports
Tombaz T,Dunn BA,Hovde K,Cubero RJ,Mimica B,Mamidanna P,Roudi Y,Whitlock JR
A-11122 was used in Immunohistochemistry to investigate whether mirror-like neurons occur in similar networks in other social species, such as rodents, or if they can be measured in such models using paradigms where observers passively view a demonstrator.
Fri Mar 27 00:00:00 EDT 2020
Comparing the performance of mScarlet-I, mRuby3, and mCherry as FRET acceptors for mNeonGreen.
PloS one
McCullock TW,MacLean DM,Kammermeier PJ
A-11122 was used in Western Blot to assess the ability of these two proteins, along with mCherry, to act as a FRET acceptor for the bright, monomeric, green-yellow FP mNeonGreen using intensiometric FRET and 2-photon Fluorescent Lifetime Imaging Microscopy (FLIM) FRET techniques.
Mon Apr 27 00:00:00 EDT 2020
Selective Inactivation of Reelin in Inhibitory Interneurons Leads to Subtle Changes in the Dentate Gyrus But Leaves Cortical Layering and Behavior Unaffected.
Cerebral cortex (New York, N.Y. : 1991)
Pahle J,Muhia M,Wagener RJ,Tippmann A,Bock HH,Graw J,Herz J,Staiger JF,Drakew A,Kneussel M,Rune GM,Frotscher M,Brunne B
A-11122 was used in Immunohistochemistry-immunofluorescence to show that IN-specific Reelin knockout mice exhibit normal neuronal layering and normal behavior, including spatial reference memory.
Sat Mar 14 00:00:00 EDT 2020
The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway.
eLife
Stührwohldt N,Scholl S,Lang L,Katzenberger J,Schumacher K,Schaller A
A-11122 was used in Western Blotting to investigate the influence of the secretory pathway on peptide biogenesis.
Thu Apr 16 00:00:00 EDT 2020
Unc-4 acts to promote neuronal identity and development of the take-off circuit in the Drosophila CNS.
eLife
Lacin H,Williamson WR,Card GM,Skeath JB,Truman JW
A-11122 was used in Immunohistochemistry-immunofluorescence to nvestigated the developmental and molecular basis of behavior by focusing on lineage-specific functions of the homeodomain transcription factor, Unc-4 in Drosophila.
Fri Mar 27 00:00:00 EDT 2020
Myc plays an important role in Drosophila P-M hybrid dysgenesis to eliminate germline cells with genetic damage.
Communications biology
Ota R,Kobayashi S
A-11122 was used in Immunohistochemistry-immunofluorescence to demonstrate that Myc plays an important role in eliminating germline cells in the context of hybrid dysgenesis (HD).
Wed Apr 22 00:00:00 EDT 2020
Identification of ALPPL2 as a Naive Pluripotent State-Specific Surface Protein Essential for Human Naive Pluripotency Regulation.
Cell reports
Bi Y,Tu Z,Zhang Y,Yang P,Guo M,Zhu X,Zhao C,Zhou J,Wang H,Wang Y,Gao S
A-11122 was used in Flow cytometry/Cell sorting to identify a functional surface marker for human naive pluripotency.
Tue Mar 17 00:00:00 EDT 2020
Epistatic Interplay between Type IV Secretion Effectors Engages the Small GTPase Rab2 in the Brucella Intracellular Cycle.
mBio
Smith EP,Cotto-Rosario A,Borghesan E,Held K,Miller CN,Celli J
A-11122 was used in Western Blotting to demonstrate that T4SS effectors BspB and RicA display epistatic interactions in Brucella replication.
Tue Mar 31 00:00:00 EDT 2020
A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells.
Nature communications
Sung BH,von Lersner A,Guerrero J,Krystofiak ES,Inman D,Pelletier R,Zijlstra A,Ponik SM,Weaver AM
A-11122 was used in Western Blotting to develop an improved live-cell reporter for exosome monitoring which identified pathfinding behaviour of migrating cells in both 2D and 3D.
Wed Apr 29 00:00:00 EDT 2020
Developmental Potential and Plasticity of Olfactory Epithelium Stem Cells Revealed by Heterotopic Grafting in the Adult Brain.
Stem cell reports
Li Q,Siri T,Bressan C,de Koninck Y,Saghatelyan A
A-11122 was used in Immunohistochemistry to reveal the developmental potential and plasticity of olfactory epithelium (OE)-derived neural stem cells (NSCs) in vivo and show that they can respond to heterotopic neurogenic cues to adapt their phenotype and become functional neurons in ectopic brain regions.
Tue Apr 14 00:00:00 EDT 2020
Age-Related Changes in Synaptic Plasticity Associated with Mossy Fiber Terminal Integration during Adult Neurogenesis.
eNeuro
Murray KD,Liu XB,King AN,Luu JD,Cheng HJ
A-11122 was used in Immunohistochemistry to describe the maturation of mossy fiber bouton (MFB) connectivity with CA3 pyramidal cells in young adult and aged mouse brain.
Thu Aug 31 00:00:00 EDT 2023
Fine tuning of the unfolded protein response by ISRIB improves neuronal survival in a model of amyotrophic lateral sclerosis.
Cell death & disease
Bugallo R,Marlin E,Baltanás A,Toledo E,Ferrero R,Vinueza-Gavilanes R,Larrea L,Arrasate M,Aragón T
A-11122 was used in Immunocytochemistry-immunoflourescence to identify ISRIB-mediated translational reprogramming as a new potential ALS therapy.
Tue May 26 00:00:00 EDT 2020
Fine tuning of the unfolded protein response by ISRIB improves neuronal survival in a model of amyotrophic lateral sclerosis.
Cell death & disease
Bugallo R,Marlin E,Baltanás A,Toledo E,Ferrero R,Vinueza-Gavilanes R,Larrea L,Arrasate M,Aragón T
A-11122 was used in Immunocytochemistry-immunoflourescence to identify ISRIB-mediated translational reprogramming as a new potential ALS therapy.
Tue May 26 00:00:00 EDT 2020
Acute conversion of patient-derived Duchenne muscular dystrophy iPSC into myotubes reveals constitutive and inducible over-activation of TGFβ-dependent pro-fibrotic signaling.
Skeletal muscle
Caputo L,Granados A,Lenzi J,Rosa A,Ait-Si-Ali S,Puri PL,Albini S
A-11122 was used in Immunocytochemistry-immunoflourescence to demonstrate that acute conversion of Duchenne muscular dystrophy (DMD) patient-derived iPSC into skeletal muscles, by the ectopic expression of MyoD and BAF60C, provides a rapid and reliable protocol for an “in dish” DMD model.
Sat May 02 00:00:00 EDT 2020
Divergent receptor proteins confer responses to different karrikins in two ephemeral weeds.
Nature communications
Sun YK,Yao J,Scaffidi A,Melville KT,Davies SF,Bond CS,Smith SM,Flematti GR,Waters MT
A-11122 was used in Western Blotting to suggest that duplication and diversification of KAI2 proteins could confer differential responses to chemical cues produced by environmental disturbance, including fire.
Mon Mar 09 00:00:00 EDT 2020
Inhibition of autophagy curtails visual loss in a model of autosomal dominant optic atrophy.
Nature communications
Zaninello M,Palikaras K,Naon D,Iwata K,Herkenne S,Quintana-Cabrera R,Semenzato M,Grespi F,Ross-Cisneros FN,Carelli V,Sadun AA,Tavernarakis N,Scorrano L
A-11122 was used in Immunocytochemistry, Flow Cytometry to identify AMPK and autophagy as targetable components of autosomal dominant optic atrophy (ADOA) pathogenesis.
Wed Aug 12 00:00:00 EDT 2020
Inhibition of autophagy curtails visual loss in a model of autosomal dominant optic atrophy.
Nature communications
Zaninello M,Palikaras K,Naon D,Iwata K,Herkenne S,Quintana-Cabrera R,Semenzato M,Grespi F,Ross-Cisneros FN,Carelli V,Sadun AA,Tavernarakis N,Scorrano L
A-11122 was used in Immunocytochemistry, Flow Cytometry to identify AMPK and autophagy as targetable components of autosomal dominant optic atrophy (ADOA) pathogenesis.
Wed Aug 12 00:00:00 EDT 2020
Magnesium efflux from Drosophila Kenyon cells is critical for normal and diet-enhanced long-term memory.
eLife
Wu Y,Funato Y,Meschi E,Jovanoski KD,Miki H,Waddell S
A-11122 was used in Immunohistochemistry-immunofluorescence to show that Mg2+ feeding also enhances long-term memory in Drosophila. Normal and Mg2+ enhanced fly memory appears independent of NMDA receptors in the mushroom body and instead requires expression of a conserved CNNM-type Mg2+-efflux transporter encoded by the unextended (uex) gene.
Thu Nov 26 00:00:00 EST 2020
A neural circuit encoding mating states tunes defensive behavior in Drosophila.
Nature communications
Liu C,Zhang B,Zhang L,Yang T,Zhang Z,Gao Z,Zhang W
A-11122 was used in Immunocytochemistry to report that fruit flies exhibit a strong defensive response to mechanical stimuli to their wings.
Fri Aug 07 00:00:00 EDT 2020
Mutations of CNTNAP1 led to defects in neuronal development.
JCI insight
Li W,Yang L,Tang C,Liu K,Lu Y,Wang H,Yan K,Qiu Z,Zhou W
A-11122 was used in Immunohistochemistry to elucidate a critical function of CNTNAP1 in cortical development, providing insights underlying molecular and circuit mechanisms of CNTNAP1-related disease.
Thu Nov 05 00:00:00 EST 2020
Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest.
eLife
Marelli S,Williamson JC,Protasio AV,Naamati A,Greenwood EJ,Deane JE,Lehner PJ,Matheson NJ
A-11122 was used in Western Blotting to show that degradation of PPP2R5 family subunits is both necessary and sufficient for Vif-dependent G2/M cell cycle arrest.
Wed Apr 15 00:00:00 EDT 2020
Exploiting codon usage identifies intensity-specific modifiers of Ras/MAPK signaling in vivo.
PLoS genetics
Sawyer JK,Kabiri Z,Montague RA,Allen SR,Stewart R,Paramore SV,Cohen E,Zaribafzadeh H,Counter CM,Fox DT
A-11122 was used in Western Blotting to screen the Drosophila genome for modifiers specific to either weak or strong Ras-driven eye phenotypes.
Tue Dec 01 00:00:00 EST 2020
Coupled Control of Distal Axon Integrity and Somal Responses to Axonal Damage by the Palmitoyl Acyltransferase ZDHHC17.
Cell reports
Niu J,Sanders SS,Jeong HK,Holland SM,Sun Y,Collura KM,Hernandez LM,Huang H,Hayden MR,Smith GM,Hu Y,Jin Y,Thomas GM
A-11122 was used in Immunohistochemistry to demonstrate that two palmitoylation-dependent pathways mediate the control of somal and distal axon integrity.
Tue Nov 17 00:00:00 EST 2020
Exploration of sensory and spinal neurons expressing gastrin-releasing peptide in itch and pain related behaviors.
Nature communications
Barry DM,Liu XT,Liu B,Liu XY,Gao F,Zeng X,Liu J,Yang Q,Wilhelm S,Yin J,Tao A,Chen ZF
A-11122 was used in Immunohistochemistry to indicate that GRP is a neuropeptide in sensory neurons for non-histaminergic itch, and GRP sensory neurons are dedicated to itch transmission.
Fri Mar 13 00:00:00 EDT 2020
A20 targets PFKL and glycolysis to inhibit the progression of hepatocellular carcinoma.
Cell death & disease
Feng Y,Zhang Y,Cai Y,Liu R,Lu M,Li T,Fu Y,Guo M,Huang H,Ou Y,Chen Y
A-11122 was used in Immunohistochemistry to establish A20 as a critical regulator of glycolysis and reveal a novel mechanism for A20 in tumor suppression and PFKL regulation.
Mon Feb 03 00:00:00 EST 2020
Changes in Wnt-Dependent Neuronal Morphology Underlie the Anatomical Diversification of Neocortical Homologs in Amniotes.
Cell reports
Nomura T,Ohtaka-Maruyama C,Kiyonari H,Gotoh H,Ono K
A-11122 was used in Immunocytochemistry to study the development of the six-layered mammalian neocortex in relation to the reptilian dorsal cortex.
Tue May 05 00:00:00 EDT 2020
Hindbrain Double-Negative Feedback Mediates Palatability-Guided Food and Water Consumption.
Cell
Gong R,Xu S,Hermundstad A,Yu Y,Sternson SM
A-11122 was used in Immunohistochemistry to identify glutamatergic neurons in the peri-locus coeruleus (periLCVGLUT2 neurons) as a polysynaptic convergence node from separate energy-sensitive and hydration-sensitive cell populations.
Thu Sep 17 00:00:00 EDT 2020
Small heat shock proteins determine synapse number and neuronal activity during development.
PloS one
Santana E,de Los Reyes T,Casas-Tintó S
A-11122 was used in Immunohistochemistry-immunofluorescence to explore the role of two small-HSPs (sHSPs), sHSP23 and sHSP26 in synaptogenesis and neuronal activity.
Mon Aug 24 00:00:00 EDT 2020
Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration.
eLife
Chen Y,Kotian N,Aranjuez G,Chen L,Messer CL,Burtscher A,Sawant K,Ramel D,Wang X,McDonald JA
A-11122 was used in Immunohistochemistry-immunofluorescence to study Pp1 as a major regulator of collective cohesion and migration in border cells.
Tue May 05 00:00:00 EDT 2020
TERMINAL FLOWER 1-FD complex target genes and competition with FLOWERING LOCUS T.
Nature communications
Zhu Y,Klasfeld S,Jeong CW,Jin R,Goto K,Yamaguchi N,Wagner D
A-11122 was used in Chromatin immunoprecipitation to provide mechanistic insight into how TFL1-FD sculpt inflorescence architecture, a trait important for reproductive success, plant architecture and yield.
Mon Oct 12 00:00:00 EDT 2020
Structural characterization of a novel human adeno-associated virus capsid with neurotropic properties.
Nature communications
Hsu HL,Brown A,Loveland AB,Lotun A,Xu M,Luo L,Xu G,Li J,Ren L,Su Q,Gessler DJ,Wei Y,Tai PWL,Korostelev AA,Gao G
A-11122 was used in Immunohistochemistry (Frozen) to describe a novel capsid identified in a human clinical sample by high-throughput, long-read sequencing.
Tue Jun 30 00:00:00 EDT 2020
A miRNA screen procedure identifies garz as an essential factor in adult glia functions and validates Drosophila as a beneficial 3Rs model to study glial functions and GBF1 biology.
F1000Research
Gonçalves-Pimentel C,Mazaud D,Kottler B,Proelss S,Hirth F,Fanto M
A-11122 was used in Immunocytochemistry to establish that garz is necessary in many glial cell types, that it affects motor behaviour and, at the sub-cellular level, is responsible for defects in cellular membranes, autophagy and mitochondria quality control.
Mon Mar 15 00:00:00 EDT 2021
Heterogeneous Presynaptic Distribution of Munc13 Isoforms at Retinal Synapses and Identification of an Unconventional Bipolar Cell Type with Dual Expression of Munc13 Isoforms: A Study Using Munc13-EXFP Knock-in Mice.
International journal of molecular sciences
Gierke K,von Wittgenstein J,Hemmerlein M,Atorf J,Joachimsthaler A,Kremers J,Cooper BH,Varoqueaux F,Regus-Leidig H,Brandstätter JH
A-11122 was used in Immunocytochemistry-immunoflourescence to use knock-in Munc13-EXFP mice to suggest that retinal synapses operate with a single Munc13 isoform, however type 6 ON bipolar cells express two Munc13 isoforms in their synaptic terminals.
Thu Oct 22 00:00:00 EDT 2020
Adaptive selection drives TRPP3 loss-of-function in an Ethiopian population.
Scientific reports
Walsh S,Izquierdo-Serra M,Acosta S,Edo A,Lloret M,Moret R,Bosch E,Oliva B,Bertranpetit J,Fernández-Fernández JM
A-11122 was used in Immunocytochemistry to describe the functional consequences of two non-synonymous genetic variants (R278Q and R378W) found to be under strong positive selection in an Ethiopian population, the Gumuz.
Wed Dec 02 00:00:00 EST 2020
Social Behavioral Deficits with Loss of Neurofibromin Emerge from Peripheral Chemosensory Neuron Dysfunction.
Cell reports
Moscato EH,Dubowy C,Walker JA,Kayser MS
A-11122 was used in Immunohistochemistry to identify a specific circuit mechanism through which Nf1 regulates social behaviors and suggest social deficits in NF1 arise from propagation of sensory misinformation.
Tue Jul 07 00:00:00 EDT 2020
Systematic elucidation of neuron-astrocyte interaction in models of amyotrophic lateral sclerosis using multi-modal integrated bioinformatics workflow.
Nature communications
Mishra V,Re DB,Le Verche V,Alvarez MJ,Vasciaveo A,Jacquier A,Doulias PT,Greco TM,Nizzardo M,Papadimitriou D,Nagata T,Rinchetti P,Perez-Torres EJ,Politi KA,Ikiz B,Clare K,Than ME,Corti S,Ischiropoulos H,Lotti F,Califano A,Przedborski S
A-11122 was used in Immunocytochemistry-immunoflourescence to support the usefulness of integrative, systems biology approach to gain insights into complex neurobiological disease processes as in ALS and posit that the proposed methodology is not restricted to this biological context and could be used in a variety of other non-cell-autonomous communication mechanisms.
Wed Nov 04 00:00:00 EST 2020
Innate immune signaling in Drosophila shifts anabolic lipid metabolism from triglyceride storage to phospholipid synthesis to support immune function.
PLoS genetics
Martínez BA,Hoyle RG,Yeudall S,Granade ME,Harris TE,Castle JD,Leitinger N,Bland ML
A-11122 was used in Western Blot to establish that Toll signaling induces a shift in anabolic lipid metabolism to favor phospholipid synthesis and ER expansion that may serve the immediate demand for AMP synthesis and secretion but with the long-term consequence of insufficient nutrient storage.
Sun Nov 01 00:00:00 EDT 2020
Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion.
Scientific reports
Barbaux S,Ialy-Radio C,Chalbi M,Dybal E,Homps-Legrand M,Do Cruzeiro M,Vaiman D,Wolf JP,Ziyyat A
A-11122 was used in Western Blot to propose a model in which IZUMO1 and SPACA6 would be part of a molecular complex necessary for gamete fusion and that their concomitant presence would be required for the recruitment of another essential molecular actor, such as a fusogen, for the fusion to take place.
Tue Mar 24 00:00:00 EDT 2020
Forebrain delta opioid receptors regulate the response of delta agonist in models of migraine and opioid-induced hyperalgesia.
Scientific reports
Dripps IJ,Bertels Z,Moye LS,Tipton AF,Siegersma K,Baca SM,Kieffer BL,Pradhan AA
A-11122 was used in Immunohistochemistry to indicate that forebrain DORs are necessary for the action of DOR agonists in relieving headache-related symptoms and suggest that forebrain regions may play an important role in migraine modulation.
Mon Oct 19 00:00:00 EDT 2020
Lateral Hypothalamic GABAergic Neurons Encode and Potentiate Sucrose's Palatability.
Frontiers in neuroscience
Garcia A,Coss A,Luis-Islas J,Puron-Sierra L,Luna M,Villavicencio M,Gutierrez R
A-11122 was used in Immunohistochemistry to show that LHAVgat+ neurons increase the drive to consume, but this is potentiated by the palatability and proximity of the tastant.
Wed Feb 10 00:00:00 EST 2021
CFAP53 regulates mammalian cilia-type motility patterns through differential localization and recruitment of axonemal dynein components.
PLoS genetics
Ide T,Twan WK,Lu H,Ikawa Y,Lim LX,Henninger N,Nishimura H,Takaoka K,Narasimhan V,Yan X,Shiratori H,Roy S,Hamada H
A-11122 was used in Immunohistochemistry-immunofluorescence to establish how differential localization and function of CFAP53 contributes to the unique motion patterns of two important mammalian cilia-types.
Tue Dec 01 00:00:00 EST 2020
Conservation and divergence of related neuronal lineages in the Drosophila central brain.
eLife
Lee YJ,Yang CP,Miyares RL,Huang YF,He Y,Ren Q,Chen HM,Kawase T,Ito M,Otsuna H,Sugino K,Aso Y,Ito K,Lee T
A-11122 was used in Immunohistochemistry-immunofluorescence to investigate how lineage identity translates to neuron morphology in Drosophila central brain lineages.
Tue Apr 07 00:00:00 EDT 2020
Non-cell autonomous promotion of astrogenesis at late embryonic stages by constitutive YAP activation.
Scientific reports
Han D,Kwon M,Lee SM,Pleasure SJ,Yoon K
A-11122 was used in Immunocytochemistry-Immunofluorescence experiments to show that constitutive YAP activation by in utero introduction of a non-degradable form of the YAP gene (YAP 5SA) causes productive GFAP+ cell generation at late embryonic periods, and this activity is nuclear localization- and TEAD transcription factor-dependent.
Mon Apr 27 00:00:00 EDT 2020
Non-cell autonomous promotion of astrogenesis at late embryonic stages by constitutive YAP activation.
Scientific reports
Han D,Kwon M,Lee SM,Pleasure SJ,Yoon K
A-11122 was used in Immunocytochemistry-Immunofluorescence experiments to show that constitutive YAP activation by in utero introduction of a non-degradable form of the YAP gene (YAP 5SA) causes productive GFAP+ cell generation at late embryonic periods, and this activity is nuclear localization- and TEAD transcription factor-dependent.
Mon Apr 27 00:00:00 EDT 2020
Behavioral effects of SGK1 knockout in VTA and dopamine neurons.
Scientific reports
Doyle MA,Stark AR,Fejes-Tóth G,Náray-Fejes-Tóth A,Mazei-Robison MS
A-11122 was used in Western Blotting to investigate the effect of SGK1 knockout in the ventral tegmental area (VTA) and in dopamine (DA) neurons to evaluate the necessity of protein expression for natural and drug reward behaviors.
Tue Sep 08 00:00:00 EDT 2020
Distinct fate, dynamics and niches of renal macrophages of bone marrow or embryonic origins.
Nature communications
Liu F,Dai S,Feng D,Qin Z,Peng X,Sakamuri SSVP,Ren M,Huang L,Cheng M,Mohammad KE,Qu P,Chen Y,Zhao C,Zhu F,Liang S,Aktas BH,Yang X,Wang H,Katakam PVG,Busija DW,Fischer T,Datta PK,Rappaport J,Gao B,Qin X
A-11122 was used in Immunocytochemistry-immunoflourescence to characterize renal macrophages at different stages in several transgenic lines.
Fri May 08 00:00:00 EDT 2020
The nanophthalmos protein TMEM98 inhibits MYRF self-cleavage and is required for eye size specification.
PLoS genetics
Cross SH,Mckie L,Hurd TW,Riley S,Wills J,Barnard AR,Young F,MacLaren RE,Jackson IJ
A-11122 was used in Immunohistochemistry to show eye size is dependent on a crucial interplay between TMEM98 and MYRF.
Wed Apr 01 00:00:00 EDT 2020
Adolescent frontal top-down neurons receive heightened local drive to establish adult attentional behavior in mice.
Nature communications
Nabel EM,Garkun Y,Koike H,Sadahiro M,Liang A,Norman KJ,Taccheri G,Demars MP,Im S,Caro K,Lopez S,Bateh J,Hof PR,Clem RL,Morishita H
A-11122 was used in Immunohistochemistry-immunofluorescence to reveal an adolescent sensitive period when top-down neurons integrate local circuits with long-range connectivity to produce attentional behavior.
Fri Aug 07 00:00:00 EDT 2020
A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress.
eLife
Stephani M,Picchianti L,Gajic A,Beveridge R,Skarwan E,Sanchez de Medina Hernandez V,Mohseni A,Clavel M,Zeng Y,Naumann C,Matuszkiewicz M,Turco E,Loefke C,Li B,Dürnberger G,Schutzbier M,Chen HT,Abdrakhmanov A,Savova A,Chia KS,Djamei A,Schaffner I,Abel S,Jiang L,Mechtler K,Ikeda F,Martens S,Clausen T,Dagdas Y
A-11122 was used in Immunohistochemistry to discover that C53 forms an ancient quality control pathway that bridges selective autophagy with ribosome-associated quality control in the endoplasmic reticulum.
Thu Aug 27 00:00:00 EDT 2020
Efficient Strategies for Microglia Replacement in the Central Nervous System.
Cell reports
Xu Z,Rao Y,Huang Y,Zhou T,Feng R,Xiong S,Yuan TF,Qin S,Lu Y,Zhou X,Li X,Qin B,Mao Y,Peng B
A-11122 was used in Immunohistochemistry to develop three efficient strategies for microglia replacement: microglia replacement by bone marrow transplantation (mrBMT), microglia replacement by peripheral blood (mrPB), and microglia replacement by microglia transplantation (mrMT).
Tue Aug 11 00:00:00 EDT 2020
A fluorescence-activatable reporter of flavivirus NS2B-NS3 protease activity enables live imaging of infection in single cells and viral plaques.
The Journal of biological chemistry
Arias-Arias JL,MacPherson DJ,Hill ME,Hardy JA,Mora-Rodríguez R
A-11122 was used in Western Blotting to report the construction of fluorescence-activatable sensors to detect the activities of flavivirus NS2B-NS3 serine proteases in living cells.
Fri Feb 21 00:00:00 EST 2020
Omega-3 Polyunsaturated Fatty Acids Inhibit the Function of Human URAT1, a Renal Urate Re-Absorber.
Nutrients
Saito H,Toyoda Y,Takada T,Hirata H,Ota-Kontani A,Miyata H,Kobayashi N,Tsuchiya Y,Suzuki H
A-11122 was used in Western Blotting to investigate the URAT1-inhibitory effects of 25 FAs that are commonly contained in foods or produced in the body.
Fri May 29 00:00:00 EDT 2020
Investigation of Isoform Specific Functions of the V-ATPase a Subunit During Drosophila Wing Development.
Frontiers in genetics
Mo D,Chen Y,Jiang N,Shen J,Zhang J
A-11122 was used in Immunohistochemistry-immunofluorescence to investigate the roles and functional diversity of V-ATPase subunits within endocytosis, protein degradation, fluid secretion and neurotransmission using a drosophila model.
Mon Sep 28 00:00:00 EDT 2020
Controlling motor neurons of every muscle for fly proboscis reaching.
eLife
McKellar CE,Siwanowicz I,Dickson BJ,Simpson JH
A-11122 was used in Immunohistochemistry to investigate the anatomy of all the primary motor neurons in the fly proboscis and characterise their contributions to its diverse reaching movements.
Thu Jun 25 00:00:00 EDT 2020
The Isolation of Stress Granules From Plant Material.
Current protocols in plant biology
Kosmacz M,Skirycz A
A-11122 was used in Immunopurification to describe an isolation protocol of stress granules that allows for the identification of proteins, mRNA, and metabolites sequestered into stress granules cores.
Tue Sep 01 00:00:00 EDT 2020
Distinct Dorsal and Ventral Hippocampal CA3 Outputs Govern Contextual Fear Discrimination.
Cell reports
Besnard A,Miller SM,Sahay A
A-11122 was used in Immunohistochemistry to identify opposing roles for dorsal CA3-CA1 (dCA3-dCA1) projections and dorsal CA3-dorsolateral septum (dCA3-DLS) projections in calibrating fear responses to certain and ambiguous contextual threats, respectively.
Tue Feb 18 00:00:00 EST 2020
Assessing the origin of high-grade serous ovarian cancer using CRISPR-modification of mouse organoids.
Nature communications
Lõhmussaar K,Kopper O,Korving J,Begthel H,Vreuls CPH,van Es JH,Clevers H
A-11122 was used in Immunohistochemistry-immunofluorescence to show in vitro drug testing reveals distinct lineage-dependent sensitivities to the common drugs used to treat HG-SOC in patients.
Wed May 27 00:00:00 EDT 2020
In vivo-directed evolution of adeno-associated virus in the primate retina.
JCI insight
Byrne LC,Day TP,Visel M,Strazzeri JA,Fortuny C,Dalkara D,Merigan WH,Schaffer DV,Flannery JG
A-11122 was used in Immunohistochemistry to demonstrate vectors with redirected tropism to the outer retina lead to increased gene delivery efficiency to retinal cells.
Thu May 21 00:00:00 EDT 2020
Nucleoplasmic signals promote directed transmembrane protein import simultaneously via multiple channels of nuclear pores.
Nature communications
Mudumbi KC,Czapiewski R,Ruba A,Junod SL,Li Y,Luo W,Ngo C,Ospina V,Schirmer EC,Yang W
A-11122 was used in Western Blotting to suggest that peripheral channel transport is the default mechanism that was adapted in evolution to include aspects of receptor-mediated central channel transport for directed trafficking of certain membrane proteins.
Mon May 04 00:00:00 EDT 2020
Three-Dimensional Imaging and Gene Expression Analysis Upon Enzymatic Isolation of the Tongue Epithelium.
Frontiers in physiology
Meisel CT,Pagella P,Porcheri C,Mitsiadis TA
A-11122 was used in Immunocytochemistry to analyse the multicellular compartmentalisation of isolated tongue epithelium by three-dimensional fluorescent imaging and quantitative real time PCR.
Mon Sep 28 00:00:00 EDT 2020
SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation.
Cell reports
Ros O,Baudet S,Zagar Y,Loulier K,Roche F,Couvet S,Aghaie A,Atkins M,Louail A,Petit C,Metin C,Mechulam Y,Nicol X
A-11122 was used in Immunohistochemistry, Immunocytochemistry to investigate local calcium signaling in vivo and directly manipulate this second messenger for therapeutic use.
Tue Jul 21 00:00:00 EDT 2020
SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation.
Cell reports
Ros O,Baudet S,Zagar Y,Loulier K,Roche F,Couvet S,Aghaie A,Atkins M,Louail A,Petit C,Metin C,Mechulam Y,Nicol X
A-11122 was used in Immunohistochemistry, Immunocytochemistry to investigate local calcium signaling in vivo and directly manipulate this second messenger for therapeutic use.
Tue Jul 21 00:00:00 EDT 2020
Definition of constitutive and stage-enriched promoters in the rodent malaria parasite, Plasmodium yoelii.
Malaria journal
Bowman LM,Finger LE,Hart KJ,Lindner SE
A-11122 was used in Immunocytochemistry-immunoflourescence to indicate that achieving stage-specific effects, such as gene editing, is likely best done using a two-component system with independent promoter activities overlapping only in the intended life cycle stage.
Mon Nov 23 00:00:00 EST 2020
The nuclear pore primes recombination-dependent DNA synthesis at arrested forks by promoting SUMO removal.
Nature communications
Kramarz K,Schirmeisen K,Boucherit V,Ait Saada A,Lovo C,Palancade B,Freudenreich C,Lambert SAE
A-11122 was used in Chromatin immunoprecipitation to establish a novel spatial control of Recombination-Dependent Replication (RDR) at a unique sequence that is distinct from mechanisms engaged at collapsed-forks and breaks within repeated sequences.
Fri Nov 06 00:00:00 EST 2020
Upregulation of nAChRs and Changes in Excitability on VTA Dopamine and GABA Neurons Correlates to Changes in Nicotine-Reward-Related Behavior.
eNeuro
Akers AT,Cooper SY,Baumgard ZJ,Casinelli GP,Avelar AJ,Henderson BJ
A-11122 was used in Immunocytochemistry to show nicotine reward-related behavior is linked to nicotinic acetylcholine receptors upregulation and neuron firing frequency.
Fri Jun 18 00:00:00 EDT 2021
Light microscopy of proteins in their ultrastructural context.
Nature communications
M'Saad O,Bewersdorf J
A-11122 was used in Immunocytochemistry-immunoflourescence to report the discovery of a new principle for an optical contrast equivalent to electron microscopy (EM) which reveals the ultrastructural context of the cells with a conventional confocal microscope.
Fri Jul 31 00:00:00 EDT 2020
Propionic acid induces dendritic spine loss by MAPK/ERK signaling and dysregulation of autophagic flux.
Molecular brain
Choi H,Kim IS,Mun JY
A-11122 was used in Immunocytochemistry to identify a link between PPA and autism phenotypes facilitated by signaling mechanisms in hippocampal neurons.
Tue Jun 02 00:00:00 EDT 2020
Smchd1 is a maternal effect gene required for genomic imprinting.
eLife
Wanigasuriya I,Gouil Q,Kinkel SA,Tapia Del Fierro A,Beck T,Roper EA,Breslin K,Stringer J,Hutt K,Lee HJ,Keniry A,Ritchie ME,Blewitt ME
A-11122 was used in Immunocytochemistry, ChIP assay to report epigenetic repressor Smchd1 as a novel maternal effect gene that regulates the imprinted expression of ten genes in mice.
Fri Nov 13 00:00:00 EST 2020
Smchd1 is a maternal effect gene required for genomic imprinting.
eLife
Wanigasuriya I,Gouil Q,Kinkel SA,Tapia Del Fierro A,Beck T,Roper EA,Breslin K,Stringer J,Hutt K,Lee HJ,Keniry A,Ritchie ME,Blewitt ME
A-11122 was used in Immunocytochemistry, ChIP assay to report epigenetic repressor Smchd1 as a novel maternal effect gene that regulates the imprinted expression of ten genes in mice.
Fri Nov 13 00:00:00 EST 2020
Comparison of AAV-Mediated Optogenetic Vision Restoration between Retinal Ganglion Cell Expression and ON Bipolar Cell Targeting.
Molecular therapy. Methods & clinical development
Lu Q,Ganjawala TH,Krstevski A,Abrams GW,Pan ZH
A-11122 was used in Western Blotting to compare the restored visual functions achieved by adeno-associated virus (AAV)-mediated expression of a channelrhodopsin in ON-type bipolar cells and retinal ganglion cells driven by an improved mGluR6 promoter and a CAG promoter, respectively, in a blind mouse model by performing electrophysiological recordings and behavioral assessments.
Fri Sep 11 00:00:00 EDT 2020
A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1.
eLife
Felipe-Medina N,Caburet S,Sánchez-Sáez F,Condezo YB,de Rooij DG,Gómez-H L,Garcia-Valiente R,Todeschini AL,Duque P,Sánchez-Martin MA,Shalev SA,Llano E,Veitia RA,Pendás AM
A-11122 was used in Western Blotting to investigate the molecular mechanism of essential meiotic variant HSF2BP-S167L underlying human ovarian insufficiency and sub-fertility.
Wed Aug 26 00:00:00 EDT 2020
Protective role of neuronal and lymphoid cannabinoid CB2 receptors in neuropathic pain.
eLife
Cabañero D,Ramírez-López A,Drews E,Schmöle A,Otte DM,Wawrzczak-Bargiela A,Huerga Encabo H,Kummer S,Ferrer-Montiel A,Przewlocki R,Zimmer A,Maldonado R
A-11122 was used in Immunohistochemistry (Frozen) to study the role of the cannabinoid 2 receptor in regulating spontaneous neuropathic pain through allowing self administration of a CB2 agonist in wildtype and CB2 knockout mice models.
Mon Jul 20 00:00:00 EDT 2020
Subnuclear gene positioning through lamina association affects copper tolerance.
Nature communications
Sakamoto Y,Sato M,Sato Y,Harada A,Suzuki T,Goto C,Tamura K,Toyooka K,Kimura H,Ohkawa Y,Hara-Nishimura I,Takagi S,Matsunaga S
A-11122 was used in Immunomicroscopy to study the biological function of CROWDED NUCLEI in Arabidopsis thaliana.
Fri Nov 20 00:00:00 EST 2020
Anandamide Metabolites Protect against Seizures through the TRP Channel Water Witch in Drosophila melanogaster.
Cell reports
Jacobs JA,Sehgal A
A-11122 was used in Immunohistochemistry to reveal the role of arachidonic acid in seizure protection and identify a cannabinoid-receptor-1/2-independent mechanism of endocannabinoid seizure protection.
Tue Jun 02 00:00:00 EDT 2020
The epidermis coordinates thermoresponsive growth through the phyB-PIF4-auxin pathway.
Nature communications
Kim S,Hwang G,Kim S,Thi TN,Kim H,Jeong J,Kim J,Kim J,Choi G,Oh E
A-11122 was used in Chromatin immunoprecipitation to show that epidermis-specific expression of PIF4 induces constitutive long hypocotyls, while vasculature-specific expression of PIF4 has no effect on hypocotyl growth.
Wed Feb 26 00:00:00 EST 2020
TRIM11 Prevents and Reverses Protein Aggregation and Rescues a Mouse Model of Parkinson's Disease.
Cell reports
Zhu G,Harischandra DS,Ghaisas S,Zhang P,Prall W,Huang L,Maghames C,Guo L,Luna E,Mack KL,Torrente MP,Luk KC,Shorter J,Yang X
A-11122 was used in Immunohistochemistry to define TRIMs as a potent and multifunctional protein quality-control system in metazoa, which might be applied to treat neurodegenerative diseases.
Tue Dec 01 00:00:00 EST 2020
The Warburg Effect and lactate signaling augment Fgf-MAPK to promote sensory-neural development in the otic vesicle.
eLife
Kantarci H,Gou Y,Riley BB
A-11122 was used in Immunohistochemistry to demonstrate that disrupting the glycolytic gene phosphoglycerate kinase-1 (pgk1) impairs Fgf-dependent development of hair cells and neurons in the otic vesicle and other neurons in the CNS/PNS.
Mon Apr 27 00:00:00 EDT 2020
Decapentaplegic signaling regulates Wingless ligand production and target activation during Drosophila wing development.
FEBS letters
Li Y,Zhang F,Jiang N,Liu T,Shen J,Zhang J
A-11122 was used in Immunohistochemistry to support a model in which decapentaplegic (Dpp) signaling plays a dual role in transcriptional regulation of both the wingless (wg) gene and downstream targets.
Wed Apr 01 00:00:00 EDT 2020
Age-Dependent Activation and Neuronal Differentiation of Lgr5+ Basal Cells in Injured Olfactory Epithelium via Notch Signaling Pathway.
Frontiers in aging neuroscience
Li X,Tong M,Wang L,Qin Y,Yu H,Yu Y
A-11122 was used in Immunocytochemistry-immunofluorescence to show that activation of Lgr5+ cells in injured OE was age dependent and Notch activation could enhance the capacity of neuronal generation from Lgr5+ cells in aged OE after injury.
Tue Jan 05 00:00:00 EST 2021
The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells.
Nature communications
Zhang J,Gurusaran M,Fujiwara Y,Zhang K,Echbarthi M,Vorontsov E,Guo R,Pendlebury DF,Alam I,Livera G,Emmanuelle M,Wang PJ,Nandakumar J,Davies OR,Shibuya H
A-11122 was used in Immunoprecipitation to identify BRCA2 and MEILB2-associating protein 1 (BRME1), which functions as a stabilizer of MEILB2 by binding to an α-helical N-terminus of MEILB2 and preventing MEILB2 self-association.
Tue Apr 28 00:00:00 EDT 2020
Identification of a molecular basis for the juvenile sleep state.
eLife
Chakravarti Dilley L,Szuperak M,Gong NN,Williams CE,Saldana RL,Garbe DS,Syed MH,Jain R,Kayser MS
A-11122 was used in Immunohistochemistry to define the molecular cues governing sleep behavioral and circuit development, and suggest sleep disorders may be of neurodevelopmental origin.
Mon Mar 23 00:00:00 EDT 2020
A Brainstem Locomotor Circuit Drives the Activity of Speed Cells in the Medial Entorhinal Cortex.
Cell reports
Carvalho MM,Tanke N,Kropff E,Witter MP,Moser MB,Moser EI
A-11122 was used in Immunohistochemistry to provide evidence for a pathway by which brainstem speed signals can reach cortical structures implicated in navigation and higher-order dynamic representations of space.
Tue Sep 08 00:00:00 EDT 2020
Upregulated TNF/Eiger signaling mediates stem cell recovery and tissue homeostasis during nutrient resupply in Drosophila testis.
Scientific reports
Chang YC,Tu H,Huang TW,Xu BW,Pi H
A-11122 was used in Immunohistochemistry to show that tumor necrosis factor Eiger (Egr) is upregulated in testicular smooth muscles as a response to prolonged protein starvation in Drosophila testis.
Wed Jul 15 00:00:00 EDT 2020
Exercise enhances motor skill learning by neurotransmitter switching in the adult midbrain.
Nature communications
Li HQ,Spitzer NC
A-11122 was used in Immunohistochemistry-immunofluorescence to suggest that neurotransmitter switching provides the basis by which sustained running benefits motor skill learning, presenting a target for clinical treatment of movement disorders.
Mon May 04 00:00:00 EDT 2020
Cortical anchoring of the microtubule cytoskeleton is essential for neuron polarity.
eLife
He L,Kooistra R,Das R,Oudejans E,van Leen E,Ziegler J,Portegies S,de Haan B,van Regteren Altena A,Stucchi R,Altelaar AM,Wieser S,Krieg M,Hoogenraad CC,Harterink M
A-11122 was used in Immunocytochemistry-immunoflourescence to study how organization of the microtubule cytoskeleton is established and maintained.
Wed Apr 15 00:00:00 EDT 2020
Mutations associated with human neural tube defects display disrupted planar cell polarity in Drosophila.
eLife
Humphries AC,Narang S,Mlodzik M
A-11122 was used in Immunohistochemistry-immunofluorescence to provide mechanistic insight into how different mammalian mutations contribute to developmental disorders and strengthen the link between PCP and NTD.
Wed Apr 01 00:00:00 EDT 2020
The dynamic interplay between ATP/ADP levels and autophagy sustain neuronal migration in vivo.
eLife
Bressan C,Pecora A,Gagnon D,Snapyan M,Labrecque S,De Koninck P,Parent M,Saghatelyan A
A-11122 was used in Immunohistochemistry-immunofluorescence to show that autophagy and energy consumption act in concert in migrating cells to dynamically regulate the pace and periodicity of the migratory and stationary phases to sustain neuronal migration.
Mon Sep 28 00:00:00 EDT 2020
Coordinated crosstalk between microtubules and actin by a spectraplakin regulates lumen formation and branching.
eLife
Ricolo D,Araujo SJ
A-11122 was used in Immunohistochemistry to provide the first evidence for a role for spectraplakins in single-cell lumen formation and branching.
Wed Oct 28 00:00:00 EDT 2020
The Rho-GEF PIX-1 directs assembly or stability of lateral attachment structures between muscle cells.
Nature communications
Moody JC,Qadota H,Reedy AR,Okafor CD,Shanmugan N,Matsunaga Y,Christian CJ,Ortlund EA,Benian GM
A-11122 was used in Immunocytochemistry to show that a PIX protein has an important function in muscle.
Tue Oct 06 00:00:00 EDT 2020
Neurochemical Organization of the Drosophila Brain Visualized by Endogenously Tagged Neurotransmitter Receptors.
Cell reports
Kondo S,Takahashi T,Yamagata N,Imanishi Y,Katow H,Hiramatsu S,Lynn K,Abe A,Kumaraswamy A,Tanimoto H
A-11122 was used in Immunohistochemistry to we profile the expression of 75 neurotransmitter receptors in the brain, to reveal neurochemical segmentation.
Tue Jan 07 00:00:00 EST 2020
DNMT3A Haploinsufficiency Results in Behavioral Deficits and Global Epigenomic Dysregulation Shared across Neurodevelopmental Disorders.
Cell reports
Christian DL,Wu DY,Martin JR,Moore JR,Liu YR,Clemens AW,Nettles SA,Kirkland NM,Papouin T,Hill CA,Wozniak DF,Dougherty JD,Gabel HW
A-11122 was used in Western Blotting to investigate the effects of DNA methyltransferase 3A mutations when associated with neurodevelopmental disease.
Tue Nov 24 00:00:00 EST 2020
Plasmodium DEH is ER-localized and crucial for oocyst mitotic division during malaria transmission.
Life science alliance
Guttery DS,Pandey R,Ferguson DJ,Wall RJ,Brady D,Gupta D,Holder AA,Tewari R
A-11122 was used in Western Blotting to characterized in-depth the location and function of DEH, applying in silico analysis, live cell imaging, reverse genetics, and ultrastructure analysis using the mouse malaria model Plasmodium berghei.
Tue Dec 01 00:00:00 EST 2020
An Interaction Network of RNA-Binding Proteins Involved in Drosophila Oogenesis.
Molecular & cellular proteomics : MCP
Bansal P,Madlung J,Schaaf K,Macek B,Bono F
A-11122 was used in Western Blotting to examine protein-protein associations of 6 GFP-tagged RBPs expressed at physiological levels, using immunoprecipitation from whole Drosophila ovaries coupled to mass spectrometry.
Tue Sep 01 00:00:00 EDT 2020
Removal of H2Aub1 by ubiquitin-specific proteases 12 and 13 is required for stable Polycomb-mediated gene repression in Arabidopsis.
Genome biology
Kralemann LEM,Liu S,Trejo-Arellano MS,Muñoz-Viana R,Köhler C,Hennig L
A-11122 was used in Chromatin immunoprecipitation to investigate the collaborative nature of UBP12/13, PRC1/2 and EMF1 in repressing genes involved within the response to stimuli within arabidopsis.
Tue Jun 16 00:00:00 EDT 2020
Cell types and neuronal circuitry underlying female aggression in Drosophila.
eLife
Schretter CE,Aso Y,Robie AA,Dreher M,Dolan MJ,Chen N,Ito M,Yang T,Parekh R,Branson KM,Rubin GM
A-11122 was used in Immunohistochemistry-immunofluorescence to reveal important regulatory components of the neuronal circuitry that underlies female aggressive social interactions and provides tools for their manipulation.
Tue Nov 03 00:00:00 EST 2020
Wiskott-Aldrich syndrome protein restricts cGAS/STING activation by dsDNA immune complexes.
JCI insight
Piperno GM,Naseem A,Silvestrelli G,Amadio R,Caronni N,Cervantes-Luevano KE,Liv N,Klumperman J,Colliva A,Ali H,Graziano F,Benaroch P,Haecker H,Hanna RN,Benvenuti F
A-11122 was used in Immunocytochemistry-immunoflourescence to show that immune complexes of self-DNA and autoantibodies (DNA-ICs) contribute to elevated IFN levels via activation of the cGAS/STING pathway of cytosolic sensing.
Thu Sep 03 00:00:00 EDT 2020
Inhibitory Interactions and Columnar Inputs to an Object Motion Detector in Drosophila.
Cell reports
Keleş MF,Hardcastle BJ,Städele C,Xiao Q,Frye MA
A-11122 was used in Immunohistochemistry-immunofluorescence to study a highly specialized small object motion detector, LC11, and demonstrate that its responses are suppressed by local background motion.
Tue Feb 18 00:00:00 EST 2020
Autophagy Activation in Zebrafish Heart Regeneration.
Scientific reports
Chávez MN,Morales RA,López-Crisosto C,Roa JC,Allende ML,Lavandero S
A-11122 was used in Immunohistochemistry to show that a tightly regulated autophagic response is triggered upon injury and during the early stages of the regeneration process.
Mon Feb 10 00:00:00 EST 2020
Latrophilin-2 and latrophilin-3 are redundantly essential for parallel-fiber synapse function in cerebellum.
eLife
Zhang RS,Liakath-Ali K,Südhof TC
A-11122 was used in Western Blot to demonstrate that Lphn2 and Lphn3 are selectively but redundantly required in Purkinje cells for parallel-fiber synapses.
Mon Mar 23 00:00:00 EDT 2020
Down-regulation of Beclin1 promotes direct cardiac reprogramming.
Science translational medicine
Wang L,Ma H,Huang P,Xie Y,Near D,Wang H,Xu J,Yang Y,Xu Y,Garbutt T,Zhou Y,Liu Z,Yin C,Bressan M,Taylor JM,Liu J,Qian L
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, Flow Cytometry to reveal an inductive role of Atg5-dependent autophagy as well as a previously unrecognized autophagy-independent inhibitory function of Becn1 in iCM reprogramming.
Wed Oct 21 00:00:00 EDT 2020
Immunopurification of Intact Endosomal Compartments for Lipid Analyses in Arabidopsis.
Methods in molecular biology (Clifton, N.J.)
Ito Y,Grison M,Esnay N,Fouillen L,Boutté Y
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation to describe and discuss the immunoprecipitation protocol to isolate endosomes with the aim to analyze lipid content.
Thu Mar 18 00:00:00 EDT 2021
Exercise rejuvenates quiescent skeletal muscle stem cells in old mice through restoration of Cyclin D1.
Nature metabolism
Brett JO,Arjona M,Ikeda M,Quarta M,de Morrée A,Egner IM,Perandini LA,Ishak HD,Goshayeshi A,Benjamin DI,Both P,Rodríguez-Mateo C,Betley MJ,Wyss-Coray T,Rando TA
A-11122 was used in Immunohistochemistry to investigate the effect of exercise on aged stem cell function in the context of tissue regeneration in old mice.
Wed Apr 01 00:00:00 EDT 2020
Conditional Single Vector CRISPR/SaCas9 Viruses for Efficient Mutagenesis in the Adult Mouse Nervous System.
Cell reports
Hunker AC,Soden ME,Krayushkina D,Heymann G,Awatramani R,Zweifel LS
A-11122 was used in Immunohistochemistry to reveal insight into the role of GABAergic regulation of midbrain GABA-producing neurons in psychomotor activation.
Tue Mar 24 00:00:00 EDT 2020
dnmt1 function is required to maintain retinal stem cells within the ciliary marginal zone of the zebrafish eye.
Scientific reports
Angileri KM,Gross JM
A-11122 was used in Immunohistochemistry to demonstrate that zebrafish dnmt1s872 mutants possess severe defects in RSC maintenance within the ciliary marginal zone (CMZ).
Thu Jul 09 00:00:00 EDT 2020
Integration of Nodal and BMP Signaling by Mutual Signaling Effector Antagonism.
Cell reports
Soh GH,Pomreinke AP,Müller P
A-11122 was used in Western Blotting to show that Smad2 and Smad5 antagonize each other for specific cell fates, providing a mechanism for how cells integrate and discriminate between overlapping signals during development.
Tue Apr 07 00:00:00 EDT 2020
Tp53 Suppression Promotes Cardiomyocyte Proliferation during Zebrafish Heart Regeneration.
Cell reports
Shoffner A,Cigliola V,Lee N,Ou J,Poss KD
A-11122 was used in Immunohistochemistry-immunofluorescence to show that myocardial Tp53 suppression by mitogen-induced Mdm2 is a regulatory component of innate cardiac regeneration.
Tue Sep 01 00:00:00 EDT 2020
Different functions of two putative Drosophila α2δ subunits in the same identified motoneurons.
Scientific reports
Heinrich L,Ryglewski S
A-11122 was used in Western Blotting to suggest that the two different putative α2δ subunits are required in the same neurons to regulate different functions of voltage gated calcium channels.
Thu Aug 13 00:00:00 EDT 2020
Mitochondrial residence of the apoptosis inducer BAX is more important than BAX oligomerization in promoting membrane permeabilization.
The Journal of biological chemistry
Kuwana T,King LE,Cosentino K,Suess J,Garcia-Saez AJ,Gilmore AP,Newmeyer DD
A-11122 was used in Immunocytochemistry-immunoflourescence to conclude that BAX’s mitochondrial residence in vivo, regulated by both targeting and retrotranslocation, is more significant for its pro-apoptotic activity than its ability to insert and to form higher-order oligomers in model membranes.
Fri Feb 07 00:00:00 EST 2020
Distributed control of motor circuits for backward walking in Drosophila.
Nature communications
Feng K,Sen R,Minegishi R,Dübbert M,Bockemühl T,Büschges A,Dickson BJ
A-11122 was used in Immunohistochemistry to identify several dozen MDN target neurons within the leg motor circuits, and show that two of them mediate distinct and highly-specific changes in leg muscle activity during backward walking: LBL40 neurons provide the hindleg power stroke during stance phase; LUL130 neurons lift the legs at the end of stance to initiate swing.
Wed Dec 02 00:00:00 EST 2020
The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment.
eLife
Sanders SS,Hernandez LM,Soh H,Karnam S,Walikonis RS,Tzingounis AV,Thomas GM
A-11122 was used in Immunocytochemistry to identify the PDZ domain-containing Membrane-associated Guanylate Kinase (MaGUK) PSD93 as a direct ZDHHC14 interactor and substrate.
Fri Nov 13 00:00:00 EST 2020
Distinct subpopulations of mechanosensory chordotonal organ neurons elicit grooming of the fruit fly antennae.
eLife
Hampel S,Eichler K,Yamada D,Bock DD,Kamikouchi A,Seeds AM
A-11122 was used in Immunohistochemistry-immunofluorescence to show that the subpopulations project to different, discrete zones in the brain and differ in their responses to mechanical stimulation of the antennae.
Mon Oct 26 00:00:00 EDT 2020
Hox-dependent coordination of mouse cardiac progenitor cell patterning and differentiation.
eLife
Stefanovic S,Laforest B,Desvignes JP,Lescroart F,Argiro L,Maurel-Zaffran C,Salgado D,Plaindoux E,De Bono C,Pazur K,Théveniau-Ruissy M,Béroud C,Puceat M,Gavalas A,Kelly RG,Zaffran S
A-11122 was used in Immunocytochemistry to profile the transcriptome and chromatin accessibility of anterior and posterior SHF sub-populations at genome-wide levels and demonstrate that Hoxb1 negatively regulates differentiation in the posterior SHF.
Mon Aug 17 00:00:00 EDT 2020
Visual Experience Influences Dendritic Orientation but Is Not Required for Asymmetric Wiring of the Retinal Direction Selective Circuit.
Cell reports
El-Quessny M,Maanum K,Feller MB
A-11122 was used in Immunocytochemistry to indicate that different mechanisms of a cell's computational abilities can be constructed over development through divergent mechanisms.
Tue Jun 30 00:00:00 EDT 2020
Protein interactome of the Cancerous Inhibitor of protein phosphatase 2A (CIP2A) in Th17 cells.
Current research in immunology
Khan MM,Välikangas T,Khan MH,Moulder R,Ullah U,Bhosale SD,Komsi E,Butt U,Qiao X,Westermarck J,Elo LL,Lahesmaa R
A-11122 was used in Proximity Ligation Assay (PLA) to present the first systematic characterization of the CIP2A interactome by affinity-purification mass spectrometry combined with validation by selected reaction monitoring targeted mass spectrometry (SRM-MS) analysis in T helper (Th) 17 (Th17) cells.
Tue Dec 01 00:00:00 EST 2020
Inhibition of histone deacetylation rescues phenotype in a mouse model of Birk-Barel intellectual disability syndrome.
Nature communications
Cooper A,Butto T,Hammer N,Jagannath S,Fend-Guella DL,Akhtar J,Radyushkin K,Lesage F,Winter J,Strand S,Roeper J,Zechner U,Schweiger S
A-11122 was used in Immunohistochemistry to suggest a potential approach to improve cognitive dysfunction in a mouse model of an imprinting disorder.
Fri Jan 24 00:00:00 EST 2020
Cellular identity and Ca2+ signaling activity of the non-reproductive GnRH system in the Ciona intestinalis type A (Ciona robusta) larva.
Scientific reports
Okawa N,Shimai K,Ohnishi K,Ohkura M,Nakai J,Horie T,Kuhara A,Kusakabe TG
A-11122 was used in Immunocytochemistry to investigate the neuronal types of gnrh2-expressing cells in Ciona larvae and visualized the activity of these cells by fluorescence imaging using a calcium sensor protein.
Thu Oct 29 00:00:00 EDT 2020
Intravital three-dimensional bioprinting.
Nature biomedical engineering
Urciuolo A,Poli I,Brandolino L,Raffa P,Scattolini V,Laterza C,Giobbe GG,Zambaiti E,Selmin G,Magnussen M,Brigo L,De Coppi P,Salmaso S,Giomo M,Elvassore N
A-11122 was used in Immunohistochemistry to show that intravital 3D bioprinting of donor-muscle-derived stem cells under the epimysium of hindlimb muscle in mice leads to the de novo formation of myofibres in the mice.
Tue Sep 01 00:00:00 EDT 2020
Nestin-GFP transgene labels skeletal progenitors in the periosteum.
Bone
Tournaire G,Stegen S,Giacomini G,Stockmans I,Moermans K,Carmeliet G,van Gastel N
A-11122 was used in Flow Cytometry to show that in adult mice, Nes-GFP cells are present in the periosteum and localize closely to blood vessels, but periosteal Nes-GFP cells express SSC and progenitor markers differently compared to Nes-GFP cells in the bone marrow.
Wed Apr 01 00:00:00 EDT 2020
Genome analysis of sphingolipid metabolism-related genes in Tetrahymena thermophila and identification of a fatty acid 2-hydroxylase involved in the sexual stage of conjugation.
Molecular microbiology
Cid NG,Puca G,Nudel CB,Nusblat AD
A-11122 was used in Immunocytochemistry to identify the fatty acid 2-hydroxylase gene in the sphingolipid metabolic pathway.
Sun Nov 01 00:00:00 EDT 2020
Overlapping Activities of ELAV/Hu Family RNA Binding Proteins Specify the Extended Neuronal 3' UTR Landscape in Drosophila.
Molecular cell
Wei L,Lee S,Majumdar S,Zhang B,Sanfilippo P,Joseph B,Miura P,Soller M,Lai EC
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot to demonstrate how regulatory interplay and functionally overlapping activities of neural ELAV/Hu RBPs drives the neural APA landscape.
Thu Oct 01 00:00:00 EDT 2020
A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth.
Nature plants
Belda-Palazón B,Adamo M,Valerio C,Ferreira LJ,Confraria A,Reis-Barata D,Rodrigues A,Meyer C,Rodriguez PL,Baena-González E
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot to investigate SnRK2 and SnRK1 kinases in plant growth.
Sun Nov 01 00:00:00 EDT 2020
A glucose-sensing neuron pair regulates insulin and glucagon in Drosophila.
Nature
Oh Y,Lai JS,Mills HJ,Erdjument-Bromage H,Giammarinaro B,Saadipour K,Wang JG,Abu F,Neubert TA,Suh GSB
A-11122 was used in Immunohistochemistry to identify a pair of glucose-sensing neurons with bifurcated axons in the brain of Drosophila.
Tue Oct 01 00:00:00 EDT 2019
Identifying the pathways required for coping behaviours associated with sustained pain.
Nature
Huang T,Lin SH,Malewicz NM,Zhang Y,Zhang Y,Goulding M,LaMotte RH,Ma Q
A-11122 was used in Immunohistochemistry to reveals a fundamental subdivision within the cutaneous somatosensory system, and challenges the validity of using reflexive defensive responses to measure sustained pain.
Tue Jan 01 00:00:00 EST 2019
Effector TH17 Cells Give Rise to Long-Lived TRM Cells that Are Essential for an Immediate Response against Bacterial Infection.
Cell
Amezcua Vesely MC,Pallis P,Bielecki P,Low JS,Zhao J,Harman CCD,Kroehling L,Jackson R,Bailis W,Licona-Limón P,Xu H,Iijima N,Pillai PS,Kaplan DH,Weaver CT,Kluger Y,Kowalczyk MS,Iwasaki A,Pereira JP,Esplugues E,Gagliani N,Flavell RA
A-11122 was used in immunohistochemistry (frozen) immunofluorescence to delineate the origin and function of airway CD4 TRM cells during bacterial infection.
Thu Aug 22 00:00:00 EDT 2019
Neuromodulator Signaling Bidirectionally Controls Vesicle Numbers in Human Synapses.
Cell
Patzke C,Brockmann MM,Dai J,Gan KJ,Grauel MK,Fenske P,Liu Y,Acuna C,Rosenmund C,Südhof TC
A-11122 was used in Immunocytochemistry-immunoflourescence to characterize human and mouse synapses and reveal that neuromodulators impact synaptic transmission by altering synaptic vesicle numbers in presynaptic nerve terminals.
Thu Oct 03 00:00:00 EDT 2019
A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability.
Cell host & microbe
Wilke G,Funkhouser-Jones LJ,Wang Y,Ravindran S,Wang Q,Beatty WL,Baldridge MT,VanDussen KL,Shen B,Kuhlenschmidt MS,Kuhlenschmidt TB,Witola WH,Stappenbeck TS,Sibley LD
A-11122 was used in Immunohistochemistry-immunofluorescence to describe a platform for complete life cycle development and long-term growth of Cryptosporidium.
Wed Jul 10 00:00:00 EDT 2019
Divergent medial amygdala projections regulate approach-avoidance conflict behavior.
Nature neuroscience
Miller SM,Marcotulli D,Shen A,Zweifel LS
A-11122 was used in Immunohistochemistry to demonstrate divergent pathways in the MeApv that can be differentially weighted toward exploration or evasion of threats.
Mon Apr 01 00:00:00 EDT 2019
State-dependent decoupling of sensory and motor circuits underlies behavioral flexibility in Drosophila.
Nature neuroscience
Ache JM,Namiki S,Lee A,Branson K,Card GM
A-11122 was used in Immunohistochemistry to identify two descending neuron (DN) types that control landing and contribute to visuomotor flexibility in Drosophila.
Mon Jul 01 00:00:00 EDT 2019
Single-Cell Transcriptomics Uncovers Glial Progenitor Diversity and Cell Fate Determinants during Development and Gliomagenesis.
Cell stem cell
Weng Q,Wang J,Wang J,He D,Cheng Z,Zhang F,Verma R,Xu L,Dong X,Liao Y,He X,Potter A,Zhang L,Zhao C,Xin M,Zhou Q,Aronow BJ,Blackshear PJ,Rich JN,He Q,Zhou W,Suvà ML,Waclaw RR,Potter SS,Yu G,Lu QR
A-11122 was used in Immunohistochemistry-immunofluorescence to resolve the dynamic repertoire of common and divergent glial progenitors during development and tumorigenesis and highlight Zfp36l1 as a molecular nexus for balancing glial cell-fate decision and controlling gliomagenesis.
Thu May 02 00:00:00 EDT 2019
Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency.
Cell stem cell
Kalkan T,Bornelöv S,Mulas C,Diamanti E,Lohoff T,Ralser M,Middelkamp S,Lombard P,Nichols J,Smith A
A-11122 was used in Immunocytochemistry-immunoflourescence to show that genetic elimination of three complementary drivers of network transition stalls developmental progression, emulating environmental insulation by small-molecule inhibitors.
Thu May 02 00:00:00 EDT 2019
Single-Cell Transcriptomic Analyses of Cell Fate Transitions during Human Cardiac Reprogramming.
Cell stem cell
Zhou Y,Liu Z,Welch JD,Gao X,Wang L,Garbutt T,Keepers B,Ma H,Prins JF,Shen W,Liu J,Qian L
A-11122 was used in Immunocytochemistry-immunoflourescence to study the human cardiac reprogramming that utilizes an analysis pipeline and a cell fate index calculation we developed to measure reprogramming progression.
Wed Jul 03 00:00:00 EDT 2019
Single-Cell Transcriptomic Analyses of Cell Fate Transitions during Human Cardiac Reprogramming.
Cell stem cell
Zhou Y,Liu Z,Welch JD,Gao X,Wang L,Garbutt T,Keepers B,Ma H,Prins JF,Shen W,Liu J,Qian L
A-11122 was used in Immunocytochemistry-immunoflourescence to study the human cardiac reprogramming that utilizes an analysis pipeline and a cell fate index calculation we developed to measure reprogramming progression.
Wed Jul 03 00:00:00 EDT 2019
Cardiac Reprogramming Factors Synergistically Activate Genome-wide Cardiogenic Stage-Specific Enhancers.
Cell stem cell
Hashimoto H,Wang Z,Garry GA,Malladi VS,Botten GA,Ye W,Zhou H,Osterwalder M,Dickel DE,Visel A,Liu N,Bassel-Duby R,Olson EN
A-11122 was used in Immunocytochemistry to perform genome-wide analyses of cardiogenic TF binding and enhancer profiling during cardiac reprogramming.
Wed Jul 03 00:00:00 EDT 2019
Competition for Mitogens Regulates Spermatogenic Stem Cell Homeostasis in an Open Niche.
Cell stem cell
Kitadate Y,Jörg DJ,Tokue M,Maruyama A,Ichikawa R,Tsuchiya S,Segi-Nishida E,Nakagawa T,Uchida A,Kimura-Yoshida C,Mizuno S,Sugiyama F,Azami T,Ema M,Noda C,Kobayashi S,Matsuo I,Kanai Y,Nagasawa T,Sugimoto Y,Takahashi S,Simons BD,Yoshida S
A-11122 was used in Immunohistochemistry-immunofluorescence to demonstrate, using murine spermatogenesis as a model, that spermatogenic stem cell density is tightly regulated by competition for mitogens.
Thu Jan 03 00:00:00 EST 2019
Ultra-soft X-ray system for imaging the early cellular responses to X-ray induced DNA damage.
Nucleic acids research
Kochan JA,van den Belt M,von der Lippe J,Desclos ECB,Steurer B,Hoebe RA,Scutigliani EM,Verhoeven J,Stap J,Bosch R,Rijpkema M,van Oven C,van Veen HA,Stellingwerf I,Vriend LEM,Marteijn JA,Aten JA,Krawczyk PM
A-11122 was used in Western Blotting to reveal, for the first time, the kinetics of DSB signaling triggered by X-ray microirradiation and establish XM3 as a powerful platform for real-time analysis of cellular DSB repair responses.
Thu Sep 26 00:00:00 EDT 2019
SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice.
Nucleic acids research
Liu H,Huang T,Li M,Li M,Zhang C,Jiang J,Yu X,Yin Y,Zhang F,Lu G,Luo MC,Zhang LR,Li J,Liu K,Chen ZJ
A-11122 was used in Immunocytochemistry-immunoflourescence to identify a novel protein, synaptonemal complex reinforcing element as a key molecule in maintaining the integrity of synaptonemal complex during meiosis prophase 1 in mice.
Thu Jun 20 00:00:00 EDT 2019
Positional Strategies for Connection Specificity and Synaptic Organization in Spinal Sensory-Motor Circuits.
Neuron
Balaskas N,Abbott LF,Jessell TM,Ng D
A-11122 was used in Immunohistochemistry to propose positional strategies that could achieve sensory-motor connectivity and synaptic organisation in spinal sensory-motor circuits.
Wed Jun 19 00:00:00 EDT 2019
A Neural Circuit Encoding the Experience of Copulation in Female Drosophila.
Neuron
Shao L,Chung P,Wong A,Siwanowicz I,Kent CF,Long X,Heberlein U
A-11122 was used in Immunohistochemistry to investigate the effect of coital sensory inputs from those of male Drosophila ejaculate.
Wed Jun 05 00:00:00 EDT 2019
Recapitulation and Reversal of Schizophrenia-Related Phenotypes in Setd1a-Deficient Mice.
Neuron
Mukai J,Cannavò E,Crabtree GW,Sun Z,Diamantopoulou A,Thakur P,Chang CY,Cai Y,Lomvardas S,Takata A,Xu B,Gogos JA
A-11122 was used in Immunohistochemistry to investigate the bind mechanisms of SETD1A.
Wed Nov 06 00:00:00 EST 2019
Stromalin Constrains Memory Acquisition by Developmentally Limiting Synaptic Vesicle Pool Size.
Neuron
Phan A,Thomas CI,Chakraborty M,Berry JA,Kamasawa N,Davis RL
A-11122 was used in Immunohistochemistry to identify a new mechanism for memory suppression and to show that the size of the synaptic vesicle (SV) pool is controlled genetically and independently from other aspects of neuron structure and function through Stromalin in Drosophila.
Wed Jan 02 00:00:00 EST 2019
Punishment-Predictive Cues Guide Avoidance through Potentiation of Hypothalamus-to-Habenula Synapses.
Neuron
Trusel M,Nuno-Perez A,Lecca S,Harada H,Lalive AL,Congiu M,Takemoto K,Takahashi T,Ferraguti F,Mameli M
A-11122 was used in Immunocytochemistry-immunoflourescence to investigate foot-shock conditioning in mice to link the lateral habenula activation to avoidance learning.
Wed Apr 03 00:00:00 EDT 2019
Corticospinal circuit remodeling after central nervous system injury is dependent on neuronal activity.
The Journal of experimental medicine
Bradley PM,Denecke CK,Aljovic A,Schmalz A,Kerschensteiner M,Bareyre FM
A-11122 was used in Immunohistochemistry to show that NMDAR signaling and CREB-mediated transcription maintain nascent corticospinal tract (CST)-relay neuron contacts.
Mon Nov 04 00:00:00 EST 2019
Vector-mediated l-3,4-dihydroxyphenylalanine delivery reverses motor impairments in a primate model of Parkinson's disease.
Brain : a journal of neurology
Rosenblad C,Li Q,Pioli EY,Dovero S,Antunes AS,Agúndez L,Bardelli M,Linden RM,Henckaerts E,Björklund A,Bezard E,Björklund T
A-11122 was used in Immunohistochemistry to provide proof-of-principle for continuous vector-mediated l-DOPA synthesis as a novel therapeutic strategy for Parkinson's disease.
Thu Aug 01 00:00:00 EDT 2019
Induction of innate cytokine responses by respiratory mucosal challenge with R848 in zebrafish, mice, and humans.
The Journal of allergy and clinical immunology
Progatzky F,Jha A,Wane M,Thwaites RS,Makris S,Shattock RJ,Johansson C,Openshaw PJ,Bugeon L,Hansel TT,Dallman MJ
A-11122 was used in Immunohistochemistry-immunofluorescence to demonstrate that respiratory challenge with R848 might offer a novel mucosal model of antiviral immunity.
Mon Jul 01 00:00:00 EDT 2019
Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion.
The EMBO journal
Baba T,Toth DJ,Sengupta N,Kim YJ,Balla T
A-11122 was used in Immunocytochemistry to understand the molecular mechanism that triggers Rab7 activation.
Mon Apr 15 00:00:00 EDT 2019
Mutant Lef1 controls Gata6 in sebaceous gland development and cancer.
The EMBO journal
Oulès B,Rognoni E,Hoste E,Goss G,Fiehler R,Natsuga K,Quist S,Mentink R,Donati G,Watt FM
A-11122 was used in Immunohistochemistry to show that Gata6 is a β-catenin-independent transcriptional target of mutant Lef1.
Thu May 02 00:00:00 EDT 2019
Rap1b Promotes Notch-Signal-Mediated Hematopoietic Stem Cell Development by Enhancing Integrin-Mediated Cell Adhesion.
Developmental cell
Rho SS,Kobayashi I,Oguri-Nakamura E,Ando K,Fujiwara M,Kamimura N,Hirata H,Iida A,Iwai Y,Mochizuki N,Fukuhara S
A-11122 was used in Immunocytochemistry-immunoflourescence to identify the role of Rap1b in hematopoietic stem cell development in Zebrafish, by enhancing integrin-mediated cell adhesion within Notch signalling.
Mon Jun 03 00:00:00 EDT 2019
Sidekick Is a Key Component of Tricellular Adherens Junctions that Acts to Resolve Cell Rearrangements.
Developmental cell
Letizia A,He D,Astigarraga S,Colombelli J,Hatini V,Llimargas M,Treisman JE
A-11122 was used in Immunofluorescence on wholemounted tissues to suggest that Polychaetoid and Canoe link Sidekick to the actin cytoskeleton to enable tricellular adherens junctions to maintain or transmit cell bond tension during epithelial cell rearrangements.
Mon Aug 05 00:00:00 EDT 2019
Palmitoylation controls trafficking of the intracellular Ca2+ channel MCOLN3/TRPML3 to regulate autophagy.
Autophagy
Kim SW,Kim DH,Park KS,Kim MK,Park YM,Muallem S,So I,Kim HJ
A-11122 was used in Western Blotting to show that trafficking and channel function of MCOLN3/TRPML3 are regulated in the context of autophagy.
Fri Feb 01 00:00:00 EST 2019
Palmitoylation controls trafficking of the intracellular Ca2+ channel MCOLN3/TRPML3 to regulate autophagy.
Autophagy
Kim SW,Kim DH,Park KS,Kim MK,Park YM,Muallem S,So I,Kim HJ
A-11122 was used in Western Blotting to show that trafficking and channel function of MCOLN3/TRPML3 are regulated in the context of autophagy.
Fri Feb 01 00:00:00 EST 2019
SRSF3-Regulated RNA Alternative Splicing Promotes Glioblastoma Tumorigenicity by Affecting Multiple Cellular Processes.
Cancer research
Song X,Wan X,Huang T,Zeng C,Sastry N,Wu B,James CD,Horbinski C,Nakano I,Zhang W,Hu B,Cheng SY
A-11122 was used in Western Blotting to demonstrate that a major splicing factor was frequently upregulated in clinical glioma specimens and that elevated levels was associated with tumor progression and a poor prognosis for patients with glioma.
Tue Oct 15 00:00:00 EDT 2019
Targeting APLN/APLNR Improves Antiangiogenic Efficiency and Blunts Proinvasive Side Effects of VEGFA/VEGFR2 Blockade in Glioblastoma.
Cancer research
Mastrella G,Hou M,Li M,Stoecklein VM,Zdouc N,Volmar MNM,Miletic H,Reinhard S,Herold-Mende CC,Kleber S,Eisenhut K,Gargiulo G,Synowitz M,Vescovi AL,Harter PN,Penninger JM,Wagner E,Mittelbronn M,Bjerkvig R,Hambardzumyan D,Schüller U,Tonn JC,Radke J,Glass R,Kälin RE
A-11122 was used in Immunohistochemistry to show that apelin/APLNR signaling controls GBM angiogenesis and invasion and that both pathologic features are blunted by apelin-F13A.
Wed May 01 00:00:00 EDT 2019
Dual AAV-mediated gene therapy restores hearing in a DFNB9 mouse model.
Proceedings of the National Academy of Sciences of the United States of America
Akil O,Dyka F,Calvet C,Emptoz A,Lahlou G,Nouaille S,Boutet de Monvel J,Hardelin JP,Hauswirth WW,Avan P,Petit C,Safieddine S,Lustig LR
A-11122 was used in Immunohistochemistry-immunofluorescence to show adeno-associated virus based gene therapy can restore hearing in mice with DFNB9-mediated congenital deafness.
Tue Mar 05 00:00:00 EST 2019
Region-specific and activity-dependent regulation of SVZ neurogenesis and recovery after stroke.
Proceedings of the National Academy of Sciences of the United States of America
Liang H,Zhao H,Gleichman A,Machnicki M,Telang S,Tang S,Rshtouni M,Ruddell J,Carmichael ST
A-11122 was used in Immunohistochemistry to study the mechanisms regulating poststroke neurogenesis and its functional effect.
Tue Jul 02 00:00:00 EDT 2019
The AP2/ERF Transcription Factor TINY Modulates Brassinosteroid-Regulated Plant Growth and Drought Responses in Arabidopsis.
The Plant cell
Xie Z,Nolan T,Jiang H,Tang B,Zhang M,Li Z,Yin Y
A-11122 was used in Western Blotting to study the role of AP2/ERF family transcription factors in brassinosteroid-regulated growth and stress responses.
Thu Aug 01 00:00:00 EDT 2019
A Hybrid Approach Enabling Large-Scale Glycomic Analysis of Post-Golgi Vesicles Reveals a Transport Route for Polysaccharides.
The Plant cell
Wilkop T,Pattathil S,Ren G,Davis DJ,Bao W,Duan D,Peralta AG,Domozych DS,Hahn MG,Drakakaki G
A-11122 was used in Immunoprecipitation to provide evidence that SYP61 vesicles are involved in the transport and deposition of structural polysaccharides and glycoproteins.
Fri Mar 01 00:00:00 EST 2019
The MCTP-SNARE Complex Regulates Florigen Transport in Arabidopsis.
The Plant cell
Liu L,Li C,Teo ZWN,Zhang B,Yu H
A-11122 was used in Immunoprecipitation to show that the plasma membrane-resident syntaxin-like glutamine-soluble N-ethylmaleimide-sensitive factor protein attachment protein receptor, SYNTAXIN OF PLANTS121, interacts with QUIRKY, a member of the family of multiple C2 domain and transmembrane region proteins, to mediate FT transport in Arabidopsis.
Tue Oct 01 00:00:00 EDT 2019
Chloroplasts Modulate Elongation Responses to Canopy Shade by Retrograde Pathways Involving HY5 and Abscisic Acid.
The Plant cell
Ortiz-Alcaide M,Llamas E,Gomez-Cadenas A,Nagatani A,Martínez-García JF,Rodríguez-Concepción M
A-11122 was used in Chromatin immunoprecipitation to show that the connection among chloroplast status, light (shade) signaling, and developmental responses all contribute to achieve optimal photosynthetic performance under light-changing conditions.
Fri Feb 01 00:00:00 EST 2019
TBC1D24 regulates axonal outgrowth and membrane trafficking at the growth cone in rodent and human neurons.
Cell death and differentiation
Aprile D,Fruscione F,Baldassari S,Fadda M,Ferrante D,Falace A,Buhler E,Sartorelli J,Represa A,Baldelli P,Benfenati F,Zara F,Fassio A
A-11122 was used in Western Blot to investigate the impact of TBC1D24 knockdown during the first stages of neuronal differentiation when axonal specification and outgrowth take place.
Fri Nov 01 00:00:00 EDT 2019
Neural Evolution of Context-Dependent Fly Song.
Current biology : CB
Ding Y,Lillvis JL,Cande J,Berman GJ,Arthur BJ,Long X,Xu M,Dickson BJ,Stern DL
A-11122 was used in Immunohistochemistry-immunofluorescence to study the neural evolution of context-dependent fly song.
Mon Apr 01 00:00:00 EDT 2019
Polymodal Nociception in Drosophila Requires Alternative Splicing of TrpA1.
Current biology : CB
Gu P,Gong J,Shang Y,Wang F,Ruppell KT,Ma Z,Sheehan AE,Freeman MR,Xiang Y
A-11122 was used in Immunohistochemistry-immunofluorescence to develop a genetic approach to generate drosophila strains with knock-in TrpA1 isoforms.
Mon Dec 02 00:00:00 EST 2019
Modality-Specific Circuits for Skylight Orientation in the Fly Visual System.
Current biology : CB
Sancer G,Kind E,Plazaola-Sasieta H,Balke J,Pham T,Hasan A,Münch LO,Courgeon M,Mathejczyk TF,Wernet MF
A-11122 was used in Immunohistochemistry to investigate the cellular and synaptic architecture in medulla columns, where linearly polarized skylight is detected for guiding orientation responses in Drosophila melanogaster.
Mon Sep 09 00:00:00 EDT 2019
Oxytocin Signaling in the Central Amygdala Modulates Emotion Discrimination in Mice.
Current biology : CB
Ferretti V,Maltese F,Contarini G,Nigro M,Bonavia A,Huang H,Gigliucci V,Morelli G,Scheggia D,Managò F,Castellani G,Lefevre A,Cancedda L,Chini B,Grinevich V,Papaleo F
A-11122 was used in Immunohistochemistry to characterize the influence of endogenous OXT brain pathways on emotion discrimination in mice.
Mon Jun 17 00:00:00 EDT 2019
Gap Junction Coupling Shapes the Encoding of Light in the Developing Retina.
Current biology : CB
Caval-Holme F,Zhang Y,Feller MB
A-11122 was used in Immunohistochemistry-wholemount to show that both cell-autonomous intrinsic light responses and gap junction coupling among ipRGCs contribute to the proper encoding of light intensity in the developing retina.
Mon Dec 02 00:00:00 EST 2019
Chemokine signaling links cell-cycle progression and cilia formation for left-right symmetry breaking.
PLoS biology
Liu J,Zhu C,Ning G,Yang L,Cao Y,Huang S,Wang Q
A-11122 was used in Immunohistochemistry to uncover a role for Cxcr4 signaling in L-R patterning and provide fundamental insights into the molecular linkage between cell-cycle progression and ciliogenesis.
Thu Aug 01 00:00:00 EDT 2019
Changes in chromatin accessibility ensure robust cell cycle exit in terminally differentiated cells.
PLoS biology
Ma Y,McKay DJ,Buttitta L
A-11122 was used in Immunohistochemistry-immunofluorescence to provide evidence that developmentally programmed, temporal changes in chromatin accessibility at a small subset of critical cell cycle genes act to enforce cell cycle exit during terminal differentiation in the Drosophila melanogaster wing.
Sun Sep 01 00:00:00 EDT 2019
Chemosensory sensilla of the Drosophila wing express a candidate ionotropic pheromone receptor.
PLoS biology
He Z,Luo Y,Shang X,Sun JS,Carlson JR
A-11122 was used in Immunohistochemistry-immunofluorescence to provide a molecular description of the chemosensory sensilla of a greatly understudied taste organ and define a gene that regulates the sexual circuitry of the fly.
Wed May 01 00:00:00 EDT 2019
Glioblastoma cells vampirize WNT from neurons and trigger a JNK/MMP signaling loop that enhances glioblastoma progression and neurodegeneration.
PLoS biology
Portela M,Venkataramani V,Fahey-Lozano N,Seco E,Losada-Perez M,Winkler F,Casas-Tintó S
A-11122 was used in Immunohistochemistry to reveal a molecular mechanism for TM production, infiltration, and maintenance that can explain both neuron-dependent tumor progression and also the neural decay associated with GB.
Sun Dec 01 00:00:00 EST 2019
L-Serine dietary supplementation is associated with clinical improvement of loss-of-function GRIN2B-related pediatric encephalopathy.
Science signaling
Soto D,Olivella M,Grau C,Armstrong J,Alcon C,Gasull X,Santos-Gómez A,Locubiche S,Gómez de Salazar M,García-Díaz R,Gratacòs-Batlle E,Ramos-Vicente D,Chu-Van E,Colsch B,Fernández-Dueñas V,Ciruela F,Bayés À,Sindreu C,López-Sala A,García-Cazorla À,Altafaj X
A-11122 was used in Immunocytochemistry-immunoflourescence to suggest that l-serine supplementation might ameliorate GRIN2B-related severe encephalopathy and other neurological conditions caused by glutamatergic signaling deficiency.
Tue Jun 18 00:00:00 EDT 2019
Trehalose-6-phosphate signaling regulates thermoresponsive hypocotyl growth in Arabidopsis thaliana.
EMBO reports
Hwang G,Kim S,Cho JY,Paik I,Kim JI,Oh E
A-11122 was used in Immunocytochemistry-immunoflourescence to study the regulation of thermoresponsive growth by trehalose-6-phosphate in Arabidopsis thaliana.
Fri Oct 04 00:00:00 EDT 2019
Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata.
EMBO reports
Brault ML,Petit JD,Immel F,Nicolas WJ,Glavier M,Brocard L,Gaston A,Fouché M,Hawkins TJ,Crowet JM,Grison MS,Germain V,Rocher M,Kraner M,Alva V,Claverol S,Paterlini A,Helariutta Y,Deleu M,Lins L,Tilsner J,Bayer EM
A-11122 was used in Immunohistochemistry on frozen tissues to study the molecular mechanism and function of membrane tethering with plasmodesomata.
Thu Aug 01 00:00:00 EDT 2019
A novel cross-talk between CXCR4 and PI4KIIIα in prostate cancer cells.
Oncogene
Sbrissa D,Semaan L,Govindarajan B,Li Y,Caruthers NJ,Stemmer PM,Cher ML,Sethi S,Vaishampayan U,Shisheva A,Chinni SR
A-11122 was used in Western Blotting to investigate the relationship between the activity of CXCR4 and PI4KIIIα in prostate cancer cells, and identify a novel cross-talk between them.
Tue Jan 01 00:00:00 EST 2019
FIGNL1 associates with KIF1Bβ and BICD1 to restrict dynein transport velocity during axon navigation.
The Journal of cell biology
Atkins M,Gasmi L,Bercier V,Revenu C,Del Bene F,Hazan J,Fassier C
A-11122 was used in Immunocytochemistry-immunoflourescence to identify Fignl1 as a key dynein regulator required for motor circuit wiring.
Mon Oct 07 00:00:00 EDT 2019
FIGNL1 associates with KIF1Bβ and BICD1 to restrict dynein transport velocity during axon navigation.
The Journal of cell biology
Atkins M,Gasmi L,Bercier V,Revenu C,Del Bene F,Hazan J,Fassier C
A-11122 was used in Immunocytochemistry-immunoflourescence to identify Fignl1 as a key dynein regulator required for motor circuit wiring.
Mon Oct 07 00:00:00 EDT 2019
FIGNL1 associates with KIF1Bβ and BICD1 to restrict dynein transport velocity during axon navigation.
The Journal of cell biology
Atkins M,Gasmi L,Bercier V,Revenu C,Del Bene F,Hazan J,Fassier C
A-11122 was used in Immunocytochemistry-immunoflourescence to identify Fignl1 as a key dynein regulator required for motor circuit wiring.
Mon Oct 07 00:00:00 EDT 2019
The molecular architecture of the meiotic spindle is remodeled during metaphase arrest in oocytes.
The Journal of cell biology
Costa MFA,Ohkura H
A-11122 was used in Immunohistochemistry-immunofluorescence to provide novel insight into how oocytes maintain a bipolar spindle during metaphase arrest.
Mon Sep 02 00:00:00 EDT 2019
Dynamic nanoscale morphology of the ER surveyed by STED microscopy.
The Journal of cell biology
Schroeder LK,Barentine AES,Merta H,Schweighofer S,Zhang Y,Baddeley D,Bewersdorf J,Bahmanyar S
A-11122 was used in Immunocytochemistry-immunoflourescence to suggest the ER contains a continuum of membrane structures that includes dynamic nanoholes in sheets as well as clustered tubules.
Mon Jan 07 00:00:00 EST 2019
Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15.
The Journal of cell biology
Malaby HLH,Dumas ME,Ohi R,Stumpff J
A-11122 was used in Immunocytochemistry-immunoflourescence to demonstrate that kinesin-binding protein (KBP) reduces the activity of KIF18A and KIF15 during metaphase.
Mon Apr 01 00:00:00 EDT 2019
ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network.
The Journal of cell biology
Ishida M,Bonifacino JS
A-11122 was used in Western Blotting to demonstrate that ARFRP1 is a master regulator of retrograde-carrier tethering to the TGN. The coordinated recruitment of distinct tethering factors by a bifurcated GTPase cascade may be paradigmatic of other vesicular fusion events within the cell.
Mon Nov 04 00:00:00 EST 2019
Glucose-Regulated HLP1 Acts as a Key Molecule in Governing Thermomemory.
Plant physiology
Sharma M,Banday ZZ,Shukla BN,Laxmi A
A-11122 was used in Chromatin immunoprecipitation to reveal the novel function of Glc-regulated HLP1 in mediating thermotolerance/thermomemory response.
Sat Jun 01 00:00:00 EDT 2019
A Cut/cohesin axis alters the chromatin landscape to facilitate neuroblast death.
Development (Cambridge, England)
Arya R,Gyonjyan S,Harding K,Sarkissian T,Li Y,Zhou L,White K
A-11122 was used in Immunohistochemistry to show that the Cut DNA binding protein is required for neuroblast death, regulating reaper and grim downstream of the shared enhancer and of abdominal A expression.
Wed May 01 00:00:00 EDT 2019
PRDM1 controls the sequential activation of neural, neural crest and sensory progenitor determinants.
Development (Cambridge, England)
Prajapati RS,Hintze M,Streit A
A-11122 was used in Immunocytochemistry to identify the role of the transcription factor PRDM1 in ectodermal cell fate allocation during early embryogenesis.
Mon Dec 16 00:00:00 EST 2019
Smooth muscle differentiation shapes domain branches during mouse lung development.
Development (Cambridge, England)
Goodwin K,Mao S,Guyomar T,Miller E,Radisky DC,Košmrlj A,Nelson CM
A-11122 was used in Immunohistochemistry-immunofluorescence to shed light on the physical mechanisms of branching morphogenesis in the mouse lung.
Mon Nov 25 00:00:00 EST 2019
Wound-induced polyploidization is driven by Myc and supports tissue repair in the presence of DNA damage.
Development (Cambridge, England)
Grendler J,Lowgren S,Mills M,Losick VP
A-11122 was used in Immunohistochemistry to find that wound-induced polyploidization enables tissue repair when cell division is not a viable option in adult Drosophila epithelium.
Fri Aug 02 00:00:00 EDT 2019
Lar maintains the homeostasis of the hematopoietic organ in Drosophila by regulating insulin signaling in the niche.
Development (Cambridge, England)
Kaur H,Sharma SK,Mandal S,Mandal L
A-11122 was used in Immunoprecipitation to study if Lar physically associates with InR and serves as a negative regulator for insulin signaling in Drosophila larval hematopoietic niche.
Mon Dec 23 00:00:00 EST 2019
The Paf1 complex and P-TEFb have reciprocal and antagonist roles in maintaining multipotent neural crest progenitors.
Development (Cambridge, England)
Jurynec MJ,Bai X,Bisgrove BW,Jackson H,Nechiporuk A,Palu RAS,Grunwald HA,Su YC,Hoshijima K,Yost HJ,Zon LI,Grunwald DJ
A-11122 was used in Immunohistochemistry to analyse alyron mutant embryos carrying a null mutation in paf1.
Mon Dec 16 00:00:00 EST 2019
H3K27me3-mediated silencing of structural genes is required for zebrafish heart regeneration.
Development (Cambridge, England)
Ben-Yair R,Butty VL,Busby M,Qiu Y,Levine SS,Goren A,Boyer LA,Burns CG,Burns CE
A-11122 was used in Immunohistochemistry-immunofluorescence to identify H3K27me3-mediated silencing of structural genes as requisite for zebrafish heart regeneration and suggests that repression of similar structural components in the border zone of an infarcted human heart might improve its regenerative capacity.
Wed Oct 09 00:00:00 EDT 2019
E2FA and E2FB transcription factors coordinate cell proliferation with seed maturation.
Development (Cambridge, England)
Leviczky T,Molnár E,Papdi C,Őszi E,Horváth GV,Vizler C,Nagy V,Pauk J,Bögre L,Magyar Z
A-11122 was used in Chromatin immunoprecipitation to reveal a repressor function of the activator E2Fs to restrict the seed maturation programme until the cell proliferation phase is completed.
Tue Nov 26 00:00:00 EST 2019
Topography of Reward and Aversion Encoding in the Mesolimbic Dopaminergic System.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Yuan L,Dou YN,Sun YG
A-11122 was used in Immunohistochemistry to extend our knowledge about how the mesolimbic DA system process motivational information at the circuitry level.
Wed Aug 14 00:00:00 EDT 2019
AGRP Neurons Project to the Medial Preoptic Area and Modulate Maternal Nest-Building.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Li XY,Han Y,Zhang W,Wang SR,Wei YC,Li SS,Lin JK,Yan JJ,Chen AX,Zhang X,Zhao ZD,Shen WL,Xu XH
A-11122 was used in Immunohistochemistry to discover that activation of AGRP→mPOA projections in female mice inhibits maternal nest-building but pup retrieval behaviour is mostly similar is mostly unchanged.
Wed Jan 16 00:00:00 EST 2019
A Subpopulation of Amygdala Neurons Mediates the Affective Component of Itch.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Sanders KM,Sakai K,Henry TD,Hashimoto T,Akiyama T
A-11122 was used in Immunohistochemistry to show activation of histamine-responsive amygdala neurons enhanced scratching indicating an importance in the itch-response.
Wed Apr 24 00:00:00 EDT 2019
The Inhibition of RasGRF2, But Not RasGRF1, Alters Cocaine Reward in Mice.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Bernardi RE,Olevska A,Morella I,Fasano S,Santos E,Brambilla R,Spanagel R
A-11122 was used in Immunohistochemistry to study Ras-ERK signalling and its upstream regulators in drug taking behaviour.
Wed Aug 07 00:00:00 EDT 2019
Chemogenetic Inhibition Reveals That Processing Relative But Not Absolute Threat Requires Basal Amygdala.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Campese VD,Kim IT,Hou M,Gupta S,Draus C,Kurpas B,Burke K,LeDoux JE
A-11122 was used in Immunohistochemistry-immunofluorescence to study the role of basal amygdala in discriminating between different aversive stimuli and the appropriate response to them.
Wed Oct 23 00:00:00 EDT 2019
Functional Coordination of the Chromatin-Remodeling Factor AtINO80 and the Histone Chaperones NRP1/2 in Inflorescence Meristem and Root Apical Meristem.
Frontiers in plant science
Kang H,Ma J,Wu D,Shen WH,Zhu Y
A-11122 was used in Chromatin immunoprecipitation to provide novel insight into the functional coordination of the two epigenetic factors AtINO80 and NRP1/2 in apical meristems during plant growth and development.
Tue Sep 29 00:00:00 EDT 2020
Lack of Myosin X Enhances Osteoclastogenesis and Increases Cell Surface Unc5b in Osteoclast-Lineage Cells.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Wang B,Pan JX,Yu H,Xiong L,Zhao K,Xiong S,Guo JP,Lin S,Sun D,Zhao L,Guo H,Mei L,Xiong WC
A-11122 was used in Immunocytochemistry-Immunofluorescence to study the effects of Myo10 loss of function on osteoporotic regulation in mice models.
Wed May 01 00:00:00 EDT 2019
High-Affinity DARPin Allows Targeting of MeV to Glioblastoma Multiforme in Combination with Protease Targeting without Loss of Potency.
Molecular therapy oncolytics
Hanauer JRH,Koch V,Lauer UM,Mühlebach MD
A-11122 was used in Western Blotting to generate a receptor-targeted MeV by using receptor-blind viral attachment protein genetically fused to designed ankyrin repeat protein (DARPin) binding domains specific for the epidermal growth factor receptor (EGFR).
Fri Dec 20 00:00:00 EST 2019
Peripheral Delta Opioid Receptors Mediate Formoterol Anti-allodynic Effect in a Mouse Model of Neuropathic Pain.
Frontiers in molecular neuroscience
Ceredig RA,Pierre F,Doridot S,Alduntzin U,Hener P,Salvat E,Yalcin I,Gaveriaux-Ruff C,Barrot M,Massotte D
A-11122 was used in Immunohistochemistry to confirm that changes in the mechanical threshold are associated with changes in peripheral DOP profile and to highlight the common impact on DOP receptors between serotonin noradrenaline reuptake inhibitors such as duloxetine and the β2 mimetic formoterol.
Mon Sep 28 00:00:00 EDT 2020
NCS-1 Deficiency Is Associated With Obesity and Diabetes Type 2 in Mice.
Frontiers in molecular neuroscience
Ratai O,Hermainski J,Ravichandran K,Pongs O
A-11122 was used in Immunohistochemistry to show that NCS-1 plays an important role in adipocyte function and that NCS-1 deficiency gives rise to obesity and diabetes type 2 in adult mice.
Tue Sep 29 00:00:00 EDT 2020
ULK2 is essential for degradation of ubiquitinated protein aggregates and homeostasis in skeletal muscle.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Fuqua JD,Mere CP,Kronemberger A,Blomme J,Bae D,Turner KD,Harris MP,Scudese E,Edwards M,Ebert SM,de Sousa LGO,Bodine SC,Yang L,Adams CM,Lira VA
A-11122 was used in Immunohistochemistry to reveal unc-51 like autophagy activating kinase (ULK)2 to be an essential protein for degradation of ubiquitinated proteins and homeostasis in skeletal muscle.
Fri Nov 01 00:00:00 EDT 2019
Exploitation of Synthetic mRNA To Drive Immune Effector Cell Recruitment and Functional Reprogramming In Vivo.
Journal of immunology (Baltimore, Md. : 1950)
Xu Y,Huang L,Kirschman JL,Vanover DA,Tiwari PM,Santangelo PJ,Shen X,Russell DG
A-11122 was used in Immunohistochemistry to demonstrate how IVT strategies can be combined to recruit and reprogram immune effector cells that have the capacity to fulfill complex biological tasks in vivo.
Tue Jan 15 00:00:00 EST 2019
Genome-wide identification of alternative splicing events that regulate protein transport across the secretory pathway.
Journal of cell science
Neumann A,Schindler M,Olofsson D,Wilhelmi I,Schürmann A,Heyd F
A-11122 was used in Blocking experiments to identify over 200 alternative splicing events as secretion regulators.
Thu Apr 25 00:00:00 EDT 2019
An autism-associated mutation in GluN2B prevents NMDA receptor trafficking and interferes with dendrite growth.
Journal of cell science
Sceniak MP,Fedder KN,Wang Q,Droubi S,Babcock K,Patwardhan S,Wright-Zornes J,Pham L,Sabo SL
A-11122 was used in Immunocytochemistry to investigate cellular phenotypes induced by a human mutation that is predicted to truncate GluN2B within the extracellular loop.
Fri Oct 18 00:00:00 EDT 2019
Prx1-Expressing Progenitor Primary Cilia Mediate Bone Formation in response to Mechanical Loading in Mice.
Stem cells international
Moore ER,Chen JC,Jacobs CR
A-11122 was used in Immunohistochemistry to show that Prx1-expressing cells become embedded osteocytes in response to physical loading and this mechanism requires the primary cilium.
Thu Oct 01 00:00:00 EDT 2020
Loss-of-function mutations in KIF14 cause severe microcephaly and kidney development defects in humans and zebrafish.
Human molecular genetics
Reilly ML,Stokman MF,Magry V,Jeanpierre C,Alves M,Paydar M,Hellinga J,Delous M,Pouly D,Failler M,Martinovic J,Loeuillet L,Leroy B,Tantau J,Roume J,Gregory-Evans CY,Shan X,Filges I,Allingham JS,Kwok BH,Saunier S,Giles RH,Benmerah A
A-11122 was used in Western Blot to show severe syndrome associating microcephaly and renal hypodysplasia can be triggered by mutations in mitotic kinesin KIF14.
Fri Mar 01 00:00:00 EST 2019
Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.
Human molecular genetics
Dupont MA,Humbert C,Huber C,Siour Q,Guerrera IC,Jung V,Christensen A,Pouliet A,Garfa-Traoré M,Nitschké P,Injeyan M,Millar K,Chitayat D,Shannon P,Girisha KM,Shukla A,Mechler C,Lorentzen E,Benmerah A,Cormier-Daire V,Jeanpierre C,Saunier S,Delous M
A-11122 was used in Immunocytochemistry-immunoflourescence to investigate if short-rib thoracic dysplasia-associated missense mutation impairs intraflagellar transport-B (IFT) complex assembly and IFT-B2 ciliary localization, resulting in decreased cilia length.
Thu Aug 15 00:00:00 EDT 2019
A neurodevelopmental disorder caused by mutations in the VPS51 subunit of the GARP and EARP complexes.
Human molecular genetics
Gershlick DC,Ishida M,Jones JR,Bellomo A,Bonifacino JS,Everman DB
A-11122 was used in Western Blotting to study role of GARP/EARP complexes in cellular and organismal physiology and how this relates to neurodevelopmental disorders.
Wed May 01 00:00:00 EDT 2019
Vascular Endothelial Growth Factor Delivery to Placental Basal Plate Promotes Uterine Artery Remodeling in the Primate.
Endocrinology
Babischkin JS,Aberdeen GW,Lindner JR,Bonagura TW,Pepe GJ,Albrecht ED
A-11122 was used in Immunocytochemistry-immunoflourescence to establish the role of extravillous trophoblast vascular endothelial growth factor in elevating estradiol levels and regulating uterine artery remodeling, promoting placental blood flow in early baboon pregnancy.
Sat Jun 01 00:00:00 EDT 2019
GSK3β Modulates Timing-Dependent Long-Term Depression Through Direct Phosphorylation of Kv4.2 Channels.
Cerebral cortex (New York, N.Y. : 1991)
Aceto G,Re A,Mattera A,Leone L,Colussi C,Rinaudo M,Scala F,Gironi K,Barbati SA,Fusco S,Green T,Laezza F,D'Ascenzo M,Grassi C
A-11122 was used in Immunoprecipitation to identify the functional interaction between GSK3β and Kv4.2 channel as a novel mechanism for tLTD modulation.
Wed May 01 00:00:00 EDT 2019
Split-QF System for Fine-Tuned Transgene Expression in Drosophila.
Genetics
Riabinina O,Vernon SW,Dickson BJ,Baines RA
A-11122 was used in Immunohistochemistry-immunofluorescence to report the development and demonstrate the applications of a split-QF system that drives strong expression in Drosophila, is repressible by QS and is inducible by a small nontoxic molecule.
Wed May 01 00:00:00 EDT 2019
Renal Na+ excretion consequent to pharmacogenetic activation of Gq-DREADD in principal cells.
American journal of physiology. Renal physiology
Mironova E,Suliman F,Stockand JD
A-11122 was used in Immunohistochemistry to demonstrate that selective activation of the P2Y2 receptor and Gq signaling in principal cells is sufficient to promote renal salt excretion.
Mon Apr 01 00:00:00 EDT 2019
Subcellular Localization and Activity of the Mitogen-Activated Protein Kinase Kinase 7 (MKK7) γ Isoform are Regulated through Binding to the Phosphatase Calcineurin.
Molecular pharmacology
Gibson ES,Woolfrey KM,Li H,Hogan PG,Nemenoff RA,Heasley LE,Dell'Acqua ML
A-11122 was used in Immunoprecipitation to identify the docking of CaN to the γ isoform of MKK7, a component of the c-Jun N-terminal kinase (JNK) pathway, to regulate it's subcellular localisation and activity.
Tue Jan 01 00:00:00 EST 2019
Subcellular Localization and Activity of the Mitogen-Activated Protein Kinase Kinase 7 (MKK7) γ Isoform are Regulated through Binding to the Phosphatase Calcineurin.
Molecular pharmacology
Gibson ES,Woolfrey KM,Li H,Hogan PG,Nemenoff RA,Heasley LE,Dell'Acqua ML
A-11122 was used in Immunoprecipitation to identify the docking of CaN to the γ isoform of MKK7, a component of the c-Jun N-terminal kinase (JNK) pathway, to regulate it's subcellular localisation and activity.
Tue Jan 01 00:00:00 EST 2019
Ano1 mediates pressure-sensitive contraction frequency changes in mouse lymphatic collecting vessels.
The Journal of general physiology
Zawieja SD,Castorena JA,Gui P,Li M,Bulley SA,Jaggar JH,Rock JR,Davis MJ
A-11122 was used in Immunocytochemistry-immunoflourescence to show that the canonical CaCC Anoctamin 1 (Ano1, TMEM16a) plays an important role in lymphatic smooth muscle pacemaking.
Mon Apr 01 00:00:00 EDT 2019
Ventrolateral periaqueductal gray mediates rapid eye movement sleep regulation by melanin-concentrating hormone neurons.
Neuroscience
Kroeger D,Bandaru SS,Madara JC,Vetrivelan R
A-11122 was used in Immunohistochemistry to show rapid eye movement sleep may be modulated through an essential relay provided by the ventrolateral periaqueductal gray and the lateral pontine tegmentum (vlPAG/LPT).
Wed May 15 00:00:00 EDT 2019
Neuronal Activity in Non-LNv Clock Cells Is Required to Produce Free-Running Rest:Activity Rhythms in Drosophila.
Journal of biological rhythms
Bulthuis N,Spontak KR,Kleeman B,Cavanaugh DJ
A-11122 was used in Immunohistochemistry to report the discovery of Mortaparib as a dual inhibitor of mortalin and PARP1 and identify as a potential anticancer drug due to its tumor suppressor activity.
Sat Jun 01 00:00:00 EDT 2019
Replication of HIV-1 envelope protein cytoplasmic domain variants in permissive and restrictive cells.
Virology
Staubus AO,Alfadhli A,Barklis RL,Barklis E
A-11122 was used in Immunocytochemistry-immunoflourescence to study how cytoplasmic tails (CT) elements regulate HIV-1 envelope (Env) protein function.
Sun Dec 01 00:00:00 EST 2019
KRAS and BRAF mutations induce anoikis resistance and characteristic 3D phenotypes in Caco‑2 cells.
Molecular medicine reports
Patankar M,Eskelinen S,Tuomisto A,Mäkinen MJ,Karttunen TJ
A-11122 was used in Western Blotting to demonstrate that KRAS and BRAF mutations induce anoikis resistance in colon cancer cells.
Fri Nov 01 00:00:00 EDT 2019
Cellular Dynamics during Spinal Cord Regeneration in Larval Zebrafish.
Developmental neuroscience
Anguita-Salinas C,Sánchez M,Morales RA,Ceci ML,Rojas-Benítez D,Allende ML
A-11122 was used in Immunohistochemistry to show that severed spinal axons exhibited varying regenerative capabilities and plasticity depending on their original dorsoventral position in the spinal cord.
Mon Mar 30 00:00:00 EDT 2020
AAV-Mediated Neurotrophin Gene Therapy Promotes Improved Survival of Cochlear Spiral Ganglion Neurons in Neonatally Deafened Cats: Comparison of AAV2-hBDNF and AAV5-hGDNF.
Journal of the Association for Research in Otolaryngology : JARO
Leake PA,Rebscher SJ,Dore' C,Akil O
A-11122 was used in Immunocytochemistry-immunoflourescence to investigate the potential of adeno-associated viral vectors to elicit targeted neurotrophic factor expression in the cochlea and promote improved spiral ganglion and radial nerve fiber survival.
Thu Aug 01 00:00:00 EDT 2019
The Ciona myogenic regulatory factor functions as a typical MRF but possesses a novel N-terminus that is essential for activity.
Developmental biology
Ratcliffe LE,Asiedu EK,Pickett CJ,Warburton MA,Izzi SA,Meedel TH
A-11122 was used in Immunohistochemistry-immunofluorescence to indicate that ascidians have previously unrecognized and potentially novel requirements for MRF-directed myogenesis.
Mon Apr 15 00:00:00 EDT 2019
Epigenetic factors Dnmt1 and Uhrf1 coordinate intestinal development.
Developmental biology
Ganz J,Melancon E,Wilson C,Amores A,Batzel P,Strader M,Braasch I,Diba P,Kuhlman JA,Postlethwait JH,Eisen JS
A-11122 was used in Immunohistochemistry-immunofluorescence to find that functional loss of a DNA methylation machinery component, ubiquitin-like protein containing PHD and RING finger domain 1, leads to reduced numbers of ectoderm-derived enteric neurons and severe disruption of mesoderm-derived intestinal smooth muscle.
Fri Nov 15 00:00:00 EST 2019
Decoupling developmental apoptosis and neuroblast proliferation in Drosophila.
Developmental biology
Harding K,White K
A-11122 was used in Immunohistochemistry to demonstrate that upstream control of neuroblast proliferation and apoptosis represent independent mechanisms of regulating stem cell fate, and that execution of apoptosis occurs in a cell cycle-independent manner.
Sun Dec 01 00:00:00 EST 2019
Structure-function analysis of β-arrestin Kurtz reveals a critical role of receptor interactions in downregulation of GPCR signaling in vivo.
Developmental biology
Chai F,Xu W,Musoke T,Tarabelsi G,Assaad S,Freedman J,Peterson R,Piotrowska K,Byrnes J,Rogers S,Veraksa A
A-11122 was used in Immunocytochemistry-immunoflourescence to analyse the in vivo structure and function of Kurtz within the drosophila.
Fri Nov 15 00:00:00 EST 2019
Avian Adeno-Associated Viral Transduction of the Postembryonic Chicken Retina.
Translational vision science & technology
Waldner DM,Visser F,Fischer AJ,Bech-Hansen NT,Stell WK
A-11122 was used in Immunohistochemistry to adapt the adeno-associated viral (AAV) vector method, used widely in mammalian studies, for use in investigations of the chicken retina.
Mon Jul 01 00:00:00 EDT 2019
Tmem2 restricts atrioventricular canal differentiation by regulating degradation of hyaluronic acid.
Developmental dynamics : an official publication of the American Association of Anatomists
Hernandez L,Ryckebüsch L,Wang C,Ling R,Yelon D
A-11122 was used in Western Blot to support a model in which HA degradation, regulated by Tmem2, limits the distribution of Wnt signaling and thereby confines the differentiation of the AVC.
Sun Dec 01 00:00:00 EST 2019
Zebrafish model of KRAS-initiated pancreatic endocrine tumor.
Animal cells and systems
Oh S,Park JT
A-11122 was used in Immunohistochemistry to establish a zebrafish model as a suitable experimental system with which to assess the effectiveness of strategies for targeting pancreatic endocrine tumours.
Sat Jun 01 00:00:00 EDT 2019
Optogenetic inhibition of the medial prefrontal cortex reduces methamphetamine-primed reinstatement in male and female rats.
Behavioural pharmacology
Cordie R,McFadden LM
A-11122 was used in Immunohistochemistry to investigate if the prelimbic portion of the mPFC plays a role in methamphetamine-seeking behaviour using rat models.
Sun Sep 01 00:00:00 EDT 2019
Englerin A-sensing charged residues for transient receptor potential canonical 5 channel activation.
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology
Jeong S,Ko J,Kim M,Park KC,Park EYJ,Kim J,Baik Y,Wie J,Cho AE,Jeon JH,So I
A-11122 was used in Western Blotting to suggest that although other pore residues are not critical, they could partly contribute to the EA-induced channel activation.
Wed May 01 00:00:00 EDT 2019
Application of CRISPR/Cas9 editing and digital droplet PCR in human iPSCs to generate novel knock-in reporter lines to visualize dopaminergic neurons.
Stem cell research
Überbacher C,Obergasteiger J,Volta M,Venezia S,Müller S,Pesce I,Pizzi S,Lamonaca G,Picard A,Cattelan G,Malpeli G,Zoli M,Beccano-Kelly D,Flynn R,Wade-Martins R,Pramstaller PP,Hicks AA,Cowley SA,Corti C
A-11122 was used in Immunocytochemistry-immunoflourescence to generate a model to investigate the system for dopaminergic neurones in Parkinson's.
Sun Dec 01 00:00:00 EST 2019
Mi-2/NuRD complex protects stem cell progeny from mitogenic Notch signaling.
eLife
Zacharioudaki E,Falo Sanjuan J,Bray S
A-11122 was used in Immunoprecipitation to show that depletion of the Mi-2/NuRD ATP remodelling complex dramatically enhances Notch-induced hyperplasia in neural stem cells of Drosophila.
Tue Jan 29 00:00:00 EST 2019
Mi-2/NuRD complex protects stem cell progeny from mitogenic Notch signaling.
eLife
Zacharioudaki E,Falo Sanjuan J,Bray S
A-11122 was used in Immunoprecipitation to show that depletion of the Mi-2/NuRD ATP remodelling complex dramatically enhances Notch-induced hyperplasia in neural stem cells of Drosophila.
Tue Jan 29 00:00:00 EST 2019
Arabidopsis S2Lb links AtCOMPASS-like and SDG2 activity in H3K4me3 independently from histone H2B monoubiquitination.
Genome biology
Fiorucci AS,Bourbousse C,Concia L,Rougée M,Deton-Cabanillas AF,Zabulon G,Layat E,Latrasse D,Kim SK,Chaumont N,Lombard B,Stroebel D,Lemoine S,Mohammad A,Blugeon C,Loew D,Bailly C,Bowler C,Benhamed M,Barneche F
A-11122 was used in Immunoprecipitation to study the evolutionarily conserved COMPASS-like complex in plant systems.
Tue May 21 00:00:00 EDT 2019
Cigarette smoke extracts and cadmium induce COX-2 expression through γ-secretase-mediated p38 MAPK activation in C6 astroglia cells.
PloS one
Lim HJ,Park JH,Jo C,Yoon K,Koh YH
A-11122 was used in Western Blotting to investigate the signaling cascade activated by cigarette smoke extracts (CSE) and cadmium (Cd) resulting in the COX-2 induction in C6 rat astroglia cells.
Thu Nov 21 00:00:00 EST 2019
Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification.
eLife
Nam H,Jeon S,An H,Yoo J,Lee HJ,Lee SK,Lee S
A-11122 was used in Immunohistochemistry to reveal that Shh, secreted from MNs, plays a crucial role in generating MN diversity via a regulatory axis of Shh-AKT-ARHGAP36 in the developing mouse spinal cord.
Mon Jul 15 00:00:00 EDT 2019
Targeted sequencing of DCSTAMP in familial Paget's disease of bone.
Bone reports
Sultana MA,Pavlos NJ,Ward L,Walsh JP,Rea SL
A-11122 was used in Western Blotting to conclude that genetic variation in DCSTAMP is not a significant predisposing factor in our specific cohort of PDB patients and the p.L397F variant is unlikely to be a contributing factor in PDB pathogenesis.
Sat Jun 01 00:00:00 EDT 2019
Retrograde Ret signaling controls sensory pioneer axon outgrowth.
eLife
Tuttle A,Drerup CM,Marra M,McGraw H,Nechiporuk AV
A-11122 was used in Immunohistochemistry to show a specific role for Ret51 in pioneer axon growth, and suggest a critical role for long-range retrograde Ret signaling in regulating growth cone dynamics through downstream transcriptional changes.
Mon Sep 02 00:00:00 EDT 2019
Optimized CRISPR/Cas9-mediated in vivo genome engineering applicable to monitoring dynamics of endogenous proteins in the mouse neural tissues.
Scientific reports
Matsuda T,Oinuma I
A-11122 was used in Immunohistochemistry to disclose a powerful and widely applicable approach for visualization and manipulation of endogenous proteins in neural tissues.
Mon Aug 05 00:00:00 EDT 2019
TFPa/HADHA is required for fatty acid beta-oxidation and cardiolipin re-modeling in human cardiomyocytes.
Nature communications
Miklas JW,Clark E,Levy S,Detraux D,Leonard A,Beussman K,Showalter MR,Smith AT,Hofsteen P,Yang X,Macadangdang J,Manninen T,Raftery D,Madan A,Suomalainen A,Kim DH,Murry CE,Fiehn O,Sniadecki NJ,Wang Y,Ruohola-Baker H
A-11122 was used in Immunocytochemistry-immunoflourescence to reveal that HADHA is requited for fatty acid beta-oxidation and cardiolipin remodelling, essential for functional mitochondria in human cardiomyocytes.
Fri Oct 11 00:00:00 EDT 2019
TFPa/HADHA is required for fatty acid beta-oxidation and cardiolipin re-modeling in human cardiomyocytes.
Nature communications
Miklas JW,Clark E,Levy S,Detraux D,Leonard A,Beussman K,Showalter MR,Smith AT,Hofsteen P,Yang X,Macadangdang J,Manninen T,Raftery D,Madan A,Suomalainen A,Kim DH,Murry CE,Fiehn O,Sniadecki NJ,Wang Y,Ruohola-Baker H
A-11122 was used in Immunocytochemistry-immunoflourescence to reveal that HADHA is requited for fatty acid beta-oxidation and cardiolipin remodelling, essential for functional mitochondria in human cardiomyocytes.
Fri Oct 11 00:00:00 EDT 2019
Methamphetamine regulation of activity and topology of ventral midbrain networks.
PloS one
Miller DR,Lebowitz JJ,Guenther DT,Refowich AJ,Hansen C,Maurer AP,Khoshbouei H
A-11122 was used in Immunocytochemistry to establish primary ventral midbrain networks culture as a viable model system that reveals specific changes in network activity, connectivity, and topology modulation by methamphetamine.
Mon Mar 23 00:00:00 EDT 2020
Enterovirus pathogenesis requires the host methyltransferase SETD3.
Nature microbiology
Diep J,Ooi YS,Wilkinson AW,Peters CE,Foy E,Johnson JR,Zengel J,Ding S,Weng KF,Laufman O,Jang G,Xu J,Young T,Verschueren E,Kobluk KJ,Elias JE,Sarnow P,Greenberg HB,Hüttenhain R,Nagamine CM,Andino R,Krogan NJ,Gozani O,Carette JE
A-11122 was used in Immunocytochemistry-immunoflourescence to study how Enteroviruses (EVs) co-opt cellular functions to promote replication and spread.
Sun Dec 01 00:00:00 EST 2019
Periosteum-derived mesenchymal progenitor cells in engineered implants promote fracture healing in a critical-size defect rat model.
Journal of tissue engineering and regenerative medicine
González-Gil AB,Lamo-Espinosa JM,Muiños-López E,Ripalda-Cemboráin P,Abizanda G,Valdés-Fernández J,López-Martínez T,Flandes-Iparraguirre M,Andreu I,Elizalde MR,Stuckensen K,Groll J,De-Juan-Pardo EM,Prósper F,Granero-Moltó F
A-11122 was used in Immunohistochemistry to compare the therapeutic potential of different sources of mesenchymal stem cells in combination with biomaterials in a bone nonunion model.
Wed May 01 00:00:00 EDT 2019
Gamma Entrainment Binds Higher-Order Brain Regions and Offers Neuroprotection.
Neuron
Adaikkan C,Middleton SJ,Marco A,Pao PC,Mathys H,Kim DN,Gao F,Young JZ,Suk HJ,Boyden ES,McHugh TJ,Tsai LH
A-11122 was used in Immunohistochemistry to study the role of gamma entertainment using sensory stimuli can affect neurodegeneration or cognitive performance.
Wed Jun 05 00:00:00 EDT 2019
Revisiting the initial steps of sexual development in the malaria parasite Plasmodium falciparum.
Nature microbiology
Bancells C,Llorà-Batlle O,Poran A,Nötzel C,Rovira-Graells N,Elemento O,Kafsack BFC,Cortés A
A-11122 was used in Immunocytochemistry-immunoflourescence to study the initial steps of gametocytogenesis in Plasmodium falciparum.
Tue Jan 01 00:00:00 EST 2019
Amyloid Peptide Induced Neuroinflammation Increases the P2X7 Receptor Expression in Microglial Cells, Impacting on Its Functionality.
Frontiers in cellular neuroscience
Martínez-Frailes C,Di Lauro C,Bianchi C,de Diego-García L,Sebastián-Serrano Á,Boscá L,Díaz-Hernández M
A-11122 was used in Immunohistochemistry-immunofluorescence to evaluate whether the P2X7R age related-changes on microglia cells may be relevant to the alzheimer disease (AD) progression, by generating a new transgenic mouse model crossing an AΒ peptide mouse model, J20 mice and the P2X7R reporter mice P2X7REGFP.
Thu Oct 01 00:00:00 EDT 2020
Astroglial-targeted expression of the fragile X CGG repeat premutation in mice yields RAN translation, motor deficits and possible evidence for cell-to-cell propagation of FXTAS pathology.
Acta neuropathologica communications
Wenzel HJ,Murray KD,Haify SN,Hunsaker MR,Schwartzer JJ,Kim K,La Spada AR,Sopher BL,Hagerman PJ,Raske C,Severijnen LWFM,Willemsen R,Hukema RK,Berman RF
A-11122 was used in Immunocytochemistry to show that Gfa2-CGG99 expression in mice is sufficient to induce key features of FXTAS pathology, including formation of intranuclear inclusions, translation of FMRpolyG, and deficits in motor function.
Tue Feb 26 00:00:00 EST 2019
Drosophila Homeodomain-Interacting Protein Kinase (Hipk) Phosphorylates the Homeodomain Proteins Homeobrain, Empty Spiracles, and Muscle Segment Homeobox.
International journal of molecular sciences
Steinmetz EL,Dewald DN,Luxem N,Walldorf U
A-11122 was used in Immunohistochemistry-immunofluorescence to uncover a physical interaction between HipK and the three homeodomain proteins in vivo using bimolecular fluorescence complementation.
Fri Apr 19 00:00:00 EDT 2019
Lack of Fractalkine Receptor on Macrophages Impairs Spontaneous Recovery of Ribbon Synapses After Moderate Noise Trauma in C57BL/6 Mice.
Frontiers in neuroscience
Kaur T,Clayman AC,Nash AJ,Schrader AD,Warchol ME,Ohlemiller KK
A-11122 was used in Immunohistochemistry to study the role of macrophage fractalkine signalling on the repair of damaged synapses.
Wed Sep 30 00:00:00 EDT 2020
Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing.
eLife
Yang F,Quan Z,Huang H,He M,Liu X,Cai T,Xi R
A-11122 was used in Immunohistochemistry to provide a molecular connection between HP1a and local H3K4 demethylation during HP1a-mediated gene silencing that is required for ovary development, transposon silencing, and heterochromatin formation.
Wed Jan 16 00:00:00 EST 2019
Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing.
eLife
Yang F,Quan Z,Huang H,He M,Liu X,Cai T,Xi R
A-11122 was used in Immunohistochemistry to provide a molecular connection between HP1a and local H3K4 demethylation during HP1a-mediated gene silencing that is required for ovary development, transposon silencing, and heterochromatin formation.
Wed Jan 16 00:00:00 EST 2019
Transfer of Functional Cargo in Exomeres.
Cell reports
Zhang Q,Higginbotham JN,Jeppesen DK,Yang YP,Li W,McKinley ET,Graves-Deal R,Ping J,Britain CM,Dorsett KA,Hartman CL,Ford DA,Allen RM,Vickers KC,Liu Q,Franklin JL,Bellis SL,Coffey RJ
A-11122 was used in Immunocytochemistry to identify distinct functions of exomeres mediated by two of their cargo, the Β-galactoside α2,6-sialyltransferase 1 (ST6Gal-I) that α2,6- sialylates N-glycans, and the EGFR ligand, amphiregulin (AREG).
Tue Apr 16 00:00:00 EDT 2019
Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile.
Frontiers in immunology
Coronado M,Solis CJ,Hernandez PP,Feijóo CG
A-11122 was used in Immunohistochemistry to develop and assess the effectiveness of a novel anti-CD38 antibody, SG003, against CD38-related cancers.
Wed Jul 08 00:00:00 EDT 2020
Capicua regulates neural stem cell proliferation and lineage specification through control of Ets factors.
Nature communications
Ahmad ST,Rogers AD,Chen MJ,Dixit R,Adnani L,Frankiw LS,Lawn SO,Blough MD,Alshehri M,Wu W,Marra MA,Robbins SM,Cairncross JG,Schuurmans C,Chan JA
A-11122 was used in Immunohistochemistry-immunofluorescence to demonstrate that forebrain-specific Cic deletion increases proliferation and self-renewal of neural stem cells.
Wed May 01 00:00:00 EDT 2019
N-cadherin-regulated FGFR ubiquitination and degradation control mammalian neocortical projection neuron migration.
eLife
Kon E,Calvo-Jiménez E,Cossard A,Na Y,Cooper JA,Jossin Y
A-11122 was used in Western Blotting to reveal novel functions for FGFRs in cortical projection neuron migration, suggest a physiological role for FGFR and N-Cadherin interaction in vivo and identify Reelin as an extracellular upstream regulator and Erk1/2 as downstream effectors of FGFRs during neuron migration.
Wed Oct 02 00:00:00 EDT 2019
The yellow gene influences Drosophila male mating success through sex comb melanization.
eLife
Massey JH,Chung D,Siwanowicz I,Stern DL,Wittkopp PJ
A-11122 was used in Immunohistochemistry to show that the yellow gene in Drosophila melanogaster males effects pigmentation of sex combs and in turn mating success.
Tue Oct 15 00:00:00 EDT 2019
Notch and Delta are required for survival of the germline stem cell lineage in testes of Drosophila melanogaster.
PloS one
Ng CL,Qian Y,Schulz C
A-11122 was used in Immunohistochemistry-immunofluorescence to show that Notch and Delta are essential for germline stem cell lineage survival in Drosophila melanogaster.
Fri Mar 06 00:00:00 EST 2020
Auxin-sensitive Aux/IAA proteins mediate drought tolerance in Arabidopsis by regulating glucosinolate levels.
Nature communications
Salehin M,Li B,Tang M,Katz E,Song L,Ecker JR,Kliebenstein DJ,Estelle M
A-11122 was used in Chromatin immunoprecipitation to describe a mechanism by which auxin-sensitive Aux/IAA genes promote drought tolerance in Arabidopsis through the regulation of glucosinolate levels.
Fri Sep 06 00:00:00 EDT 2019
Mild Inactivation of RE-1 Silencing Transcription Factor (REST) Reduces Susceptibility to Kainic Acid-Induced Seizures.
Frontiers in cellular neuroscience
Carminati E,Buffolo F,Rocchi A,Michetti C,Cesca F,Benfenati F
A-11122 was used in Immunocytochemistry to study the impact of REST modulation on seizure propensity ,and developed a tool for its negative modulation in vivo.
Mon Sep 28 00:00:00 EDT 2020
Mild Inactivation of RE-1 Silencing Transcription Factor (REST) Reduces Susceptibility to Kainic Acid-Induced Seizures.
Frontiers in cellular neuroscience
Carminati E,Buffolo F,Rocchi A,Michetti C,Cesca F,Benfenati F
A-11122 was used in Immunocytochemistry to study the impact of REST modulation on seizure propensity ,and developed a tool for its negative modulation in vivo.
Mon Sep 28 00:00:00 EDT 2020
Glial Ca2+signaling links endocytosis to K+ buffering around neuronal somas to regulate excitability.
eLife
Weiss S,Melom JE,Ormerod KG,Zhang YV,Littleton JT
A-11122 was used in Western Blotting to show that elevation of glial Ca2+ causes hyperactivation of calcineurin-dependent endocytosis and accumulation of early endosomes.
Fri Apr 26 00:00:00 EDT 2019
Hes1 expression in mature neurons in the adult mouse brain is required for normal behaviors.
Scientific reports
Matsuzaki T,Yoshihara T,Ohtsuka T,Kageyama R
A-11122 was used in Immunohistochemistry-immunofluorescence to indicate that Hes1 is functionally important for normal behaviors not only in excitatory neurons but also in inhibitory neurons in the adult brain.
Mon Jun 03 00:00:00 EDT 2019
The ion channel ppk301 controls freshwater egg-laying in the mosquito Aedes aegypti.
eLife
Matthews BJ,Younger MA,Vosshall LB
A-11122 was used in Immunohistochemistry-immunofluorescence to suggest the role of ppk301 in egg-laying at different salt concentrations for the female Aedes mosquito.
Tue May 21 00:00:00 EDT 2019
CRISPR-Cas9 corrects Duchenne muscular dystrophy exon 44 deletion mutations in mice and human cells.
Science advances
Min YL,Li H,Rodriguez-Caycedo C,Mireault AA,Huang J,Shelton JM,McAnally JR,Amoasii L,Mammen PPA,Bassel-Duby R,Olson EN
A-11122 was used in Western Blotting to correct the mutations that delete exon 33 in dystrophin gene in mouse and human cells that eventually cause development of Duchenne muscular dystrophy.
Fri Mar 01 00:00:00 EST 2019
Proximity interactions of the ubiquitin ligase Mind bomb 1 reveal a role in regulation of epithelial polarity complex proteins.
Scientific reports
Dho SE,Silva-Gagliardi N,Morgese F,Coyaud E,Lamoureux E,Berry DM,Raught B,McGlade CJ
A-11122 was used in Immunocytochemistry-immunoflourescence to examine the role of Parkin in the context of mitochondrial DNA (mtDNA) damage and myocardial aging using a mouse model carrying a proofreading-defective mtDNA polymerase γ (POLG).
Wed Aug 28 00:00:00 EDT 2019
Robo2 regulates synaptic oxytocin content by affecting actin dynamics.
eLife
Anbalagan S,Blechman J,Gliksberg M,Gordon L,Rotkopf R,Dadosh T,Shimoni E,Levkowitz G
A-11122 was used in Immunohistochemistry-immunofluorescence to link Slit3-Robo2-Cdc42 which controls local actin dynamics with the maintenance of synaptic neuropeptide levels.
Mon Jun 10 00:00:00 EDT 2019
mTORC1 Signaling Is Palmitoylation-Dependent in Hippocampal Neurons and Non-neuronal Cells and Involves Dynamic Palmitoylation of LAMTOR1 and mTOR.
Frontiers in cellular neuroscience
Sanders SS,De Simone FI,Thomas GM
A-11122 was used in Western Blotting to determine the mechanism underlying the regulation of mTORC1 membrane targeting.
Tue Sep 29 00:00:00 EDT 2020
Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection.
Scientific reports
Schneider A,Kurz S,Manske K,Janas M,Heikenwälder M,Misgeld T,Aichler M,Weissmann SF,Zischka H,Knolle P,Wohlleber D
A-11122 was used in Immunohistochemistry to report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry.
Tue Jun 11 00:00:00 EDT 2019
RNAi-mediated depletion of the NSL complex subunits leads to abnormal chromosome segregation and defective centrosome duplication in Drosophila mitosis.
PLoS genetics
Pavlova GA,Popova JV,Andreyeva EN,Yarinich LA,Lebedev MO,Razuvaeva AV,Dubatolova TD,Oshchepkova AL,Pellacani C,Somma MP,Pindyurin AV,Gatti M
A-11122 was used in Immunocytochemistry-immunoflourescence to suggest that the mitotic phenotypes caused by Rcd1, Rcd5, MBD-R2 or Wds depletion are primarily due to reduced transcription of genes involved in kinetochore assembly and centriole duplication.
Sun Sep 01 00:00:00 EDT 2019
Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3.
eLife
Jia R,Bonifacino JS
A-11122 was used in Western Blot to demonstrate that UBA6 and BIRC6 negatively regulate autophagy by limiting the availability of LC3B.
Wed Nov 06 00:00:00 EST 2019
Differential interactions of missing in metastasis and insulin receptor tyrosine kinase substrate with RAB proteins in the endocytosis of CXCR4.
The Journal of biological chemistry
Li L,Baxter SS,Zhao P,Gu N,Zhan X
A-11122 was used in Immunocytochemistry-immunoflourescence to provide first evidence that the SH3 domain is critical in the regulation of specific endocytic pathways by I-BAR domain proteins.
Fri Apr 19 00:00:00 EDT 2019
Crumbs organizes the transport machinery by regulating apical levels of PI(4,5)P2 in Drosophila.
eLife
Lattner J,Leng W,Knust E,Brankatschk M,Flores-Benitez D
A-11122 was used in Western Blot to identify Crumbs as an essential hub required to maintain the organization of the apical membrane and the physiological activity of the larval salivary gland.
Thu Nov 07 00:00:00 EST 2019
Structures of CENP-C cupin domains at regional centromeres reveal unique patterns of dimerization and recruitment functions for the inner pocket.
The Journal of biological chemistry
Chik JK,Moiseeva V,Goel PK,Meinen BA,Koldewey P,An S,Mellone BG,Subramanian L,Cho US
A-11122 was used in Immunocytochemistry-immunoflourescence to unveil the evolutionarily conserved and unique features of the CENP-C cupin domain and uncover the mechanism by which it functions as a recruitment factor.
Fri Sep 20 00:00:00 EDT 2019
Investigation of the Developmental Requirements of Drosophila HP1 and Insulator Protein Partner, HIPP1.
G3 (Bethesda, Md.)
Glenn SE,Geyer PK
A-11122 was used in Immunohistochemistry to show that HIPP1 is a widely expressed nuclear protein that is dispensable for viability, as well as female and male fertility.
Thu Feb 07 00:00:00 EST 2019
scRNA-Seq reveals distinct stem cell populations that drive hair cell regeneration after loss of Fgf and Notch signaling.
eLife
Lush ME,Diaz DC,Koenecke N,Baek S,Boldt H,St Peter MK,Gaitan-Escudero T,Romero-Carvajal A,Busch-Nentwich EM,Perera AG,Hall KE,Peak A,Haug JS,Piotrowski T
A-11122 was used in Immunohistochemistry to study signalling patterns which trigger hair cell production in mammals by use of scRNA-Seq analysis.
Fri Jan 25 00:00:00 EST 2019
Maintenance of cell type-specific connectivity and circuit function requires Tao kinase.
Nature communications
Tenedini FM,Sáez González M,Hu C,Pedersen LH,Petruzzi MM,Spitzweck B,Wang D,Richter M,Petersen M,Szpotowicz E,Schweizer M,Sigrist SJ,Calderon de Anda F,Soba P
A-11122 was used in Immunohistochemistry to investigate, using a Drosophila model, the role of Tao kinase in regulating neuron development and circuit specificity and function.
Mon Aug 05 00:00:00 EDT 2019
The prion-like domain of Drosophila Imp promotes axonal transport of RNP granules in vivo.
Nature communications
Vijayakumar J,Perrois C,Heim M,Bousset L,Alberti S,Besse F
A-11122 was used in Immunohistochemistry-immunofluorescence to show that prion-like domains of Dropshila Imp is essential for developmentally-controlled localisation of Imp RNP granules to axons and regulates in vivo axonal remodeling.
Thu Jun 13 00:00:00 EDT 2019
Glycolysis upregulation is neuroprotective as a compensatory mechanism in ALS.
eLife
Manzo E,Lorenzini I,Barrameda D,O'Conner AG,Barrows JM,Starr A,Kovalik T,Rabichow BE,Lehmkuhl EM,Shreiner DD,Joardar A,Liévens JC,Bowser R,Sattler R,Zarnescu DC
A-11122 was used in Western Blotting to find significant alterations in glucose metabolism including increased pyruvate, suggesting that modulating glycolysis may be neuroprotective.
Mon Jun 10 00:00:00 EDT 2019
Stereotyped Combination of Hearing and Wind/Gravity-Sensing Neurons in the Johnston's Organ of Drosophila.
Frontiers in physiology
Ishikawa Y,Fujiwara M,Wong J,Ura A,Kamikouchi A
A-11122 was used in Immunohistochemistry to show that Johnston's organ neurons are grouped in stereotypical combinations each with a distinct response property in a scolopidium.
Wed Sep 30 00:00:00 EDT 2020
Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord.
Cell reports
Gorla M,Santiago C,Chaudhari K,Layman AAK,Oliver PM,Bashaw GJ
A-11122 was used in Immunohistochemistry to define a key role for the Ndfip adaptor proteins in preventing the surface expression of the Robo1 receptor prior to midline crossing.
Tue Mar 19 00:00:00 EDT 2019
Drosulfakinin signaling in fruitless circuitry antagonizes P1 neurons to regulate sexual arousal in Drosophila.
Nature communications
Wu S,Guo C,Zhao H,Sun M,Chen J,Han C,Peng Q,Qiao H,Peng P,Liu Y,Luo SD,Pan Y
A-11122 was used in Immunohistochemistry to identify the Cholecystokinin-like peptide Drosulfakinin that functions at single-neuron resolution to suppress male sexual behaviour in Drosophila.
Fri Oct 18 00:00:00 EDT 2019
Differential Nicotinic Modulation of Glutamatergic and GABAergic VTA Microcircuits.
eNeuro
Yan Y,Beckley NA,Kim VJ,Drenan RM
A-11122 was used in Immunohistochemistry to uncover a complex system of local circuitry in the VTA that is modulated by nAChR activity.
Wed Jun 03 00:00:00 EDT 2020
A regulatory role of autophagy for resetting the memory of heat stress in plants.
Plant, cell & environment
Sedaghatmehr M,Thirumalaikumar VP,Kamranfar I,Marmagne A,Masclaux-Daubresse C,Balazadeh S
A-11122 was used in Western Blotting to reveal a novel regulatory mechanism for heat stress memory in plants.
Fri Mar 01 00:00:00 EST 2019
Genetic deletion of genes in the cerebellar rhombic lip lineage can stimulate compensation through adaptive reprogramming of ventricular zone-derived progenitors.
Neural development
Wojcinski A,Morabito M,Lawton AK,Stephen DN,Joyner AL
A-11122 was used in Immunohistochemistry to suggest that the ability of the neonatal cerebellum to regenerate after loss of cells via a response by NEPs must therefore be considered when interpreting the phenotypes of Atoh1-Cre conditional mutants affecting GCPs.
Thu Feb 14 00:00:00 EST 2019
Direct visualization of a molecular handshake that governs kin recognition and tissue formation in myxobacteria.
Nature communications
Cao P,Wall D
A-11122 was used in Western Blotting to study how microbes use a fluid outer membrane receptor to recognize and assemble kin cells into a cooperative multicellular community that resembles a tissue.
Fri Jul 12 00:00:00 EDT 2019
Sniffer cells for the detection of neural Angiotensin II in vitro.
Scientific reports
Farmer GE,Amune A,Bachelor ME,Duong P,Yuan JP,Cunningham JT
A-11122 was used in Western Blotting to show that stable sniffer cell lines may be a viable method for detecting neuropeptide release in vitro, while still being able to distinguish differences in neuropeptide concentration.
Wed Jun 19 00:00:00 EDT 2019
A Role for Drosophila Amyloid Precursor Protein in Retrograde Trafficking of L1-Type Cell Adhesion Molecule Neuroglian.
Frontiers in cellular neuroscience
Penserga T,Kudumala SR,Poulos R,Godenschwege TA
A-11122 was used in Immunohistochemistry to characterize the role of the Drosophila homolog in the adult giant fiber neurons.
Wed Sep 30 00:00:00 EDT 2020
The Hox transcription factor Ubx stabilizes lineage commitment by suppressing cellular plasticity in Drosophila.
eLife
Domsch K,Carnesecchi J,Disela V,Friedrich J,Trost N,Ermakova O,Polychronidou M,Lohmann I
A-11122 was used in Western Blotting to study the mechanism by which alternative fate genes are lineage specifically silenced.
Fri May 03 00:00:00 EDT 2019
Stress-induced phospho-ubiquitin formation causes parkin degradation.
Scientific reports
Kovalchuke L,Mosharov EV,Levy OA,Greene LA
A-11122 was used in Immunocytochemistry to determine the mechanisms by which stress decreases parkin protein levels using cultured neuronal cells and the PD-relevant stressor, L-DOPA.
Mon Aug 12 00:00:00 EDT 2019
Cytoskeletal tension and Bazooka tune interface geometry to ensure fusion fidelity and sheet integrity during dorsal closure.
eLife
Das Gupta PT,Narasimha M
A-11122 was used in Immunohistochemistry to demonstrate that epidermal flanks modulate cell numbers and geometry of their fusing fronts to achieve fusion fidelity.
Wed Apr 17 00:00:00 EDT 2019
Upregulation of cancer-associated gene expression in activated fibroblasts in a mouse model of non-alcoholic steatohepatitis.
Scientific reports
Asakawa M,Itoh M,Suganami T,Sakai T,Kanai S,Shirakawa I,Yuan X,Hatayama T,Shimada S,Akiyama Y,Fujiu K,Inagaki Y,Manabe I,Yamaoka S,Yamada T,Tanaka S,Ogawa Y
A-11122 was used in Immunohistochemistry to reveal upregulation of cancer-associated gene expression in activated fibroblasts in NASH, which would contribute to the progression from NASH to HCC.
Fri Dec 20 00:00:00 EST 2019
Dental Epithelial Stem Cells as a Source for Mammary Gland Regeneration and Milk Producing Cells In Vivo.
Cells
Jimenez-Rojo L,Pagella P,Harada H,Mitsiadis TA
A-11122 was used in Immunohistochemistry to study how alterations in the microenvironment of dental epithelial stem cells can lead to the regeneration of non-dental tissues.
Tue Oct 22 00:00:00 EDT 2019
Activity-Dependent Reconnection of Adult-Born Dentate Granule Cells in a Mouse Model of Frontotemporal Dementia.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Terreros-Roncal J,Flor-García M,Moreno-Jiménez EP,Pallas-Bazarra N,Rábano A,Sah N,van Praag H,Giacomini D,Schinder AF,Ávila J,Llorens-Martín M
A-11122 was used in Immunohistochemistry to study the therapeutic potential of applying chemoactivation to adult-born dentate granule cells in a mouse model of a neurodegenerative disease.
Wed Jul 17 00:00:00 EDT 2019
E2F4 regulates transcriptional activation in mouse embryonic stem cells independently of the RB family.
Nature communications
Hsu J,Arand J,Chaikovsky A,Mooney NA,Demeter J,Brison CM,Oliverio R,Vogel H,Rubin SM,Jackson PK,Sage J
A-11122 was used in Western Blotting to show that E2F4 is important for the proliferation and the survival of mouse embryonic stem cells.
Wed Jul 03 00:00:00 EDT 2019
Interspecies conservation of organisation and function between nonhomologous regional centromeres.
Nature communications
Tong P,Pidoux AL,Toda NRT,Ard R,Berger H,Shukla M,Torres-Garcia J,Müller CA,Nieduszynski CA,Allshire RC
A-11122 was used in Immunocytochemistry-immunoflourescence to show that despite the lack of sequence conservation, Schizosaccharomyces centromere DNA possesses intrinsic conserved properties that promote assembly of CENP-A chromatin.
Tue May 28 00:00:00 EDT 2019
Cellular properties of intrinsically photosensitive retinal ganglion cells during postnatal development.
Neural development
Lucas JA,Schmidt TM
A-11122 was used in Immunohistochemistry to show that ipRGC subtypes are distinguishable from each other early in postnatal development.
Fri Aug 30 00:00:00 EDT 2019
Grafted Human iPSC-Derived Neural Progenitor Cells Express Integrins and Extend Long-Distance Axons Within the Developing Corticospinal Tract.
Frontiers in cellular neuroscience
Forbes LH,Andrews MR
A-11122 was used in Western Blotting to study if human induced pluripotent stem cell (iPSC)-derived neural progenitor cells (NPCs) increase expression of α9 integrin within developing rat corticospinal tract (CST).
Thu Oct 01 00:00:00 EDT 2020
Grafted Human iPSC-Derived Neural Progenitor Cells Express Integrins and Extend Long-Distance Axons Within the Developing Corticospinal Tract.
Frontiers in cellular neuroscience
Forbes LH,Andrews MR
A-11122 was used in Western Blotting to study if human induced pluripotent stem cell (iPSC)-derived neural progenitor cells (NPCs) increase expression of α9 integrin within developing rat corticospinal tract (CST).
Thu Oct 01 00:00:00 EDT 2020
Grafted Human iPSC-Derived Neural Progenitor Cells Express Integrins and Extend Long-Distance Axons Within the Developing Corticospinal Tract.
Frontiers in cellular neuroscience
Forbes LH,Andrews MR
A-11122 was used in Western Blotting to study if human induced pluripotent stem cell (iPSC)-derived neural progenitor cells (NPCs) increase expression of α9 integrin within developing rat corticospinal tract (CST).
Thu Oct 01 00:00:00 EDT 2020
Loss of PGC-1α in RPE induces mesenchymal transition and promotes retinal degeneration.
Life science alliance
Rosales MAB,Shu DY,Iacovelli J,Saint-Geniez M
A-11122 was used in Immunohistochemistry to establish that PGC-1α is required to maintain the functional and phenotypical status of retinal pigment epithelium by supporting the cells'' oxidative metabolism and autography-mediated repression of epithelial to mesenchymal transition.
Sat Jun 01 00:00:00 EDT 2019
Midbrain tectal stem cells display diverse regenerative capacities in zebrafish.
Scientific reports
Lindsey BW,Aitken GE,Tang JK,Khabooshan M,Douek AM,Vandestadt C,Kaslin J
A-11122 was used in Immunohistochemistry to assess the function of quiescent RG (qRG) during regeneration by performing a stab lesion in the adult midbrain tectum to target a population of homeostatic qRG, and to investigate their proliferative behaviour, differentiation potential, and Wnt/Β-catenin signalling.
Thu Mar 14 00:00:00 EDT 2019
Differential regulation of the Drosophila sleep homeostat by circadian and arousal inputs.
eLife
Ni JD,Gurav AS,Liu W,Ogunmowo TH,Hackbart H,Elsheikh A,Verdegaal AA,Montell C
A-11122 was used in Immunohistochemistry to propose that dFB neurons integrate synaptic inputs from distinct sets of upstream sleep-promoting circadian clock neurons, and arousal neurons.
Tue Feb 05 00:00:00 EST 2019
Essential role for InSyn1 in dystroglycan complex integrity and cognitive behaviors in mice.
eLife
Uezu A,Hisey E,Kobayashi Y,Gao Y,Bradshaw TW,Devlin P,Rodriguiz R,Tata PR,Soderling S
A-11122 was used in Western Blotting to report Inhibitory Synaptic protein 1 (InSyn1) is a critical component of the DGC whose loss alters the composition of the GABAergic synapses, excitatory/inhibitory balance in vitro and in vivo, and cognitive behavior.
Thu Dec 12 00:00:00 EST 2019
Cardiomyocyte Progenitor Cells as a Functional Gene Delivery Vehicle for Long-Term Biological Pacing.
Molecules (Basel, Switzerland)
Végh AMD,den Haan AD,Cócera Ortega L,Verkerk AO,Sluijter JPG,Bakker D,van Amersfoorth S,van Veen TAB,Klerk M,Seppen J,de Bakker JMT,Christoffels VM,Geerts D,Goumans MJTH,Tan HL,Boink GJJ
A-11122 was used in Immunocytochemistry-immunoflourescence to show that cardiomyocyte progenitor cells expressing HCN4-GFP functionally couple to neonatal rat ventricular myocytes and induce physiologically controlled pacemaker activity.
Sat Jan 05 00:00:00 EST 2019
Ceapins block the unfolded protein response sensor ATF6α by inducing a neomorphic inter-organelle tether.
eLife
Torres SE,Gallagher CM,Plate L,Gupta M,Liem CR,Guo X,Tian R,Stroud RM,Kampmann M,Weissman JS,Walter P
A-11122 was used in Western Blotting to reveal that Ceapins function by chemical-induced misdirection.
Fri May 31 00:00:00 EDT 2019
Leaky expression of channelrhodopsin-2 (ChR2) in Ai32 mouse lines.
PloS one
Prabhakar A,Vujovic D,Cui L,Olson W,Luo W
A-11122 was used in Immunoprecipitation to study leaky 'off-target' (Cre-independent) ChR2-EYFP expression in Ai32/Ai32 homozygous mice.
Fri Dec 13 00:00:00 EST 2019
Classes of non-conventional tetraspanins defined by alternative splicing.
Scientific reports
Hochheimer N,Sies R,Aschenbrenner AC,Schneider D,Lang T
A-11122 was used in Western Blotting to analyse alternative splicing of tetraspanins.
Tue Oct 01 00:00:00 EDT 2019
Downregulation of Notch Signaling in Kras-Induced Gastric Metaplasia.
Neoplasia (New York, N.Y.)
Chung WC,Zhou Y,Atfi A,Xu K
A-11122 was used in Immunohistochemistry to reveal downregulation of Notch signalling and Mist1 expression during the initiation of Kras-driven gastric tumourigenesis.
Thu Aug 01 00:00:00 EDT 2019
Tumorigenic effects of TLX overexpression in HEK 293T cells.
Cancer reports (Hoboken, N.J.)
Parris TZ,Vizlin-Hodzic D,Salmela S,Funa K
A-11122 was used in Immunocytochemistry to illustrate that TLX may play a role in tumorigenesis via genomic instability and perturbation of cancer-related processes.
Tue Oct 01 00:00:00 EDT 2019
A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission.
Neuron
Liu Q,Sinnen BL,Boxer EE,Schneider MW,Grybko MJ,Buchta WC,Gibson ES,Wysoczynski CL,Ford CP,Gottschalk A,Aoto J,Tucker CL,Kennedy MJ
A-11122 was used in Western Blotting to demonstrate the utility of this approach for inducibly disrupting excitatory neurotransmission, providing a first-in-class optogenetic tool for persistent, light-triggered synaptic inhibition.
Wed Mar 06 00:00:00 EST 2019
Identification of a bilirubin receptor that may mediate a component of cholestatic itch.
eLife
Meixiong J,Vasavda C,Green D,Zheng Q,Qi L,Kwatra SG,Hamilton JP,Snyder SH,Dong X
A-11122 was used in Immunohistochemistry to show that genetic deletion of either Mrgpra1 or Blvra, the gene that encodes the bilirubin-producing enzyme biliverdin reductase, attenuates itch.
Mon Jan 21 00:00:00 EST 2019
A meiosis-specific BRCA2 binding protein recruits recombinases to DNA double-strand breaks to ensure homologous recombination.
Nature communications
Zhang J,Fujiwara Y,Yamamoto S,Shibuya H
A-11122 was used in Immunocytochemistry-immunoflourescence to study the role of BRCA2 in the repair mechanism of double stranded breaks.
Wed Feb 13 00:00:00 EST 2019
Functional Characterization of Clinically-Relevant Rare Variants in ABCG2 Identified in a Gout and Hyperuricemia Cohort.
Cells
Toyoda Y,Mančíková A,Krylov V,Morimoto K,Pavelcová K,Bohatá J,Pavelka K,Pavlíková M,Suzuki H,Matsuo H,Takada T,Stiburkova B
A-11122 was used in Western Blotting to contribute to deepening the understanding of ABCG2-related gout/hyperuricemia risk and the biochemical characteristics of the ABCG2 protein.
Thu Apr 18 00:00:00 EDT 2019
Membrane-bound Gaussia luciferase as a tool to track shedding of membrane proteins from the surface of extracellular vesicles.
Scientific reports
Zaborowski MP,Cheah PS,Zhang X,Bushko I,Lee K,Sammarco A,Zappulli V,Maas SLN,Allen RM,Rumde P,György B,Aufiero M,Schweiger MW,Lai CP,Weissleder R,Lee H,Vickers KC,Tannous BA,Breakefield XO
A-11122 was used in Western Blotting to develop the membrane embedded Gaussia luciferase as a marker for membrane protein loss and show ectodomain shedding from extracellular vesicles is continuous and mediated by proteases.
Fri Nov 22 00:00:00 EST 2019
Neurons in the Nucleus papilio contribute to the control of eye movements during REM sleep.
Nature communications
Gutierrez Herrera C,Girard F,Bilella A,Gent TC,Roccaro-Waldmeyer DM,Adamantidis A,Celio MR
A-11122 was used in Immunohistochemistry to provide the first evidence for a brainstem premotor command contributing to the control of eye movements selectively during REM sleep in the mammalian brain.
Tue Nov 19 00:00:00 EST 2019
A projection specific logic to sampling visual inputs in mouse superior colliculus.
eLife
Reinhard K,Li C,Do Q,Burke EG,Heynderickx S,Farrow K
A-11122 was used in Immunohistochemistry to open the possibility that projection-specific sampling of retinal inputs forms a basis for the selective triggering of behaviors by the superior colliculus.
Thu Nov 21 00:00:00 EST 2019
M1 Intrinsically Photosensitive Retinal Ganglion Cells Integrate Rod and Melanopsin Inputs to Signal in Low Light.
Cell reports
Lee SK,Sonoda T,Schmidt TM
A-11122 was used in Immunohistochemistry to show variability in the rod input to M1 ipRGCs and a surprising contribution of melanopsin to the light responses of M1 ipRGCs at very low light.
Tue Dec 10 00:00:00 EST 2019
CENP-A Ubiquitylation Is Indispensable to Cell Viability.
Developmental cell
Niikura Y,Kitagawa R,Fang L,Kitagawa K
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation to show that the Flag-tagged or untagged CENP-A K124R mutant is lethal.
Mon Sep 23 00:00:00 EDT 2019
Cerebellar folding is initiated by mechanical constraints on a fluid-like layer without a cellular pre-pattern.
eLife
Lawton AK,Engstrom T,Rohrbach D,Omura M,Turnbull DH,Mamou J,Zhang T,Schwarz JM,Joyner AL
A-11122 was used in Immunohistochemistry to combine existing models with in vivo data collected from mouse cerebellum, to show how cerebellar folding emerges.
Tue Apr 16 00:00:00 EDT 2019
Ablation of glucocorticoid receptor in the hindbrain of the mouse provides a novel model to investigate stress disorders.
Scientific reports
Gannon AL,O'Hara L,Mason JI,Rebourcet D,Smith S,Traveres A,Alcaide-Corral CJ,Frederiksen H,Jørgensen A,Milne L,Mitchell RT,Smith LB
A-11122 was used in Immunohistochemistry to investigate, using a novel mouse model, the effects of ablation of Glucocorticoid Receptor (GR) in the hindbrain, on stress-related behaviour and physiology.
Fri Mar 01 00:00:00 EST 2019
Auxin Response Factors promote organogenesis by chromatin-mediated repression of the pluripotency gene SHOOTMERISTEMLESS.
Nature communications
Chung Y,Zhu Y,Wu MF,Simonini S,Kuhn A,Armenta-Medina A,Jin R,Østergaard L,Gillmor CS,Wagner D
A-11122 was used in Chromatin immunoprecipitation to show that downregulation of the STM pluripotency gene promotes initiation of flowers and uncover mechanisms of STM silencing.
Thu Feb 21 00:00:00 EST 2019
AAV GCG-EGFP, a new tool to identify glucagon-secreting α-cells.
Scientific reports
Tudurí E,Glavas MM,Asadi A,Baker RK,Ellis CE,Soukhatcheva G,Philit M,Huynh FK,Johnson JD,Bruce Verchere C,Kieffer TJ
A-11122 was used in Immunohistochemistry-immunofluorescence to create a new double stranded adeno-associated virus AAV GCG-EGFP as a tool to identify primary glucagon-secreting α-cells.
Thu Jul 25 00:00:00 EDT 2019
Musclin, A Myokine Induced by Aerobic Exercise, Retards Muscle Atrophy During Cancer Cachexia in Mice.
Cancers
Re Cecconi AD,Forti M,Chiappa M,Zhu Z,Zingman LV,Cervo L,Beltrame L,Marchini S,Piccirillo R
A-11122 was used in Western Blotting to identify a myokine regulated by PGC1α and/or aerobic exercise, with possible therapeutic action, that could hopefully be confirmed in cancer patients who are not able to do regular physical activity.
Sat Oct 12 00:00:00 EDT 2019
Histone deposition promotes recombination-dependent replication at arrested forks.
PLoS genetics
Hardy J,Dai D,Ait Saada A,Teixeira-Silva A,Dupoiron L,Mojallali F,Fréon K,Ochsenbein F,Hartmann B,Lambert S
A-11122 was used in Immunoprecipitation to show recombination-dependent-replication is promoted by histone deposition.
Tue Oct 01 00:00:00 EDT 2019
Histone deposition promotes recombination-dependent replication at arrested forks.
PLoS genetics
Hardy J,Dai D,Ait Saada A,Teixeira-Silva A,Dupoiron L,Mojallali F,Fréon K,Ochsenbein F,Hartmann B,Lambert S
A-11122 was used in Immunoprecipitation to show recombination-dependent-replication is promoted by histone deposition.
Tue Oct 01 00:00:00 EDT 2019
A new transgenic reporter line reveals Wnt-dependent Snai2 re-expression and cranial neural crest differentiation in Xenopus.
Scientific reports
Li J,Perfetto M,Materna C,Li R,Thi Tran H,Vleminckx K,Duncan MK,Wei S
A-11122 was used in Immunohistochemistry to determine the role of snai2 in cranial neural crest differentiation in Xenopus.
Thu Aug 01 00:00:00 EDT 2019
Rapid single-wavelength lightsheet localization microscopy for clarified tissue.
Nature communications
Chu LA,Lu CH,Yang SM,Liu YT,Feng KL,Tsai YC,Chang WK,Wang WC,Chang SW,Chen P,Lee TK,Hwu YK,Chiang AS,Chen BC
A-11122 was used in Immunohistochemistry to develop a single-wavelength Bessel lightsheet method, optimized for refractive-index matching with clarified specimens to overcome the aberrations encountered in imaging thick tissues.
Fri Oct 18 00:00:00 EDT 2019
The Drosophila ERG channel seizure plays a role in the neuronal homeostatic stress response.
PLoS genetics
Hill AS,Jain P,Folan NE,Ben-Shahar Y
A-11122 was used in Immunohistochemistry-immunofluorescence to show the Drosophila voltage-gated potassium channel seizure contributes to neuronal homeostasis, robustness, and stress resistance.
Thu Aug 01 00:00:00 EDT 2019
A circadian output center controlling feeding:fasting rhythms in Drosophila.
PLoS genetics
Dreyer AP,Martin MM,Fulgham CV,Jabr DA,Bai L,Beshel J,Cavanaugh DJ
A-11122 was used in Immunohistochemistry to identify a non-clock cell neuronal population in Drosophila that regulates feeding rhythms and furthermore demonstrate dissociable control of circadian and homeostatic aspects of feeding regulation by molecularly-defined neurons in a putative circadian output hub.
Fri Nov 01 00:00:00 EDT 2019
Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forks.
Life science alliance
Ait-Saada A,Khorosjutina O,Chen J,Kramarz K,Maksimov V,Svensson JP,Lambert S,Ekwall K
A-11122 was used in Chromatin immunoprecipitation to identify that chromatin remodeler Fft3 resections nascent strands and restarts blocked DNA replication forks.
Tue Oct 01 00:00:00 EDT 2019
Exploring First Interactions Between Ostreid Herpesvirus 1 (OsHV-1) and Its Host, Crassostrea gigas: Effects of Specific Antiviral Antibodies and Dextran Sulfate.
Frontiers in microbiology
Martenot C,Faury N,Morga B,Degremont L,Lamy JB,Houssin M,Renault T
A-11122 was used in In vitro Assay to contribute to better understanding the pathogenesis of the viral infection, especially during the first stage of OsHV-1 infection, and open the way toward new approaches to control OsHV-1 infection in confined facilities.
Thu Oct 01 00:00:00 EDT 2020
Dentate granule cell recruitment of feedforward inhibition governs engram maintenance and remote memory generalization.
Nature medicine
Guo N,Soden ME,Herber C,Kim MT,Besnard A,Lin P,Ma X,Cepko CL,Zweifel LS,Sahay A
A-11122 was used in Immunohistochemistry to exemplify a connectivity-based strategy that targets a molecular brake of feedforward inhibition in DG-CA3 and may be harnessed to decrease time-dependent memory generalization in individuals with PTSD and improve memory precision in aging individuals.
Tue May 01 00:00:00 EDT 2018
In utero CRISPR-mediated therapeutic editing of metabolic genes.
Nature medicine
Rossidis AC,Stratigis JD,Chadwick AC,Hartman HA,Ahn NJ,Li H,Singh K,Coons BE,Li L,Lv W,Zoltick PW,Alapati D,Zacharias W,Jain R,Morrisey EE,Musunuru K,Peranteau WH
A-11122 was used in Immunohistochemistry to demonstrate the possibility of efficiently performing gene editing before birth, giving potential to new therapeutic approach for selected congenital genetic disorders.
Mon Oct 01 00:00:00 EDT 2018
Diametric neural ensemble dynamics in parkinsonian and dyskinetic states.
Nature
Parker JG,Marshall JD,Ahanonu B,Wu YW,Kim TH,Grewe BF,Zhang Y,Li JZ,Ding JB,Ehlers MD,Schnitzer MJ
A-11122 was used in Immunohistochemistry to show that both the spatiotemporal profiles and rates of SPN activity appear crucial to striatal function, and next-generation treatments for basal ganglia disorders should target both facets of striatal activity.
Tue May 01 00:00:00 EDT 2018
A Cdk9-PP1 switch regulates the elongation-termination transition of RNA polymerase II.
Nature
Parua PK,Booth GT,Sansó M,Benjamin B,Tanny JC,Lis JT,Fisher RP
A-11122 was used in Western Blotting to suggest that the transition of Pol II from elongation to termination coincides with a Dis2-dependent reversal of Cdk9 signalling-a switch that is analogous to a Cdk1-PP1 circuit that controls mitotic progression 4.
Fri Jun 01 00:00:00 EDT 2018
A synaptic threshold mechanism for computing escape decisions.
Nature
Evans DA,Stempel AV,Vale R,Ruehle S,Lefler Y,Branco T
A-11122 was used in Immunohistochemistry to show that dPAG glutamatergic neurons compute escape decisions and escape vigour using a synaptic mechanism to threshold threat information received from the mSC.
Fri Jun 01 00:00:00 EDT 2018
Brain-wide Electrical Spatiotemporal Dynamics Encode Depression Vulnerability.
Cell
Hultman R,Ulrich K,Sachs BD,Blount C,Carlson DE,Ndubuizu N,Bagot RC,Parise EM,Vu MT,Gallagher NM,Wang J,Silva AJ,Deisseroth K,Mague SD,Caron MG,Nestler EJ,Carin L,Dzirasa K
A-11122 was used in Immunohistochemistry to discover a spatiotemporal dynamic network that predicts the emergence of major depressive disorder (MDD)-related behavioral dysfunction in mice subjected to chronic social defeat stress.
Thu Mar 22 00:00:00 EDT 2018
A Neural Circuit for the Suppression of Pain by a Competing Need State.
Cell
Alhadeff AL,Su Z,Hernandez E,Klima ML,Phillips SZ,Holland RA,Guo C,Hantman AW,De Jonghe BC,Betley JN
A-11122 was used in Immunohistochemistry-immunofluorescence to determine the neural processes that mediate competing survival needs between hunger and pain.
Thu Mar 22 00:00:00 EDT 2018
Developmentally primed cortical neurons maintain fidelity of differentiation and establish appropriate functional connectivity after transplantation.
Nature neuroscience
Wuttke TV,Markopoulos F,Padmanabhan H,Wheeler AP,Murthy VN,Macklis JD
A-11122 was used in Immunohistochemistry-immunofluorescence to demonstrate that postnatal neocortical connectivity can be reconstituted with point-to-point precision, including cellular integration of specific, molecularly identified projection neuron subtypes into correct position, combined with development of appropriate long-distance projections and synapses.
Sun Apr 01 00:00:00 EDT 2018
Single-Cell Transcriptomic Analysis of Cardiac Differentiation from Human PSCs Reveals HOPX-Dependent Cardiomyocyte Maturation.
Cell stem cell
Friedman CE,Nguyen Q,Lukowski SW,Helfer A,Chiu HS,Miklas J,Levy S,Suo S,Han JJ,Osteil P,Peng G,Jing N,Baillie GJ,Senabouth A,Christ AN,Bruxner TJ,Murry CE,Wong ES,Ding J,Wang Y,Hudson J,Ruohola-Baker H,Bar-Joseph Z,Tam PPL,Powell JE,Palpant NJ
A-11122 was used in Western Blot to provide a key transcriptional roadmap of in vitro cardiac differentiation at single-cell resolution, revealing fundamental mechanisms underlying heart development and differentiation of hPSC-derived cardiomyocytes.
Thu Oct 04 00:00:00 EDT 2018
LHP1 Interacts with ATRX through Plant-Specific Domains at Specific Loci Targeted by PRC2.
Molecular plant
Wang H,Jiang D,Axelsson E,Lorković ZJ,Montgomery S,Holec S,Pieters BJGE,Al Temimi AHK,Mecinović J,Berger F
A-11122 was used in Western Blotting to suggest that distinct evolutionary pathways led to the interaction between ATRX and HP1 in mammals and its counterpart LHP1 in plants, resulting in distinct modes of transcriptional regulation.
Mon Aug 06 00:00:00 EDT 2018
A WW Domain-Containing Protein Forms Immune Nuclear Bodies against Begomoviruses.
Molecular plant
Calil IP,Quadros IPS,Araújo TC,Duarte CEM,Gouveia-Mageste BC,Silva JCF,Brustolini OJB,Teixeira RM,Oliveira CN,Milagres RWMM,Martins GS,Chory J,Reis PAB,Machado JPB,Fontes EPB
A-11122 was used in Chromatin immunoprecipitation to report the nuclear redistribution of NSP-interacting guanosine triphosphatase by AtWWP1, a WW domain-containing protein that forms immune nuclear bodies against begomoviruses.
Mon Dec 03 00:00:00 EST 2018
Role for VGLUT2 in selective vulnerability of midbrain dopamine neurons.
The Journal of clinical investigation
Steinkellner T,Zell V,Farino ZJ,Sonders MS,Villeneuve M,Freyberg RJ,Przedborski S,Lu W,Freyberg Z,Hnasko TS
A-11122 was used in Immunohistochemistry to suggest that the balance of VGLUT2 expression is a crucial determinant of DA neuron survival.
Thu Feb 01 00:00:00 EST 2018
Timing Mechanisms Underlying Gate Control by Feedforward Inhibition.
Neuron
Zhang Y,Liu S,Zhang YQ,Goulding M,Wang YQ,Ma Q
A-11122 was used in Immunohistochemistry-immunofluorescence to study how monosynaptic expression is gated by disynaptic inhibitory inputs.
Wed Sep 05 00:00:00 EDT 2018
A Hypothalamic Midbrain Pathway Essential for Driving Maternal Behaviors.
Neuron
Fang YY,Yamaguchi T,Song SC,Tritsch NX,Lin D
A-11122 was used in Immunohistochemistry-immunofluorescence to identify MPOAEsr1+ cells as an essential population for mediating maternal behaviors, especially pup approach and retrieval.
Wed Apr 04 00:00:00 EDT 2018
How Diverse Retinal Functions Arise from Feedback at the First Visual Synapse.
Neuron
Drinnenberg A,Franke F,Morikawa RK,Jüttner J,Hillier D,Hantz P,Hierlemann A,Azeredo da Silveira R,Roska B
A-11122 was used in Immunohistochemistry to reveal how a single interneuron type can differentially shape the dynamical properties of distinct output channels of a brain region.
Wed Jul 11 00:00:00 EDT 2018
FOXG1 Orchestrates Neocortical Organization and Cortico-Cortical Connections.
Neuron
Cargnin F,Kwon JS,Katzman S,Chen B,Lee JW,Lee SK
A-11122 was used in Immunocytochemistry, Immunohistochemistry, Western Blot to investigate molecular regulation of FOXG1 syndrome.
Wed Dec 05 00:00:00 EST 2018
Synaptic activity-induced glycolysis facilitates membrane lipid provision and neurite outgrowth.
The EMBO journal
Segarra-Mondejar M,Casellas-Díaz S,Ramiro-Pareta M,Müller-Sánchez C,Martorell-Riera A,Hermelo I,Reina M,Aragonés J,Martínez-Estrada OM,Soriano FX
A-11122 was used in Immunocytochemistry-immunoflourescence to suggest that the manipulation of neuronal glucose metabolism could be used to treat some brain developmental disorders.
Wed May 02 00:00:00 EDT 2018
Myc and the Tip60 chromatin remodeling complex control neuroblast maintenance and polarity in Drosophila.
The EMBO journal
Rust K,Tiwari MD,Mishra VK,Grawe F,Wodarz A
A-11122 was used in Immunohistochemistry to reveal an evolutionarily conserved functional link between Myc, the Tip60 complex, and the molecular network controlling cell polarity and asymmetric cell division.
Wed Aug 15 00:00:00 EDT 2018
Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma.
Clinical cancer research : an official journal of the American Association for Cancer Research
Gordon RE,Zhang L,Peri S,Kuo YM,Du F,Egleston BL,Ng JMY,Andrews AJ,Astsaturov I,Curran T,Yang ZJ
A-11122 was used in Immunohistochemistry to identify the important role for cholesterol in hedgehog pathway medulloblastoma (Hh-MB) tumorigenesis, and investigate the mechanisms by which statins aid the treatment of Hh-MB.
Thu Mar 15 00:00:00 EDT 2018
Selective activation of parvalbumin interneurons prevents stress-induced synapse loss and perceptual defects.
Molecular psychiatry
Chen CC,Lu J,Yang R,Ding JB,Zuo Y
A-11122 was used in Immunohistochemistry to suggest that the PV+ inhibitory circuit is crucial for normal synaptic dynamics in the mouse barrel cortex and sensory function.
Sun Jul 01 00:00:00 EDT 2018
The WAVE Regulatory Complex and Branched F-Actin Counterbalance Contractile Force to Control Cell Shape and Packing in the Drosophila Eye.
Developmental cell
Del Signore SJ,Cilla R,Hatini V
A-11122 was used in Immunohistochemistry-immunofluorescence to investigate the remodelling of Drosophila eye lattice cells which preserve cell contacts as they narrow to form the edges of a multicellular hexagonal lattice.
Mon Feb 26 00:00:00 EST 2018
Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair.
Developmental cell
Zhao C,Dong C,Frah M,Deng Y,Marie C,Zhang F,Xu L,Ma Z,Dong X,Lin Y,Koenig S,Nait-Oumesmar B,Martin DM,Wu LN,Xin M,Zhou W,Parras C,Lu QR
A-11122 was used in Western Blotting to demonstrate the dual function of CHD8 through inducing a cascade of chromatin reprogramming and recruiting H3K4 histone methyltransferases to establish oligodendrocyte identity.
Mon Jun 18 00:00:00 EDT 2018
Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair.
Developmental cell
Zhao C,Dong C,Frah M,Deng Y,Marie C,Zhang F,Xu L,Ma Z,Dong X,Lin Y,Koenig S,Nait-Oumesmar B,Martin DM,Wu LN,Xin M,Zhou W,Parras C,Lu QR
A-11122 was used in Western Blotting to demonstrate the dual function of CHD8 through inducing a cascade of chromatin reprogramming and recruiting H3K4 histone methyltransferases to establish oligodendrocyte identity.
Mon Jun 18 00:00:00 EDT 2018
Spliceosome Mutations Induce R Loop-Associated Sensitivity to ATR Inhibition in Myelodysplastic Syndromes.
Cancer research
Nguyen HD,Leong WY,Li W,Reddy PNG,Sullivan JD,Walter MJ,Zou L,Graubert TA
A-11122 was used in Flow cytometry/Cell sorting to study the altered RNA splicing caused by U2AF1 mutant accumulation and the consequential molecular effects.
Sat Sep 15 00:00:00 EDT 2018
In Vivo Selection of a Computationally Designed SCHEMA AAV Library Yields a Novel Variant for Infection of Adult Neural Stem Cells in the SVZ.
Molecular therapy : the journal of the American Society of Gene Therapy
Ojala DS,Sun S,Santiago-Ortiz JL,Shapiro MG,Romero PA,Schaffer DV
A-11122 was used in Immunohistochemistry to establish the SCHEMA library as a valuable tool for directed evolution and SCH9 as an effective gene delivery vector to investigate subventricular NSCs.
Wed Jan 03 00:00:00 EST 2018
Targeted Recruitment of the Basal Transcriptional Machinery by LNK Clock Components Controls the Circadian Rhythms of Nascent RNAs in Arabidopsis.
The Plant cell
Ma Y,Gil S,Grasser KD,Mas P
A-11122 was used in Western Blotting to explain how genome readout of environmental information ultimately results in rhythmic changes of gene expression.
Sun Apr 01 00:00:00 EDT 2018
Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death.
The Plant cell
Srivastava R,Li Z,Russo G,Tang J,Bi R,Muppirala U,Chudalayandi S,Severin A,He M,Vaitkevicius SI,Lawrence-Dill CJ,Liu P,Stapleton AE,Bassham DC,Brandizzi F,Howell SH
A-11122 was used in Chromatin immunoprecipitation to induce the unfolded protein response in maize seedlings to characterise the molecular events that occur over time during persistent ER stress.
Fri Jun 01 00:00:00 EDT 2018
GRF-interacting factor1 Regulates Shoot Architecture and Meristem Determinacy in Maize.
The Plant cell
Zhang D,Sun W,Singh R,Zheng Y,Cao Z,Li M,Lunde C,Hake S,Zhang Z
A-11122 was used in Chromatin immunoprecipitation to provide insights into the biological functions of gif1.
Thu Feb 01 00:00:00 EST 2018
Quantitative Analysis Reveals that Actin and Src-Family Kinases Regulate Nuclear YAP1 and Its Export.
Cell systems
Ege N,Dowbaj AM,Jiang M,Howell M,Hooper S,Foster C,Jenkins RP,Sahai E
A-11122 was used in Immunocytochemistry to investigate the dynamics of YAP1 shuttling in normal and activated fibroblasts, using EYFP-YAP1, quantitative photobleaching methods, and mathematical modeling. Imaging of migrating fibroblasts reveals the tight temporal coupling of cell shape change and altered YAP1 localization.
Wed Jun 27 00:00:00 EDT 2018
Bi-allelic Loss-of-Function Variants in DNMBP Cause Infantile Cataracts.
American journal of human genetics
Ansar M,Chung HL,Taylor RL,Nazir A,Imtiaz S,Sarwar MT,Manousopoulou A,Makrythanasis P,Saeed S,Falconnet E,Guipponi M,Pournaras CJ,Ansari MA,Ranza E,Santoni FA,Ahmed J,Shah I,Gul K,Black GC,Bellen HJ,Antonarakis SE
A-11122 was used in Immunohistochemistry-immunofluorescence to conclude that DNMBP loss-of-function variants cause infantile-onset cataracts in humans.
Thu Oct 04 00:00:00 EDT 2018
FUT2 Variants Confer Susceptibility to Familial Otitis Media.
American journal of human genetics
Santos-Cortez RLP,Chiong CM,Frank DN,Ryan AF,Giese APJ,Bootpetch Roberts T,Daly KA,Steritz MJ,Szeremeta W,Pedro M,Pine H,Yarza TKL,Scholes MA,Llanes EGDV,Yousaf S,Friedman N,Tantoco MLC,Wine TM,Labra PJ,Benoit J,Ruiz AG,de la Cruz RAR,Greenlee C,Yousaf A,Cardwell J,Nonato RMA,Ray D,Ong KMC,So E,Robertson CE,Dinwiddie J,Lagrana-Villagracia SM,Gubbels SP,Shaikh RS,Cass SP,Einarsdottir E,Lee NR,Schwartz DA,Gloria-Cruz TLI,Bamshad MJ,Yang IV,Kere J,Abes GT,Prager JD,Riazuddin S,Chan AL,Yoon PJ,Nickerson DA,Cutiongco-de la Paz EM,Streubel SO,Reyes-Quintos MRT,Jenkins HA,Mattila P,Chan KH,Mohlke KL,Leal SM,Hafrén L,Chonmaitree T,Sale MM,Ahmed ZM
A-11122 was used in Western Blotting to determine the role of FUT2 in otitis media susceptibility.
Thu Nov 01 00:00:00 EDT 2018
βIV Spectrinopathies Cause Profound Intellectual Disability, Congenital Hypotonia, and Motor Axonal Neuropathy.
American journal of human genetics
Wang CC,Ortiz-González XR,Yum SW,Gill SM,White A,Kelter E,Seaver LH,Lee S,Wiley G,Gaffney PM,Wierenga KJ,Rasband MN
A-11122 was used in Immunoprecipitation to define a class of spectrinopathies and reveal the molecular pathologies causing nervous-system dysfunction.
Thu Jun 07 00:00:00 EDT 2018
Circadian Waves of Transcriptional Repression Shape PIF-Regulated Photoperiod-Responsive Growth in Arabidopsis.
Current biology : CB
Martín G,Rovira A,Veciana N,Soy J,Toledo-Ortiz G,Gommers CMM,Boix M,Henriques R,Minguet EG,Alabadí D,Halliday KJ,Leivar P,Monte E
A-11122 was used in Immunoprecipitation to provide a framework for recent TIMING OF CAB EXPRESSION 1 (TOC1/PRR1) data [5, 20] and reveal that the long described circadian morning-to-midnight waves of the PRR transcriptional repressors (PRR9, PRR7, PRR5, and TOC1) [21] jointly gate PIF activity to dawn to prevent overgrowth through sequential regulation of common PIF-PRR target genes such as CDF5.
Mon Jan 22 00:00:00 EST 2018
The Bilateral Prefronto-striatal Pathway Is Necessary for Learning New Goal-Directed Actions.
Current biology : CB
Hart G,Bradfield LA,Fok SY,Chieng B,Balleine BW
A-11122 was used in Immunohistochemistry-immunofluorescence to demonstrate that the acquisition of new goal-directed actions depends on the bilateral PL-pDMS pathway driven by intratelencephalic cortical neurons.
Mon Jul 23 00:00:00 EDT 2018
Remodeling the Specificity of an Endosomal CORVET Tether Underlies Formation of Regulated Secretory Vesicles in the Ciliate Tetrahymena thermophila.
Current biology : CB
Sparvoli D,Richardson E,Osakada H,Lan X,Iwamoto M,Bowman GR,Kontur C,Bourland WA,Lynn DH,Pritchard JK,Haraguchi T,Dacks JB,Turkewitz AP
A-11122 was used in Immunocytochemistry-immunoflourescence to underscore that biogenesis of mucocysts depends on endolysosomal trafficking, revealing parallels with invasive organelles in apicomplexan parasites and suggesting that a wide array of secretory adaptations in protists, like in animals, depend on mechanisms related to lysosome biogenesis.
Mon Mar 05 00:00:00 EST 2018
A GABAergic Feedback Shapes Dopaminergic Input on the Drosophila Mushroom Body to Promote Appetitive Long-Term Memory.
Current biology : CB
Pavlowsky A,Schor J,Plaçais PY,Preat T
A-11122 was used in Immunohistochemistry to propose that this dual-receptor feedback supports a bidirectional self-regulation of MP1 input to the MB.
Mon Jun 04 00:00:00 EDT 2018
Endothelial Mitochondrial Preprotein Translocase Tomm7-Rac1 Signaling Axis Dominates Cerebrovascular Network Homeostasis.
Arteriosclerosis, thrombosis, and vascular biology
Shi D,Qi M,Zhou L,Li X,Ni L,Li C,Yuan T,Wang Y,Chen Y,Hu C,Liang D,Li L,Liu Y,Li J,Chen YH
A-11122 was used in Immunohistochemistry to study Tomm7 in driving brain angiogenesis and cerebrovascular network formation via mitochondrial signalling modulation.
Thu Nov 01 00:00:00 EDT 2018
Supplemental Treatment for Huntington's Disease with miR-132 that Is Deficient in Huntington's Disease Brain.
Molecular therapy. Nucleic acids
Fukuoka M,Takahashi M,Fujita H,Chiyo T,Popiel HA,Watanabe S,Furuya H,Murata M,Wada K,Okada T,Nagai Y,Hohjoh H
A-11122 was used in Immunohistochemistry-immunofluorescence to show amelioration in motor function and lifespan of Huntington's disease (HD)-model mice, R6/2 mice, by supplying miR-132 to HD brains using a recombinant adeno-associated virus (rAAV) miRNA expression system.
Fri Jun 01 00:00:00 EDT 2018
Rif1 prolongs the embryonic S phase at the Drosophila mid-blastula transition.
PLoS biology
Seller CA,O'Farrell PH
A-11122 was used in Immunohistochemistry to shows that Rif1 and S phase kinases compose a replication timer controlling first the developmental onset of late replication and then the precise schedule of replication within S phase.
Tue May 01 00:00:00 EDT 2018
Multiple origins and modularity in the spatiotemporal emergence of cerebellar astrocyte heterogeneity.
PLoS biology
Cerrato V,Parmigiani E,Figueres-Oñate M,Betizeau M,Aprato J,Nanavaty I,Berchialla P,Luzzati F,de'Sperati C,López-Mascaraque L,Buffo A
A-11122 was used in Immunohistochemistry-immunofluorescence to demonstrate that the major cerebellar astrocyte types emerge according to an unprecedented and remarkably orderly developmental program.
Sat Sep 01 00:00:00 EDT 2018
Hey1- and p53-dependent TrkC proapoptotic activity controls neuroblastoma growth.
PLoS biology
Ménard M,Costechareyre C,Ichim G,Blachier J,Neves D,Jarrosson-Wuilleme L,Depping R,Koster J,Saintigny P,Mehlen P,Tauszig-Delamasure S
A-11122 was used in Immunoprecipitation to demonstrate that Hey1 and TrkC-KF transcriptionally silence mouse double minute 2 homolog (MDM2), thus contributing to p53 stabilization.
Tue May 01 00:00:00 EDT 2018
Modulation of Cl- signaling and ion transport by recruitment of kinases and phosphatases mediated by the regulatory protein IRBIT.
Science signaling
Vachel L,Shcheynikov N,Yamazaki O,Fremder M,Ohana E,Son A,Shin DM,Yamazaki-Nakazawa A,Yang CR,Knepper MA,Muallem S
A-11122 was used in Immunoprecipitation to reveal how multiple kinase and phosphatase pathways use phosphorylation sites to fine-tune a transporter which has important implications for epithelial fluid and HCO3- secretion.
Tue Oct 30 00:00:00 EDT 2018
Analysis of the distribution of spinal NOP receptors in a chronic pain model using NOP-eGFP knock-in mice.
British journal of pharmacology
Ozawa A,Brunori G,Cippitelli A,Toll N,Schoch J,Kieffer BL,Toll L
A-11122 was used in Immunohistochemistry-immunofluorescence to investigate the peripheral and spinal NOP receptor distribution and antinociceptive effects of intrathecal nociceptin/orphanin FQ (N/OFQ) in chronic neuropathic pain.
Sun Jul 01 00:00:00 EDT 2018
RNA cytosine methyltransferase Nsun3 regulates embryonic stem cell differentiation by promoting mitochondrial activity.
Cellular and molecular life sciences : CMLS
Trixl L,Amort T,Wille A,Zinni M,Ebner S,Hechenberger C,Eichin F,Gabriel H,Schoberleitner I,Huang A,Piatti P,Nat R,Troppmair J,Lusser A
A-11122 was used in Immunocytochemistry-immunoflourescence to not only characterize Nsun3 as an important regulator of stem cell fate but also provide a model system to study the still incompletely understood interplay of mitochondrial function with stem cell pluripotency and differentiation.
Sun Apr 01 00:00:00 EDT 2018
Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.
EMBO reports
Shakya S,Sharma P,Bhatt AM,Jani RA,Delevoye C,Setty SR
A-11122 was used in Western Blotting to suggest that Rab22A promotes the assembly of a BLOC-1-BLOC-2-KIF13A complex on E/SEs to generate REs that maintain cellular and organelle homeostasis.
Sat Dec 01 00:00:00 EST 2018
Functional interplay between c-Myc and Max in B lymphocyte differentiation.
EMBO reports
Pérez-Olivares M,Trento A,Rodriguez-Acebes S,González-Acosta D,Fernández-Antorán D,Román-García S,Martinez D,López-Briones T,Torroja C,Carrasco YR,Méndez J,Moreno de Alborán I
A-11122 was used in Immunohistochemistry-immunofluorescence to suggest that c-Myc/Max heterodimers are not essential for the initiation of a subset of important biological processes in B lymphocytes, but are required for fine-tuning the initial response after activation.
Mon Oct 01 00:00:00 EDT 2018
Interaction of heterotrimeric kinesin-II with IFT-B-connecting tetramer is crucial for ciliogenesis.
The Journal of cell biology
Funabashi T,Katoh Y,Okazaki M,Sugawa M,Nakayama K
A-11122 was used in Western Blotting to determine the molecular basis of trafficking of intraflagellar transport particles regulated by kinesin and dynein motors.
Mon Aug 06 00:00:00 EDT 2018
Spatiotemporal organization of exocytosis emerges during neuronal shape change.
The Journal of cell biology
Urbina FL,Gomez SM,Gupton SL
A-11122 was used in Immunoprecipitation to exploit total internal reflection fluorescence microscopy to image vesicle-associated membrane protein (VAMP)-pHluorin-mediated exocytosis in mouse embryonic cortical neurons and interphase melanoma cells, and develop computer-vision software and statistical tools to uncover spatiotemporal aspects of exocytosis.
Mon Mar 05 00:00:00 EST 2018
Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers.
The Journal of cell biology
Ripoll L,Heiligenstein X,Hurbain I,Domingues L,Figon F,Petersen KJ,Dennis MK,Houdusse A,Marks MS,Raposo G,Delevoye C
A-11122 was used in Immunomicroscopy to study the mechanism underlying organelle function.
Mon Aug 06 00:00:00 EDT 2018
KIF1Bβ mutations detected in hereditary neuropathy impair IGF1R transport and axon growth.
The Journal of cell biology
Xu F,Takahashi H,Tanaka Y,Ichinose S,Niwa S,Wicklund MP,Hirokawa N
A-11122 was used in Immunoprecipitation to show that the receptor tyrosine kinase IGF1R is a new direct binding partner of KIF1Bβ and its binding and transport is specifically impaired by the Y1087C mutation of KIF1Bβ when detected in hereditary neuropathic patients.
Mon Oct 01 00:00:00 EDT 2018
KIF1Bβ mutations detected in hereditary neuropathy impair IGF1R transport and axon growth.
The Journal of cell biology
Xu F,Takahashi H,Tanaka Y,Ichinose S,Niwa S,Wicklund MP,Hirokawa N
A-11122 was used in Immunoprecipitation to show that the receptor tyrosine kinase IGF1R is a new direct binding partner of KIF1Bβ and its binding and transport is specifically impaired by the Y1087C mutation of KIF1Bβ when detected in hereditary neuropathic patients.
Mon Oct 01 00:00:00 EDT 2018
ANKRD11 associated with intellectual disability and autism regulates dendrite differentiation via the BDNF/TrkB signaling pathway.
Neurobiology of disease
Ka M,Kim WY
A-11122 was used in Immunohistochemistry to study the neurological role of ANKRD11 during brain development.
Thu Mar 01 00:00:00 EST 2018
Conserved and divergent functions of Pax6 underlie species-specific neurogenic patterns in the developing amniote brain.
Development (Cambridge, England)
Yamashita W,Takahashi M,Kikkawa T,Gotoh H,Osumi N,Ono K,Nomura T
A-11122 was used in Immunohistochemistry to suggest that spatiotemporal changes in Pax6-dependent regulatory programs contributed to species-specific neurogenic patterns in mammalian and avian lineages, which underlie the morphological divergence of the amniote pallial architectures.
Mon Apr 16 00:00:00 EDT 2018
The mir-279/996 cluster represses receptor tyrosine kinase signaling to determine cell fates in the Drosophila eye.
Development (Cambridge, England)
Duan H,de Navas LF,Hu F,Sun K,Mavromatakis YE,Viets K,Zhou C,Kavaler J,Johnston RJ,Tomlinson A,Lai EC
A-11122 was used in Immunohistochemistry-immunofluorescence to study photoreceptors in the Drosophila eye and report on the involvement of miR-279/996 for its control.
Mon Apr 09 00:00:00 EDT 2018
Conserved regulation of Nodal-mediated left-right patterning in zebrafish and mouse.
Development (Cambridge, England)
Montague TG,Gagnon JA,Schier AF
A-11122 was used in Western Blotting to demonstrate conservation between zebrafish and mouse mechanisms of left-right patterning.
Mon Dec 10 00:00:00 EST 2018
A non-canonical role for the proneural gene Neurog1 as a negative regulator of neocortical neurogenesis.
Development (Cambridge, England)
Han S,Dennis DJ,Balakrishnan A,Dixit R,Britz O,Zinyk D,Touahri Y,Olender T,Brand M,Guillemot F,Kurrasch D,Schuurmans C
A-11122 was used in Immunohistochemistry to study the interactions bwteen Neurog 1 and Neurog 2 in the control of neocortical neurogenesis.
Mon Oct 01 00:00:00 EDT 2018
BMP- and neuropilin 1-mediated motor axon navigation relies on spastin alternative translation.
Development (Cambridge, England)
Jardin N,Giudicelli F,Ten Martín D,Vitrac A,De Gois S,Allison R,Houart C,Reid E,Hazan J,Fassier C
A-11122 was used in Immunohistochemistry to determine the roles of BMP and neuropilin-1 in neuronal development and homeostasis.
Wed Sep 12 00:00:00 EDT 2018
Krox20 defines a subpopulation of cardiac neural crest cells contributing to arterial valves and bicuspid aortic valve.
Development (Cambridge, England)
Odelin G,Faure E,Coulpier F,Di Bonito M,Bajolle F,Studer M,Avierinos JF,Charnay P,Topilko P,Zaffran S
A-11122 was used in Immunohistochemistry to demonstrate a crucial role for Krox20 in arterial valve development.
Wed Jan 03 00:00:00 EST 2018
Eyeless/Pax6 initiates eye formation non-autonomously from the peripodial epithelium.
Development (Cambridge, England)
Baker LR,Weasner BM,Nagel A,Neuman SD,Bashirullah A,Kumar JP
A-11122 was used in Immunohistochemistry to reveal an unexpected mechanism for how Pax6 controls eye development in Drosophila.
Thu Aug 02 00:00:00 EDT 2018
Dynamics and Functional Role of Dopaminergic Neurons in the Ventral Tegmental Area during Itch Processing.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Yuan L,Liang TY,Deng J,Sun YG
A-11122 was used in Immunohistochemistry to reveal a potential target for suppressing excessive scratching responses in patients with chronic itch.
Wed Nov 14 00:00:00 EST 2018
Targeting of NF-κB to Dendritic Spines Is Required for Synaptic Signaling and Spine Development.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Dresselhaus EC,Boersma MCH,Meffert MK
A-11122 was used in Western Blotting to support spatial localization at synapses as a key variable mediating selective stimulus-response coupling.
Wed Apr 25 00:00:00 EDT 2018
Schaffer Collateral Inputs to CA1 Excitatory and Inhibitory Neurons Follow Different Connectivity Rules.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Kwon O,Feng L,Druckmann S,Kim J
A-11122 was used in Immunohistochemistry to better understand hippocampal networks by providing new evidence for differently structured connectivity at a fine scale in hippocampal excitatory and inhibitory neurons.
Wed May 30 00:00:00 EDT 2018
Brain Circuits Mediating Opposing Effects on Emotion and Pain.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Cai YQ,Wang W,Paulucci-Holthauzen A,Pan ZZ
A-11122 was used in Immunohistochemistry to investigate how the amygdala circuits regulate negative and positive emotions, such as depression and anxiety induced by chronic pain.
Wed Jul 11 00:00:00 EDT 2018
An Image-Based miRNA Screen Identifies miRNA-135s As Regulators of CNS Axon Growth and Regeneration by Targeting Krüppel-like Factor 4.
The Journal of neuroscience : the official journal of the Society for Neuroscience
van Battum EY,Verhagen MG,Vangoor VR,Fujita Y,Derijck AAHA,O'Duibhir E,Giuliani G,de Gunst T,Adolfs Y,Lelieveld D,Egan D,Schaapveld RQJ,Yamashita T,Pasterkamp RJ
A-11122 was used in Immunocytochemistry to identify a novel neuronal role for miR-135s and the miR-135-KLF4 pathway and highlight the potential of miRNAs as tools for enhancing CNS axon regeneration in mice.
Wed Jan 17 00:00:00 EST 2018
Genome Editing Reveals Idiosyncrasy of CNGA2 Ion Channel-Directed Antibody Immunoreactivity Toward Oxytocin.
Frontiers in cell and developmental biology
Blechman J,Anbalagan S,Matthews GG,Levkowitz G
A-11122 was used in Immunohistochemistry to determine the role of CNGA2 in oxytocin neurons function.
Thu Oct 01 00:00:00 EDT 2020
An Indispensable Role of Androgen Receptor in Wnt Responsive Cells During Prostate Development, Maturation, and Regeneration.
Stem cells (Dayton, Ohio)
He Y,Hooker E,Yu EJ,Wu H,Cunha GR,Sun Z
A-11122 was used in Immunocytochemistry-immunoflourescence to demonstrate an indispensable role for the Ar in Wnt-responsive cells during the course of prostate development, morphogenesis, and regeneration, which also imply an underlying interaction between the androgen and Wnt signaling pathways in the mouse prostate.
Fri Jun 01 00:00:00 EDT 2018
Notch Signaling Regulates Lgr5+ Olfactory Epithelium Progenitor/Stem Cell Turnover and Mediates Recovery of Lesioned Olfactory Epithelium in Mouse Model.
Stem cells (Dayton, Ohio)
Dai Q,Duan C,Ren W,Li F,Zheng Q,Wang L,Li W,Lu X,Ni W,Zhang Y,Chen Y,Wen T,Yu Y,Yu H
A-11122 was used in Immunocytochemistry-immunofluorescence to report that leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5) is expressed in globose basal cells (GBCs) localized in OE.
Wed Aug 01 00:00:00 EDT 2018
Hierarchical Control of Drosophila Sleep, Courtship, and Feeding Behaviors by Male-Specific P1 Neurons.
Neuroscience bulletin
Zhang W,Guo C,Chen D,Peng Q,Pan Y
A-11122 was used in Immunohistochemistry-immunofluorescence to investigate how a set of male-specific P1 neurons controls sleep, courtship, and feeding behaviors in Drosophila males.
Sat Dec 01 00:00:00 EST 2018
Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells.
Journal of cell science
Ghosh S,Scozzaro S,Ramos AR,Delcambre S,Chevalier C,Krejci P,Erneux C
A-11122 was used in Immunohistochemistry-immunofluorescence to determine the role of SHIP2 in cell migration in breast cancer cells.
Thu Aug 16 00:00:00 EDT 2018
LCP1 preferentially binds clasped αMβ2 integrin and attenuates leukocyte adhesion under flow.
Journal of cell science
Tseng HY,Samarelli AV,Kammerer P,Scholze S,Ziegler T,Immler R,Zent R,Sperandio M,Sanders CR,Fässler R,Böttcher RT
A-11122 was used in Flow cytometry/Cell sorting to offer a global view on cytoplasmic proteins interacting with different integrins and provide evidence for the existence of conformation-specific integrin interactors.
Wed Nov 21 00:00:00 EST 2018
Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription.
Journal of cell science
Rubio A,García-Blanco N,Vázquez-Bolado A,Belén Suárez M,Moreno S
A-11122 was used in Western Blotting to compare the phenotypes of cells lacking Rum1 and the anaphase-promoting complex/cyclosome (APC/C) activator Ste9 in nitrogen-rich and nitrogen-poor media.
Thu Sep 20 00:00:00 EDT 2018
Functional analyses of Pericentrin and Syne-2 interaction in ciliogenesis.
Journal of cell science
Falk N,Kessler K,Schramm SF,Boldt K,Becirovic E,Michalakis S,Regus-Leidig H,Noegel AA,Ueffing M,Thiel CT,Roepman R,Brandstätter JH,Gießl A
A-11122 was used in Immunocytochemistry to reveal the essential interaction between Pericentrin (Pcnt) and Syne-2 during ciliogenesis in photoreceptors of mouse retina.
Fri Aug 17 00:00:00 EDT 2018
Rab4A organizes endosomal domains for sorting cargo to lysosome-related organelles.
Journal of cell science
Nag S,Rani S,Mahanty S,Bissig C,Arora P,Azevedo C,Saiardi A,van der Sluijs P,Delevoye C,van Niel G,Raposo G,Setty SRG
A-11122 was used in Western Blotting to demonstrate Rab4A acts a key regulator of cargo segregation on SEs.
Thu Sep 20 00:00:00 EDT 2018
Proteasome stress leads to APP axonal transport defects by promoting its amyloidogenic processing in lysosomes.
Journal of cell science
Otero MG,Fernandez Bessone I,Hallberg AE,Cromberg LE,De Rossi MC,Saez TM,Levi V,Almenar-Queralt A,Falzone TL
A-11122 was used in Western Blotting to study the effect of proteasome activity on the regulation of axonal transport and metabolism on the amyloid precursor protein (APP).
Mon Jun 11 00:00:00 EDT 2018
Cholera toxin inhibits SNX27-retromer-mediated delivery of cargo proteins to the plasma membrane.
Journal of cell science
Singh V,Yang J,Yin J,Cole R,Tse M,Berman DE,Small SA,Petsko G,Donowitz M
A-11122 was used in Western Blotting to study the mechanisms by which cholera toxin inhibits NHE3.
Fri Aug 17 00:00:00 EDT 2018
Rab6 promotes insulin receptor and cathepsin trafficking to regulate autophagy induction and activity in Drosophila.
Journal of cell science
Ayala CI,Kim J,Neufeld TP
A-11122 was used in Immunohistochemistry-immunofluorescence to suggest that Rab6 acts to maintain the reciprocal regulation between autophagy and TOR activity during distinct nutrient states, thereby balancing autophagosome production and turnover to avoid autophagic stress.
Fri Sep 07 00:00:00 EDT 2018
Rab6 promotes insulin receptor and cathepsin trafficking to regulate autophagy induction and activity in Drosophila.
Journal of cell science
Ayala CI,Kim J,Neufeld TP
A-11122 was used in Immunohistochemistry-immunofluorescence to suggest that Rab6 acts to maintain the reciprocal regulation between autophagy and TOR activity during distinct nutrient states, thereby balancing autophagosome production and turnover to avoid autophagic stress.
Fri Sep 07 00:00:00 EDT 2018
Stress granule formation is induced by a threshold temperature rather than a temperature difference in Arabidopsis.
Journal of cell science
Hamada T,Yako M,Minegishi M,Sato M,Kamei Y,Yanagawa Y,Toyooka K,Watanabe Y,Hara-Nishimura I
A-11122 was used in Immunomicroscopy to provide new insights into the universal nature of stress granules in eukaryotic cells.
Mon Aug 20 00:00:00 EDT 2018
Region specific knock-out reveals distinct roles of chromatin modifiers in adult neurogenic niches.
Cell cycle (Georgetown, Tex.)
Rhodes CT,Zunino G,Huang SA,Cardona SM,Cardona AE,Berger MS,Lemmon VP,Lin CA
A-11122 was used in Flow cytometry/Cell sorting to identify the distinct roles of chromatin modifiers in adult neurogenic niches.
Fri Sep 06 00:00:00 EDT 2019
Self-assembly of FUS through its low-complexity domain contributes to neurodegeneration.
Human molecular genetics
Matsumoto T,Matsukawa K,Watanabe N,Kishino Y,Kunugi H,Ihara R,Wakabayashi T,Hashimoto T,Iwatsubo T
A-11122 was used in Western Blotting to study the molecular mechanism by which fused in sarcoma protein causes neurodegeneration.
Sun Apr 15 00:00:00 EDT 2018
The Cytotoxicity of Epsilon Toxin from Clostridium perfringens on Lymphocytes Is Mediated by MAL Protein Expression.
Molecular and cellular biology
Blanch M,Dorca-Arévalo J,Not A,Cases M,Gómez de Aranda I,Martínez-Yélamos A,Martínez-Yélamos S,Solsona C,Blasi J
A-11122 was used in Western Blotting to show that Etx selectively recognises and kills only human cell lines expressing myelin and lymphocyte protein through a direct Etx-MAL protein interaction.
Mon Oct 01 00:00:00 EDT 2018
Notch Signaling Regulates Differentiation and Steroidogenesis in Female Mouse Ovarian Granulosa Cells.
Endocrinology
Prasasya RD,Mayo KE
A-11122 was used in Immunohistochemistry to reveal a role of Notch signaling in promoting the differentiation of preovulatory granulosa cells.
Mon Jan 01 00:00:00 EST 2018
The Krüppel-Like Factor Gene Target Dusp14 Regulates Axon Growth and Regeneration.
Investigative ophthalmology & visual science
Galvao J,Iwao K,Apara A,Wang Y,Ashouri M,Shah TN,Blackmore M,Kunzevitzky NJ,Moore DL,Goldberg JL
A-11122 was used in Immunohistochemistry to reveal that Krüppel-Like Factor 9 (KLF9) drives the expression of dual specific phosphatase-14 (Dusp14) which regulates survival and axon regeneration after optic nerve injury.
Fri Jun 01 00:00:00 EDT 2018
A stochastic framework to model axon interactions within growing neuronal populations.
PLoS computational biology
Razetti A,Medioni C,Malandain G,Besse F,Descombes X
A-11122 was used in Immunohistochemistry-immunofluorescence to develop a stochastic model of 3D axon growth that takes into account spatial environmental constraints, physical interactions between neighbouring axons and branch formation.
Sat Dec 01 00:00:00 EST 2018
Role of mGlu2 in the 5-HT2A receptor-dependent antipsychotic activity of clozapine in mice.
Psychopharmacology
Hideshima KS,Hojati A,Saunders JM,On DM,de la Fuente Revenga M,Shin JM,Sánchez-González A,Dunn CM,Pais AB,Pais AC,Miles MF,Wolstenholme JT,González-Maeso J
A-11122 was used in Immunohistochemistry to test antipsychotic-like behavioural phenotypes induced by the atypical antipsychotic clozapine in mGlu2-KO mice and wild-type control littermates.
Thu Nov 01 00:00:00 EDT 2018
Mass Spectrometry-Based Screening Platform Reveals Orco Interactome in Drosophila melanogaster.
Molecules and cells
Yu KE,Kim DH,Kim YI,Jones WD,Lee JE
A-11122 was used in Western Blotting to characterise the Orco interactome from Drosophila melanogaster using mass spectrometry screening.
Wed Feb 28 00:00:00 EST 2018
The O-GlcNAc transferase OGT interacts with and post-translationally modifies the transcription factor HOXA1.
FEBS letters
Draime A,Bridoux L,Belpaire M,Pringels T,Degand H,Morsomme P,Rezsohazy R
A-11122 was used in Immunocytochemistry-immunoflourescence to reveal distinct partner proteins interacting with HOXA1.
Sun Apr 01 00:00:00 EDT 2018
Spatiotemporal dynamics of canonical Wnt signaling during embryonic eye development and posterior capsular opacification (PCO).
Experimental eye research
Wang Y,Mahesh P,Wang Y,Novo SG,Shihan MH,Hayward-Piatkovskyi B,Duncan MK
A-11122 was used in Immunohistochemistry to understand the distribution of canonical Wnt signalling during embryonic eye development and posterior capsular opacification.
Mon Oct 01 00:00:00 EDT 2018
The circadian gene Nr1d1 in the mouse nucleus accumbens modulates sociability and anxiety-related behaviour.
The European journal of neuroscience
Zhao C,Gammie SC
A-11122 was used in Immunohistochemistry-immunofluorescence to suggest that Nr1d1 in the NAc modulates sociability and anxiety-related behaviour in a sex-specific manner, and circadian, histone deacetylase and opioid-related genes may be involved in the expression of these behavioural phenotypes.
Wed Aug 01 00:00:00 EDT 2018
Rap1 GTPase promotes coordinated collective cell migration in vivo.
Molecular biology of the cell
Sawant K,Chen Y,Kotian N,Preuss KM,McDonald JA
A-11122 was used in Immunohistochemistry-immunofluorescence to propose that precisely regulated Rap1 activity reinforces connections between cells and polarizes the cluster, thus facilitating the coordinated collective migration of border cells.
Thu Nov 01 00:00:00 EDT 2018
Lipid biosensor interactions with wild type and matrix deletion HIV-1 Gag proteins.
Virology
Barklis E,Staubus AO,Mack A,Harper L,Barklis RL,Alfadhli A
A-11122 was used in Immunocytochemistry-immunoflourescence to demonstrate an approach to the analysis of viral protein-lipid associations and provide data on the lipid compositions of HIV-1 assembly sites.
Tue May 01 00:00:00 EDT 2018
Retinoic acid temporally orchestrates colonization of the gut by vagal neural crest cells.
Developmental biology
Uribe RA,Hong SS,Bronner ME
A-11122 was used in Immunohistochemistry-immunofluorescence to suggest that retinoic acid plays a critical temporal role in promoting enteric neural crest chain migration and neuronal survival upstream of Meis3 and RET in vivo.
Mon Jan 01 00:00:00 EST 2018
Maternal Nanos-Dependent RNA Stabilization in the Primordial Germ Cells of Drosophila Embryos.
Development, growth & differentiation
Sugimori S,Kumata Y,Kobayashi S
A-11122 was used in Immunohistochemistry-immunofluorescence to find that the level of mRNA expressed from a reporter gene fused to the CG32425 3' UTR was significantly reduced in PGCs lacking maternal Nos (nos PGCs) as compared with normal PGCs.
Mon Jan 01 00:00:00 EST 2018
Imaging pathological activities of human brain tissue in organotypic culture.
Journal of neuroscience methods
Le Duigou C,Savary E,Morin-Brureau M,Gomez-Dominguez D,Sobczyk A,Chali F,Milior G,Kraus L,Meier JC,Kullmann DM,Mathon B,de la Prida LM,Dorfmuller G,Pallud J,Eugène E,Clemenceau S,Miles R
A-11122 was used in Immunohistochemistry-immunofluorescence to show that organotypic culture techniques can be used to maintain brain tissue from patients with neurological syndromes for several weeks in vitro.
Thu Mar 15 00:00:00 EDT 2018
larvalign: Aligning Gene Expression Patterns from the Larval Brain of Drosophila melanogaster.
Neuroinformatics
Muenzing SEA,Strauch M,Truman JW,Bühler K,Thum AS,Merhof D
A-11122 was used in Immunohistochemistry-immunofluorescence to provide a manually annotated gold standard that serves for evaluation of the registration framework involved in template generation and mapping.
Mon Jan 01 00:00:00 EST 2018
In vivo analyses of radial spoke transport, assembly, repair and maintenance.
Cytoskeleton (Hoboken, N.J.)
Lechtreck KF,Mengoni I,Okivie B,Hilderhoff KB
A-11122 was used in Western Blotting to study radial spokes dynamics in vivo.
Wed Aug 01 00:00:00 EDT 2018
Identification of Novel Gata3 Distal Enhan
