|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:20-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||See 4 publications below|
|Immunoprecipitation (IP)||See 4 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 5 publications below|
|Western Blot (WB)||See 9 publications below|
|Immunohistochemistry - Free Floating (IHC (Free))||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|Immunohistochemistry (IHC)||See 15 publications below|
|Immunocytochemistry (ICC)||See 4 publications below|
|Immunofluorescence (IF)||See 14 publications below|
|Tested Species reactivity||Tag|
|Published species reactivity||Yeast , Rat , C. elegans , Fruit fly , Non-human primate , Cat , Zebrafish , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage conditions||-20° C, store in dark|
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Storage and reconstitution: to prepare a 0.2 mg/mL stock solution, reconstitute the lyophilized antibody in 0.5 mL PBS, pH 7.4. Reconstituted product can be stored for up to 3 months at 4°C with the addition of 2mM sodium azide.
The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organinsms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcelluclar compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used etensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: eGFP; GFP; GFP2; Green Fluoresecent Protein; Turbo GFP
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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