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|Tested species reactivity||Tag|
|Published species reactivity||Yeast, Rat, C. elegans, Fruit fly, Non-human primate, Cat, Zebrafish, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, store in dark|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:20-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Storage and reconstitution: to prepare a 0.2 mg/mL stock solution, reconstitute the lyophilized antibody in 0.5 mL PBS, pH 7.4. Reconstituted product can be stored for up to 3 months at 4°C with the addition of 2mM sodium azide.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Fruit fly||Not Cited||Hexameric GFP and mCherry reporters for the Drosophila GAL4, Q, and LexA transcription systems.||Shearin HK,Macdonald IS,Spector LP,Stowers RS||Genetics (196:951)||2014|
|Cat||Not Cited||ORF7-encoded accessory protein 7a of feline infectious peritonitis virus as a counteragent against IFN-α-induced antiviral response.||Dedeurwaerder A,Olyslaegers DA,Desmarets LM,Roukaerts ID,Theuns S,Nauwynck HJ||The Journal of general virology (95:393)||2014|
|Rat||1:200||Plasminogen activator inhibitor 1 RNA-binding protein interacts with progesterone receptor membrane component 1 to regulate progesterone's ability to maintain the viability of spontaneously immortalized granulosa cells and rat granulosa cells.||Peluso JJ,Yuan A,Liu X,Lodde V||Biology of reproduction (88:-)||2013|
|Rat||Not Cited||The IE180 protein of pseudorabies virus suppresses phosphorylation of translation initiation factor eIF2α.||Van Opdenbosch N,Van den Broeke C,De Regge N,Tabarés E,Favoreel HW||Journal of virology (86:7235)||2012|
|Mouse||1:5000||BID-F1 and BID-F2 domains of Bartonella henselae effector protein BepF trigger together with BepC the formation of invasome structures.||Truttmann MC,Guye P,Dehio C||PloS one (6:null)||2011|
|Human||Not Cited||RasGRF suppresses Cdc42-mediated tumour cell movement, cytoskeletal dynamics and transformation.||Calvo F,Sanz-Moreno V,Agudo-Ibáñez L,Wallberg F,Sahai E,Marshall CJ,Crespo P||Nature cell biology (13:819)||2011|
AAV9-mediated expression of a non-self protein in nonhuman primate central nervous system triggers widespread neuroinflammation driven by antigen-presenting cell transduction.
G10362 was used in immunohistochemistry to study the neurotoxicity of AAV9-mediated expression of a non-self protein in nonhuman primates
|Samaranch L,San Sebastian W,Kells AP,Salegio EA,Heller G,Bringas JR,Pivirotto P,DeArmond S,Forsayeth J,Bankiewicz KS||Molecular therapy : the journal of the American Society of Gene Therapy (22:329)||2014|
|Urinary bladder matrix promotes site appropriate tissue formation following right ventricle outflow tract repair.||Remlinger NT,Gilbert TW,Yoshida M,Guest BN,Hashizume R,Weaver ML,Wagner WR,Brown BN,Tobita K,Wearden PD||Organogenesis (9:149)||2013|
|Mouse||Not Cited||The Munc13 proteins differentially regulate readily releasable pool dynamics and calcium-dependent recovery at a central synapse.||Chen Z,Cooper B,Kalla S,Varoqueaux F,Young SM||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:8336)||2013|
|Non-human primate||Not Cited||
Strong cortical and spinal cord transduction after AAV7 and AAV9 delivery into the cerebrospinal fluid of nonhuman primates.
G10362 was used in immunohistochemistry to evaluate the cellular transduction efficacy of intrathecal delivery of adeno-associated viruses
|Samaranch L,Salegio EA,San Sebastian W,Kells AP,Bringas JR,Forsayeth J,Bankiewicz KS||Human gene therapy (24:526)||2013|
|Rat||1:100||Minocycline-preconditioned neural stem cells enhance neuroprotection after ischemic stroke in rats.||Chan PH||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:3462)||2012|
|ColQ controls postsynaptic differentiation at the neuromuscular junction.||Sigoillot SM,Bourgeois F,Lambergeon M,Strochlic L,Legay C||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:13)||2010|
|Fruit fly||Not Cited||
The Drosophila platelet-derived growth factor and vascular endothelial growth factor-receptor related (Pvr) protein ligands Pvf2 and Pvf3 control hemocyte viability and invasive migration.
G10362 was used in immunohistochemistry to determine Pvf2 and Pvf3 functions in vivo using fruit flies.
|Parsons B,Foley E||The Journal of biological chemistry (288:20173)||2013|
|Fruit fly||1:200||An efficient method for recombineering GAL4 and QF drivers.||Stowers RS||Fly (5:371)||2013|
||Plasminogen activator inhibitor 1 RNA-binding protein interacts with progesterone receptor membrane component 1 to regulate progesterone's ability to maintain the viability of spontaneously immortalized granulosa cells and rat granulosa cells.||Peluso JJ,Yuan A,Liu X,Lodde V||Biology of reproduction (88:-)||2013|
|Mouse||Not Cited||Reduction of choroidal neovascularization in mice by adeno-associated virus-delivered anti-vascular endothelial growth factor short hairpin RNA.||Askou AL,Pournaras JA,Pihlmann M,Svalgaard JD,Arsenijevic Y,Kostic C,Bek T,Dagnaes-Hansen F,Mikkelsen JG,Jensen TG,Corydon TJ||The journal of gene medicine (14:632)||2012|
|Yeast||Not Cited||Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity.||Love KR,Politano TJ,Panagiotou V,Jiang B,Stadheim TA,Love JC||PloS one (7:null)||2012|
||Minocycline-preconditioned neural stem cells enhance neuroprotection after ischemic stroke in rats.||Chan PH||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:3462)||2012|
Identification of modulators of hair cell regeneration in the zebrafish lateral line.
G10362 was used in immunohistochemistry to investigate compounds that modulate hair cell regeneration in zebrafish.
|Namdaran P,Reinhart KE,Owens KN,Raible DW,Rubel EW||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:3516)||2012|
|C. elegans||Not Cited||UNC-33 (CRMP) and ankyrin organize microtubules and localize kinesin to polarize axon-dendrite sorting.||Maniar TA,Kaplan M,Wang GJ,Shen K,Wei L,Shaw JE,Koushika SP,Bargmann CI||Nature neuroscience (15:48)||2012|
|Fruit fly||Not Cited||Debra, a protein mediating lysosomal degradation, is required for long-term memory in Drosophila.||Kottler B,Lampin-Saint-Amaux A,Comas D,Preat T,Goguel V||PloS one (6:null)||2011|
|Fruit fly||1:200||A Gateway MultiSite recombination cloning toolkit.||Petersen LK,Stowers RS||PloS one (6:null)||2011|
|Mouse||Not Cited||An absolute requirement for Pax7-positive satellite cells in acute injury-induced skeletal muscle regeneration.||Lepper C,Partridge TA,Fan CM||Development (Cambridge, England) (138:3639)||2011|
|Rat||1:100||Inner limiting membrane barriers to AAV-mediated retinal transduction from the vitreous.||Dalkara D,Kolstad KD,Caporale N,Visel M,Klimczak RR,Schaffer DV,Flannery JG||Molecular therapy : the journal of the American Society of Gene Therapy (17:2096)||2009|
|Fruit fly||Not Cited||Cell type-specific genomics of Drosophila neurons.||Henry GL,Davis FP,Picard S,Eddy SR||Nucleic acids research (40:9691)||2012|
||RasGRF suppresses Cdc42-mediated tumour cell movement, cytoskeletal dynamics and transformation.||Calvo F,Sanz-Moreno V,Agudo-Ibáñez L,Wallberg F,Sahai E,Marshall CJ,Crespo P||Nature cell biology (13:819)||2011|