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|Tested species reactivity||Tag|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The GFP was isolated directly from the jellyfish Aequorea victoria.|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 ug/ml|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 5 publications below|
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
In vitro alterations do not reflect a requirement for host cell cycle progression during Plasmodium liver stage infection.
A-21311 was used in immunohistochemistry to examine the mechanism of parasite proliferation within plasmodium liver stage infection
|Hanson KK,March S,Ng S,Bhatia SN,Mota MM||Eukaryotic cell (14:96)||2015|
A general principle governs vision-dependent dendritic patterning of retinal ganglion cells.
A-21311 was used in immunohistochemistry to study the regulatory process for the dendritic patterning of retinal ganglion cells.
|Xu HP,Sun JH,Tian N||The Journal of comparative neurology (522:3403)||2014|
Colocalization of hyperpolarization-activated, cyclic nucleotide-gated channel subunits in rat retinal ganglion cells.
A-21311 was used in immunohistochemistry to investigate cyclic nucleotide-gated channel subunits in rat retinal ganglion cells.
|Stradleigh TW,Ogata G,Partida GJ,Oi H,Greenberg KP,Krempely KS,Ishida AT||The Journal of comparative neurology (519:2546)||2011|
Glycine receptor-mediated synaptic transmission regulates the maturation of ganglion cell synaptic connectivity.
A-21311 was used in immunohistochemistry to investigate the role of glycine receptor-mediated synaptic transmission in the maturation of ganglion cell synaptic connectivity.
|Xu HP,Tian N||The Journal of comparative neurology (509:53)||2008|
Postnatal cellular contributions of the hippocampus subventricular zone to the dentate gyrus, corpus callosum, fimbria, and cerebral cortex.
A-21311 was used in immunohistochemistry to study the role of hippocampus subventricular zone progenitors in the development of dentate gyrus, corpus callosum, fimbria, and cerebral cortex
|Navarro-Quiroga I,Hernandez-Valdes M,Lin SL,Naegele JR||The Journal of comparative neurology (497:833)||2006|