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|Tested species reactivity||Tag|
|Published species reactivity||Fruit fly, Human, Mouse, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The GFP was isolated directly from the jellyfish Aequorea victoria.|
|Storage buffer||whole serum|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Characterization of the octopaminergic and tyraminergic neurons in the central brain of Drosophila larvae.
A-6455 was used in immunohistochemistry to characterize the octopaminergic and tyraminergic neurons in Drosophila larvae brain.
|Selcho M,Pauls D,Huser A,Stocker RF,Thum AS||The Journal of comparative neurology (522:3485)||2014|
Lulu regulates Shroom-induced apical constriction during neural tube closure.
A-6455 was used in immunohistochemistry to study apical constriction dependent on Shroom3 and EPB4.1l5 within neural tube closure.
|Chu CW,Gerstenzang E,Ossipova O,Sokol SY||PloS one (8:null)||2013|
Transplantation reveals regional differences in oligodendrocyte differentiation in the adult brain.
A-6455 was used in immunohistochemistry to investigate oligodendrocyte differentiation in the adult brain.
|Viganò F,Möbius W,Götz M,Dimou L||Nature neuroscience (16:1370)||2013|
Spatiotemporal fate map of neurogenin1 (Neurog1) lineages in the mouse central nervous system.
A-6455 was used in immunohistochemistry to investigate the migration and maturation of neurogenin1 neurons in the rodent central nervous system.
|Kim EJ,Hori K,Wyckoff A,Dickel LK,Koundakjian EJ,Goodrich LV,Johnson JE||The Journal of comparative neurology (519:1355)||2011|
Identification of novel spinal cholinergic genetic subtypes disclose Chodl and Pitx2 as markers for fast motor neurons and partition cells.
A-6455 was used in immunohistochemistry to identify markers for fast motor neurons and partition cells.
|Enjin A,Rabe N,Nakanishi ST,Vallstedt A,Gezelius H,Memic F,Lind M,Hjalt T,Tourtellotte WG,Bruder C,Eichele G,Whelan PJ,Kullander K||The Journal of comparative neurology (518:2284)||2010|
Melanocortin-4 receptor expression in a vago-vagal circuitry involved in postprandial functions.
A-6455 was used in immunohistochemistry to study the expression and funtion of melanocortin-4 receptor in a vago-vagal circuitry.
|Gautron L,Lee C,Funahashi H,Friedman J,Lee S,Elmquist J||The Journal of comparative neurology (518:6)||2010|
Fate of endogenous stem/progenitor cells following spinal cord injury.
A-6455 was used in immunohistochemistry to investigate endogenous stem/progenitor cells after spinal cord injury.
|Horky LL,Galimi F,Gage FH,Horner PJ||The Journal of comparative neurology (498:525)||2006|
Expression of the vesicular glutamate transporter vGluT2 in a subset of cones of the mouse retina.
A-6455 was used in immunohistochemistry to localize vesicular glutamate transporter vGluT2 in a subset of cones of the mouse retina.
|Wässle H,Regus-Leidig H,Haverkamp S||The Journal of comparative neurology (496:544)||2006|
Down-modulation of nucleoporin RanBP2/Nup358 impaired chromosomal alignment and induced mitotic catastrophe.
A-6455 was used in immunocytochemistry, immunoprecipitation, and western blot to study the role of nucleoporin RanBP2/Nup358 in chromosomal alignment and mitotic integrity.
|Hashizume C,Kobayashi A,Wong RW||Cell death & disease (4:null)||2013|