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|Tested species reactivity||Tag|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Chicken / IgY|
|Immunogen||The GFP was isolated directly from the jellyfish Aequorea victoria.|
|Storage buffer||PBS, pH 7.2|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Differentiation potential of individual olfactory c-Kit+ progenitors determined via multicolor lineage tracing.
A10262 was used in immunohistochemistry - frozen section to assess the differentiation potential of individual globose cells
|Goss GM,Chaudhari N,Hare JM,Nwojo R,Seidler B,Saur D,Goldstein BJ||Developmental neurobiology (76:241)||2016|
T-box transcription regulator Tbr2 is essential for the formation and maintenance of Opn4/melanopsin-expressing intrinsically photosensitive retinal ganglion cells.
A10262 was used in immunohistochemistry - frozen section to investigate if Tbr2 regulates ipRGC formation and survival in vivo
|Mao CA,Li H,Zhang Z,Kiyama T,Panda S,Hattar S,Ribelayga CP,Mills SL,Wang SW||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:13083)||2014|
Olfactory ensheathing cells, but not Schwann cells, proliferate and migrate extensively within moderately X-irradiated juvenile rat brain.
A10262 was used in immunohistochemistry - frozen section to study migration of transplanted olfactory ensheathing cells and Schwann cells in rat spinal cord
|Lankford KL,Brown RJ,Sasaki M,Kocsis JD||Glia (62:52)||2014|
mTORC1 and mTORC2 have largely distinct functions in Purkinje cells.
A10262 was used in immunohistochemistry to understand the functions of mTORC1 and mTORC2 in Purkinje cells
|Angliker N,Burri M,Zaichuk M,Fritschy JM,Rüegg MA||The European journal of neuroscience (42:2595)||2015|
Biliary Epithelial Cells Are Not the Predominant Source of Hepatic CXCL12.
A10262 was used in immunohistochemistry to show that biliary epithelial cells are not the primary source of CXLC12
|Saiman Y,Sugiyama T,Simchoni N,Spirli C,Bansal MB||The American journal of pathology (185:1859)||2015|
A structural and genotypic scaffold underlying temporal integration.
A10262 was used in immunohistochemistry to map velocity-to-position neural integrator activity in zebrafish
|Lee MM,Arrenberg AB,Aksay ER||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:7903)||2015|
Imaging activity in astrocytes and neurons with genetically encoded calcium indicators following in utero electroporation.
A10262 was used in immunohistochemistry to describe an in utero electroporation method to add genes to study neurons in rats
|Gee JM,Gibbons MB,Taheri M,Palumbos S,Morris SC,Smeal RM,Flynn KF,Economo MN,Cizek CG,Capecchi MR,Tvrdik P,Wilcox KS,White JA||Frontiers in molecular neuroscience (8:null)||2015|
Structural basis for serotonergic regulation of neural circuits in the mouse olfactory bulb.
A10262 was used in immunohistochemistry to identify the mechanism for serotonergic regulation of neural circuits in the mouse olfactory bulb
|Suzuki Y,Kiyokage E,Sohn J,Hioki H,Toida K||The Journal of comparative neurology (523:262)||2015|
Differential responsiveness of distinct retinal domains to Atoh7.
A10262 was used in immunohistochemistry to explore the differential susceptibilities of early retinal progenitor cells to Atoh7 in vivo
|Sinn R,Peravali R,Heermann S,Wittbrodt J||Mechanisms of development (133:218)||2014|
TrkB signaling directs the incorporation of newly generated periglomerular cells in the adult olfactory bulb.
A10262 was used in immunohistochemistry to understand the role of TrkB in the integration of new periglomerular cells in the adult olfactory bulb
|Bergami M,Vignoli B,Motori E,Pifferi S,Zuccaro E,Menini A,Canossa M||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:11464)||2013|
Localization of neurones expressing the gap junction protein Connexin45 within the adult spinal dorsal horn: a study using Cx45-eGFP reporter mice.
A10262 was used in immunohistochemistry to identify Cx45-positive neurones in the adult spinal dorsal horn
|Chapman RJ,Lall VK,Maxeiner S,Willecke K,Deuchars J,King AE||Brain structure & function (218:751)||2013|
|Not Applicable||Not Cited||
Functional Effect of Pim1 Depends upon Intracellular Localization in Human Cardiac Progenitor Cells.
A10262 was used in western blot to show that subcellular targeting bolsters the cardioprotective effects of Pim1 in primary human cardiac progenitor cells
|Samse K,Emathinger J,Hariharan N,Quijada P,Ilves K,Völkers M,Ormachea L,De La Torre A,Orogo AM,Alvarez R,Din S,Mohsin S,Monsanto M,Fischer KM,Dembitsky WP,Gustafsson ÅB,Sussman MA||The Journal of biological chemistry (290:13935)||2015|
Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice.
A10262 was used in western blot to study adenoviral vectors that target tumor vascular endothelial cells.
|Lu ZH,Kaliberov S,Sohn RE,Kaliberova L,Curiel DT,Arbeit JM||PloS one (8:null)||2013|
Protein arginine methyltransferase 1 and 8 interact with FUS to modify its sub-cellular distribution and toxicity in vitro and in vivo.
A10262 was used in western blot to examine the role of arginine methylation within the pathogenesis of FUS-related amyotrophic lateral sclerosis
|Scaramuzzino C,Monaghan J,Milioto C,Lanson NA,Maltare A,Aggarwal T,Casci I,Fackelmayer FO,Pennuto M,Pandey UB||PloS one (8:null)||2013|
|Not Applicable||Not Cited||
Reduced ciliary polycystin-2 in induced pluripotent stem cells from polycystic kidney disease patients with PKD1 mutations.
A10262 was used in immunocytochemistry to investigate the relationship between pathopolycystin- and polycystin-2 in the pathogenesis of PKD.
|Freedman BS,Lam AQ,Sundsbak JL,Iatrino R,Su X,Koon SJ,Wu M,Daheron L,Harris PC,Zhou J,Bonventre JV||Journal of the American Society of Nephrology : JASN (24:1571)||2013|
CDKL5 ensures excitatory synapse stability by reinforcing NGL-1-PSD95 interaction in the postsynaptic compartment and is impaired in patient iPSC-derived neurons.
A10262 was used in immunocytochemistry to elucidate the function of CDKL5 in neurons.
|Ricciardi S,Ungaro F,Hambrock M,Rademacher N,Stefanelli G,Brambilla D,Sessa A,Magagnotti C,Bachi A,Giarda E,Verpelli C,Kilstrup-Nielsen C,Sala C,Kalscheuer VM,Broccoli V||Nature cell biology (14:911)||2012|
Maturation of spinal motor neurons derived from human embryonic stem cells.
A10262 was used in immunocytochemistry to examine the morphological and electrophysiological changes during the maturation of neurons differentiated from human embryonic stem.
|Takazawa T,Croft GF,Amoroso MW,Studer L,Wichterle H,Macdermott AB||PloS one (7:null)||2012|
Wnt signaling requires sequestration of glycogen synthase kinase 3 inside multivesicular endosomes.
A10262 was used in immunocytochemistry to determine the molecular mechanisms by which canonical Wnt signaling suppresses glycogen synthase kinase 3 activity.
|Taelman VF,Dobrowolski R,Plouhinec JL,Fuentealba LC,Vorwald PP,Gumper I,Sabatini DD,De Robertis EM||Cell (143:1136)||2010|
Cross talk between microRNA and epigenetic regulation in adult neurogenesis.
A10262 was used in immunocytochemistry to investigate the cross talk between miR-137 and epigenetic regulation in adult neural stem cells.
|Szulwach KE,Li X,Smrt RD,Li Y,Luo Y,Lin L,Santistevan NJ,Li W,Zhao X,Jin P||The Journal of cell biology (189:127)||2010|
Green Fluorescence Protein