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Catalog#: A10262 Invitrogen

Catalog #	Size	Price (USD)	Qty	Datasheet
A10262	100 µL	332.00

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Suggested Secondary Antibodies

Anti-Chicken Secondary Antibodies (61)
Alexa Fluor® Anti-Chicken Secondary Antibodies (7)
HRP Anti-Chicken Secondary Antibodies (10)

Product Details

References

Bioinformatics

Documents

Details
Tested species reactivity	Tag
Published species reactivity	Mouse, Not Applicable
Host / Isotype	Chicken / IgY
Class	Polyclonal
Type	Antibody
Immunogen	The GFP was isolated directly from the jellyfish Aequorea victoria.
Conjugate	Unconjugated
Form	Liquid
Purification	purified
Storage buffer	PBS, pH 7.2
Contains	5mM sodium azide
Storage Conditions	4° C

Tested Applications	Dilution ^*
Immunocytochemistry (ICC)	Assay Dependent
Immunofluorescence (IF)	Assay Dependent
Western Blot (WB)	Assay Dependent

^* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

Published Applications
Immunohistochemistry (Frozen) (IHC (F))	See 11 publications below
Immunohistochemistry (IHC)	See 25 publications below
Immunohistochemistry - Free Floating (IHC (Free))	See 2 publications below
Immunocytochemistry (ICC)	See 10 publications below
Immunohistochemistry (Paraffin) (IHC (P))	See 6 publications below
Miscellaneous PubMed (MISC)	See 8 publications below
Western Blot (WB)	See 4 publications below

Background/Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References

Immunohistochemistry (Frozen)

Species	Dilution	Title	Authors	Journal (Vol:Pg)	Year
Not Applicable	1:1000	Anatomical and molecular characterization of dopamine D1 receptor-expressing neurons of the mouse CA1 dorsal hippocampus. A10262 was used in immunohistochemistry - frozen section to examine D1 receptor-containing neurons within the CA1 subfield of the dorsal hippocampus	Puighermanal E,Cutando L,Boubaker-Vitre J,Honoré E,Longueville S,Hervé D,Valjent E	Brain structure and function (222:1897)	2017
Not Applicable	1:1000	Growth hormone biases amygdala network activation after fear learning. A10262 was used in immunohistochemistry - frozen section to study how growth hormone affects fear memory formation	Gisabella B,Farah S,Peng X,Burgos-Robles A,Lim SH,Goosens KA	Translational psychiatry (6:null)	2016
Not Applicable	1:500	Native Piezo2 Interactomics Identifies Pericentrin as a Novel Regulator of Piezo2 in Somatosensory Neurons. A10262 was used in immunohistochemistry - frozen section to identify pericentrin as a novel regulator of piezo2 in somatosensory neurons by native piezo2 interactomics	Narayanan P,Sondermann J,Rouwette T,Karaca S,Urlaub H,Mitkovski M,Gomez-Varela D,Schmidt M	Journal of proteome research (15:2676)	2016
Not Applicable	1:1000	Angiotensin Type-2 Receptors Influence the Activity of Vasopressin Neurons in the Paraventricular Nucleus of the Hypothalamus in Male Mice. A10262 was used in immunohistochemistry - frozen section to determine how influence is made regarding activity of vasopressin neurons in the paraventricular nucleus of the hypothalamus in male mice by angiotensin type-2 receptors	de Kloet AD,Pitra S,Wang L,Hiller H,Pioquinto DJ,Smith JA,Sumners C,Stern JE,Krause EG	Endocrinology (157:3167)	2016
Not Applicable	1:500	Differentiation potential of individual olfactory c-Kit+ progenitors determined via multicolor lineage tracing. A10262 was used in immunohistochemistry - frozen section to assess the differentiation potential of individual globose cells	Goss GM,Chaudhari N,Hare JM,Nwojo R,Seidler B,Saur D,Goldstein BJ	Developmental neurobiology (76:241)	2016
Not Applicable	1:800	Unsupervised lineage-based characterization of primate precursors reveals high proliferative and morphological diversity in the OSVZ. A10262 was used in immunohistochemistry - frozen section to develop a method of unbiased lineage analysis that automatically identifies cell types	Pfeiffer M,Betizeau M,Waltispurger J,Pfister SS,Douglas RJ,Kennedy H,Dehay C	The Journal of comparative neurology (524:535)	2016
Not Applicable	1:300	Protein-Trap Insertional Mutagenesis Uncovers New Genes Involved in Zebrafish Skin Development, Including a Neuregulin 2a-Based ErbB Signaling Pathway Required during Median Fin Fold Morphogenesis. A10262 was used in immunohistochemistry - frozen section to describe the Zebrafish Integument Project	Westcot SE,Hatzold J,Urban MD,Richetti SK,Skuster KJ,Harm RM,Lopez Cervera R,Umemoto N,McNulty MS,Clark KJ,Hammerschmidt M,Ekker SC	PloS one (10:null)	2015
Not Applicable	1:600	T-box transcription regulator Tbr2 is essential for the formation and maintenance of Opn4/melanopsin-expressing intrinsically photosensitive retinal ganglion cells. A10262 was used in immunohistochemistry - frozen section to investigate if Tbr2 regulates ipRGC formation and survival in vivo	Mao CA,Li H,Zhang Z,Kiyama T,Panda S,Hattar S,Ribelayga CP,Mills SL,Wang SW	The Journal of neuroscience : the official journal of the Society for Neuroscience (34:13083)	2014
Not Applicable	1:1000	The structure and organization of lanceolate mechanosensory complexes at mouse hair follicles. A10262 was used in immunohistochemistry - frozen section to study mouse hair follicles and the organization and structure of lanceolate mechanosensory complexes	Li L,Ginty DD	eLife (3:null)	2014
Not Applicable	1:1000	Olfactory ensheathing cells, but not Schwann cells, proliferate and migrate extensively within moderately X-irradiated juvenile rat brain. A10262 was used in immunohistochemistry - frozen section to study migration of transplanted olfactory ensheathing cells and Schwann cells in rat spinal cord	Lankford KL,Brown RJ,Sasaki M,Kocsis JD	Glia (62:52)	2014
Not Applicable	Not Cited	Directed differentiation of hippocampal stem/progenitor cells in the adult brain. A10262 was used in immunocytochemistry and immunohistochemistry - frozen section to analyze hippocampal stem/progenitor cells in the adult brain and directed differentiation	Jessberger S,Toni N,Clemenson GD,Ray J,Gage FH	Nature neuroscience (11:888)	2008

Immunohistochemistry

Species	Dilution	Title	Authors	Journal (Vol:Pg)	Year
Not Applicable	1:10,000	Structural basis for cholinergic regulation of neural circuits in the mouse olfactory bulb. A10262 was used in immunohistochemistry to investigate cholinergic regulation of the olfactory bulb and examine neural morphology	Hamamoto M,Kiyokage E,Sohn J,Hioki H,Harada T,Toida K	The Journal of comparative neurology (525:574)	2017
Not Applicable	1:500	Recombinant Adeno-Associated Virus Serotype 6 (rAAV6) Potently and Preferentially Transduces Rat Astrocytes In vitro and In vivo. A10262 was used in immunohistochemistry to evaluate commercially available adeno-associated virus serotypes for their ability to transduce primary rat astrocytes	Schober AL,Gagarkin DA,Chen Y,Gao G,Jacobson L,Mongin AA	Frontiers in cellular neuroscience (10:null)	2016
Not Applicable	1:1000	Glial Cell Calcium Signaling Mediates Capillary Regulation of Blood Flow in the Retina. A10262 was used in immunohistochemistry to report that glial calcium signaling modulates capillary but not arteriole blood flow	Biesecker KR,Srienc AI,Shimoda AM,Agarwal A,Bergles DE,Kofuji P,Newman EA	The Journal of neuroscience : the official journal of the Society for Neuroscience (36:9435)	2016
Not Applicable	1:50	Functional integration of a serotonergic neuron in the Drosophila antennal lobe. A10262 was used in immunohistochemistry clarify how serotonergic neurons respond to odor stimulation and integrate synaptically into local circuits	Zhang X,Gaudry Q	eLife (5:null)	2016
Not Applicable	1:100	Re-epithelialization of cutaneous wounds in adult zebrafish combines mechanisms of wound closure in embryonic and adult mammals. A10262 was used in immunohistochemistry to analyze the mechanism of wound closure in embryonic and adult mammals due to re-epithelialization of cutaneous wounds in adult zebrafish	Richardson R,Metzger M,Knyphausen P,Ramezani T,Slanchev K,Kraus C,Schmelzer E,Hammerschmidt M	Development (Cambridge, England) (143:2077)	2016
Not Applicable	1:500	Awake dynamics and brain-wide direct inputs of hypothalamic MCH and orexin networks. A10262 was used in immunohistochemistry to study orexin networks and hypothalamici MCH for awakek dynamics and brain-wide direct inputs	González JA,Iordanidou P,Strom M,Adamantidis A,Burdakov D	Nature communications (7:null)	2016
Not Applicable	1:500	Lineage mapping identifies molecular and architectural similarities between the larval and adult Drosophila central nervous system. A10262 was used in immunohistochemistry to study molecular and architectural differences by lineage m apping between larval and adult drosophila central nervous system	Lacin H,Truman JW	eLife (5:null)	2016
Not Applicable	1:1000	Sex-specific regulation of Lgr3 in Drosophila neurons. A10262 was used in immunohistochemistry to analyze sex-specific regulation of Lgr3 in Drosophila neurons	Meissner GW,Luo SD,Dias BG,Texada MJ,Baker BS	Proceedings of the National Academy of Sciences of the United States of America (113:E1256)	2016
Not Applicable	1:2000	GGGGCC microsatellite RNA is neuritically localized, induces branching defects, and perturbs transport granule function. A10262 was used in immunohistochemistry to characterize GGGGCC microsatelline RNA and induction of branching defects, neuritical localization, and perturbing of transport granule function	Burguete AS,Almeida S,Gao FB,Kalb R,Akins MR,Bonini NM	eLife (4:null)	2015
Not Applicable	1:500	mTORC1 and mTORC2 have largely distinct functions in Purkinje cells. A10262 was used in immunohistochemistry to understand the functions of mTORC1 and mTORC2 in Purkinje cells	Angliker N,Burri M,Zaichuk M,Fritschy JM,Rüegg MA	The European journal of neuroscience (42:2595)	2015
Not Applicable	1:500	The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. A10262 was used in immunohistochemistry to analyze protein kinase LRRK2, parkinson's disease associated, which modulates notch signaling via the endosomal pathway	Imai Y,Kobayashi Y,Inoshita T,Meng H,Arano T,Uemura K,Asano T,Yoshimi K,Zhang CL,Matsumoto G,Ohtsuka T,Kageyama R,Kiyonari H,Shioi G,Nukina N,Hattori N,Takahashi R	PLoS genetics (11:null)	2015
Not Applicable	1:200	Biliary Epithelial Cells Are Not the Predominant Source of Hepatic CXCL12. A10262 was used in immunohistochemistry to show that biliary epithelial cells are not the primary source of CXLC12	Saiman Y,Sugiyama T,Simchoni N,Spirli C,Bansal MB	The American journal of pathology (185:1859)	2015
Not Applicable	1:1000	A neural basis for melanocortin-4 receptor-regulated appetite. A10262 was used in immunohistochemistry to show the bidirectional control of feeding following real-time activation and inhibition of PVH(MC4R) neurons	Garfield AS,Li C,Madara JC,Shah BP,Webber E,Steger JS,Campbell JN,Gavrilova O,Lee CE,Olson DP,Elmquist JK,Tannous BA,Krashes MJ,Lowell BB	Nature neuroscience (18:863)	2015
Not Applicable	1:400	A structural and genotypic scaffold underlying temporal integration. A10262 was used in immunohistochemistry to map velocity-to-position neural integrator activity in zebrafish	Lee MM,Arrenberg AB,Aksay ER	The Journal of neuroscience : the official journal of the Society for Neuroscience (35:7903)	2015
Not Applicable	1:500	Imaging activity in astrocytes and neurons with genetically encoded calcium indicators following in utero electroporation. A10262 was used in immunohistochemistry to describe an in utero electroporation method to add genes to study neurons in rats	Gee JM,Gibbons MB,Taheri M,Palumbos S,Morris SC,Smeal RM,Flynn KF,Economo MN,Cizek CG,Capecchi MR,Tvrdik P,Wilcox KS,White JA	Frontiers in molecular neuroscience (8:null)	2015
Mouse	0.2 ug/ml	Structural basis for serotonergic regulation of neural circuits in the mouse olfactory bulb. A10262 was used in immunohistochemistry to identify the mechanism for serotonergic regulation of neural circuits in the mouse olfactory bulb	Suzuki Y,Kiyokage E,Sohn J,Hioki H,Toida K	The Journal of comparative neurology (523:262)	2015
Not Applicable	1:1000	Bidirectional switch of the valence associated with a hippocampal contextual memory engram. A10262 was used in immunohistochemistry to assess the bidirectional switch of the valence associated with a contextual memory engram of the hippocampal region	Redondo RL,Kim J,Arons AL,Ramirez S,Liu X,Tonegawa S	Nature (513:426)	2014
Not Applicable	1:500	Differential responsiveness of distinct retinal domains to Atoh7. A10262 was used in immunohistochemistry to explore the differential susceptibilities of early retinal progenitor cells to Atoh7 in vivo	Sinn R,Peravali R,Heermann S,Wittbrodt J	Mechanisms of development (133:218)	2014
Not Applicable	1:1,500	Atg17/FIP200 localizes to perilysosomal Ref(2)P aggregates and promotes autophagy by activation of Atg1 in Drosophila. A10262 was used in immunohistochemistry to study the Atg17/FIP200 complex that localizes to perilysosomal Ref(2)P aggregates and activates Atg1 in Drosophila that promotes autophagy	Nagy P,Kárpáti M,Varga A,Pircs K,Venkei Z,Takáts S,Varga K,Erdi B,Heged¿s K,Juhász G	Autophagy (10:453)	2014
Not Applicable	1:1000	TrkB signaling directs the incorporation of newly generated periglomerular cells in the adult olfactory bulb. A10262 was used in immunohistochemistry to understand the role of TrkB in the integration of new periglomerular cells in the adult olfactory bulb	Bergami M,Vignoli B,Motori E,Pifferi S,Zuccaro E,Menini A,Canossa M	The Journal of neuroscience : the official journal of the Society for Neuroscience (33:11464)	2013
Not Applicable	1:200	Thymosin ß4-sulfoxide attenuates inflammatory cell infiltration and promotes cardiac wound healing. A10262 was used in immunohistochemistry to assess cardiac wound healing and inflammatory cell infiltration is attenuated by thymosin beta-4-sulfoxide	Evans MA,Smart N,Dubé KN,Bollini S,Clark JE,Evans HG,Taams LS,Richardson R,Lévesque M,Martin P,Mills K,Riegler J,Price AN,Lythgoe MF,Riley PR	Nature communications (4:null)	2013
Mouse	Not Cited	Localization of neurones expressing the gap junction protein Connexin45 within the adult spinal dorsal horn: a study using Cx45-eGFP reporter mice. A10262 was used in immunohistochemistry to identify Cx45-positive neurones in the adult spinal dorsal horn	Chapman RJ,Lall VK,Maxeiner S,Willecke K,Deuchars J,King AE	Brain structure and function (218:751)	2013
Not Applicable	Not Cited	An epigenetic feedback regulatory loop involving microRNA-195 and MBD1 governs neural stem cell differentiation. A10262 was used in immunohistochemistry to report that MBD1 deficiency in adult neural stem/progenitor cells results in altered expression of several noncoding microRNAs	Liu C,Teng ZQ,McQuate AL,Jobe EM,Christ CC,von Hoyningen-Huene SJ,Reyes MD,Polich ED,Xing Y,Li Y,Guo W,Zhao X	PloS one (8:null)	2013
Not Applicable	1:1000	Whacked and Rab35 polarize dynein-motor-complex-dependent seamless tube growth. A10262 was used in immunohistochemistry to examine the contribution of vesicle trafficking to seamless tube formation	Schottenfeld-Roames J,Ghabrial AS	Nature cell biology (14:386)	2012
Not Applicable	Not Cited	Nitric oxide induces pathological synapse loss by a protein kinase G-, Rho kinase-dependent mechanism preceded by myosin light chain phosphorylation. A10262 was used in immunohistochemistry to study induction of pathological synapse loss by a protein kinase G-, Rho kinase-dependent mechanism preceded by myosin light chain phosphorylation due to nitric oxide	Sunico CR,González-Forero D,Domínguez G,García-Verdugo JM,Moreno-López B	The Journal of neuroscience : the official journal of the Society for Neuroscience (30:973)	2010

Immunohistochemistry - Free Floating

Species	Dilution	Title	Authors	Journal (Vol:Pg)	Year
Not Applicable	1:1000	Antagonistic negative and positive neurons of the basolateral amygdala. A10262 was used in immunohistochemistry - free floating to identify two genetically distinct, spatially segregated populations of excitatory neurons in the basolateral amygdala that participate in valence-specific behaviors	Kim J,Pignatelli M,Xu S,Itohara S,Tonegawa S	Nature neuroscience (19:1636)	2016
Not Applicable	Not Cited	MicroRNA miR-137 regulates neuronal maturation by targeting ubiquitin ligase mind bomb-1. A10262 was used in immunohistochemistry - free floating to investigate the role of miR-137 in neuronal maturation	Smrt RD,Szulwach KE,Pfeiffer RL,Li X,Guo W,Pathania M,Teng ZQ,Luo Y,Peng J,Bordey A,Jin P,Zhao X	Stem cells (Dayton, Ohio) (28:1060)	2010

Immunocytochemistry

Species	Dilution	Title	Authors	Journal (Vol:Pg)	Year
Not Applicable	1:100	Differential Nanos 2 protein stability results in selective germ cell accumulation in the sea urchin. A10262 was used in immunocytochemistry to study Nanos localization and stability in the sea urchin	Oulhen N,Wessel GM	Developmental biology (418:146)	2016
Not Applicable	1:400	Apoptotic Activity of MeCP2 Is Enhanced by C-Terminal Truncating Mutations. A10262 was used in immunocytochemistry study the function of the MeCP2 C-terminus	Williams AA,Mehler VJ,Mueller C,Vonhoff F,White R,Duch C	PloS one (11:null)	2016
Not Applicable	Not Cited	CDKL5 and Shootin1 Interact and Concur in Regulating Neuronal Polarization. A10262 was used in immunocytochemistry to characterize the interaction between Shootin1 and CDKL5 and how they work together to regulate neuronal polarization	Nawaz MS,Giarda E,Bedogni F,La Montanara P,Ricciardi S,Ciceri D,Alberio T,Landsberger N,Rusconi L,Kilstrup-Nielsen C	PloS one (11:null)	2016
Not Applicable	Not Cited	Reduced ciliary polycystin-2 in induced pluripotent stem cells from polycystic kidney disease patients with PKD1 mutations. A10262 was used in immunocytochemistry to investigate the relationship between pathopolycystin- and polycystin-2 in the pathogenesis of PKD.	Freedman BS,Lam AQ,Sundsbak JL,Iatrino R,Su X,Koon SJ,Wu M,Daheron L,Harris PC,Zhou J,Bonventre JV	Journal of the American Society of Nephrology : JASN (24:1571)	2013
Not Applicable	Not Cited	CD2AP links cortactin and capping protein at the cell periphery to facilitate formation of lamellipodia. A10262 was used in immunocytochemistry to analyze the facilitation of lamellipodia formation by CD2AP linking capping protein and cortactin at the cell periphery	Zhao J,Bruck S,Cemerski S,Zhang L,Butler B,Dani A,Cooper JA,Shaw AS	Molecular and cellular biology (33:38)	2013
Not Applicable	1:2000	CDKL5 ensures excitatory synapse stability by reinforcing NGL-1-PSD95 interaction in the postsynaptic compartment and is impaired in patient iPSC-derived neurons. A10262 was used in immunocytochemistry to elucidate the function of CDKL5 in neurons.	Ricciardi S,Ungaro F,Hambrock M,Rademacher N,Stefanelli G,Brambilla D,Sessa A,Magagnotti C,Bachi A,Giarda E,Verpelli C,Kilstrup-Nielsen C,Sala C,Kalscheuer VM,Broccoli V	Nature cell biology (14:911)	2012
Not Applicable	1:1000	Maturation of spinal motor neurons derived from human embryonic stem cells. A10262 was used in immunocytochemistry to examine the morphological and electrophysiological changes during the maturation of neurons differentiated from human embryonic stem.	Takazawa T,Croft GF,Amoroso MW,Studer L,Wichterle H,Macdermott AB	PloS one (7:null)	2012
Not Applicable	1:500	Hsa-miR-34b is a plasma-stable microRNA that is elevated in pre-manifest Huntington's disease. A10262 was used in immunocytochemistry to identify miR-34b as a biomarker of Huntington's disease	Gaughwin PM,Ciesla M,Lahiri N,Tabrizi SJ,Brundin P,Björkqvist M	Human molecular genetics (20:2225)	2011
Not Applicable	1:250	Wnt signaling requires sequestration of glycogen synthase kinase 3 inside multivesicular endosomes. A10262 was used in immunocytochemistry to determine the molecular mechanisms by which canonical Wnt signaling suppresses glycogen synthase kinase 3 activity.	Taelman VF,Dobrowolski R,Plouhinec JL,Fuentealba LC,Vorwald PP,Gumper I,Sabatini DD,De Robertis EM	Cell (143:1136)	2010
Not Applicable	1:500	Cross talk between microRNA and epigenetic regulation in adult neurogenesis. A10262 was used in immunocytochemistry to investigate the cross talk between miR-137 and epigenetic regulation in adult neural stem cells.	Szulwach KE,Li X,Smrt RD,Li Y,Luo Y,Lin L,Santistevan NJ,Li W,Zhao X,Jin P	The Journal of cell biology (189:127)	2010

Immunohistochemistry (Paraffin)

Species	Dilution	Title	Authors	Journal (Vol:Pg)	Year
Not Applicable	1:1000	Expression of Activated Ras in Gastric Chief Cells of Mice Leads to the Full Spectrum of Metaplastic Lineage Transitions. A10262 was used in immunohistochemistry - paraffin section to test the expression of activated Ras in gastric chief cells in mice and the metaplastic lineage transitions	Choi E,Hendley AM,Bailey JM,Leach SD,Goldenring JR	Gastroenterology (150:918)	2016
Not Applicable	Not Cited	Niche appropriation by Drosophila intestinal stem cell tumours. A10262 was used in immunohistochemistry - paraffin section to use Drosophilia to investigate how loss of differentiation initiates tumorigenesis	Patel PH,Dutta D,Edgar BA	Nature cell biology (17:1182)	2015
Not Applicable	1:1000	notch3 is essential for oligodendrocyte development and vascular integrity in zebrafish. A10262 was used in immunohistochemistry - paraffin section to study how Notch3 regulates oligodendrocyte precursor cell development	Zaucker A,Mercurio S,Sternheim N,Talbot WS,Marlow FL	Disease models and mechanisms (6:1246)	2013
Not Applicable	1:100	Adult zebrafish as a model system for cutaneous wound-healing research. A10262 was used in immunohistochemistry - paraffin section to develop and characterize a zebrafish model of skin repair	Richardson R,Slanchev K,Kraus C,Knyphausen P,Eming S,Hammerschmidt M	The Journal of investigative dermatology (133:1655)	2013
Not Applicable	1:400	Pou5f1-dependent EGF expression controls E-cadherin endocytosis, cell adhesion, and zebrafish epiboly movements. A10262 was used in immunohistochemistry - paraffin section to study the role of E-cadherin endosomal trafficking to development of zebrafish	Song S,Eckerle S,Onichtchouk D,Marrs JA,Nitschke R,Driever W	Developmental cell (24:486)	2013
Not Applicable	Not Cited	Serum response factor is required for cell contact maintenance but dispensable for proliferation in visceral yolk sac endothelium. A10262 was used in immunohistochemistry - paraffin section to determine the role of serum response factor in endothelial function and cell contact interactions in visceral yolk sac tissues	Holtz ML,Misra RP	BMC developmental biology (11:null)	2011

Miscellaneous PubMed

Species	Dilution	Title	Authors	Journal (Vol:Pg)	Year
Not Applicable	1:500	Selective Effects of PDE10A Inhibitors on Striatopallidal Neurons Require Phosphatase Inhibition by DARPP-32(1,2,3). A10262 was used in immunohistochemistry to examine the mechanism of type 10A phosphodiesterase inhibitors.	Polito M,Guiot E,Gangarossa G,Longueville S,Doulazmi M,Valjent E,Hervé D,Girault JA,Paupardin-Tritsch D,Castro LR,Vincent P	eNeuro (2:null)	2015
Not Applicable	1:1000	In Utero Development of Kisspeptin/GnRH Neural Circuitry in Male Mice. A10262 was used in immunohistochemistry - frozen section to examine the role of kisspeptin/GPR54 in the developing brain	Kumar D,Periasamy V,Freese M,Voigt A,Boehm U	Endocrinology (156:3084)	2015
Not Applicable	Not Cited	Trib3 Is Elevated in Parkinson's Disease and Mediates Death in Parkinson's Disease Models. A10262 was used in immunocytochemistry to investigate the contribution of the Trib3 signaling pathway to neuron death	Aimé P,Sun X,Zareen N,Rao A,Berman Z,Volpicelli-Daley L,Bernd P,Crary JF,Levy OA,Greene LA	The Journal of neuroscience : the official journal of the Society for Neuroscience (35:10731)	2015
Not Applicable	1:500	Piezo2 is required for Merkel-cell mechanotransduction. A10262 was used in immunocytochemistry and immunohistochemistry to show that Piezo2 is a Merkel-cell mechanotransduction channel.	Woo SH,Ranade S,Weyer AD,Dubin AE,Baba Y,Qiu Z,Petrus M,Miyamoto T,Reddy K,Lumpkin EA,Stucky CL,Patapoutian A	Nature (509:622)	2014
Not Applicable	1:10000	A unique mouse model for investigating the properties of amyotrophic lateral sclerosis-associated protein TDP-43, by in utero electroporation. A10262 was used in immunohistochemistry to develop a method using in utero electroporation to introduce pathogenic genes into the brain of mice to study amyotrophic lateral sclerosis.	Akamatsu M,Takuma H,Yamashita T,Okada T,Keino-Masu K,Ishii K,Kwak S,Masu M,Tamaoka A	Neuroscience research (77:234)	2013
Not Applicable	1:1000	Development and specification of cerebellar stem and progenitor cells in zebrafish: from embryo to adult. A10262 was used in immunohistochemistry to examine the diverse neural stem and progenitor cell types in the teleost fish brain.	Kaslin J,Kroehne V,Benato F,Argenton F,Brand M	Neural development (8:null)	2013
Not Applicable	Not Cited	Dopaminergic modulation of mitral cells and odor responses in the zebrafish olfactory bulb. A10262 was used in immunohistochemistry (frozen) to examine the role of dopamine in odor processing.	Bundschuh ST,Zhu P,Schärer YP,Friedrich RW	The Journal of neuroscience : the official journal of the Society for Neuroscience (32:6830)	2012
Not Applicable	Not Cited	The ordered architecture of murine ear epidermis is maintained by progenitor cells with random fate. A10262 was used in immunohistochemistry to use lineage tracing to study the origins of the murine epidermis	Doupé DP,Klein AM,Simons BD,Jones PH	Developmental cell (18:317)	2010

Western Blot

Species	Dilution	Title	Authors	Journal (Vol:Pg)	Year
Not Applicable	Not Cited	Functional Effect of Pim1 Depends upon Intracellular Localization in Human Cardiac Progenitor Cells. A10262 was used in western blot to show that subcellular targeting bolsters the cardioprotective effects of Pim1 in primary human cardiac progenitor cells	Samse K,Emathinger J,Hariharan N,Quijada P,Ilves K,Völkers M,Ormachea L,De La Torre A,Orogo AM,Alvarez R,Din S,Mohsin S,Monsanto M,Fischer KM,Dembitsky WP,Gustafsson ÅB,Sussman MA	The Journal of biological chemistry (290:13935)	2015
Not Applicable	1:1000	Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice. A10262 was used in western blot to study adenoviral vectors that target tumor vascular endothelial cells.	Lu ZH,Kaliberov S,Sohn RE,Kaliberova L,Curiel DT,Arbeit JM	PloS one (8:null)	2013
Not Applicable	1:1000	Protein arginine methyltransferase 1 and 8 interact with FUS to modify its sub-cellular distribution and toxicity in vitro and in vivo. A10262 was used in western blot to examine the role of arginine methylation within the pathogenesis of FUS-related amyotrophic lateral sclerosis	Scaramuzzino C,Monaghan J,Milioto C,Lanson NA,Maltare A,Aggarwal T,Casci I,Fackelmayer FO,Pennuto M,Pandey UB	PloS one (8:null)	2013
Not Applicable	Not Cited	Mechanism and function of Vav1 localisation in TCR signalling. A10262 was used in western blot to suggest that Vav1 is recruited to the immunological synapse by binding to SLP76 and that this interaction results in calcium flux	Ksionda O,Saveliev A,Köchl R,Rapley J,Faroudi M,Smith-Garvin JE,Wülfing C,Rittinger K,Carter T,Tybulewicz VL	Journal of cell science (125:5302)	2012

System Message

GFP Tag Polyclonal Antibody

Catalog#: A10262 Invitrogen

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References

Immunohistochemistry (Frozen)

Immunohistochemistry

Immunohistochemistry - Free Floating

Immunocytochemistry

Immunohistochemistry (Paraffin)

Miscellaneous PubMed

Western Blot

Bioinformatics

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