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|Tested species reactivity||Tag|
|Host / Isotype||Chicken / IgY|
|Immunogen||Purified recombinant Green Fluorescent fusion Protein.|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:500 - 1:2000|
|Immunocytochemistry (ICC)||1:200 - 1:1000|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-9533 detects Green Fluorescence Protein in N/A samples.
PA1-9533 has been successfully used in Western blot, ELISA and immunocytochemistry procedures. By Western blot, PA1-9533 detects a 32 kDa protein representing Green Fluorescence Protein.
PA1-9533 immunogen is a purified recombinant green fluorescence protein.
Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.