Proteintech

GFPT1 Polyclonal Antibody

Product Image Gallery

GFPT1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using GFPT1 antibody 14132-1-AP at 1:100 dilution and PE-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

GFPT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14132-1-AP ( GFPT1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

GFPT1 Antibody in Western Blot (WB) — Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14132-1-AP (GFPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

14132-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 8 publications 8 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat, Zebrafish
Published species	Human, Mouse, Rat, Zebrafish
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GFPT1 Fusion Protein Ag5312 (1-350 aa encoded by BC045641) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.29 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MCGIFAYLN YHVPRTRREI LETLIKGLQR LEYRGYDSAG VGFDGGNDKD WEANACKIQL IKKKGKVKAL DEEVHKQQDM DLDIEFDVHL GIAHTRWATH GEPSPVNSHP QRSDKNNEFI VIHNGIITNY KDLKKFLESK GYDFESETDT ETIAKLVKYM YDNRESQDTS FTTLVERVIQ QLEGAFALVF KSVHFPGQAV GTRRGSPLLI GVRSEHKLST DHIPILYRTG KDKKGSCNLS RVDSTTCLFP VEEKAVEYYF ASDASAVIEH TNRVIFLEDD DVAAVVDGRL SIHRIKRTAG DHPGRAVQTL QMELQQIMKG NFSSFMQKEI FEQPESVVNT MRGRVNFDDY T (1-350 aa encoded by BC045641)

Target Information

This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Consumption of a high fat diet promotes protein O-GlcNAcylation in mouse retina via NR4A1-dependent GFAT2 expression.

Biochimica et biophysica acta. Molecular basis of disease

Dai W,Dierschke SK,Toro AL,Dennis MD

Sat Dec 01 00:00:00 EST 2018

GFPT1 deficiency in muscle leads to myasthenia and myopathy in mice.

Human molecular genetics

Issop Y,Hathazi D,Khan MM,Rudolf R,Weis J,Spendiff S,Slater CR,Roos A,Lochmüller H

Sat Sep 15 00:00:00 EDT 2018

GFPT1 deficiency in muscle leads to myasthenia and myopathy in mice.

Human molecular genetics

Issop Y,Hathazi D,Khan MM,Rudolf R,Weis J,Spendiff S,Slater CR,Roos A,Lochmüller H

Sat Sep 15 00:00:00 EDT 2018

A Genetic Model to Study Increased Hexosamine Biosynthetic Flux.

Endocrinology

Hugo SE,Schlegel A

Tue Aug 01 00:00:00 EDT 2017

MPI depletion enhances O-GlcNAcylation of p53 and suppresses the Warburg effect.

eLife

Shtraizent N,DeRossi C,Nayar S,Sachidanandam R,Katz LS,Prince A,Koh AP,Vincek A,Hadas Y,Hoshida Y,Scott DK,Eliyahu E,Freeze HH,Sadler KC,Chu J

Fri Jun 23 00:00:00 EDT 2017

Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.

Glycobiology

Oikari S,Makkonen K,Deen AJ,Tyni I,Kärnä R,Tammi RH,Tammi MI

Fri Jul 01 00:00:00 EDT 2016

A 3'-UTR mutation creates a microRNA target site in the GFPT1 gene of patients with congenital myasthenic syndrome.

Human molecular genetics

Dusl M,Senderek J,Müller JS,Vogel JG,Pertl A,Stucka R,Lochmüller H,David R,Abicht A

Mon Jun 15 00:00:00 EDT 2015

Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro.

American journal of physiology. Cell physiology

Ganguly R,Sahu S,Chavez RJ,Raman P

Thu Jan 15 00:00:00 EST 2015

Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro.

American journal of physiology. Cell physiology

Ganguly R,Sahu S,Chavez RJ,Raman P

Thu Jan 15 00:00:00 EST 2015

Hexosamine biosynthetic pathway mutations cause neuromuscular transmission defect.

American journal of human genetics

Senderek J,Müller JS,Dusl M,Strom TM,Guergueltcheva V,Diepolder I,Laval SH,Maxwell S,Cossins J,Krause S,Muelas N,Vilchez JJ,Colomer J,Mallebrera CJ,Nascimento A,Nafissi S,Kariminejad A,Nilipour Y,Bozorgmehr B,Najmabadi H,Rodolico C,Sieb JP,Steinlein OK,Schlotter B,Schoser B,Kirschner J,Herrmann R,Voit T,Oldfors A,Lindbergh C,Urtizberea A,von der Hagen M,Hübner A,Palace J,Bushby K,Straub V,Beeson D,Abicht A,Lochmüller H

Fri Feb 11 00:00:00 EST 2011

Bioinformatics

Protein Aliases: D-fructose-6-phosphate amidotransferase 1; eartha; GFAT 1; glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1; Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1; glutamine:fructose 6 phosphate amidotransferase 1; Glutamine:fructose-6-phosphate amidotransferase 1; harvest moon; Hexosephosphate aminotransferase 1

Gene Aliases: 2810423A18Rik; AI324119; AI449986; CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1; GFAT1m; GFPT; GFPT1; GFPT1L; hmn; MSLG; unm_z110

UniProt ID: (Human) Q06210, (Rat) P82808, (Mouse) P47856

Entrez Gene ID: (Human) 2673, (Rat) 297417, (Mouse) 14583, (Zebrafish) 567861

Molecular Function: transaminase