Proteintech

GFPT1 Polyclonal Antibody

Product Image Gallery

GFPT1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HepG2 cells, using GFPT1 antibody 14132-1-AP at 1:100 dilution and PE-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

GFPT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14132-1-AP ( GFPT1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

GFPT1 Antibody in Western Blot (WB) — Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14132-1-AP (GFPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Western Blot (WB)

1:500-1:1000

Published Applications

Western Blot (WB)

See 8 publications below

Immunofluorescence (IF)

See 2 publications below

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Published species

Human, Mouse, Rat, Zebrafish

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GFPT1 Fusion Protein Ag5312

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: D-fructose-6-phosphate amidotransferase 1; eartha; GFA; GFAT 1; GFAT1; GFAT1m; GFPT; GFPT1; glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1; Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1; glutamine:fructose 6 phosphate amidotransferase 1; Glutamine:fructose-6-phosphate amidotransferase 1; harvest moon; Hexosephosphate aminotransferase 1

Gene Aliases: 2810423A18Rik; AI324119; AI449986; CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1; GFAT1m; GFPT; GFPT1; GFPT1L; hmn; MSLG; unm_z110

UniProt ID: (Human) Q06210, (Rat) P82808, (Mouse) P47856

Entrez Gene ID: (Human) 2673, (Rat) 297417, (Mouse) 14583, (Zebrafish) 567861

Molecular Function: transaminase transferase