Proteintech

GFPT1 Polyclonal Antibody

View all (10) GFPT1 antibodies

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GFPT1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using GFPT1 antibody 14132-1-AP at 1:100 dilution and PE-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

GFPT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14132-1-AP ( GFPT1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

GFPT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14132-1-AP ( GFPT1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

GFPT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using 14132-1-AP (GFPT1 Antibody) at dilution of 1:50 (under 40x lens).

GFPT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using 14132-1-AP (GFPT1 Antibody) at dilution of 1:50 (under 10x lens).

GFPT1 Antibody in Western Blot (WB) — Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14132-1-AP (GFPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

GFPT1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 14132-1-AP (GFPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

14132-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Not applicable (N/A)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GFPT1 Fusion Protein Ag5312 (1-350 aa encoded by BC045641)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MCGIFAYLN YHVPRTRREI LETLIKGLQR LEYRGYDSAG VGFDGGNDKD WEANACKIQL IKKKGKVKAL DEEVHKQQDM DLDIEFDVHL GIAHTRWATH GEPSPVNSHP QRSDKNNEFI VIHNGIITNY KDLKKFLESK GYDFESETDT ETIAKLVKYM YDNRESQDTS FTTLVERVIQ QLEGAFALVF KSVHFPGQAV GTRRGSPLLI GVRSEHKLST DHIPILYRTG KDKKGSCNLS RVDSTTCLFP VEEKAVEYYF ASDASAVIEH TNRVIFLEDD DVAAVVDGRL SIHRIKRTAG DHPGRAVQTL QMELQQIMKG NFSSFMQKEI FEQPESVVNT MRGRVNFDDY T (1-350 aa encoded by BC045641)

Target Information

This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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[WITHDRAWN] Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction.

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GFPT1 promotes the proliferation of cervical cancer via regulating the ubiquitination and degradation of PTEN.

Carcinogenesis

Li D,Guan M,Cao X,Zha ZQ,Zhang P,Xiang H,Zhou Y,Peng Q,Xu Z,Lu L,Liu G

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GFPT1 promotes the proliferation of cervical cancer via regulating the ubiquitination and degradation of PTEN.

Carcinogenesis

Li D,Guan M,Cao X,Zha ZQ,Zhang P,Xiang H,Zhou Y,Peng Q,Xu Z,Lu L,Liu G

Wed Nov 23 00:00:00 EST 2022

GFPT1 promotes the proliferation of cervical cancer via regulating the ubiquitination and degradation of PTEN.

Carcinogenesis

Li D,Guan M,Cao X,Zha ZQ,Zhang P,Xiang H,Zhou Y,Peng Q,Xu Z,Lu L,Liu G

Wed Nov 23 00:00:00 EST 2022

GFPT1 promotes the proliferation of cervical cancer via regulating the ubiquitination and degradation of PTEN.

Carcinogenesis

Li D,Guan M,Cao X,Zha ZQ,Zhang P,Xiang H,Zhou Y,Peng Q,Xu Z,Lu L,Liu G

Wed Nov 23 00:00:00 EST 2022

GFPT1 promotes the proliferation of cervical cancer via regulating the ubiquitination and degradation of PTEN.

Carcinogenesis

Li D,Guan M,Cao X,Zha ZQ,Zhang P,Xiang H,Zhou Y,Peng Q,Xu Z,Lu L,Liu G

Wed Nov 23 00:00:00 EST 2022

GFPT1 promotes the proliferation of cervical cancer via regulating the ubiquitination and degradation of PTEN.

Carcinogenesis

Li D,Guan M,Cao X,Zha ZQ,Zhang P,Xiang H,Zhou Y,Peng Q,Xu Z,Lu L,Liu G

Wed Nov 23 00:00:00 EST 2022

GFPT1 promotes the proliferation of cervical cancer via regulating the ubiquitination and degradation of PTEN.

Carcinogenesis

Li D,Guan M,Cao X,Zha ZQ,Zhang P,Xiang H,Zhou Y,Peng Q,Xu Z,Lu L,Liu G

Wed Nov 23 00:00:00 EST 2022

GFPT1 promotes the proliferation of cervical cancer via regulating the ubiquitination and degradation of PTEN.

Carcinogenesis

Li D,Guan M,Cao X,Zha ZQ,Zhang P,Xiang H,Zhou Y,Peng Q,Xu Z,Lu L,Liu G

Wed Nov 23 00:00:00 EST 2022

GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation.

Cell discovery

Wei S,Zhao Q,Zheng K,Liu P,Sha N,Li Y,Ma C,Li J,Zhuo L,Liu G,Liang W,Jiang Y,Chen T,Zhong N

Tue Aug 09 00:00:00 EDT 2022

GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation.

Cell discovery

Wei S,Zhao Q,Zheng K,Liu P,Sha N,Li Y,Ma C,Li J,Zhuo L,Liu G,Liang W,Jiang Y,Chen T,Zhong N

Tue Aug 09 00:00:00 EDT 2022

GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation.

Cell discovery

Wei S,Zhao Q,Zheng K,Liu P,Sha N,Li Y,Ma C,Li J,Zhuo L,Liu G,Liang W,Jiang Y,Chen T,Zhong N

Tue Aug 09 00:00:00 EDT 2022

GFPT1-Associated Congenital Myasthenic Syndrome Mimicking a Glycogen Storage Disease - Diagnostic Pitfalls in Myopathology Solved by Next-Generation-Sequencing.

Journal of neuromuscular diseases

Mensch A,Cordts I,Scholle L,Joshi PR,Kleeberg K,Emmer A,Beck-Woedl S,Park J,Haack TB,Stoltenburg-Didinger G,Zierz S,Deschauer M

Wed Jul 06 00:00:00 EDT 2022

GFPT1-Associated Congenital Myasthenic Syndrome Mimicking a Glycogen Storage Disease - Diagnostic Pitfalls in Myopathology Solved by Next-Generation-Sequencing.

Journal of neuromuscular diseases

Mensch A,Cordts I,Scholle L,Joshi PR,Kleeberg K,Emmer A,Beck-Woedl S,Park J,Haack TB,Stoltenburg-Didinger G,Zierz S,Deschauer M

Wed Jul 06 00:00:00 EDT 2022

O-GlcNAc modification mediates aquaporin 3 to coordinate endometrial cell glycolysis and affects embryo implantation.

Journal of advanced research

Zhang H,Qi J,Pei J,Zhang M,Shang Y,Li Z,Wang Y,Guo J,Sun K,Fan J,Sui L,Xu Y,Kong L,Kong Y

Tue Mar 01 00:00:00 EST 2022

DOT1L O-GlcNAcylation promotes its protein stability and MLL-fusion leukemia cell proliferation.

Cell reports

Song T,Zou Q,Yan Y,Lv S,Li N,Zhao X,Ma X,Liu H,Tang B,Sun L

Tue Sep 21 00:00:00 EDT 2021

Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.

Redox biology

Ou W,Liang Y,Qin Y,Wu W,Xie M,Zhang Y,Zhang Y,Ji L,Yu H,Li T

Thu Jul 01 00:00:00 EDT 2021

Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.

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Hydrogen sulfide accumulates LDL receptor precursor via downregulating PCSK9 in HepG2 cells.

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Insulin Resistance Does Not Impair Mechanical Overload-Stimulated Glucose Uptake, but Does Alter the Metabolic Fate of Glucose in Mouse Muscle.

International journal of molecular sciences

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Mitochondrial Protein Poldip2 (Polymerase Delta Interacting Protein 2) Controls Vascular Smooth Muscle Differentiated Phenotype by O-Linked GlcNAc (N-Acetylglucosamine) Transferase-Dependent Inhibition of a Ubiquitin Proteasome System.

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Targeting tumor-intrinsic hexosamine biosynthesis sensitizes pancreatic cancer to anti-PD1 therapy.

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Thu Jan 02 00:00:00 EST 2020

Ginsenoside-Rb1 Improved Diabetic Cardiomyopathy through Regulating Calcium Signaling by Alleviating Protein O-GlcNAcylation.

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Consumption of a high fat diet promotes protein O-GlcNAcylation in mouse retina via NR4A1-dependent GFAT2 expression.

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Consumption of a high fat diet promotes protein O-GlcNAcylation in mouse retina via NR4A1-dependent GFAT2 expression.

Biochimica et biophysica acta. Molecular basis of disease

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GFPT1 deficiency in muscle leads to myasthenia and myopathy in mice.

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Issop Y,Hathazi D,Khan MM,Rudolf R,Weis J,Spendiff S,Slater CR,Roos A,Lochmüller H

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GFPT1 deficiency in muscle leads to myasthenia and myopathy in mice.

Human molecular genetics

Issop Y,Hathazi D,Khan MM,Rudolf R,Weis J,Spendiff S,Slater CR,Roos A,Lochmüller H

Sat Sep 15 00:00:00 EDT 2018

GFPT1 deficiency in muscle leads to myasthenia and myopathy in mice.

Human molecular genetics

Issop Y,Hathazi D,Khan MM,Rudolf R,Weis J,Spendiff S,Slater CR,Roos A,Lochmüller H

Sat Sep 15 00:00:00 EDT 2018

GFPT1 deficiency in muscle leads to myasthenia and myopathy in mice.

Human molecular genetics

Issop Y,Hathazi D,Khan MM,Rudolf R,Weis J,Spendiff S,Slater CR,Roos A,Lochmüller H

Sat Sep 15 00:00:00 EDT 2018

A Genetic Model to Study Increased Hexosamine Biosynthetic Flux.

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Tue Aug 01 00:00:00 EDT 2017

A Genetic Model to Study Increased Hexosamine Biosynthetic Flux.

Endocrinology

Hugo SE,Schlegel A

Tue Aug 01 00:00:00 EDT 2017

MPI depletion enhances O-GlcNAcylation of p53 and suppresses the Warburg effect.

eLife

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Fri Jun 23 00:00:00 EDT 2017

MPI depletion enhances O-GlcNAcylation of p53 and suppresses the Warburg effect.

eLife

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Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.

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Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.

Glycobiology

Oikari S,Makkonen K,Deen AJ,Tyni I,Kärnä R,Tammi RH,Tammi MI

Fri Jul 01 00:00:00 EDT 2016

A 3'-UTR mutation creates a microRNA target site in the GFPT1 gene of patients with congenital myasthenic syndrome.

Human molecular genetics

Dusl M,Senderek J,Müller JS,Vogel JG,Pertl A,Stucka R,Lochmüller H,David R,Abicht A

Mon Jun 15 00:00:00 EDT 2015

A 3'-UTR mutation creates a microRNA target site in the GFPT1 gene of patients with congenital myasthenic syndrome.

Human molecular genetics

Dusl M,Senderek J,Müller JS,Vogel JG,Pertl A,Stucka R,Lochmüller H,David R,Abicht A

Mon Jun 15 00:00:00 EDT 2015

Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro.

American journal of physiology. Cell physiology

Ganguly R,Sahu S,Chavez RJ,Raman P

Thu Jan 15 00:00:00 EST 2015

Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro.

American journal of physiology. Cell physiology

Ganguly R,Sahu S,Chavez RJ,Raman P

Thu Jan 15 00:00:00 EST 2015

Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro.

American journal of physiology. Cell physiology

Ganguly R,Sahu S,Chavez RJ,Raman P

Thu Jan 15 00:00:00 EST 2015

Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro.

American journal of physiology. Cell physiology

Ganguly R,Sahu S,Chavez RJ,Raman P

Thu Jan 15 00:00:00 EST 2015

Hexosamine biosynthetic pathway mutations cause neuromuscular transmission defect.

American journal of human genetics

Senderek J,Müller JS,Dusl M,Strom TM,Guergueltcheva V,Diepolder I,Laval SH,Maxwell S,Cossins J,Krause S,Muelas N,Vilchez JJ,Colomer J,Mallebrera CJ,Nascimento A,Nafissi S,Kariminejad A,Nilipour Y,Bozorgmehr B,Najmabadi H,Rodolico C,Sieb JP,Steinlein OK,Schlotter B,Schoser B,Kirschner J,Herrmann R,Voit T,Oldfors A,Lindbergh C,Urtizberea A,von der Hagen M,Hübner A,Palace J,Bushby K,Straub V,Beeson D,Abicht A,Lochmüller H

Fri Feb 11 00:00:00 EST 2011

Hexosamine biosynthetic pathway mutations cause neuromuscular transmission defect.

American journal of human genetics

Senderek J,Müller JS,Dusl M,Strom TM,Guergueltcheva V,Diepolder I,Laval SH,Maxwell S,Cossins J,Krause S,Muelas N,Vilchez JJ,Colomer J,Mallebrera CJ,Nascimento A,Nafissi S,Kariminejad A,Nilipour Y,Bozorgmehr B,Najmabadi H,Rodolico C,Sieb JP,Steinlein OK,Schlotter B,Schoser B,Kirschner J,Herrmann R,Voit T,Oldfors A,Lindbergh C,Urtizberea A,von der Hagen M,Hübner A,Palace J,Bushby K,Straub V,Beeson D,Abicht A,Lochmüller H

Fri Feb 11 00:00:00 EST 2011

Bioinformatics

Protein Aliases: D-fructose-6-phosphate amidotransferase 1; GFAT 1; glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1; Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1; glutamine:fructose 6 phosphate amidotransferase 1; Glutamine:fructose-6-phosphate amidotransferase 1; Hexosephosphate aminotransferase 1

Gene Aliases: 2810423A18Rik; AI324119; AI449986; CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1; GFAT1m; GFPT; GFPT1; GFPT1L; MSLG

UniProt ID: (Human) Q06210, (Rat) P82808, (Mouse) P47856

Entrez Gene ID: (Human) 2673, (Rat) 297417, (Mouse) 14583

Molecular Function: transaminase

carbohydrate metabolic process fructose 6-phosphate metabolic process glucosamine biosynthetic process UDP-N-acetylglucosamine metabolic process UDP-N-acetylglucosamine biosynthetic process energy reserve metabolic process glutamine metabolic process response to sucrose cellular response to insulin stimulus circadian regulation of gene expression IRE1-mediated unfolded protein response negative regulation of glycogen biosynthetic process protein homotetramerization carbohydrate biosynthetic process response to insulin metabolic process rhythmic process carbohydrate derivative biosynthetic process

Process(es)

glutamine-fructose-6-phosphate transaminase (isomerizing) activity amino acid binding carbohydrate binding transaminase activity transferase activity