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Invitrogen
GFPT1 Polyclonal Antibody
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FIGURE: 1 / 6
GFPT1 Antibody (PA5-119759) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of GFPT1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with GFPT1 Polyclonal Antibody (Product # PA5-119759) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
Product Details
PA5-119759
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human GFPT1 aa 1-200.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: 293 cell lysate, rat testis tissue lysate, mouse liver tissue lysate, LOVO, HepG2, rat kidney tissue, human liver tissue, mouse skeletal muscle tissue.
Subcellular Location: cytosol, extracellular exosome.
Target Information
This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: D-fructose-6-phosphate amidotransferase 1; GFAT 1; glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1; Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1; glutamine:fructose 6 phosphate amidotransferase 1; Glutamine:fructose-6-phosphate amidotransferase 1; Hexosephosphate aminotransferase 1
Gene Aliases: 2810423A18Rik; AI324119; AI449986; CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1; GFAT1m; GFPT; GFPT1; GFPT1L; MSLG
UniProt ID: (Human) Q06210, (Rat) P82808, (Mouse) P47856
Entrez Gene ID: (Human) 2673, (Rat) 297417, (Mouse) 14583
carbohydrate metabolic process
fructose 6-phosphate metabolic process
glucosamine biosynthetic process
UDP-N-acetylglucosamine metabolic process
UDP-N-acetylglucosamine biosynthetic process
energy reserve metabolic process
glutamine metabolic process
response to sucrose
cellular response to insulin stimulus
circadian regulation of gene expression
IRE1-mediated unfolded protein response
negative regulation of glycogen biosynthetic process
protein homotetramerization
carbohydrate biosynthetic process
response to insulin
metabolic process
rhythmic process
carbohydrate derivative biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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