Invitrogen

GGT1 Polyclonal Antibody

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GGT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of GGT1/gamma-Glutamyltransferase 1 in paraffin-embedded PC13 xenograft using a GGT1/gamma-Glutamyltransferase 1 polyclonal antibody (Product # PA5-21344) at a dilution of 1:100.

GGT1 Antibody in Western Blot (WB) — Western blot analysis of GGT1/gamma-Glutamyltransferase 1 using A) 30 µg Neuro2A whole cell lysate (B) 30 µg GL261 whole cell lysate (C) 30 µg C8D30 whole cell lysate (D) 30 µg NIH-3T3 whole cell lysate (E) 30 µg Raw264.7 whole cell lysate and F) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a GGT1/gamma-Glutamyltransferase 1 polyclonal antibody (Product # PA5-21344) at a dilution of 1:1000.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

View 1 publication 1 publication

Western Blot (WB)

1:500-1:3000

Product Specifications

Species

Human, Mouse

Published species

Human

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 226 of GGT1 (Uniprot ID#P19440)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11152897

Product Specific Information

Recommended positive controls: Molt-4, Neuro2A, GL261, C8D30, NIH-3T3, Raw264.7, C2C12.

Predicted reactivity: Mouse (81%), Rat (80%), Pig (85%), Bovine (82%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Glutamyltransferase (gGT) from human kidney is a membrane-bound enzyme that transfers the gamma-glutamly moiety of gamma-glutamyl compounds such as glutathione to an acceptor, which may be an amino acid or a di- or tripeptide or water. The principal functions of gGT may be hydrolysis and metabolism of glutathione. Various human gGT isoforms have been found. The molecular mass of gGT for normal kidney is 90kDa and gGT can be used as a renal cell marker.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD224; Gamma-glutamyltransferase 1; Gamma-glutamyltranspeptidase 1; GGT 1; glutathione hydrolase 1; Glutathione hydrolase 1 heavy chain; Glutathione hydrolase 1 light chain; Glutathione hydrolase 1 proenzyme; Leukotriene-C4 hydrolase; testicular tissue protein Li 73

Gene Aliases: CD224; D22S672; D22S732; dwg; GGT; GGT 1; GGT-1; GGT1; Ggtp; GTG

UniProt ID: (Human) P19440, (Mouse) Q60928

Entrez Gene ID: (Human) 2678, (Mouse) 14598

Molecular Function: acyltransferase hydrolase protease transferase