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Invitrogen
GHSR Polyclonal Antibody
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FIGURE: 1 / 9
GHSR Antibody (PA5-28752) in ICC/IF
Immunofluorescence analysis of GHSR was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GHSR Polyclonal Antibody (Product # PA5-28752) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034)... View More
Product Details
PA5-28752
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide encompassing a sequence within the Extracellular domain of human GHS-R1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH, mouse hypothalamus, mouse brain, rat brain.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Growth hormone secretagogue receptor 1a (GHSR) is a member of the G-protein coupled receptor family. GHSR may play a role in energy homeostasis and regulation of body weight. Two identified transcript variants are expressed in several tissues and are evolutionary conserved in fish and swine. One transcript, 1a, excises an intron and encodes the functional protein; this protein is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. The second transcript (1b) retains the intron and does not function as a receptor for Ghrelin; however, it may function to attenuate activity of isoform 1a. Mutations to the gene can cause isolated partial growth hormone deficiency.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: G-protein coupled receptor; GH-releasing peptide receptor; Ghrelin receptor; GHRP; Ghrp receptor; GHS receptor; GHS-R; Growth hormone secretagogue receptor type 1
Gene Aliases: C530020I22Rik; GHDP; GHS-R1a; GHSR; GHSR-1a
UniProt ID: (Human) Q92847, (Mouse) Q99P50
Entrez Gene ID: (Human) 2693, (Mouse) 208188
G-protein coupled receptor internalization
signal transduction
G-protein coupled receptor signaling pathway
spermatogenesis
female pregnancy
learning or memory
actin polymerization or depolymerization
adult feeding behavior
response to hormone
hormone-mediated signaling pathway
negative regulation of norepinephrine secretion
peptide hormone secretion
growth hormone secretion
response to food
negative regulation of appetite
positive regulation of appetite
response to follicle-stimulating hormone
response to estradiol
negative regulation of interleukin-1 beta production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
cellular response to insulin stimulus
cellular response to hormone stimulus
ghrelin secretion
positive regulation of multicellular organism growth
regulation of hindgut contraction
positive regulation of insulin-like growth factor receptor signaling pathway
positive regulation of fatty acid metabolic process
positive regulation of growth
negative regulation of insulin secretion
decidualization
insulin-like growth factor receptor signaling pathway
negative regulation of inflammatory response
regulation of synapse assembly
regulation of transmission of nerve impulse
regulation of growth hormone secretion
regulation of feeding behavior
response to growth hormone
cellular response to lipopolysaccharide
response to dexamethasone
negative regulation of locomotion involved in locomotory behavior
cellular response to thyroid hormone stimulus
regulation of neurotransmitter receptor localization to postsynaptic specialization membrane
postsynaptic modulation of chemical synaptic transmission
regulation of postsynapse organization
positive regulation of small intestinal transit
response to L-glutamate
positive regulation of sprouting angiogenesis
positive regulation of eating behavior
positive regulation of small intestine smooth muscle contraction
regulation of gastric motility
positive regulation of vascular endothelial cell proliferation
cellular response to insulin-like growth factor stimulus
negative regulation of macrophage apoptotic process
negative regulation of tumor necrosis factor biosynthetic process
negative regulation of interleukin-6 biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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